Thielaviopsis basicola mitovirus
Average proteome isoelectric point is 8.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6Q2W5|Q6Q2W5_9VIRU RNA-dependent RNA polymerase OS=Thielaviopsis basicola mitovirus OX=274584 PE=4 SV=1
MM1 pKa = 7.44 IKK3 pKa = 10.43 YY4 pKa = 7.0 MKK6 pKa = 9.95 SVRR9 pKa = 11.84 LHH11 pKa = 4.55 ITRR14 pKa = 11.84 YY15 pKa = 8.93 ICRR18 pKa = 11.84 KK19 pKa = 8.83 PLFINDD25 pKa = 3.55 SGVSVDD31 pKa = 4.31 KK32 pKa = 11.37 SGFPTKK38 pKa = 10.53 FIEE41 pKa = 5.17 LKK43 pKa = 10.67 KK44 pKa = 10.48 FLNNHH49 pKa = 5.82 EE50 pKa = 4.41 SSICYY55 pKa = 7.68 HH56 pKa = 5.15 TRR58 pKa = 11.84 LRR60 pKa = 11.84 IILTLLLLPRR70 pKa = 11.84 GLKK73 pKa = 7.37 PTKK76 pKa = 10.27 IEE78 pKa = 4.01 DD79 pKa = 3.55 AKK81 pKa = 10.72 IKK83 pKa = 10.58 PNFSSITLPFSGTRR97 pKa = 11.84 MNPIPTWFIKK107 pKa = 10.62 EE108 pKa = 3.88 FVKK111 pKa = 10.65 EE112 pKa = 3.97 YY113 pKa = 10.64 NLKK116 pKa = 10.58 LEE118 pKa = 4.4 KK119 pKa = 9.22 PTYY122 pKa = 10.12 DD123 pKa = 3.2 LSKK126 pKa = 10.58 HH127 pKa = 4.92 YY128 pKa = 10.12 LSPKK132 pKa = 8.55 GGPMGKK138 pKa = 8.08 STWSSIWSMYY148 pKa = 9.59 ALKK151 pKa = 10.72 HH152 pKa = 5.42 EE153 pKa = 4.41 LHH155 pKa = 6.61 LSLSYY160 pKa = 10.78 FLGEE164 pKa = 3.9 AYY166 pKa = 7.57 KK167 pKa = 10.11 TLFYY171 pKa = 11.01 LPYY174 pKa = 9.15 LKK176 pKa = 10.86 NMALSYY182 pKa = 10.94 GHH184 pKa = 7.23 DD185 pKa = 3.9 VPPKK189 pKa = 8.34 QWPSGKK195 pKa = 10.26 LGIVKK200 pKa = 10.26 DD201 pKa = 4.0 PEE203 pKa = 4.01 GKK205 pKa = 9.82 RR206 pKa = 11.84 RR207 pKa = 11.84 IIAMVDD213 pKa = 3.06 YY214 pKa = 10.6 HH215 pKa = 6.58 SQLVLRR221 pKa = 11.84 SIHH224 pKa = 7.51 DD225 pKa = 3.56 GLLNKK230 pKa = 9.71 LRR232 pKa = 11.84 NLPQDD237 pKa = 2.95 RR238 pKa = 11.84 TYY240 pKa = 11.55 NQDD243 pKa = 3.38 PNNAWEE249 pKa = 4.04 EE250 pKa = 4.07 NKK252 pKa = 10.54 EE253 pKa = 4.24 CFHH256 pKa = 8.14 SLDD259 pKa = 4.13 LSSATDD265 pKa = 3.76 RR266 pKa = 11.84 FPVKK270 pKa = 10.18 LQSRR274 pKa = 11.84 LLTEE278 pKa = 4.7 MYY280 pKa = 10.32 SDD282 pKa = 3.98 PSFGEE287 pKa = 3.26 NWMNLLLNRR296 pKa = 11.84 DD297 pKa = 3.63 YY298 pKa = 11.49 LLPEE302 pKa = 4.46 EE303 pKa = 4.59 GLSGEE308 pKa = 4.16 RR309 pKa = 11.84 LRR311 pKa = 11.84 YY312 pKa = 9.52 AVGQPMGAYY321 pKa = 9.89 SSWAAFTLSHH331 pKa = 6.52 HH332 pKa = 6.89 LVVAWCTYY340 pKa = 9.64 KK341 pKa = 10.44 SKK343 pKa = 11.02 KK344 pKa = 9.8 VIRR347 pKa = 11.84 SSQYY351 pKa = 10.25 IILGDD356 pKa = 4.4 DD357 pKa = 3.66 IVIKK361 pKa = 11.03 DD362 pKa = 3.33 NDD364 pKa = 3.4 IARR367 pKa = 11.84 KK368 pKa = 10.13 YY369 pKa = 9.03 IGQMSKK375 pKa = 10.84 LGVAISMQKK384 pKa = 7.76 THH386 pKa = 5.51 VSKK389 pKa = 10.08 DD390 pKa = 3.06 TYY392 pKa = 10.88 EE393 pKa = 3.94 FAKK396 pKa = 10.15 RR397 pKa = 11.84 WMHH400 pKa = 6.36 KK401 pKa = 9.87 GVEE404 pKa = 4.09 ISGLPLKK411 pKa = 10.87 GIFYY415 pKa = 10.02 NISHH419 pKa = 6.48 MRR421 pKa = 11.84 RR422 pKa = 11.84 MYY424 pKa = 10.28 TIIFDD429 pKa = 3.78 YY430 pKa = 10.47 LQRR433 pKa = 11.84 IPSKK437 pKa = 11.32 SNLTSLQLFASCLSGIKK454 pKa = 9.8 FGKK457 pKa = 9.6 RR458 pKa = 11.84 VYY460 pKa = 10.88 SKK462 pKa = 11.56 ALILKK467 pKa = 9.19 LLRR470 pKa = 11.84 NFNISLRR477 pKa = 11.84 YY478 pKa = 9.65 SYY480 pKa = 11.56 GLLTPYY486 pKa = 10.41 EE487 pKa = 4.09 LRR489 pKa = 11.84 EE490 pKa = 4.06 YY491 pKa = 10.56 ILTGFNMGDD500 pKa = 3.19 EE501 pKa = 4.58 VLPSDD506 pKa = 4.63 NRR508 pKa = 11.84 ILEE511 pKa = 4.13 WFKK514 pKa = 11.4 GIICDD519 pKa = 3.86 GVAGKK524 pKa = 10.74 VSTLSRR530 pKa = 11.84 EE531 pKa = 3.89 YY532 pKa = 9.73 TKK534 pKa = 10.64 EE535 pKa = 3.99 VNSLEE540 pKa = 4.16 EE541 pKa = 4.22 HH542 pKa = 6.85 RR543 pKa = 11.84 SSQHH547 pKa = 5.33 ILNSINIEE555 pKa = 3.63 ITYY558 pKa = 10.73 EE559 pKa = 3.89 NLQWSPLIIGMFNHH573 pKa = 6.38 INKK576 pKa = 9.19 VSEE579 pKa = 4.31 KK580 pKa = 10.23 CGEE583 pKa = 3.88 WLYY586 pKa = 11.67 GDD588 pKa = 4.34 VPLMDD593 pKa = 5.88 IIQQFTLPSADD604 pKa = 3.27 SLSRR608 pKa = 11.84 KK609 pKa = 9.92 DD610 pKa = 3.48 RR611 pKa = 11.84 DD612 pKa = 3.35 INKK615 pKa = 10.27 KK616 pKa = 9.59 IDD618 pKa = 3.71 EE619 pKa = 4.67 LDD621 pKa = 3.44 SLIFKK626 pKa = 9.95 SIKK629 pKa = 9.9 RR630 pKa = 11.84 HH631 pKa = 6.22 FSDD634 pKa = 4.3 QPYY637 pKa = 9.32 PDD639 pKa = 3.92 HH640 pKa = 7.65 FYY642 pKa = 10.09 GTRR645 pKa = 11.84 SNGDD649 pKa = 3.06 KK650 pKa = 10.77 NLYY653 pKa = 9.05 PGLGALDD660 pKa = 3.61 VNVAPEE666 pKa = 3.64 IGFEE670 pKa = 3.95 IKK672 pKa = 10.76 SPINFILKK680 pKa = 8.86 TEE682 pKa = 4.0 IQRR685 pKa = 11.84 LHH687 pKa = 6.74 GNLEE691 pKa = 3.99 MQMEE695 pKa = 4.43 KK696 pKa = 10.42 PLRR699 pKa = 11.84 EE700 pKa = 4.08 AGIKK704 pKa = 9.87 CC705 pKa = 3.76
Molecular weight: 81.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.727
IPC2_protein 8.726
IPC_protein 8.595
Toseland 9.238
ProMoST 9.092
Dawson 9.56
Bjellqvist 9.326
Wikipedia 9.75
Rodwell 9.794
Grimsley 9.648
Solomon 9.589
Lehninger 9.531
Nozaki 9.37
DTASelect 9.282
Thurlkill 9.399
EMBOSS 9.692
Sillero 9.516
Patrickios 4.596
IPC_peptide 9.575
IPC2_peptide 7.907
IPC2.peptide.svr19 7.748
Protein with the highest isoelectric point:
>tr|Q6Q2W5|Q6Q2W5_9VIRU RNA-dependent RNA polymerase OS=Thielaviopsis basicola mitovirus OX=274584 PE=4 SV=1
MM1 pKa = 7.44 IKK3 pKa = 10.43 YY4 pKa = 7.0 MKK6 pKa = 9.95 SVRR9 pKa = 11.84 LHH11 pKa = 4.55 ITRR14 pKa = 11.84 YY15 pKa = 8.93 ICRR18 pKa = 11.84 KK19 pKa = 8.83 PLFINDD25 pKa = 3.55 SGVSVDD31 pKa = 4.31 KK32 pKa = 11.37 SGFPTKK38 pKa = 10.53 FIEE41 pKa = 5.17 LKK43 pKa = 10.67 KK44 pKa = 10.48 FLNNHH49 pKa = 5.82 EE50 pKa = 4.41 SSICYY55 pKa = 7.68 HH56 pKa = 5.15 TRR58 pKa = 11.84 LRR60 pKa = 11.84 IILTLLLLPRR70 pKa = 11.84 GLKK73 pKa = 7.37 PTKK76 pKa = 10.27 IEE78 pKa = 4.01 DD79 pKa = 3.55 AKK81 pKa = 10.72 IKK83 pKa = 10.58 PNFSSITLPFSGTRR97 pKa = 11.84 MNPIPTWFIKK107 pKa = 10.62 EE108 pKa = 3.88 FVKK111 pKa = 10.65 EE112 pKa = 3.97 YY113 pKa = 10.64 NLKK116 pKa = 10.58 LEE118 pKa = 4.4 KK119 pKa = 9.22 PTYY122 pKa = 10.12 DD123 pKa = 3.2 LSKK126 pKa = 10.58 HH127 pKa = 4.92 YY128 pKa = 10.12 LSPKK132 pKa = 8.55 GGPMGKK138 pKa = 8.08 STWSSIWSMYY148 pKa = 9.59 ALKK151 pKa = 10.72 HH152 pKa = 5.42 EE153 pKa = 4.41 LHH155 pKa = 6.61 LSLSYY160 pKa = 10.78 FLGEE164 pKa = 3.9 AYY166 pKa = 7.57 KK167 pKa = 10.11 TLFYY171 pKa = 11.01 LPYY174 pKa = 9.15 LKK176 pKa = 10.86 NMALSYY182 pKa = 10.94 GHH184 pKa = 7.23 DD185 pKa = 3.9 VPPKK189 pKa = 8.34 QWPSGKK195 pKa = 10.26 LGIVKK200 pKa = 10.26 DD201 pKa = 4.0 PEE203 pKa = 4.01 GKK205 pKa = 9.82 RR206 pKa = 11.84 RR207 pKa = 11.84 IIAMVDD213 pKa = 3.06 YY214 pKa = 10.6 HH215 pKa = 6.58 SQLVLRR221 pKa = 11.84 SIHH224 pKa = 7.51 DD225 pKa = 3.56 GLLNKK230 pKa = 9.71 LRR232 pKa = 11.84 NLPQDD237 pKa = 2.95 RR238 pKa = 11.84 TYY240 pKa = 11.55 NQDD243 pKa = 3.38 PNNAWEE249 pKa = 4.04 EE250 pKa = 4.07 NKK252 pKa = 10.54 EE253 pKa = 4.24 CFHH256 pKa = 8.14 SLDD259 pKa = 4.13 LSSATDD265 pKa = 3.76 RR266 pKa = 11.84 FPVKK270 pKa = 10.18 LQSRR274 pKa = 11.84 LLTEE278 pKa = 4.7 MYY280 pKa = 10.32 SDD282 pKa = 3.98 PSFGEE287 pKa = 3.26 NWMNLLLNRR296 pKa = 11.84 DD297 pKa = 3.63 YY298 pKa = 11.49 LLPEE302 pKa = 4.46 EE303 pKa = 4.59 GLSGEE308 pKa = 4.16 RR309 pKa = 11.84 LRR311 pKa = 11.84 YY312 pKa = 9.52 AVGQPMGAYY321 pKa = 9.89 SSWAAFTLSHH331 pKa = 6.52 HH332 pKa = 6.89 LVVAWCTYY340 pKa = 9.64 KK341 pKa = 10.44 SKK343 pKa = 11.02 KK344 pKa = 9.8 VIRR347 pKa = 11.84 SSQYY351 pKa = 10.25 IILGDD356 pKa = 4.4 DD357 pKa = 3.66 IVIKK361 pKa = 11.03 DD362 pKa = 3.33 NDD364 pKa = 3.4 IARR367 pKa = 11.84 KK368 pKa = 10.13 YY369 pKa = 9.03 IGQMSKK375 pKa = 10.84 LGVAISMQKK384 pKa = 7.76 THH386 pKa = 5.51 VSKK389 pKa = 10.08 DD390 pKa = 3.06 TYY392 pKa = 10.88 EE393 pKa = 3.94 FAKK396 pKa = 10.15 RR397 pKa = 11.84 WMHH400 pKa = 6.36 KK401 pKa = 9.87 GVEE404 pKa = 4.09 ISGLPLKK411 pKa = 10.87 GIFYY415 pKa = 10.02 NISHH419 pKa = 6.48 MRR421 pKa = 11.84 RR422 pKa = 11.84 MYY424 pKa = 10.28 TIIFDD429 pKa = 3.78 YY430 pKa = 10.47 LQRR433 pKa = 11.84 IPSKK437 pKa = 11.32 SNLTSLQLFASCLSGIKK454 pKa = 9.8 FGKK457 pKa = 9.6 RR458 pKa = 11.84 VYY460 pKa = 10.88 SKK462 pKa = 11.56 ALILKK467 pKa = 9.19 LLRR470 pKa = 11.84 NFNISLRR477 pKa = 11.84 YY478 pKa = 9.65 SYY480 pKa = 11.56 GLLTPYY486 pKa = 10.41 EE487 pKa = 4.09 LRR489 pKa = 11.84 EE490 pKa = 4.06 YY491 pKa = 10.56 ILTGFNMGDD500 pKa = 3.19 EE501 pKa = 4.58 VLPSDD506 pKa = 4.63 NRR508 pKa = 11.84 ILEE511 pKa = 4.13 WFKK514 pKa = 11.4 GIICDD519 pKa = 3.86 GVAGKK524 pKa = 10.74 VSTLSRR530 pKa = 11.84 EE531 pKa = 3.89 YY532 pKa = 9.73 TKK534 pKa = 10.64 EE535 pKa = 3.99 VNSLEE540 pKa = 4.16 EE541 pKa = 4.22 HH542 pKa = 6.85 RR543 pKa = 11.84 SSQHH547 pKa = 5.33 ILNSINIEE555 pKa = 3.63 ITYY558 pKa = 10.73 EE559 pKa = 3.89 NLQWSPLIIGMFNHH573 pKa = 6.38 INKK576 pKa = 9.19 VSEE579 pKa = 4.31 KK580 pKa = 10.23 CGEE583 pKa = 3.88 WLYY586 pKa = 11.67 GDD588 pKa = 4.34 VPLMDD593 pKa = 5.88 IIQQFTLPSADD604 pKa = 3.27 SLSRR608 pKa = 11.84 KK609 pKa = 9.92 DD610 pKa = 3.48 RR611 pKa = 11.84 DD612 pKa = 3.35 INKK615 pKa = 10.27 KK616 pKa = 9.59 IDD618 pKa = 3.71 EE619 pKa = 4.67 LDD621 pKa = 3.44 SLIFKK626 pKa = 9.95 SIKK629 pKa = 9.9 RR630 pKa = 11.84 HH631 pKa = 6.22 FSDD634 pKa = 4.3 QPYY637 pKa = 9.32 PDD639 pKa = 3.92 HH640 pKa = 7.65 FYY642 pKa = 10.09 GTRR645 pKa = 11.84 SNGDD649 pKa = 3.06 KK650 pKa = 10.77 NLYY653 pKa = 9.05 PGLGALDD660 pKa = 3.61 VNVAPEE666 pKa = 3.64 IGFEE670 pKa = 3.95 IKK672 pKa = 10.76 SPINFILKK680 pKa = 8.86 TEE682 pKa = 4.0 IQRR685 pKa = 11.84 LHH687 pKa = 6.74 GNLEE691 pKa = 3.99 MQMEE695 pKa = 4.43 KK696 pKa = 10.42 PLRR699 pKa = 11.84 EE700 pKa = 4.08 AGIKK704 pKa = 9.87 CC705 pKa = 3.76
Molecular weight: 81.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.727
IPC2_protein 8.726
IPC_protein 8.595
Toseland 9.238
ProMoST 9.092
Dawson 9.56
Bjellqvist 9.326
Wikipedia 9.75
Rodwell 9.794
Grimsley 9.648
Solomon 9.589
Lehninger 9.531
Nozaki 9.37
DTASelect 9.282
Thurlkill 9.399
EMBOSS 9.692
Sillero 9.516
Patrickios 4.596
IPC_peptide 9.575
IPC2_peptide 7.907
IPC2.peptide.svr19 7.748
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
705
705
705
705.0
81.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.121 ± 0.0
1.135 ± 0.0
4.823 ± 0.0
5.39 ± 0.0
4.113 ± 0.0
5.957 ± 0.0
2.979 ± 0.0
8.227 ± 0.0
8.369 ± 0.0
11.915 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.837 ± 0.0
4.965 ± 0.0
4.965 ± 0.0
2.553 ± 0.0
5.106 ± 0.0
9.078 ± 0.0
3.972 ± 0.0
3.688 ± 0.0
1.702 ± 0.0
5.106 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here