Fragaria chiloensis cryptic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q4FCM7|Q4FCM7_9VIRU RNA-dependent RNA polymerase OS=Fragaria chiloensis cryptic virus OX=335204 PE=4 SV=2
MM1 pKa = 7.28ATDD4 pKa = 3.43NSDD7 pKa = 3.71AKK9 pKa = 10.16TEE11 pKa = 3.99QSKK14 pKa = 8.04VTEE17 pKa = 4.65SKK19 pKa = 10.74GSSPTKK25 pKa = 10.25SLPAHH30 pKa = 6.32AADD33 pKa = 3.82EE34 pKa = 4.31KK35 pKa = 10.87AAVGATFKK43 pKa = 10.84LQSRR47 pKa = 11.84QPTEE51 pKa = 3.87PPAIPSVFAQSQSLNDD67 pKa = 3.67SVGNPDD73 pKa = 5.6FIAQLRR79 pKa = 11.84DD80 pKa = 2.83EE81 pKa = 4.49GMYY84 pKa = 11.23GLFLPRR90 pKa = 11.84DD91 pKa = 3.58RR92 pKa = 11.84ARR94 pKa = 11.84RR95 pKa = 11.84TPTPVTLRR103 pKa = 11.84PLTYY107 pKa = 9.25FQSIEE112 pKa = 4.06RR113 pKa = 11.84TISQSMFHH121 pKa = 7.13LLQTKK126 pKa = 8.22TEE128 pKa = 4.19FNNDD132 pKa = 3.2DD133 pKa = 4.3AIQRR137 pKa = 11.84INTIAEE143 pKa = 3.78QLAIGACLATHH154 pKa = 6.29MKK156 pKa = 10.0LRR158 pKa = 11.84ALIHH162 pKa = 6.8LDD164 pKa = 3.36FPDD167 pKa = 4.2RR168 pKa = 11.84YY169 pKa = 10.86NIGSLKK175 pKa = 10.11RR176 pKa = 11.84PKK178 pKa = 10.08TITDD182 pKa = 3.93LPVVAPFAFAIQQLGYY198 pKa = 11.3VNIANLTEE206 pKa = 3.69EE207 pKa = 3.98RR208 pKa = 11.84RR209 pKa = 11.84YY210 pKa = 10.9VPVLPEE216 pKa = 3.91TGHH219 pKa = 5.96TFGIPTGHH227 pKa = 6.23NWNPNLYY234 pKa = 9.54AQAVDD239 pKa = 3.58YY240 pKa = 10.92ARR242 pKa = 11.84KK243 pKa = 9.54FGLHH247 pKa = 6.16FNVVDD252 pKa = 3.72YY253 pKa = 9.62TKK255 pKa = 10.85KK256 pKa = 10.47QGTAWWLLRR265 pKa = 11.84QHH267 pKa = 6.62FEE269 pKa = 4.24DD270 pKa = 6.8GIFEE274 pKa = 4.4LQLPLPEE281 pKa = 4.49VNFTSSMALTLSLFLNAEE299 pKa = 4.3EE300 pKa = 4.44VNATSEE306 pKa = 4.24IFDD309 pKa = 4.23LTPVGADD316 pKa = 2.36IYY318 pKa = 10.33GYY320 pKa = 10.43IMRR323 pKa = 11.84EE324 pKa = 3.52PHH326 pKa = 6.48LGINVSTFEE335 pKa = 5.17VIDD338 pKa = 4.13EE339 pKa = 4.24SAKK342 pKa = 10.58EE343 pKa = 3.87IVSNVV348 pKa = 2.9

Molecular weight:
38.84 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q4FCM7|Q4FCM7_9VIRU RNA-dependent RNA polymerase OS=Fragaria chiloensis cryptic virus OX=335204 PE=4 SV=2
MM1 pKa = 7.11EE2 pKa = 4.95HH3 pKa = 7.37RR4 pKa = 11.84FRR6 pKa = 11.84GIPRR10 pKa = 11.84GLIEE14 pKa = 5.14LEE16 pKa = 4.32EE17 pKa = 4.61IPTRR21 pKa = 11.84RR22 pKa = 11.84LRR24 pKa = 11.84EE25 pKa = 3.86EE26 pKa = 3.96RR27 pKa = 11.84VVHH30 pKa = 6.15IDD32 pKa = 2.81AWSSKK37 pKa = 10.52AIDD40 pKa = 4.66AIVPLSLRR48 pKa = 11.84IEE50 pKa = 4.27LDD52 pKa = 2.72GWARR56 pKa = 11.84SYY58 pKa = 10.12YY59 pKa = 9.73TLQAHH64 pKa = 7.29IDD66 pKa = 3.69SIMQYY71 pKa = 10.73DD72 pKa = 4.34RR73 pKa = 11.84PKK75 pKa = 10.83LPQPTNAAWNTTTQHH90 pKa = 5.49VRR92 pKa = 11.84TQFARR97 pKa = 11.84MDD99 pKa = 3.5KK100 pKa = 10.52VQTLSYY106 pKa = 10.28LQLDD110 pKa = 3.44QVKK113 pKa = 8.55WVRR116 pKa = 11.84SSAAGYY122 pKa = 10.1GYY124 pKa = 10.84VGRR127 pKa = 11.84KK128 pKa = 8.82SDD130 pKa = 3.29NDD132 pKa = 3.18NYY134 pKa = 10.4FRR136 pKa = 11.84ARR138 pKa = 11.84KK139 pKa = 6.97TAFTIAEE146 pKa = 4.41KK147 pKa = 10.75LNHH150 pKa = 6.4DD151 pKa = 3.96RR152 pKa = 11.84DD153 pKa = 4.13YY154 pKa = 11.81GPLALEE160 pKa = 5.24DD161 pKa = 4.09STPDD165 pKa = 2.95IAFTRR170 pKa = 11.84TQLCQIKK177 pKa = 10.09VKK179 pKa = 10.68RR180 pKa = 11.84KK181 pKa = 8.85IRR183 pKa = 11.84NVWGEE188 pKa = 3.7AFHH191 pKa = 6.65YY192 pKa = 10.62VLLEE196 pKa = 3.85GLFADD201 pKa = 5.87PIIQHH206 pKa = 6.73FIRR209 pKa = 11.84NKK211 pKa = 8.32SFYY214 pKa = 10.66FIGEE218 pKa = 4.28DD219 pKa = 3.35PLLAVPRR226 pKa = 11.84LVEE229 pKa = 4.85KK230 pKa = 10.51ILSEE234 pKa = 3.71QDD236 pKa = 3.3YY237 pKa = 11.07VYY239 pKa = 10.61MFDD242 pKa = 3.19WSGFDD247 pKa = 3.73ASVQEE252 pKa = 4.0WEE254 pKa = 3.77IRR256 pKa = 11.84FAFSLLEE263 pKa = 4.4SILIFPSSVEE273 pKa = 3.88SYY275 pKa = 8.34IWHH278 pKa = 6.89FIIEE282 pKa = 4.06LFIYY286 pKa = 10.16RR287 pKa = 11.84KK288 pKa = 9.36IAAPNGKK295 pKa = 9.81VYY297 pKa = 10.9LKK299 pKa = 9.95TLGIPSGSCFTNIIGSIVNYY319 pKa = 10.14VRR321 pKa = 11.84IQYY324 pKa = 10.66LFFRR328 pKa = 11.84LTNNFVTVFTHH339 pKa = 7.41GDD341 pKa = 3.4DD342 pKa = 3.78SLVGVSTTQYY352 pKa = 9.38VQMDD356 pKa = 4.15NFEE359 pKa = 5.14PICAEE364 pKa = 4.14HH365 pKa = 6.52NWTINIAKK373 pKa = 9.99SAVSHH378 pKa = 5.51EE379 pKa = 4.3AEE381 pKa = 3.98GVSFLSRR388 pKa = 11.84KK389 pKa = 8.61VRR391 pKa = 11.84EE392 pKa = 4.09HH393 pKa = 5.41CHH395 pKa = 6.31ARR397 pKa = 11.84DD398 pKa = 3.3EE399 pKa = 4.34LLCLRR404 pKa = 11.84MLKK407 pKa = 10.32FPEE410 pKa = 4.13YY411 pKa = 9.64VVEE414 pKa = 4.33SGAMSTLRR422 pKa = 11.84AHH424 pKa = 7.62SIHH427 pKa = 6.47QDD429 pKa = 2.59AGINSRR435 pKa = 11.84YY436 pKa = 9.78LYY438 pKa = 10.51SIYY441 pKa = 10.45KK442 pKa = 9.67YY443 pKa = 10.62LLHH446 pKa = 7.45RR447 pKa = 11.84YY448 pKa = 8.49GKK450 pKa = 10.52ADD452 pKa = 3.81SLPLNQQNWDD462 pKa = 3.52PLEE465 pKa = 4.09YY466 pKa = 10.65EE467 pKa = 3.99NLRR470 pKa = 11.84VSFATQNYY478 pKa = 6.39EE479 pKa = 3.61

Molecular weight:
55.78 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1173

346

479

391.0

44.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.355 ± 0.867

1.108 ± 0.395

5.286 ± 0.08

6.223 ± 0.075

4.604 ± 0.773

4.263 ± 0.328

2.899 ± 0.214

7.076 ± 0.406

4.007 ± 0.103

9.037 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.876 ± 0.288

4.689 ± 0.254

5.541 ± 0.872

4.007 ± 0.461

6.394 ± 0.564

6.479 ± 0.45

6.564 ± 0.859

5.797 ± 0.262

1.449 ± 0.314

4.348 ± 0.608

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski