Salmonella phage vB_SnwM_CGG4-1
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 259 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B0VV34|A0A1B0VV34_9CAUD Exonuclease A OS=Salmonella phage vB_SnwM_CGG4-1 OX=1815631 GN=CGG41_012 PE=4 SV=1
MM1 pKa = 7.57 ISYY4 pKa = 10.93 SDD6 pKa = 4.22 IEE8 pKa = 4.26 NALIKK13 pKa = 10.67 YY14 pKa = 8.3 WGLNPLNPEE23 pKa = 4.47 DD24 pKa = 4.64 KK25 pKa = 10.1 YY26 pKa = 11.22 QSLRR30 pKa = 11.84 EE31 pKa = 4.22 LIGDD35 pKa = 4.02 SEE37 pKa = 5.56 DD38 pKa = 3.67 IIADD42 pKa = 3.16 IVEE45 pKa = 4.33 ALNNYY50 pKa = 8.74
Molecular weight: 5.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.923
IPC_protein 3.77
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A1B0VVP1|A0A1B0VVP1_9CAUD Uncharacterized protein OS=Salmonella phage vB_SnwM_CGG4-1 OX=1815631 GN=CGG41_242 PE=4 SV=1
MM1 pKa = 7.14 TRR3 pKa = 11.84 RR4 pKa = 11.84 TFLSIEE10 pKa = 4.14 TLAKK14 pKa = 10.57 AGATVIGTIKK24 pKa = 10.32 NADD27 pKa = 3.28 WVGGVPMEE35 pKa = 4.73 EE36 pKa = 4.17 EE37 pKa = 3.85 LSRR40 pKa = 11.84 PGFYY44 pKa = 10.42 FIMNNGGFAVSRR56 pKa = 11.84 SYY58 pKa = 11.22 VGRR61 pKa = 11.84 QRR63 pKa = 11.84 SKK65 pKa = 9.58 TGFSNTLSQFRR76 pKa = 11.84 QGRR79 pKa = 11.84 SQLGRR84 pKa = 11.84 TLSNNHH90 pKa = 6.88 RR91 pKa = 11.84 IYY93 pKa = 10.85 SVVFVPVEE101 pKa = 3.82 KK102 pKa = 10.23 MKK104 pKa = 10.52 PLTTGHH110 pKa = 6.68 GKK112 pKa = 9.88 GQLSLAFTRR121 pKa = 11.84 KK122 pKa = 10.03 HH123 pKa = 5.92 EE124 pKa = 4.4 DD125 pKa = 2.73 AFQNLEE131 pKa = 3.84 EE132 pKa = 4.33 MNRR135 pKa = 11.84 MLNDD139 pKa = 2.97 NFKK142 pKa = 11.06 FIMQKK147 pKa = 10.08 YY148 pKa = 8.59
Molecular weight: 16.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.648
IPC_protein 10.101
Toseland 10.555
ProMoST 10.175
Dawson 10.657
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 11.038
Grimsley 10.716
Solomon 10.73
Lehninger 10.701
Nozaki 10.511
DTASelect 10.321
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.584
Patrickios 10.774
IPC_peptide 10.73
IPC2_peptide 8.902
IPC2.peptide.svr19 8.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
259
0
259
50204
38
1273
193.8
21.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.563 ± 0.203
1.03 ± 0.069
6.28 ± 0.109
7.26 ± 0.159
4.322 ± 0.096
6.328 ± 0.205
1.737 ± 0.089
7.412 ± 0.155
7.489 ± 0.219
7.462 ± 0.131
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.751 ± 0.096
5.766 ± 0.126
3.446 ± 0.114
3.408 ± 0.109
4.275 ± 0.106
6.499 ± 0.137
5.788 ± 0.21
6.503 ± 0.142
1.456 ± 0.053
4.223 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here