Dragonfly associated cyclovirus 1
Average proteome isoelectric point is 8.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0A1L0|K0A1L0_9CIRC ATP-dependent helicase Rep OS=Dragonfly associated cyclovirus 1 OX=1234879 PE=3 SV=1
MM1 pKa = 7.68 NITIRR6 pKa = 11.84 RR7 pKa = 11.84 FVFTWNNYY15 pKa = 6.29 TPSDD19 pKa = 4.03 FEE21 pKa = 4.35 TCITFLDD28 pKa = 4.04 NFCKK32 pKa = 10.61 YY33 pKa = 10.82 GIIGKK38 pKa = 7.17 EE39 pKa = 3.92 KK40 pKa = 10.71 CPTTQTPHH48 pKa = 5.7 IQGFCNLSKK57 pKa = 10.71 PMRR60 pKa = 11.84 FNNIKK65 pKa = 10.11 KK66 pKa = 9.31 HH67 pKa = 4.77 LHH69 pKa = 5.25 NSIHH73 pKa = 6.15 IEE75 pKa = 3.86 KK76 pKa = 10.82 ANGSDD81 pKa = 3.74 EE82 pKa = 4.07 QNKK85 pKa = 9.78 IYY87 pKa = 10.22 CSKK90 pKa = 10.67 SGEE93 pKa = 4.21 FFEE96 pKa = 5.81 KK97 pKa = 10.57 GHH99 pKa = 7.03 PDD101 pKa = 3.19 KK102 pKa = 11.01 QGKK105 pKa = 9.94 RR106 pKa = 11.84 NDD108 pKa = 3.42 LDD110 pKa = 4.22 AVVLTIQNGTNTLSNVAKK128 pKa = 10.19 LHH130 pKa = 5.09 PTSFIKK136 pKa = 9.57 YY137 pKa = 7.8 HH138 pKa = 6.38 KK139 pKa = 10.15 GIKK142 pKa = 9.8 EE143 pKa = 4.3 YY144 pKa = 10.24 INHH147 pKa = 5.88 VNPIRR152 pKa = 11.84 PRR154 pKa = 11.84 HH155 pKa = 5.3 YY156 pKa = 9.85 KK157 pKa = 10.32 SEE159 pKa = 4.05 VYY161 pKa = 10.26 YY162 pKa = 10.54 YY163 pKa = 9.38 WGPPGTGKK171 pKa = 10.66 SKK173 pKa = 9.35 TALEE177 pKa = 4.15 KK178 pKa = 9.95 ATAYY182 pKa = 8.63 NTDD185 pKa = 3.35 SIYY188 pKa = 10.77 YY189 pKa = 9.5 KK190 pKa = 10.51 PRR192 pKa = 11.84 GLWWDD197 pKa = 4.8 GYY199 pKa = 7.48 QQQTSVIIDD208 pKa = 4.09 DD209 pKa = 4.39 FYY211 pKa = 11.87 GWIKK215 pKa = 10.75 YY216 pKa = 10.28 DD217 pKa = 4.1 EE218 pKa = 4.44 LLKK221 pKa = 10.71 ICDD224 pKa = 3.53 RR225 pKa = 11.84 YY226 pKa = 9.88 PYY228 pKa = 10.1 KK229 pKa = 10.94 VQIKK233 pKa = 10.43 GGFEE237 pKa = 4.14 EE238 pKa = 4.91 FTSKK242 pKa = 11.17 YY243 pKa = 10.6 IFITSNVDD251 pKa = 2.78 TCDD254 pKa = 3.07 LYY256 pKa = 11.4 KK257 pKa = 10.57 FRR259 pKa = 11.84 NYY261 pKa = 8.98 NTDD264 pKa = 3.51 AIEE267 pKa = 3.85 RR268 pKa = 11.84 RR269 pKa = 11.84 ITEE272 pKa = 4.45 KK273 pKa = 10.35 IHH275 pKa = 6.27 FKK277 pKa = 11.07 NIFF280 pKa = 3.23
Molecular weight: 32.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.527
IPC2_protein 8.478
IPC_protein 8.346
Toseland 8.829
ProMoST 8.829
Dawson 9.224
Bjellqvist 9.136
Wikipedia 9.385
Rodwell 9.37
Grimsley 9.238
Solomon 9.282
Lehninger 9.238
Nozaki 9.209
DTASelect 9.033
Thurlkill 9.107
EMBOSS 9.355
Sillero 9.297
Patrickios 4.139
IPC_peptide 9.268
IPC2_peptide 7.863
IPC2.peptide.svr19 7.713
Protein with the highest isoelectric point:
>tr|K0A1L0|K0A1L0_9CIRC ATP-dependent helicase Rep OS=Dragonfly associated cyclovirus 1 OX=1234879 PE=3 SV=1
MM1 pKa = 6.69 VRR3 pKa = 11.84 YY4 pKa = 9.2 RR5 pKa = 11.84 RR6 pKa = 11.84 AVRR9 pKa = 11.84 RR10 pKa = 11.84 PVRR13 pKa = 11.84 RR14 pKa = 11.84 ARR16 pKa = 11.84 RR17 pKa = 11.84 SRR19 pKa = 11.84 VRR21 pKa = 11.84 KK22 pKa = 9.26 LRR24 pKa = 11.84 FRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 9.3 HH32 pKa = 6.42 RR33 pKa = 11.84 RR34 pKa = 11.84 ITGNFSMKK42 pKa = 7.29 ITKK45 pKa = 9.11 MEE47 pKa = 4.48 SYY49 pKa = 10.82 SPDD52 pKa = 2.73 ISKK55 pKa = 10.39 VVEE58 pKa = 4.16 LPVTFTGKK66 pKa = 10.18 DD67 pKa = 3.41 FPEE70 pKa = 4.5 FQTIAPCFEE79 pKa = 4.68 AYY81 pKa = 9.41 RR82 pKa = 11.84 VHH84 pKa = 7.6 KK85 pKa = 8.83 ITCTVYY91 pKa = 8.62 PQQNISTRR99 pKa = 11.84 DD100 pKa = 3.15 QTTIMPAYY108 pKa = 10.71 CMFPWHH114 pKa = 6.61 GPVPAAQGKK123 pKa = 8.89 FNNFLSVDD131 pKa = 3.13 KK132 pKa = 10.97 CKK134 pKa = 10.67 VFRR137 pKa = 11.84 GTQVGFQTYY146 pKa = 9.2 VPNTLSTVYY155 pKa = 10.45 DD156 pKa = 3.33 IDD158 pKa = 4.18 GASSTTRR165 pKa = 11.84 TSWRR169 pKa = 11.84 PRR171 pKa = 11.84 IEE173 pKa = 4.1 VTSDD177 pKa = 2.91 DD178 pKa = 3.94 AYY180 pKa = 10.35 KK181 pKa = 9.6 VVHH184 pKa = 6.08 YY185 pKa = 8.73 GGCVGMQGIGDD196 pKa = 4.65 GLPKK200 pKa = 10.53 GATAHH205 pKa = 6.3 YY206 pKa = 8.86 NLKK209 pKa = 10.18 YY210 pKa = 10.52 DD211 pKa = 4.29 IYY213 pKa = 10.75 LTFYY217 pKa = 9.18 NQKK220 pKa = 9.76 IFLKK224 pKa = 10.77
Molecular weight: 26.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.794
IPC_protein 10.409
Toseland 10.306
ProMoST 10.101
Dawson 10.511
Bjellqvist 10.248
Wikipedia 10.73
Rodwell 10.789
Grimsley 10.599
Solomon 10.555
Lehninger 10.511
Nozaki 10.321
DTASelect 10.233
Thurlkill 10.365
EMBOSS 10.716
Sillero 10.438
Patrickios 10.35
IPC_peptide 10.555
IPC2_peptide 9.268
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
504
224
280
252.0
29.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.571 ± 0.845
2.381 ± 0.094
4.96 ± 0.313
3.77 ± 0.971
5.952 ± 0.094
6.151 ± 0.063
2.976 ± 0.47
7.738 ± 1.503
8.532 ± 1.44
3.968 ± 0.25
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.587 ± 0.689
5.556 ± 1.534
4.96 ± 0.532
3.571 ± 0.282
7.143 ± 2.536
5.159 ± 0.125
8.333 ± 0.376
5.556 ± 1.847
1.389 ± 0.313
6.746 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here