Butyrivibrio sp. M55

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; unclassified Butyrivibrio

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7JBP5|A0A1I7JBP5_9FIRM 3-methyladenine DNA glycosylase AlkD OS=Butyrivibrio sp. M55 OX=1855323 GN=SAMN05216540_11224 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.46RR3 pKa = 11.84NIALVLTCLMIIGMVGCVGNDD24 pKa = 3.11KK25 pKa = 11.21AQDD28 pKa = 3.57SEE30 pKa = 4.82NIGGANDD37 pKa = 3.47AASNEE42 pKa = 4.23LEE44 pKa = 4.26TTSAGDD50 pKa = 3.26EE51 pKa = 4.14AQAAEE56 pKa = 4.31AASEE60 pKa = 4.08QATAADD66 pKa = 4.93DD67 pKa = 5.35DD68 pKa = 4.12FTPYY72 pKa = 11.0QLFLSDD78 pKa = 4.85DD79 pKa = 4.04LSVDD83 pKa = 3.01GQTFLEE89 pKa = 5.03IYY91 pKa = 10.64AEE93 pKa = 4.16SFDD96 pKa = 4.95DD97 pKa = 5.03LGEE100 pKa = 4.25KK101 pKa = 10.25PEE103 pKa = 4.01AYY105 pKa = 10.26YY106 pKa = 11.24YY107 pKa = 10.99DD108 pKa = 3.79VDD110 pKa = 4.61SDD112 pKa = 4.93GEE114 pKa = 4.29DD115 pKa = 3.37EE116 pKa = 4.67LLVVGPYY123 pKa = 10.35YY124 pKa = 11.01GFDD127 pKa = 3.08IYY129 pKa = 11.58DD130 pKa = 3.71VINGEE135 pKa = 4.55FTCLDD140 pKa = 3.87CGGGIATICDD150 pKa = 3.41IYY152 pKa = 11.2AGEE155 pKa = 4.08GKK157 pKa = 10.43AYY159 pKa = 10.11VVHH162 pKa = 6.86TDD164 pKa = 4.02FGHH167 pKa = 6.45VGRR170 pKa = 11.84QNIEE174 pKa = 3.59FALYY178 pKa = 10.09GSNGEE183 pKa = 4.13KK184 pKa = 10.45VEE186 pKa = 4.12SFEE189 pKa = 4.84ISAGYY194 pKa = 8.69PDD196 pKa = 4.6KK197 pKa = 11.48DD198 pKa = 3.62NYY200 pKa = 11.11DD201 pKa = 3.89EE202 pKa = 5.68NSMFLLNDD210 pKa = 3.51KK211 pKa = 10.01EE212 pKa = 4.28ITMQEE217 pKa = 3.92YY218 pKa = 9.97EE219 pKa = 4.74KK220 pKa = 11.15YY221 pKa = 10.58LNTYY225 pKa = 9.52KK226 pKa = 10.83PLDD229 pKa = 3.75RR230 pKa = 11.84STLKK234 pKa = 10.86SAWDD238 pKa = 3.37

Molecular weight: 26.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7GZN7|A0A1I7GZN7_9FIRM Exodeoxyribonuclease III OS=Butyrivibrio sp. M55 OX=1855323 GN=SAMN05216540_103223 PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3341

0

3341

1100749

25

2500

329.5

36.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.24 ± 0.039

1.392 ± 0.016

6.527 ± 0.035

7.453 ± 0.048

4.374 ± 0.035

6.843 ± 0.034

1.527 ± 0.017

7.878 ± 0.043

7.634 ± 0.038

8.246 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.985 ± 0.02

4.916 ± 0.03

2.997 ± 0.024

2.538 ± 0.025

3.917 ± 0.033

6.241 ± 0.032

5.177 ± 0.029

6.923 ± 0.036

0.82 ± 0.013

4.371 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski