Rubritalea squalenifaciens DSM 18772
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3805 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6ASZ7|A0A1M6ASZ7_9BACT N5-carboxyaminoimidazole ribonucleotide mutase OS=Rubritalea squalenifaciens DSM 18772 OX=1123071 GN=purE PE=3 SV=1
MM1 pKa = 7.27 TPLIAFAQTLPDD13 pKa = 3.96 SLVLNLDD20 pKa = 3.27 TDD22 pKa = 4.5 GNQTAEE28 pKa = 4.24 TTVNLTKK35 pKa = 10.52 RR36 pKa = 11.84 SVRR39 pKa = 11.84 APGVAYY45 pKa = 10.66 YY46 pKa = 10.48 RR47 pKa = 11.84 FNGNSQTYY55 pKa = 8.14 TLSPNDD61 pKa = 3.24 IPQVRR66 pKa = 11.84 TYY68 pKa = 10.41 RR69 pKa = 11.84 GTVAGDD75 pKa = 3.43 PNVIVCATILPDD87 pKa = 3.6 GTIKK91 pKa = 10.86 ADD93 pKa = 3.91 AFDD96 pKa = 3.87 HH97 pKa = 6.19 LKK99 pKa = 10.52 GRR101 pKa = 11.84 GYY103 pKa = 9.14 VWQVSGYY110 pKa = 10.32 DD111 pKa = 3.36 VSASLNQGGAATANPTRR128 pKa = 11.84 PEE130 pKa = 4.06 GGMGEE135 pKa = 4.36 LPMGVDD141 pKa = 3.16 INYY144 pKa = 9.28 HH145 pKa = 5.35 RR146 pKa = 11.84 FVNEE150 pKa = 3.92 GASDD154 pKa = 4.28 PDD156 pKa = 3.87 LAWALTEE163 pKa = 4.46 HH164 pKa = 6.54 NFNIYY169 pKa = 10.65 DD170 pKa = 3.72 LMMGRR175 pKa = 11.84 DD176 pKa = 3.22 TGVAVSIATLVTRR189 pKa = 11.84 EE190 pKa = 3.82 NQEE193 pKa = 4.14 YY194 pKa = 9.79 YY195 pKa = 11.07 VPTGSGNHH203 pKa = 5.73 LNIIEE208 pKa = 4.75 AEE210 pKa = 3.94 WSDD213 pKa = 3.24 AAKK216 pKa = 10.22 PLRR219 pKa = 11.84 NAAWEE224 pKa = 4.11 QIFSYY229 pKa = 8.52 KK230 pKa = 10.02 TSVGLFGTGGYY241 pKa = 9.69 AWQNRR246 pKa = 11.84 IGKK249 pKa = 9.08 NEE251 pKa = 4.23 TINYY255 pKa = 7.07 GQGAIGTQTLYY266 pKa = 10.95 HH267 pKa = 6.81 EE268 pKa = 5.02 SGGHH272 pKa = 5.2 NWNAHH277 pKa = 3.63 HH278 pKa = 6.96 WGYY281 pKa = 11.25 GRR283 pKa = 11.84 DD284 pKa = 3.5 AMGSNRR290 pKa = 11.84 PNHH293 pKa = 5.98 GPYY296 pKa = 9.33 NVEE299 pKa = 3.79 RR300 pKa = 11.84 VRR302 pKa = 11.84 EE303 pKa = 3.93 KK304 pKa = 10.88 RR305 pKa = 11.84 DD306 pKa = 3.13 IKK308 pKa = 10.85 IADD311 pKa = 3.74 GTLLQPNGDD320 pKa = 4.26 YY321 pKa = 10.53 PSPAHH326 pKa = 6.78 PYY328 pKa = 9.91 SHH330 pKa = 7.57 LDD332 pKa = 3.35 LATTNVNQAVTISPLVNDD350 pKa = 3.89 TDD352 pKa = 4.35 FNGDD356 pKa = 3.75 SISIVSFTATTANGGTVVQSGNDD379 pKa = 3.9 LIYY382 pKa = 10.05 TPPAGYY388 pKa = 9.71 VGKK391 pKa = 10.22 DD392 pKa = 3.05 IIAYY396 pKa = 8.51 TIEE399 pKa = 4.65 DD400 pKa = 3.5 NSGAGDD406 pKa = 3.81 GGANLQAQDD415 pKa = 4.49 LIFIEE420 pKa = 4.64 VVNNGLTLKK429 pKa = 10.63 FDD431 pKa = 4.41 FNDD434 pKa = 3.06 VSGASVSNSTGLGHH448 pKa = 6.62 SAVLQSSNIEE458 pKa = 4.21 SQLTTGPDD466 pKa = 3.49 GYY468 pKa = 11.52 ALVVPSGGLLVDD480 pKa = 4.37 DD481 pKa = 4.87 TNILPIPYY489 pKa = 8.86 TGSTNNFYY497 pKa = 10.48 PFDD500 pKa = 4.68 SDD502 pKa = 4.91 QMSQGNFFDD511 pKa = 5.7 PMDD514 pKa = 4.67 KK515 pKa = 10.69 DD516 pKa = 3.68 YY517 pKa = 11.42 AICLWIKK524 pKa = 9.88 MDD526 pKa = 5.08 DD527 pKa = 4.15 VITKK531 pKa = 10.46 LDD533 pKa = 3.44 ILDD536 pKa = 3.78 KK537 pKa = 11.29 YY538 pKa = 10.03 KK539 pKa = 10.86 AQEE542 pKa = 3.94 RR543 pKa = 11.84 STGFHH548 pKa = 6.4 LFVEE552 pKa = 4.37 NGVLKK557 pKa = 10.54 ADD559 pKa = 3.83 CGEE562 pKa = 4.01 IEE564 pKa = 4.62 GIYY567 pKa = 10.28 NKK569 pKa = 10.36 RR570 pKa = 11.84 SITNGGAVTAGQWHH584 pKa = 6.29 HH585 pKa = 6.12 VALVFDD591 pKa = 4.38 RR592 pKa = 11.84 TADD595 pKa = 3.35 QMKK598 pKa = 10.1 IFLDD602 pKa = 4.73 GAQTAQTALAAGSFIFHH619 pKa = 6.13 GRR621 pKa = 11.84 EE622 pKa = 3.43 PLMIGSGSGGTMAVDD637 pKa = 3.96 DD638 pKa = 4.2 VRR640 pKa = 11.84 LYY642 pKa = 9.08 TQNLSSSDD650 pKa = 3.19 LTGIMGEE657 pKa = 3.96 ASNAVDD663 pKa = 4.54 EE664 pKa = 4.64 LALNNSLQTWWALDD678 pKa = 3.81 DD679 pKa = 4.08 NAGATASDD687 pKa = 3.37 SSGNANAGALSGGAAWTTTAVDD709 pKa = 3.29 GGGASFDD716 pKa = 3.95 GVDD719 pKa = 5.02 DD720 pKa = 5.29 KK721 pKa = 11.64 IKK723 pKa = 11.05 LDD725 pKa = 3.86 LAQSNVGAFTVAMWAKK741 pKa = 10.42 SGADD745 pKa = 3.47 GQGVYY750 pKa = 10.76 SSVFSNRR757 pKa = 11.84 TPNTGGTFQIDD768 pKa = 3.52 MGNGFQYY775 pKa = 10.44 RR776 pKa = 11.84 GSQTSSFGAAPLDD789 pKa = 3.72 QWVHH793 pKa = 5.7 LAVVSDD799 pKa = 3.78 GTDD802 pKa = 3.09 TILYY806 pKa = 9.46 YY807 pKa = 10.72 NGEE810 pKa = 4.04 IAKK813 pKa = 10.03 VLSGVNDD820 pKa = 4.09 DD821 pKa = 4.84 LFNALVLGANRR832 pKa = 11.84 NEE834 pKa = 3.86 GDD836 pKa = 3.82 FFQGEE841 pKa = 4.19 IDD843 pKa = 3.8 DD844 pKa = 3.85 FCYY847 pKa = 9.74 FDD849 pKa = 4.21 RR850 pKa = 11.84 ALNPSEE856 pKa = 3.6 IQYY859 pKa = 10.44 IKK861 pKa = 10.92 SSLLKK866 pKa = 10.4 NVAPSASDD874 pKa = 3.1 DD875 pKa = 3.57 TFAVVEE881 pKa = 4.11 NASAGTSLGVVNASDD896 pKa = 4.18 PNSGDD901 pKa = 3.72 VVSHH905 pKa = 7.3 AITGGNAAGLFAITAATGEE924 pKa = 4.31 ITTTAPLDD932 pKa = 3.64 YY933 pKa = 8.82 EE934 pKa = 4.53 TASSYY939 pKa = 11.54 VLTVTVSDD947 pKa = 4.83 DD948 pKa = 3.57 GGLSDD953 pKa = 4.08 TATITVNVTDD963 pKa = 4.03 VANDD967 pKa = 3.69 DD968 pKa = 3.93 SDD970 pKa = 5.71 ADD972 pKa = 3.82 GLTDD976 pKa = 3.17 EE977 pKa = 4.85 WEE979 pKa = 4.17 VANFGSVAATTGSADD994 pKa = 3.47 SDD996 pKa = 3.65 GDD998 pKa = 4.08 GYY1000 pKa = 9.95 TNAQEE1005 pKa = 4.19 EE1006 pKa = 4.52 ASATDD1011 pKa = 4.05 PNNAASNPTTQVLAHH1026 pKa = 6.61 WSLNEE1031 pKa = 3.79 GAGLVATDD1039 pKa = 4.15 SSVNGYY1045 pKa = 10.17 DD1046 pKa = 3.52 GQLTGGTAWTSSAVDD1061 pKa = 3.19 AGGASFDD1068 pKa = 4.19 GVDD1071 pKa = 4.66 DD1072 pKa = 5.44 RR1073 pKa = 11.84 IDD1075 pKa = 3.49 YY1076 pKa = 11.27 ALTEE1080 pKa = 4.53 TNLGAYY1086 pKa = 7.22 TVAMWVKK1093 pKa = 10.38 NGASGQSIYY1102 pKa = 11.38 SSVFSNHH1109 pKa = 5.64 TPNTSNTFQIDD1120 pKa = 3.18 MGNGFNYY1127 pKa = 10.4 RR1128 pKa = 11.84 GTQTASFGAAPVGTWVHH1145 pKa = 6.12 LAVVSDD1151 pKa = 3.87 GTDD1154 pKa = 2.67 TRR1156 pKa = 11.84 LYY1158 pKa = 9.65 YY1159 pKa = 10.81 NGVLSQTLAGVNDD1172 pKa = 4.47 DD1173 pKa = 4.83 LFDD1176 pKa = 4.86 TITLGTNRR1184 pKa = 11.84 NEE1186 pKa = 4.11 GNFFQGEE1193 pKa = 3.93 VDD1195 pKa = 3.33 EE1196 pKa = 5.65 LYY1198 pKa = 11.07 VYY1200 pKa = 10.68 DD1201 pKa = 4.9 AALNDD1206 pKa = 3.73 EE1207 pKa = 5.71 DD1208 pKa = 4.65 ILALATPPAPNQPPVATNGSGSVAEE1233 pKa = 4.17 NASVGTVVTTVVASDD1248 pKa = 4.36 PDD1250 pKa = 3.76 TGDD1253 pKa = 3.36 TLSYY1257 pKa = 10.72 SITAGNTGGAFAINSGTGEE1276 pKa = 4.13 VTVAAGLDD1284 pKa = 3.79 FEE1286 pKa = 6.33 SISQYY1291 pKa = 10.74 ILTVEE1296 pKa = 4.19 VSDD1299 pKa = 5.48 GSLTDD1304 pKa = 3.52 TATVTIDD1311 pKa = 3.24 VTNVNEE1317 pKa = 4.67 APVANNGSGSVSEE1330 pKa = 4.47 GASIGATVSTVSSSDD1345 pKa = 3.46 PDD1347 pKa = 3.77 TGDD1350 pKa = 3.14 SATYY1354 pKa = 9.56 TITAGNTGGAFVINSTSGAITTATGLDD1381 pKa = 3.96 YY1382 pKa = 8.91 EE1383 pKa = 4.72 TTALYY1388 pKa = 10.95 SLTVQVTDD1396 pKa = 4.22 GGGLTDD1402 pKa = 3.64 TATVTVNVTDD1412 pKa = 3.75 VANDD1416 pKa = 3.69 DD1417 pKa = 3.93 SDD1419 pKa = 5.71 ADD1421 pKa = 3.82 GLTDD1425 pKa = 3.19 EE1426 pKa = 5.01 WEE1428 pKa = 4.13 VSNFGSVAAQDD1439 pKa = 3.94 GSGDD1443 pKa = 3.91 ADD1445 pKa = 3.36 SDD1447 pKa = 4.36 GYY1449 pKa = 9.84 TNAEE1453 pKa = 3.94 EE1454 pKa = 4.32 FAAGTDD1460 pKa = 3.6 PSDD1463 pKa = 4.29 PLSAPAPPDD1472 pKa = 3.71 LVWTGAAGDD1481 pKa = 3.54 GDD1483 pKa = 4.53 FFNEE1487 pKa = 4.38 ANWDD1491 pKa = 3.73 SDD1493 pKa = 3.44 ALTAGTQVPSADD1505 pKa = 3.36 SVNVNQPLVASSLTVDD1521 pKa = 3.22 SANFIGIGTVIPDD1534 pKa = 3.98 GARR1537 pKa = 11.84 IDD1539 pKa = 3.72 ITNTTLTTSGSNGFNTTSSTLSEE1562 pKa = 4.32 FNIGTGADD1570 pKa = 3.26 VSFQFIANTTVSLAGDD1586 pKa = 3.43 AVLEE1590 pKa = 4.16 FRR1592 pKa = 11.84 GGGNPVNGSTIDD1604 pKa = 3.69 LAADD1608 pKa = 4.01 FAGQVRR1614 pKa = 11.84 FTSEE1618 pKa = 3.74 TVASVLSEE1626 pKa = 4.14 HH1627 pKa = 6.25 MSKK1630 pKa = 8.48 ITVAGQPAVNGVNAYY1645 pKa = 9.76 VVDD1648 pKa = 4.14 VNGVTVVSLEE1658 pKa = 4.15 APNNAPVANDD1668 pKa = 3.24 AAFTVAEE1675 pKa = 4.17 NAGASATVGTVSVSDD1690 pKa = 4.29 PDD1692 pKa = 5.53 AGDD1695 pKa = 2.88 SWTYY1699 pKa = 11.28 AITAGNGGGEE1709 pKa = 4.05 FAINSTTGEE1718 pKa = 4.07 ITTTTSLDD1726 pKa = 3.53 YY1727 pKa = 10.7 EE1728 pKa = 4.66 GASSYY1733 pKa = 11.51 VLTVTVTDD1741 pKa = 3.81 AGGLSDD1747 pKa = 3.74 VATITVDD1754 pKa = 3.2 VLNINEE1760 pKa = 4.16 APVANSASGTVSEE1773 pKa = 4.79 DD1774 pKa = 3.15 VAVGVTVATVTSSDD1788 pKa = 3.4 PDD1790 pKa = 3.79 ANDD1793 pKa = 2.86 TRR1795 pKa = 11.84 TYY1797 pKa = 11.01 AITAGNDD1804 pKa = 2.72 GRR1806 pKa = 11.84 FVINASTGEE1815 pKa = 4.03 ITTVSALDD1823 pKa = 3.72 YY1824 pKa = 7.19 EE1825 pKa = 4.82 TTNSYY1830 pKa = 11.33 SLTVTVTDD1838 pKa = 4.16 AGGLTATAMVAITVTDD1854 pKa = 3.8 VVFEE1858 pKa = 5.34 DD1859 pKa = 4.32 YY1860 pKa = 11.13 DD1861 pKa = 4.67 SDD1863 pKa = 4.57 SLDD1866 pKa = 4.49 DD1867 pKa = 3.49 NWEE1870 pKa = 3.76 IANFGDD1876 pKa = 4.18 TNTSDD1881 pKa = 3.39 GTGDD1885 pKa = 3.6 ADD1887 pKa = 5.38 ADD1889 pKa = 3.82 GLTDD1893 pKa = 3.78 GEE1895 pKa = 4.57 EE1896 pKa = 4.22 YY1897 pKa = 10.9 LAGSDD1902 pKa = 4.24 PNSSDD1907 pKa = 3.15 GDD1909 pKa = 3.43 GDD1911 pKa = 3.89 GFHH1914 pKa = 7.62 DD1915 pKa = 3.71 VLEE1918 pKa = 4.83 INVGTDD1924 pKa = 3.03 PMDD1927 pKa = 3.66 ILDD1930 pKa = 4.86 FPASEE1935 pKa = 4.21 YY1936 pKa = 11.12 DD1937 pKa = 5.19 GLVLWWNLDD1946 pKa = 3.33 EE1947 pKa = 4.66 SAGASTVLDD1956 pKa = 3.45 DD1957 pKa = 4.79 TGNKK1961 pKa = 8.94 MSGAVNGATLNGSEE1975 pKa = 4.52 GSFDD1979 pKa = 3.8 GSNDD1983 pKa = 4.39 SIDD1986 pKa = 4.26 LPLASGG1992 pKa = 3.65
Molecular weight: 205.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.617
IPC_protein 3.694
Toseland 3.439
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.643
Nozaki 3.795
DTASelect 4.088
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.808
Patrickios 1.405
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A1M6M0H0|A0A1M6M0H0_9BACT Thiol-disulfide interchange protein contains DsbC and DsbD domains OS=Rubritalea squalenifaciens DSM 18772 OX=1123071 GN=SAMN02745181_2559 PE=4 SV=1
MM1 pKa = 6.89 STAHH5 pKa = 6.24 EE6 pKa = 4.9 KK7 pKa = 10.06 IVHH10 pKa = 5.81 AALRR14 pKa = 11.84 DD15 pKa = 3.62 ILEE18 pKa = 3.91 QALRR22 pKa = 11.84 RR23 pKa = 11.84 LRR25 pKa = 11.84 VVGGNDD31 pKa = 3.23 LASKK35 pKa = 8.59 VTVVWNSRR43 pKa = 11.84 MRR45 pKa = 11.84 STAGKK50 pKa = 9.59 AHH52 pKa = 6.14 WPEE55 pKa = 4.04 AKK57 pKa = 10.38 VEE59 pKa = 4.33 LNPKK63 pKa = 10.13 LIGISLAEE71 pKa = 4.19 VKK73 pKa = 9.4 RR74 pKa = 11.84 TLLHH78 pKa = 6.27 EE79 pKa = 4.42 LAHH82 pKa = 6.23 LLAYY86 pKa = 9.82 HH87 pKa = 5.1 RR88 pKa = 11.84 HH89 pKa = 4.9 KK90 pKa = 10.54 RR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 IQPHH97 pKa = 4.21 GRR99 pKa = 11.84 EE100 pKa = 4.06 WQQACADD107 pKa = 3.98 LGIPGEE113 pKa = 4.52 SVTHH117 pKa = 6.1 NLALPSSRR125 pKa = 11.84 QRR127 pKa = 11.84 RR128 pKa = 11.84 KK129 pKa = 8.05 WRR131 pKa = 11.84 YY132 pKa = 8.03 VCPNCLQSIEE142 pKa = 4.38 RR143 pKa = 11.84 VRR145 pKa = 11.84 KK146 pKa = 8.43 MSASSACYY154 pKa = 9.68 HH155 pKa = 6.76 CCQNYY160 pKa = 10.52 NGGKK164 pKa = 7.36 YY165 pKa = 10.01 HH166 pKa = 7.05 SRR168 pKa = 11.84 FRR170 pKa = 11.84 LKK172 pKa = 10.74 AEE174 pKa = 4.9 RR175 pKa = 11.84 IAA177 pKa = 4.61
Molecular weight: 20.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.633
IPC_protein 10.292
Toseland 10.628
ProMoST 10.277
Dawson 10.73
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.643
DTASelect 10.409
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.701
IPC_peptide 10.833
IPC2_peptide 9.589
IPC2.peptide.svr19 8.562
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3805
0
3805
1291566
39
5242
339.4
37.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.554 ± 0.043
1.056 ± 0.014
5.861 ± 0.036
6.738 ± 0.048
3.876 ± 0.024
7.635 ± 0.052
2.281 ± 0.022
5.643 ± 0.028
5.661 ± 0.053
9.716 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.368 ± 0.022
3.766 ± 0.029
4.463 ± 0.027
3.638 ± 0.026
5.031 ± 0.038
6.83 ± 0.049
5.675 ± 0.047
6.614 ± 0.044
1.462 ± 0.016
3.132 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here