Hubei permutotetra-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KI36|A0A1L3KI36_9VIRU Capsid protein OS=Hubei permutotetra-like virus 9 OX=1923083 PE=3 SV=1
MM1 pKa = 7.11VNSEE5 pKa = 4.29GEE7 pKa = 4.19YY8 pKa = 10.09ILEE11 pKa = 4.59KK12 pKa = 10.4FQHH15 pKa = 5.85TPWNLHH21 pKa = 5.15PTPHH25 pKa = 5.68QVQGADD31 pKa = 3.68GYY33 pKa = 9.27SQYY36 pKa = 11.37VGWRR40 pKa = 11.84PSPSGPPTLFDD51 pKa = 3.64AVSLSVGNSAGFADD65 pKa = 5.64ASLQNLDD72 pKa = 3.51VEE74 pKa = 4.57EE75 pKa = 4.66PKK77 pKa = 10.71VDD79 pKa = 4.32FVFNLQPLDD88 pKa = 3.91LLQPNIPSIVRR99 pKa = 11.84LTFTLTFEE107 pKa = 4.4RR108 pKa = 11.84MNIPAEE114 pKa = 4.05EE115 pKa = 4.09NKK117 pKa = 10.4YY118 pKa = 11.15DD119 pKa = 3.57WVFDD123 pKa = 4.16LSDD126 pKa = 4.11LLDD129 pKa = 4.03HH130 pKa = 7.11VEE132 pKa = 4.13VLPTSSLVVNPHH144 pKa = 5.94LRR146 pKa = 11.84QFTKK150 pKa = 10.2TFWGFTKK157 pKa = 10.24RR158 pKa = 11.84YY159 pKa = 8.38ARR161 pKa = 11.84GYY163 pKa = 7.75IPKK166 pKa = 9.36VVVSMVSHH174 pKa = 6.67FGVEE178 pKa = 4.01AEE180 pKa = 4.12DD181 pKa = 3.8TFVGRR186 pKa = 11.84IRR188 pKa = 11.84GACEE192 pKa = 3.36WMSTSLALFTGPVVRR207 pKa = 11.84EE208 pKa = 3.94PARR211 pKa = 11.84SFAYY215 pKa = 10.01LWEE218 pKa = 4.68LNLLKK223 pKa = 10.33KK224 pKa = 10.28RR225 pKa = 11.84HH226 pKa = 5.84SRR228 pKa = 11.84PLDD231 pKa = 3.75PVDD234 pKa = 4.99DD235 pKa = 4.02SLEE238 pKa = 4.03FVLLDD243 pKa = 3.76ASS245 pKa = 3.81

Molecular weight: 27.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KI36|A0A1L3KI36_9VIRU Capsid protein OS=Hubei permutotetra-like virus 9 OX=1923083 PE=3 SV=1
MM1 pKa = 7.08TGRR4 pKa = 11.84RR5 pKa = 11.84ANSSSEE11 pKa = 3.76KK12 pKa = 10.28LKK14 pKa = 11.13RR15 pKa = 11.84EE16 pKa = 3.48IANLQRR22 pKa = 11.84RR23 pKa = 11.84LATSTISSNGPRR35 pKa = 11.84SGRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84GGNGNGGGGPSVPAAMGSNPRR63 pKa = 11.84PSRR66 pKa = 11.84GRR68 pKa = 11.84RR69 pKa = 11.84GGARR73 pKa = 11.84TRR75 pKa = 11.84VGNGGRR81 pKa = 11.84IVLTRR86 pKa = 11.84DD87 pKa = 3.06EE88 pKa = 4.88LLVTVATTPDD98 pKa = 3.32KK99 pKa = 11.42NEE101 pKa = 4.27SVFSKK106 pKa = 10.91DD107 pKa = 3.61LVPSPGVMPFLFRR120 pKa = 11.84LSSCYY125 pKa = 8.83QRR127 pKa = 11.84IRR129 pKa = 11.84WVRR132 pKa = 11.84ASISWRR138 pKa = 11.84PSCGTATDD146 pKa = 5.14GIISYY151 pKa = 10.33GVAFNGSEE159 pKa = 4.32SIKK162 pKa = 10.97SRR164 pKa = 11.84DD165 pKa = 3.56LVTSLTPCNDD175 pKa = 3.01HH176 pKa = 7.08PVWQSTGISPLVIPGDD192 pKa = 3.75MLMSRR197 pKa = 11.84RR198 pKa = 11.84WYY200 pKa = 8.95PLNVTGGDD208 pKa = 3.5QFDD211 pKa = 3.46KK212 pKa = 11.4GMGKK216 pKa = 9.69FCVGLTHH223 pKa = 7.65DD224 pKa = 4.25AEE226 pKa = 4.62KK227 pKa = 10.42VAKK230 pKa = 10.22SRR232 pKa = 11.84GEE234 pKa = 3.61FWISYY239 pKa = 5.18TVEE242 pKa = 3.96MEE244 pKa = 4.26GTNPAA249 pKa = 3.76

Molecular weight: 26.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1620

245

1126

540.0

60.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.79 ± 0.737

0.679 ± 0.277

5.37 ± 0.396

6.79 ± 1.101

4.198 ± 0.56

7.346 ± 1.502

1.852 ± 0.324

3.272 ± 0.338

4.753 ± 0.617

8.704 ± 0.841

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.177

3.025 ± 0.726

7.037 ± 0.202

3.333 ± 0.563

8.457 ± 0.955

7.284 ± 1.379

5.37 ± 0.462

8.457 ± 0.488

2.222 ± 0.068

2.716 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski