Vibrio phage VAI1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D0NNU6|A0A7D0NNU6_9VIRU RstR-like putative transcriptional regulator OS=Vibrio phage VAI1 OX=2601671 GN=VAI1_11 PE=4 SV=1
MM1 pKa = 7.67LEE3 pKa = 4.32LVSDD7 pKa = 4.63VITVLIALSTGGAFVYY23 pKa = 10.16GVYY26 pKa = 9.99TGINASS32 pKa = 3.37

Molecular weight: 3.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D0JHG5|A0A7D0JHG5_9VIRU Uncharacterized protein OS=Vibrio phage VAI1 OX=2601671 GN=VAI1_10 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.92YY3 pKa = 10.62EE4 pKa = 3.99HH5 pKa = 7.2ALCEE9 pKa = 4.2LCVRR13 pKa = 11.84ASLANRR19 pKa = 11.84FSFLACTQQAATMSKK34 pKa = 10.26SIAHH38 pKa = 5.9SLPRR42 pKa = 11.84SGQRR46 pKa = 11.84QTLNIIHH53 pKa = 6.2NHH55 pKa = 4.19NTHH58 pKa = 6.36FKK60 pKa = 10.32KK61 pKa = 10.75

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

1851

32

454

168.3

18.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.672 ± 0.578

1.675 ± 0.255

5.835 ± 0.592

4.268 ± 0.538

5.186 ± 0.91

7.185 ± 0.66

1.891 ± 0.531

6.699 ± 0.668

5.727 ± 0.656

9.833 ± 0.537

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.385

4.97 ± 1.017

3.944 ± 0.608

3.512 ± 0.256

4.484 ± 0.85

8.536 ± 0.725

5.781 ± 0.623

5.727 ± 0.824

0.972 ± 0.323

3.89 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski