Microviridae phi-CA82

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8RW83|F8RW83_9VIRU Structural protein VP2 OS=Microviridae phi-CA82 OX=913970 GN=VP2 PE=4 SV=1
MM1 pKa = 7.61HH2 pKa = 7.08MRR4 pKa = 11.84PGFNAGLCIPIYY16 pKa = 10.32IEE18 pKa = 4.75EE19 pKa = 4.49ILPNDD24 pKa = 3.84TVEE27 pKa = 4.46MNVNMALRR35 pKa = 11.84MQTLATVPYY44 pKa = 10.6DD45 pKa = 4.12NLDD48 pKa = 3.6LDD50 pKa = 5.57LYY52 pKa = 10.26WFYY55 pKa = 11.69APNRR59 pKa = 11.84ILWDD63 pKa = 3.28GWKK66 pKa = 9.79QFCGEE71 pKa = 4.65SKK73 pKa = 10.71SAWLDD78 pKa = 3.33KK79 pKa = 10.92TPLRR83 pKa = 11.84VPVLKK88 pKa = 10.12TSGFANPDD96 pKa = 3.6DD97 pKa = 5.34GIGTVWDD104 pKa = 4.0YY105 pKa = 11.72FGLPLIKK112 pKa = 9.85EE113 pKa = 4.54TTDD116 pKa = 2.72KK117 pKa = 10.92SKK119 pKa = 10.95RR120 pKa = 11.84IAINRR125 pKa = 11.84LPFNMYY131 pKa = 10.67AKK133 pKa = 10.01VWNDD137 pKa = 2.66WFRR140 pKa = 11.84DD141 pKa = 3.46TNYY144 pKa = 10.25QEE146 pKa = 4.34EE147 pKa = 4.07ALIDD151 pKa = 3.78YY152 pKa = 9.94GNTDD156 pKa = 2.97VTYY159 pKa = 10.76DD160 pKa = 3.53KK161 pKa = 11.23SDD163 pKa = 3.5PAKK166 pKa = 10.53GGKK169 pKa = 8.55PLSANKK175 pKa = 9.5YY176 pKa = 7.72HH177 pKa = 8.07DD178 pKa = 4.3YY179 pKa = 9.58FTTCLPSPQRR189 pKa = 11.84GEE191 pKa = 4.31SVSLLDD197 pKa = 4.6GIRR200 pKa = 11.84NSVLPVRR207 pKa = 11.84AVGTEE212 pKa = 3.9LEE214 pKa = 4.44GDD216 pKa = 3.75NEE218 pKa = 5.11LGILWGLYY226 pKa = 9.7NKK228 pKa = 10.29NNEE231 pKa = 4.09QNFLPDD237 pKa = 2.59AWKK240 pKa = 9.59WYY242 pKa = 10.34DD243 pKa = 3.21MTYY246 pKa = 10.52QADD249 pKa = 3.49NSAFLHH255 pKa = 5.49QYY257 pKa = 8.46YY258 pKa = 10.58NKK260 pKa = 10.73ASGTSTAPTDD270 pKa = 3.62KK271 pKa = 10.97ATMTPANLGVDD282 pKa = 3.39IRR284 pKa = 11.84GADD287 pKa = 3.57YY288 pKa = 11.23NSNKK292 pKa = 10.24SFVDD296 pKa = 3.39AMNLTEE302 pKa = 4.36LRR304 pKa = 11.84KK305 pKa = 10.31SIAKK309 pKa = 7.86QHH311 pKa = 5.7IAEE314 pKa = 4.14VSARR318 pKa = 11.84CGNRR322 pKa = 11.84YY323 pKa = 9.87EE324 pKa = 4.2NFLLGFFGVSAPDD337 pKa = 3.24IEE339 pKa = 4.52TGRR342 pKa = 11.84TEE344 pKa = 4.15LLGINHH350 pKa = 5.56MTLNISEE357 pKa = 4.53VVQTSASEE365 pKa = 4.17EE366 pKa = 4.25GSTPQGNISGRR377 pKa = 11.84SLTNMDD383 pKa = 3.9NPHH386 pKa = 7.03AFTKK390 pKa = 10.73SFTEE394 pKa = 4.61HH395 pKa = 5.27GWLMCIAVARR405 pKa = 11.84YY406 pKa = 7.97NHH408 pKa = 6.69SYY410 pKa = 9.83SQGIEE415 pKa = 4.28KK416 pKa = 10.26KK417 pKa = 4.3WTRR420 pKa = 11.84YY421 pKa = 9.86KK422 pKa = 11.04LEE424 pKa = 4.5DD425 pKa = 3.81YY426 pKa = 11.41YY427 pKa = 11.07MPEE430 pKa = 4.16YY431 pKa = 11.22ANMGDD436 pKa = 3.54VPVYY440 pKa = 10.44LSEE443 pKa = 5.0IYY445 pKa = 9.18ATNANAQNPDD455 pKa = 3.59NLTGSDD461 pKa = 3.39GKK463 pKa = 10.56NPQEE467 pKa = 5.29DD468 pKa = 2.65IWGYY472 pKa = 7.66QEE474 pKa = 4.68YY475 pKa = 9.78GAEE478 pKa = 4.14YY479 pKa = 9.79KK480 pKa = 10.81YY481 pKa = 11.21SPNKK485 pKa = 8.42VTGSLRR491 pKa = 11.84PDD493 pKa = 2.84IKK495 pKa = 10.99GGLSPWTWCDD505 pKa = 3.3NYY507 pKa = 11.23DD508 pKa = 4.24SEE510 pKa = 4.72PTNGADD516 pKa = 4.74WIKK519 pKa = 11.21EE520 pKa = 3.71DD521 pKa = 3.98ANNINRR527 pKa = 11.84SLSIQPTAKK536 pKa = 10.08VPQLFGNFYY545 pKa = 10.63FSNKK549 pKa = 6.41HH550 pKa = 3.74TRR552 pKa = 11.84MMPVHH557 pKa = 6.54SVPGLEE563 pKa = 4.73RR564 pKa = 11.84II565 pKa = 4.02

Molecular weight: 63.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8RW86|F8RW86_9VIRU Uncharacterized protein p5 OS=Microviridae phi-CA82 OX=913970 GN=p5 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.58CNVDD6 pKa = 3.33NSTVIHH12 pKa = 5.68TLSTRR17 pKa = 11.84FPPNYY22 pKa = 9.89QHH24 pKa = 7.15GKK26 pKa = 7.96PLNHH30 pKa = 7.08KK31 pKa = 8.41GLKK34 pKa = 10.19RR35 pKa = 11.84FFTVSTLLLLLLRR48 pKa = 11.84KK49 pKa = 10.06YY50 pKa = 9.1ITIYY54 pKa = 10.2IYY56 pKa = 10.86NKK58 pKa = 9.78HH59 pKa = 5.92SRR61 pKa = 11.84QKK63 pKa = 9.05VTSDD67 pKa = 2.91NRR69 pKa = 11.84NVV71 pKa = 2.98

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

1669

39

565

166.9

19.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.351 ± 1.457

1.318 ± 0.427

5.752 ± 0.537

5.812 ± 0.857

2.936 ± 0.265

5.812 ± 0.595

2.157 ± 0.573

6.171 ± 1.142

8.149 ± 1.238

6.89 ± 0.534

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.415 ± 0.296

7.969 ± 0.926

3.835 ± 0.688

4.434 ± 0.942

4.793 ± 0.499

6.171 ± 0.604

6.651 ± 0.596

3.835 ± 0.584

1.738 ± 0.54

5.812 ± 0.738
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski