Streptococcus phage Javan74

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BIF1|A0A4D6BIF1_9CAUD Integrase OS=Streptococcus phage Javan74 OX=2548305 GN=Javan74_0052 PE=3 SV=1
MM1 pKa = 7.34QSTGLKK7 pKa = 10.09DD8 pKa = 3.56KK9 pKa = 10.8NGKK12 pKa = 9.57EE13 pKa = 3.83IFEE16 pKa = 4.32GDD18 pKa = 3.55IIATNSFACIVSFGKK33 pKa = 10.46YY34 pKa = 10.05DD35 pKa = 3.43YY36 pKa = 11.48FEE38 pKa = 6.18DD39 pKa = 4.82DD40 pKa = 4.27GIKK43 pKa = 8.73ITGIGFYY50 pKa = 10.39LGYY53 pKa = 10.77LNVEE57 pKa = 4.17PATYY61 pKa = 10.61SPFEE65 pKa = 4.22SPFLEE70 pKa = 4.32NCEE73 pKa = 3.99VLGNIHH79 pKa = 6.91EE80 pKa = 4.46NMLDD84 pKa = 3.81LIMYY88 pKa = 7.32EE89 pKa = 3.93KK90 pKa = 9.97WKK92 pKa = 10.59SEE94 pKa = 3.28NWEE97 pKa = 3.95LMEE100 pKa = 5.15GNVV103 pKa = 3.83

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BDC7|A0A4D6BDC7_9CAUD Site-specific DNA-methyltransferase (adenine-specific) OS=Streptococcus phage Javan74 OX=2548305 GN=Javan74_0039 PE=3 SV=1
MM1 pKa = 8.05IEE3 pKa = 4.53FFLPMKK9 pKa = 10.26KK10 pKa = 9.78IPTTTHH16 pKa = 3.97QQKK19 pKa = 10.75KK20 pKa = 7.35ITVKK24 pKa = 10.34NSKK27 pKa = 8.71PQIYY31 pKa = 9.89EE32 pKa = 3.98PEE34 pKa = 4.1SLKK37 pKa = 10.8AARR40 pKa = 11.84EE41 pKa = 4.09KK42 pKa = 10.76FLSLLAQHH50 pKa = 6.42VPPNKK55 pKa = 9.62FKK57 pKa = 10.85GAIRR61 pKa = 11.84LTVKK65 pKa = 9.36WCFPRR70 pKa = 11.84IKK72 pKa = 10.12KK73 pKa = 10.66SYY75 pKa = 9.34DD76 pKa = 3.12GQYY79 pKa = 9.06KK80 pKa = 6.44TTKK83 pKa = 9.9PDD85 pKa = 3.23TDD87 pKa = 3.57NLQKK91 pKa = 10.91LLKK94 pKa = 10.67DD95 pKa = 3.78CMTEE99 pKa = 3.99LKK101 pKa = 10.1YY102 pKa = 10.57WKK104 pKa = 10.01DD105 pKa = 3.44DD106 pKa = 3.69ALVASEE112 pKa = 4.12IVEE115 pKa = 4.38KK116 pKa = 10.54FWADD120 pKa = 3.22TVGIYY125 pKa = 10.05IRR127 pKa = 11.84IEE129 pKa = 3.77EE130 pKa = 4.47LEE132 pKa = 3.96

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11937

37

1208

229.6

26.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.188 ± 0.367

0.528 ± 0.098

6.409 ± 0.255

7.967 ± 0.471

3.946 ± 0.246

6.333 ± 0.412

1.483 ± 0.149

6.492 ± 0.285

8.118 ± 0.244

8.528 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.151

5.47 ± 0.231

3.091 ± 0.222

4.515 ± 0.294

4.633 ± 0.343

5.881 ± 0.251

5.872 ± 0.381

6.115 ± 0.241

1.273 ± 0.11

3.971 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski