Narcissus common latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0VZD1|Q0VZD1_9VIRU Helicase OS=Narcissus common latent virus OX=160844 GN=NcLaVgp1 PE=3 SV=1
MM1 pKa = 7.82DD2 pKa = 3.99VLVNLLNKK10 pKa = 10.09YY11 pKa = 10.15GLVRR15 pKa = 11.84LSSKK19 pKa = 10.45LVLPIVVHH27 pKa = 6.02CVPGAGKK34 pKa = 8.46STLIRR39 pKa = 11.84EE40 pKa = 4.57LLACDD45 pKa = 3.72SRR47 pKa = 11.84FVAYY51 pKa = 9.28TGGLGDD57 pKa = 4.14PAHH60 pKa = 6.68ITGKK64 pKa = 9.5WIQRR68 pKa = 11.84WQGDD72 pKa = 3.38YY73 pKa = 11.42DD74 pKa = 3.6RR75 pKa = 11.84TKK77 pKa = 11.25NLVLDD82 pKa = 4.97EE83 pKa = 4.3YY84 pKa = 10.94TLIEE88 pKa = 4.5DD89 pKa = 3.77VPGAFALFGDD99 pKa = 5.27PIQVNTTSVKK109 pKa = 9.87PADD112 pKa = 4.65FICYY116 pKa = 6.72TSRR119 pKa = 11.84RR120 pKa = 11.84FGSATSTLLKK130 pKa = 10.74ALGWPVEE137 pKa = 3.97ASGNDD142 pKa = 3.47LVQIHH147 pKa = 7.1HH148 pKa = 7.14IYY150 pKa = 10.48SFEE153 pKa = 3.9PTGVVIYY160 pKa = 9.99FEE162 pKa = 4.76EE163 pKa = 4.96EE164 pKa = 3.56IGCLLRR170 pKa = 11.84EE171 pKa = 4.25HH172 pKa = 6.73CVAALGLEE180 pKa = 4.42EE181 pKa = 4.7IRR183 pKa = 11.84GKK185 pKa = 8.75TFDD188 pKa = 3.18TVTFVTSEE196 pKa = 3.87NSPLISRR203 pKa = 11.84EE204 pKa = 3.64AAYY207 pKa = 10.35QCLTRR212 pKa = 11.84HH213 pKa = 6.02RR214 pKa = 11.84LALHH218 pKa = 6.31ILCPNATYY226 pKa = 7.8TAPP229 pKa = 3.55

Molecular weight: 25.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0VZD0|Q0VZD0_9VIRU Triple Gene Block protein 1 OS=Narcissus common latent virus OX=160844 GN=NcLaVgp2 PE=4 SV=1
MM1 pKa = 7.93PLTPPRR7 pKa = 11.84DD8 pKa = 3.58FTKK11 pKa = 10.71VYY13 pKa = 9.9IAASVGATLALVTWLLTKK31 pKa = 9.83NTLPAVGDD39 pKa = 3.64RR40 pKa = 11.84DD41 pKa = 3.62HH42 pKa = 6.88NLPHH46 pKa = 6.8GGLYY50 pKa = 9.99RR51 pKa = 11.84DD52 pKa = 3.49GTKK55 pKa = 10.23AIKK58 pKa = 10.5YY59 pKa = 7.91NSPCKK64 pKa = 10.18LNSIEE69 pKa = 4.06GHH71 pKa = 6.24SRR73 pKa = 11.84GIWNQPWALVFILSVLIIISNRR95 pKa = 11.84LDD97 pKa = 3.18SRR99 pKa = 11.84VCRR102 pKa = 11.84RR103 pKa = 11.84CGTTHH108 pKa = 7.2

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2767

61

1966

461.2

51.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.987 ± 0.768

2.783 ± 0.421

4.445 ± 0.593

5.891 ± 0.741

5.204 ± 0.825

5.819 ± 0.611

2.927 ± 0.488

4.843 ± 0.445

5.313 ± 0.838

10.481 ± 0.782

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.287

4.192 ± 0.473

4.662 ± 0.341

3.144 ± 0.425

6.108 ± 0.702

7.264 ± 0.28

5.891 ± 0.789

6.397 ± 0.738

1.265 ± 0.15

3.506 ± 0.487
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski