Staphylococcus phage SpaA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Beceayunavirus; unclassified Beceayunavirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7IDH3|I7IDH3_9CAUD Recombination protein U OS=Staphylococcus phage SpaA1 OX=1118063 GN=P53 PE=3 SV=1
MM1 pKa = 7.07KK2 pKa = 9.77TFTVTYY8 pKa = 9.15TEE10 pKa = 4.12TKK12 pKa = 8.24VYY14 pKa = 10.28EE15 pKa = 4.86GIVEE19 pKa = 4.46AEE21 pKa = 4.26TLEE24 pKa = 4.12QAADD28 pKa = 3.86RR29 pKa = 11.84VRR31 pKa = 11.84DD32 pKa = 4.09GYY34 pKa = 11.12VEE36 pKa = 4.31EE37 pKa = 4.27EE38 pKa = 4.32VLVEE42 pKa = 4.08KK43 pKa = 10.74DD44 pKa = 3.05ITIDD48 pKa = 3.62EE49 pKa = 4.73LKK51 pKa = 10.81LEE53 pKa = 4.25QTEE56 pKa = 4.25EE57 pKa = 4.13PDD59 pKa = 3.4NVRR62 pKa = 11.84SLVINYY68 pKa = 10.07GEE70 pKa = 3.98LSFTNFEE77 pKa = 4.07HH78 pKa = 6.51AVNVLSEE85 pKa = 4.33EE86 pKa = 4.26YY87 pKa = 10.74GFEE90 pKa = 4.14GEE92 pKa = 3.81AWEE95 pKa = 4.2MVVASGDD102 pKa = 3.89LEE104 pKa = 4.16ILCEE108 pKa = 4.06FLSEE112 pKa = 4.53DD113 pKa = 4.24GISAEE118 pKa = 4.41IEE120 pKa = 3.97

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7ILS7|I7ILS7_9CAUD Phage tail tape measure protein OS=Staphylococcus phage SpaA1 OX=1118063 GN=P17 PE=4 SV=1
MM1 pKa = 7.69SMKK4 pKa = 10.14FQSNRR9 pKa = 11.84AAVMARR15 pKa = 11.84HH16 pKa = 6.01LAAKK20 pKa = 9.57KK21 pKa = 9.61AAHH24 pKa = 5.72TAVGQFVSSKK34 pKa = 10.78AKK36 pKa = 10.47LLAAVDD42 pKa = 3.87TGNLRR47 pKa = 11.84SSISSKK53 pKa = 11.32AEE55 pKa = 3.67LEE57 pKa = 4.12KK58 pKa = 11.22VVIGTSADD66 pKa = 3.19YY67 pKa = 10.84GIYY70 pKa = 9.53VEE72 pKa = 5.28KK73 pKa = 10.12GTGVYY78 pKa = 10.35AVDD81 pKa = 3.37GDD83 pKa = 4.07GRR85 pKa = 11.84KK86 pKa = 7.31TPWMYY91 pKa = 11.02RR92 pKa = 11.84DD93 pKa = 3.52PKK95 pKa = 8.09TGKK98 pKa = 8.46MVKK101 pKa = 8.29TQGQHH106 pKa = 4.99AQPFLRR112 pKa = 11.84PAAEE116 pKa = 4.21NNKK119 pKa = 9.76PMITQAATRR128 pKa = 11.84TYY130 pKa = 10.64SSLMRR135 pKa = 5.16

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12473

38

1569

198.0

22.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.221 ± 0.562

0.818 ± 0.137

5.74 ± 0.268

8.474 ± 0.411

4.225 ± 0.179

5.925 ± 0.417

1.74 ± 0.168

7.312 ± 0.196

9.42 ± 0.288

7.873 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.006 ± 0.212

5.283 ± 0.195

2.854 ± 0.185

4.113 ± 0.254

4.434 ± 0.265

5.444 ± 0.163

5.564 ± 0.329

6.494 ± 0.232

1.323 ± 0.141

3.736 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski