Atractylodes mild mottle virus
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M5K7P9|A0A0M5K7P9_9VIRU Protein 3 OS=Atractylodes mild mottle virus OX=1711685 PE=3 SV=1
MM1 pKa = 7.14 TNYY4 pKa = 8.5 MRR6 pKa = 11.84 NVEE9 pKa = 4.15 KK10 pKa = 10.66 FFYY13 pKa = 10.72 DD14 pKa = 3.18 KK15 pKa = 11.38 FEE17 pKa = 6.04 DD18 pKa = 3.98 DD19 pKa = 5.98 LEE21 pKa = 4.57 VTDD24 pKa = 4.44 EE25 pKa = 4.33 NYY27 pKa = 10.57 HH28 pKa = 5.98 ILNHH32 pKa = 5.8 TLMLMDD38 pKa = 5.28 MEE40 pKa = 5.79 NEE42 pKa = 3.97 DD43 pKa = 4.22 DD44 pKa = 3.99 ANSIIEE50 pKa = 4.51 NDD52 pKa = 3.62 PEE54 pKa = 4.15 LKK56 pKa = 10.55 KK57 pKa = 11.07 FFGHH61 pKa = 7.4 PDD63 pKa = 3.46 PEE65 pKa = 4.3 NLFVLGDD72 pKa = 3.88 EE73 pKa = 5.31 EE74 pKa = 4.54 VSEE77 pKa = 4.41 WNTEE81 pKa = 4.06 SEE83 pKa = 4.64 SEE85 pKa = 3.89 FDD87 pKa = 4.98 EE88 pKa = 5.88 LIQDD92 pKa = 3.61 EE93 pKa = 4.87 PPRR96 pKa = 11.84 KK97 pKa = 9.57 KK98 pKa = 9.66 PSEE101 pKa = 4.3 RR102 pKa = 11.84 IFPDD106 pKa = 2.94 QQMIDD111 pKa = 3.74 AYY113 pKa = 10.47 AQSSYY118 pKa = 10.69 TNPRR122 pKa = 11.84 THH124 pKa = 6.41 PTAYY128 pKa = 9.86 QDD130 pKa = 3.17 EE131 pKa = 5.01 AYY133 pKa = 10.53 SRR135 pKa = 11.84 RR136 pKa = 11.84 PNYY139 pKa = 9.97 YY140 pKa = 9.36 DD141 pKa = 3.68 SKK143 pKa = 10.61 DD144 pKa = 3.28 IPSFYY149 pKa = 10.64 DD150 pKa = 3.15 KK151 pKa = 10.82 KK152 pKa = 10.33 FKK154 pKa = 10.7 RR155 pKa = 11.84 SAFVGSMSGNMSGSVQTQFGLSLNIDD181 pKa = 3.84 CCGSAEE187 pKa = 4.28 RR188 pKa = 11.84 KK189 pKa = 9.4 IKK191 pKa = 10.44 LDD193 pKa = 3.08 QWLNEE198 pKa = 4.2 LNLMVQTNKK207 pKa = 10.39 EE208 pKa = 4.06 KK209 pKa = 10.66 FDD211 pKa = 3.7 TPKK214 pKa = 10.73 KK215 pKa = 9.9 VIILAEE221 pKa = 4.27 HH222 pKa = 7.01 KK223 pKa = 9.96 STGVLQNFIKK233 pKa = 9.95 RR234 pKa = 11.84 TTWNVDD240 pKa = 3.19 EE241 pKa = 5.41 FSTSQMADD249 pKa = 2.59 ILAEE253 pKa = 3.75 AVYY256 pKa = 11.15 ANFLGIDD263 pKa = 4.11 FRR265 pKa = 11.84 QSLVRR270 pKa = 11.84 EE271 pKa = 4.25 DD272 pKa = 3.34 EE273 pKa = 4.27 QIIKK277 pKa = 9.79 KK278 pKa = 10.46 AKK280 pKa = 9.3 EE281 pKa = 4.11 KK282 pKa = 9.11 MSKK285 pKa = 7.83 MTLCDD290 pKa = 2.93 ICFLDD295 pKa = 5.04 SFFCEE300 pKa = 4.4 YY301 pKa = 10.71 EE302 pKa = 4.33 SAFHH306 pKa = 6.44 SLKK309 pKa = 10.63 EE310 pKa = 3.71 RR311 pKa = 11.84 DD312 pKa = 3.97 DD313 pKa = 3.73 FVKK316 pKa = 10.9 FIEE319 pKa = 5.13 LYY321 pKa = 9.21 FAKK324 pKa = 10.31 IPIVGSKK331 pKa = 10.1 SLEE334 pKa = 3.9 RR335 pKa = 11.84 YY336 pKa = 9.41 KK337 pKa = 10.78 SEE339 pKa = 3.73 KK340 pKa = 9.8 TVVLEE345 pKa = 3.83 TSIAYY350 pKa = 10.0 ADD352 pKa = 4.43 RR353 pKa = 11.84 ITRR356 pKa = 11.84 EE357 pKa = 4.26 EE358 pKa = 3.72 ISKK361 pKa = 10.09 VCEE364 pKa = 3.69 FSKK367 pKa = 9.76 QQKK370 pKa = 9.37 RR371 pKa = 11.84 LKK373 pKa = 10.4 KK374 pKa = 10.35 FNKK377 pKa = 8.79 NCCAGLVEE385 pKa = 4.48 DD386 pKa = 4.24 QNLEE390 pKa = 4.24 FGCSISKK397 pKa = 10.46 SNRR400 pKa = 11.84 KK401 pKa = 9.21 KK402 pKa = 10.74 KK403 pKa = 10.74 SFSKK407 pKa = 10.41 KK408 pKa = 9.71 KK409 pKa = 9.3 KK410 pKa = 8.16 KK411 pKa = 10.05 KK412 pKa = 9.35 YY413 pKa = 9.25 YY414 pKa = 9.68 KK415 pKa = 9.97 KK416 pKa = 10.1 SYY418 pKa = 8.3 SKK420 pKa = 10.74 KK421 pKa = 8.5 RR422 pKa = 11.84 VRR424 pKa = 11.84 SKK426 pKa = 9.51 FQPRR430 pKa = 11.84 KK431 pKa = 9.42 FFRR434 pKa = 11.84 KK435 pKa = 9.58 KK436 pKa = 10.25 PPGKK440 pKa = 10.35 EE441 pKa = 3.74 KK442 pKa = 10.58 FCPKK446 pKa = 10.08 GKK448 pKa = 10.06 KK449 pKa = 8.7 SCRR452 pKa = 11.84 CWICSEE458 pKa = 3.87 QGHH461 pKa = 5.74 YY462 pKa = 11.22 ANEE465 pKa = 4.31 CPNRR469 pKa = 11.84 QAHH472 pKa = 4.91 QKK474 pKa = 9.28 QVNLFQEE481 pKa = 5.26 AIQRR485 pKa = 11.84 GLYY488 pKa = 9.55 PIEE491 pKa = 4.54 DD492 pKa = 4.25 PYY494 pKa = 11.36 EE495 pKa = 4.65 GEE497 pKa = 3.76 HH498 pKa = 6.13 HH499 pKa = 6.54 VYY501 pKa = 10.5 EE502 pKa = 5.21 FIVKK506 pKa = 9.91 EE507 pKa = 4.2 EE508 pKa = 4.27 PPDD511 pKa = 3.74 TDD513 pKa = 4.57 SEE515 pKa = 4.79 TTSSDD520 pKa = 3.02 GSSSEE525 pKa = 4.33 SSDD528 pKa = 3.85 SEE530 pKa = 4.22
Molecular weight: 61.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.803
IPC2_protein 5.893
IPC_protein 5.919
Toseland 6.109
ProMoST 6.135
Dawson 6.148
Bjellqvist 6.173
Wikipedia 6.135
Rodwell 6.122
Grimsley 6.186
Solomon 6.148
Lehninger 6.135
Nozaki 6.402
DTASelect 6.561
Thurlkill 6.561
EMBOSS 6.517
Sillero 6.504
Patrickios 4.279
IPC_peptide 6.16
IPC2_peptide 6.389
IPC2.peptide.svr19 6.535
Protein with the highest isoelectric point:
>tr|A0A0M4J5R0|A0A0M4J5R0_9VIRU Aphid transmission protein OS=Atractylodes mild mottle virus OX=1711685 PE=3 SV=1
MM1 pKa = 8.12 DD2 pKa = 6.41 LLQKK6 pKa = 10.51 VQIQNNHH13 pKa = 4.72 QQLSLNATNTSSIYY27 pKa = 10.28 ISGKK31 pKa = 10.55 LKK33 pKa = 10.18 FQGYY37 pKa = 8.64 KK38 pKa = 10.12 QLDD41 pKa = 3.48 LHH43 pKa = 6.69 CFVDD47 pKa = 4.6 TGASMCVASKK57 pKa = 10.62 HH58 pKa = 5.91 VIPEE62 pKa = 4.12 EE63 pKa = 3.6 HH64 pKa = 6.89 WEE66 pKa = 4.03 NSSRR70 pKa = 11.84 QLLVKK75 pKa = 8.86 TANGMITINKK85 pKa = 6.96 VCKK88 pKa = 10.63 NIDD91 pKa = 2.98 IMIADD96 pKa = 5.09 TIFHH100 pKa = 6.99 IPTIYY105 pKa = 9.76 QQEE108 pKa = 4.01 IGIDD112 pKa = 3.56 LLLGNNFLLLYY123 pKa = 10.84 GPFTQYY129 pKa = 9.33 TDD131 pKa = 4.16 RR132 pKa = 11.84 IILFKK137 pKa = 10.7 EE138 pKa = 3.8 NQPVIIGKK146 pKa = 8.56 VSKK149 pKa = 10.41 AYY151 pKa = 10.01 LHH153 pKa = 6.41 GMPGYY158 pKa = 10.15 LDD160 pKa = 3.69 SMKK163 pKa = 10.56 KK164 pKa = 10.2 NSRR167 pKa = 11.84 NPVPPPINITSNKK180 pKa = 9.5 IEE182 pKa = 4.17 DD183 pKa = 3.66 SKK185 pKa = 11.28 RR186 pKa = 11.84 FSVLKK191 pKa = 10.49 LQLLEE196 pKa = 4.16 RR197 pKa = 11.84 GRR199 pKa = 11.84 EE200 pKa = 3.93 IINRR204 pKa = 11.84 RR205 pKa = 11.84 LFLTQQDD212 pKa = 3.69 QLSTIEE218 pKa = 4.19 KK219 pKa = 10.38 LLDD222 pKa = 3.69 EE223 pKa = 4.86 VCSEE227 pKa = 4.56 NPIDD231 pKa = 3.6 PHH233 pKa = 8.2 KK234 pKa = 10.46 SKK236 pKa = 10.58 KK237 pKa = 9.29 WMTASIKK244 pKa = 10.88 LKK246 pKa = 10.75 DD247 pKa = 4.07 PNTLVQVKK255 pKa = 9.22 PMQYY259 pKa = 9.91 CPEE262 pKa = 4.08 DD263 pKa = 3.61 RR264 pKa = 11.84 KK265 pKa = 10.86 EE266 pKa = 3.7 FAVQIKK272 pKa = 9.94 EE273 pKa = 4.04 LLDD276 pKa = 3.66 LKK278 pKa = 10.75 IIIPSKK284 pKa = 10.12 SPHH287 pKa = 6.66 RR288 pKa = 11.84 SPAFLVEE295 pKa = 4.16 NEE297 pKa = 3.65 AEE299 pKa = 3.89 RR300 pKa = 11.84 RR301 pKa = 11.84 RR302 pKa = 11.84 GKK304 pKa = 10.54 KK305 pKa = 10.14 RR306 pKa = 11.84 MVVNYY311 pKa = 9.91 KK312 pKa = 10.22 KK313 pKa = 10.82 LNEE316 pKa = 4.03 VTLGDD321 pKa = 4.29 SHH323 pKa = 6.93 NLPNKK328 pKa = 10.49 DD329 pKa = 3.68 EE330 pKa = 4.84 LLTLIRR336 pKa = 11.84 GKK338 pKa = 9.05 TIYY341 pKa = 10.73 SSFDD345 pKa = 3.5 CKK347 pKa = 10.96 SGFWQVLLDD356 pKa = 4.39 QEE358 pKa = 4.77 SQLLTAFTCPTGHH371 pKa = 5.73 YY372 pKa = 7.52 QWKK375 pKa = 9.56 VVPFGLKK382 pKa = 8.89 QAPSIFQRR390 pKa = 11.84 HH391 pKa = 4.9 MNNAFRR397 pKa = 11.84 EE398 pKa = 4.29 FEE400 pKa = 4.33 EE401 pKa = 5.12 FCCVYY406 pKa = 10.75 VDD408 pKa = 5.62 DD409 pKa = 5.43 ILVFSKK415 pKa = 11.31 NEE417 pKa = 3.86 TEE419 pKa = 4.12 HH420 pKa = 6.43 RR421 pKa = 11.84 KK422 pKa = 9.31 HH423 pKa = 6.69 VIAILEE429 pKa = 4.02 QCKK432 pKa = 10.29 NLGIILSKK440 pKa = 10.75 KK441 pKa = 8.95 KK442 pKa = 10.55 AHH444 pKa = 6.62 LFKK447 pKa = 10.79 TKK449 pKa = 10.36 INFLGLEE456 pKa = 4.11 IDD458 pKa = 3.8 QGSHH462 pKa = 6.36 KK463 pKa = 9.39 PQNHH467 pKa = 5.81 ILEE470 pKa = 5.46 HH471 pKa = 5.17 IHH473 pKa = 6.66 KK474 pKa = 10.3 FPDD477 pKa = 3.12 RR478 pKa = 11.84 LEE480 pKa = 4.65 DD481 pKa = 3.39 KK482 pKa = 10.78 KK483 pKa = 11.01 QLQRR487 pKa = 11.84 FLGILTYY494 pKa = 10.95 ASDD497 pKa = 4.78 YY498 pKa = 10.58 IPKK501 pKa = 9.73 LAQIRR506 pKa = 11.84 KK507 pKa = 8.53 PFQAKK512 pKa = 9.74 LKK514 pKa = 10.76 KK515 pKa = 10.28 DD516 pKa = 3.96 VTWSWTDD523 pKa = 2.68 SDD525 pKa = 4.03 TSLMIKK531 pKa = 9.72 IKK533 pKa = 10.46 KK534 pKa = 8.69 GLKK537 pKa = 9.88 SFPTLYY543 pKa = 10.43 HH544 pKa = 6.54 PKK546 pKa = 10.55 EE547 pKa = 4.0 EE548 pKa = 4.67 DD549 pKa = 3.31 NLIIEE554 pKa = 5.12 CDD556 pKa = 3.45 ASDD559 pKa = 4.06 SFWGGILKK567 pKa = 10.6 AKK569 pKa = 9.84 TIDD572 pKa = 3.37 EE573 pKa = 4.42 DD574 pKa = 3.61 KK575 pKa = 11.01 EE576 pKa = 4.37 YY577 pKa = 10.45 ICRR580 pKa = 11.84 YY581 pKa = 7.54 TSGSFKK587 pKa = 10.4 QAEE590 pKa = 4.42 LNYY593 pKa = 10.1 HH594 pKa = 5.34 SNEE597 pKa = 3.92 KK598 pKa = 10.48 EE599 pKa = 3.42 ILAAMNTIKK608 pKa = 10.64 KK609 pKa = 9.93 FSGYY613 pKa = 7.92 LTPVKK618 pKa = 10.31 FLIRR622 pKa = 11.84 TDD624 pKa = 3.25 NKK626 pKa = 10.66 NFTFFLNTNHH636 pKa = 7.12 KK637 pKa = 10.45 GDD639 pKa = 3.9 YY640 pKa = 9.4 KK641 pKa = 10.28 QGRR644 pKa = 11.84 LIRR647 pKa = 11.84 WQMWFSRR654 pKa = 11.84 YY655 pKa = 9.88 SFTVEE660 pKa = 4.0 HH661 pKa = 6.67 LPGNKK666 pKa = 9.3 NVFADD671 pKa = 4.28 FLTRR675 pKa = 11.84 EE676 pKa = 4.33 FNNN679 pKa = 3.45
Molecular weight: 78.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.653
IPC2_protein 8.507
IPC_protein 8.346
Toseland 9.18
ProMoST 8.96
Dawson 9.458
Bjellqvist 9.238
Wikipedia 9.589
Rodwell 9.809
Grimsley 9.487
Solomon 9.487
Lehninger 9.458
Nozaki 9.443
DTASelect 9.151
Thurlkill 9.341
EMBOSS 9.633
Sillero 9.472
Patrickios 4.978
IPC_peptide 9.487
IPC2_peptide 7.805
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2358
120
679
393.0
45.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.411 ± 0.455
1.739 ± 0.238
5.895 ± 0.356
7.591 ± 0.741
5.047 ± 0.408
4.241 ± 0.248
2.417 ± 0.342
6.955 ± 0.539
11.323 ± 0.62
8.567 ± 0.78
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.696 ± 0.212
5.768 ± 0.579
4.368 ± 0.5
4.283 ± 0.309
3.562 ± 0.332
8.482 ± 0.649
4.665 ± 0.235
5.089 ± 0.477
0.721 ± 0.187
3.181 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here