Fragaria chiloensis cryptic virus
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4FCM7|Q4FCM7_9VIRU RNA-dependent RNA polymerase OS=Fragaria chiloensis cryptic virus OX=335204 PE=4 SV=2
MM1 pKa = 7.28 ATDD4 pKa = 3.43 NSDD7 pKa = 3.71 AKK9 pKa = 10.16 TEE11 pKa = 3.99 QSKK14 pKa = 8.04 VTEE17 pKa = 4.65 SKK19 pKa = 10.74 GSSPTKK25 pKa = 10.25 SLPAHH30 pKa = 6.32 AADD33 pKa = 3.82 EE34 pKa = 4.31 KK35 pKa = 10.87 AAVGATFKK43 pKa = 10.84 LQSRR47 pKa = 11.84 QPTEE51 pKa = 3.87 PPAIPSVFAQSQSLNDD67 pKa = 3.67 SVGNPDD73 pKa = 5.6 FIAQLRR79 pKa = 11.84 DD80 pKa = 2.83 EE81 pKa = 4.49 GMYY84 pKa = 11.23 GLFLPRR90 pKa = 11.84 DD91 pKa = 3.58 RR92 pKa = 11.84 ARR94 pKa = 11.84 RR95 pKa = 11.84 TPTPVTLRR103 pKa = 11.84 PLTYY107 pKa = 9.25 FQSIEE112 pKa = 4.06 RR113 pKa = 11.84 TISQSMFHH121 pKa = 7.13 LLQTKK126 pKa = 8.22 TEE128 pKa = 4.19 FNNDD132 pKa = 3.2 DD133 pKa = 4.3 AIQRR137 pKa = 11.84 INTIAEE143 pKa = 3.78 QLAIGACLATHH154 pKa = 6.29 MKK156 pKa = 10.0 LRR158 pKa = 11.84 ALIHH162 pKa = 6.8 LDD164 pKa = 3.36 FPDD167 pKa = 4.2 RR168 pKa = 11.84 YY169 pKa = 10.86 NIGSLKK175 pKa = 10.11 RR176 pKa = 11.84 PKK178 pKa = 10.08 TITDD182 pKa = 3.93 LPVVAPFAFAIQQLGYY198 pKa = 11.3 VNIANLTEE206 pKa = 3.69 EE207 pKa = 3.98 RR208 pKa = 11.84 RR209 pKa = 11.84 YY210 pKa = 10.9 VPVLPEE216 pKa = 3.91 TGHH219 pKa = 5.96 TFGIPTGHH227 pKa = 6.23 NWNPNLYY234 pKa = 9.54 AQAVDD239 pKa = 3.58 YY240 pKa = 10.92 ARR242 pKa = 11.84 KK243 pKa = 9.54 FGLHH247 pKa = 6.16 FNVVDD252 pKa = 3.72 YY253 pKa = 9.62 TKK255 pKa = 10.85 KK256 pKa = 10.47 QGTAWWLLRR265 pKa = 11.84 QHH267 pKa = 6.62 FEE269 pKa = 4.24 DD270 pKa = 6.8 GIFEE274 pKa = 4.4 LQLPLPEE281 pKa = 4.49 VNFTSSMALTLSLFLNAEE299 pKa = 4.3 EE300 pKa = 4.44 VNATSEE306 pKa = 4.24 IFDD309 pKa = 4.23 LTPVGADD316 pKa = 2.36 IYY318 pKa = 10.33 GYY320 pKa = 10.43 IMRR323 pKa = 11.84 EE324 pKa = 3.52 PHH326 pKa = 6.48 LGINVSTFEE335 pKa = 5.17 VIDD338 pKa = 4.13 EE339 pKa = 4.24 SAKK342 pKa = 10.58 EE343 pKa = 3.87 IVSNVV348 pKa = 2.9
Molecular weight: 38.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.487
IPC2_protein 5.423
IPC_protein 5.423
Toseland 5.728
ProMoST 5.677
Dawson 5.575
Bjellqvist 5.626
Wikipedia 5.525
Rodwell 5.537
Grimsley 5.868
Solomon 5.575
Lehninger 5.55
Nozaki 5.792
DTASelect 5.957
Thurlkill 5.868
EMBOSS 5.83
Sillero 5.881
Patrickios 4.24
IPC_peptide 5.588
IPC2_peptide 5.881
IPC2.peptide.svr19 5.85
Protein with the highest isoelectric point:
>tr|Q4FCM7|Q4FCM7_9VIRU RNA-dependent RNA polymerase OS=Fragaria chiloensis cryptic virus OX=335204 PE=4 SV=2
MM1 pKa = 7.11 EE2 pKa = 4.95 HH3 pKa = 7.37 RR4 pKa = 11.84 FRR6 pKa = 11.84 GIPRR10 pKa = 11.84 GLIEE14 pKa = 5.14 LEE16 pKa = 4.32 EE17 pKa = 4.61 IPTRR21 pKa = 11.84 RR22 pKa = 11.84 LRR24 pKa = 11.84 EE25 pKa = 3.86 EE26 pKa = 3.96 RR27 pKa = 11.84 VVHH30 pKa = 6.15 IDD32 pKa = 2.81 AWSSKK37 pKa = 10.52 AIDD40 pKa = 4.66 AIVPLSLRR48 pKa = 11.84 IEE50 pKa = 4.27 LDD52 pKa = 2.72 GWARR56 pKa = 11.84 SYY58 pKa = 10.12 YY59 pKa = 9.73 TLQAHH64 pKa = 7.29 IDD66 pKa = 3.69 SIMQYY71 pKa = 10.73 DD72 pKa = 4.34 RR73 pKa = 11.84 PKK75 pKa = 10.83 LPQPTNAAWNTTTQHH90 pKa = 5.49 VRR92 pKa = 11.84 TQFARR97 pKa = 11.84 MDD99 pKa = 3.5 KK100 pKa = 10.52 VQTLSYY106 pKa = 10.28 LQLDD110 pKa = 3.44 QVKK113 pKa = 8.55 WVRR116 pKa = 11.84 SSAAGYY122 pKa = 10.1 GYY124 pKa = 10.84 VGRR127 pKa = 11.84 KK128 pKa = 8.82 SDD130 pKa = 3.29 NDD132 pKa = 3.18 NYY134 pKa = 10.4 FRR136 pKa = 11.84 ARR138 pKa = 11.84 KK139 pKa = 6.97 TAFTIAEE146 pKa = 4.41 KK147 pKa = 10.75 LNHH150 pKa = 6.4 DD151 pKa = 3.96 RR152 pKa = 11.84 DD153 pKa = 4.13 YY154 pKa = 11.81 GPLALEE160 pKa = 5.24 DD161 pKa = 4.09 STPDD165 pKa = 2.95 IAFTRR170 pKa = 11.84 TQLCQIKK177 pKa = 10.09 VKK179 pKa = 10.68 RR180 pKa = 11.84 KK181 pKa = 8.85 IRR183 pKa = 11.84 NVWGEE188 pKa = 3.7 AFHH191 pKa = 6.65 YY192 pKa = 10.62 VLLEE196 pKa = 3.85 GLFADD201 pKa = 5.87 PIIQHH206 pKa = 6.73 FIRR209 pKa = 11.84 NKK211 pKa = 8.32 SFYY214 pKa = 10.66 FIGEE218 pKa = 4.28 DD219 pKa = 3.35 PLLAVPRR226 pKa = 11.84 LVEE229 pKa = 4.85 KK230 pKa = 10.51 ILSEE234 pKa = 3.71 QDD236 pKa = 3.3 YY237 pKa = 11.07 VYY239 pKa = 10.61 MFDD242 pKa = 3.19 WSGFDD247 pKa = 3.73 ASVQEE252 pKa = 4.0 WEE254 pKa = 3.77 IRR256 pKa = 11.84 FAFSLLEE263 pKa = 4.4 SILIFPSSVEE273 pKa = 3.88 SYY275 pKa = 8.34 IWHH278 pKa = 6.89 FIIEE282 pKa = 4.06 LFIYY286 pKa = 10.16 RR287 pKa = 11.84 KK288 pKa = 9.36 IAAPNGKK295 pKa = 9.81 VYY297 pKa = 10.9 LKK299 pKa = 9.95 TLGIPSGSCFTNIIGSIVNYY319 pKa = 10.14 VRR321 pKa = 11.84 IQYY324 pKa = 10.66 LFFRR328 pKa = 11.84 LTNNFVTVFTHH339 pKa = 7.41 GDD341 pKa = 3.4 DD342 pKa = 3.78 SLVGVSTTQYY352 pKa = 9.38 VQMDD356 pKa = 4.15 NFEE359 pKa = 5.14 PICAEE364 pKa = 4.14 HH365 pKa = 6.52 NWTINIAKK373 pKa = 9.99 SAVSHH378 pKa = 5.51 EE379 pKa = 4.3 AEE381 pKa = 3.98 GVSFLSRR388 pKa = 11.84 KK389 pKa = 8.61 VRR391 pKa = 11.84 EE392 pKa = 4.09 HH393 pKa = 5.41 CHH395 pKa = 6.31 ARR397 pKa = 11.84 DD398 pKa = 3.3 EE399 pKa = 4.34 LLCLRR404 pKa = 11.84 MLKK407 pKa = 10.32 FPEE410 pKa = 4.13 YY411 pKa = 9.64 VVEE414 pKa = 4.33 SGAMSTLRR422 pKa = 11.84 AHH424 pKa = 7.62 SIHH427 pKa = 6.47 QDD429 pKa = 2.59 AGINSRR435 pKa = 11.84 YY436 pKa = 9.78 LYY438 pKa = 10.51 SIYY441 pKa = 10.45 KK442 pKa = 9.67 YY443 pKa = 10.62 LLHH446 pKa = 7.45 RR447 pKa = 11.84 YY448 pKa = 8.49 GKK450 pKa = 10.52 ADD452 pKa = 3.81 SLPLNQQNWDD462 pKa = 3.52 PLEE465 pKa = 4.09 YY466 pKa = 10.65 EE467 pKa = 3.99 NLRR470 pKa = 11.84 VSFATQNYY478 pKa = 6.39 EE479 pKa = 3.61
Molecular weight: 55.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.502
IPC2_protein 6.491
IPC_protein 6.649
Toseland 6.883
ProMoST 6.898
Dawson 7.059
Bjellqvist 6.985
Wikipedia 7.059
Rodwell 7.044
Grimsley 7.029
Solomon 7.073
Lehninger 7.073
Nozaki 7.293
DTASelect 7.424
Thurlkill 7.468
EMBOSS 7.468
Sillero 7.439
Patrickios 4.075
IPC_peptide 7.088
IPC2_peptide 7.102
IPC2.peptide.svr19 6.962
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1173
346
479
391.0
44.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.355 ± 0.867
1.108 ± 0.395
5.286 ± 0.08
6.223 ± 0.075
4.604 ± 0.773
4.263 ± 0.328
2.899 ± 0.214
7.076 ± 0.406
4.007 ± 0.103
9.037 ± 0.262
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.876 ± 0.288
4.689 ± 0.254
5.541 ± 0.872
4.007 ± 0.461
6.394 ± 0.564
6.479 ± 0.45
6.564 ± 0.859
5.797 ± 0.262
1.449 ± 0.314
4.348 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here