Cyanothece sp. (strain PCC 7425 / ATCC 29141)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Cyanothecaceae; Cyanothece; unclassified Cyanothece

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5232 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8HYH9|B8HYH9_CYAP4 Uncharacterized protein OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=Cyan7425_4323 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.87PLVSAPGPTATFTPALNRR20 pKa = 11.84WLVFSGQDD28 pKa = 3.43QLDD31 pKa = 4.12LLLEE35 pKa = 4.17QYY37 pKa = 9.8IHH39 pKa = 6.7SLYY42 pKa = 10.9DD43 pKa = 3.02QFLNHH48 pKa = 7.0PLKK51 pKa = 10.49PDD53 pKa = 4.17LSPDD57 pKa = 3.3QLSQLEE63 pKa = 4.54GYY65 pKa = 9.56DD66 pKa = 3.22WYY68 pKa = 10.82RR69 pKa = 11.84ASLTQGLNVEE79 pKa = 4.35EE80 pKa = 4.66LADD83 pKa = 5.23LVTNWLMAVQQMSDD97 pKa = 3.7LPLDD101 pKa = 3.89QPQQPP106 pKa = 3.7

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8HR60|B8HR60_CYAP4 Uncharacterized protein OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=Cyan7425_1679 PE=4 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTVRR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.12RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MKK24 pKa = 9.25TKK26 pKa = 9.8NGRR29 pKa = 11.84RR30 pKa = 11.84VIQARR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84GRR40 pKa = 11.84VRR42 pKa = 11.84LAVV45 pKa = 3.37

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5232

0

5232

1607800

29

3706

307.3

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.898 ± 0.034

1.069 ± 0.011

4.704 ± 0.024

5.88 ± 0.034

3.762 ± 0.022

6.866 ± 0.03

2.062 ± 0.018

5.811 ± 0.026

3.524 ± 0.027

12.107 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.013

3.398 ± 0.018

5.449 ± 0.032

6.158 ± 0.035

5.82 ± 0.025

6.022 ± 0.028

5.531 ± 0.023

6.59 ± 0.026

1.587 ± 0.015

2.919 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski