Streptococcus satellite phage Javan745

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZVV5|A0A4D5ZVV5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan745 OX=2558839 GN=JavanS745_0017 PE=4 SV=1
MM1 pKa = 7.62SLGNKK6 pKa = 9.5QIMADD11 pKa = 3.6NIKK14 pKa = 10.49RR15 pKa = 11.84LLSAKK20 pKa = 9.83GLNPRR25 pKa = 11.84QLAIALDD32 pKa = 4.26FKK34 pKa = 10.84YY35 pKa = 7.62TTVNDD40 pKa = 3.51WVNAKK45 pKa = 9.2TYY47 pKa = 10.43PRR49 pKa = 11.84IDD51 pKa = 4.22KK52 pKa = 10.71IEE54 pKa = 3.81MLANFFNVSKK64 pKa = 11.08SDD66 pKa = 3.61LVEE69 pKa = 4.22NKK71 pKa = 9.96NAEE74 pKa = 4.3TPTTSPIQSIYY85 pKa = 10.88DD86 pKa = 3.27QLTPPRR92 pKa = 11.84QEE94 pKa = 3.78KK95 pKa = 10.64ALTYY99 pKa = 10.35LKK101 pKa = 10.55KK102 pKa = 10.37QLLEE106 pKa = 4.02QKK108 pKa = 10.32NEE110 pKa = 4.21NIVSEE115 pKa = 4.5NIISLDD121 pKa = 3.66DD122 pKa = 3.6YY123 pKa = 11.31RR124 pKa = 11.84EE125 pKa = 4.27SKK127 pKa = 8.86TLPVIGVVTAGNGITQDD144 pKa = 3.61DD145 pKa = 4.02NLNIEE150 pKa = 4.02KK151 pKa = 10.39CFYY154 pKa = 10.27TDD156 pKa = 5.57EE157 pKa = 5.49IPDD160 pKa = 4.38DD161 pKa = 4.3YY162 pKa = 11.82DD163 pKa = 5.57AIAYY167 pKa = 8.54VVGNSMEE174 pKa = 4.18PKK176 pKa = 9.97IKK178 pKa = 10.88NGDD181 pKa = 3.4YY182 pKa = 11.13LFIKK186 pKa = 8.35NTPQVDD192 pKa = 3.97YY193 pKa = 8.82NTIGIFQVDD202 pKa = 3.58GANYY206 pKa = 9.54VKK208 pKa = 10.81KK209 pKa = 10.47LRR211 pKa = 11.84QGYY214 pKa = 9.62LEE216 pKa = 4.22SLNPEE221 pKa = 4.27CADD224 pKa = 3.24IQLDD228 pKa = 3.77EE229 pKa = 5.74DD230 pKa = 3.79NDD232 pKa = 3.21IRR234 pKa = 11.84TIGEE238 pKa = 3.96VVSIYY243 pKa = 10.62RR244 pKa = 11.84EE245 pKa = 3.88NN246 pKa = 3.2

Molecular weight: 27.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0H6|A0A4D6A0H6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan745 OX=2558839 GN=JavanS745_0011 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 9.27ITEE5 pKa = 4.16YY6 pKa = 10.16TKK8 pKa = 10.88KK9 pKa = 10.64DD10 pKa = 2.96GTTVYY15 pKa = 10.26RR16 pKa = 11.84SSVYY20 pKa = 10.68LGIDD24 pKa = 3.15TVTGKK29 pKa = 10.24KK30 pKa = 10.27VKK32 pKa = 8.07TTISGRR38 pKa = 11.84TKK40 pKa = 10.61RR41 pKa = 11.84EE42 pKa = 3.76LKK44 pKa = 10.7AKK46 pKa = 10.16ALQAQIDD53 pKa = 4.1FEE55 pKa = 5.07KK56 pKa = 11.04GGSTVYY62 pKa = 10.52KK63 pKa = 10.06AVEE66 pKa = 3.74IKK68 pKa = 9.8TYY70 pKa = 11.23AEE72 pKa = 4.37LVEE75 pKa = 4.62NWLEE79 pKa = 4.57TYY81 pKa = 10.53CHH83 pKa = 5.45TVKK86 pKa = 10.81KK87 pKa = 9.8ATLTIIKK94 pKa = 10.16SRR96 pKa = 11.84LNNYY100 pKa = 8.32LLPAFGEE107 pKa = 4.31YY108 pKa = 10.55KK109 pKa = 10.15LDD111 pKa = 3.64KK112 pKa = 9.63LTPPVIQKK120 pKa = 9.14QVNQWAKK127 pKa = 10.6EE128 pKa = 3.84YY129 pKa = 9.72NQLGKK134 pKa = 10.69GYY136 pKa = 9.89QEE138 pKa = 3.99YY139 pKa = 9.14PQLNSLNKK147 pKa = 10.15RR148 pKa = 11.84ILKK151 pKa = 9.76YY152 pKa = 10.38AVSLQVIPFNPARR165 pKa = 11.84DD166 pKa = 3.53IIVPRR171 pKa = 11.84RR172 pKa = 11.84KK173 pKa = 9.74EE174 pKa = 3.8KK175 pKa = 10.62EE176 pKa = 4.12GQKK179 pKa = 10.54LKK181 pKa = 11.2YY182 pKa = 10.43LDD184 pKa = 4.8DD185 pKa = 5.74DD186 pKa = 4.0NLKK189 pKa = 10.77KK190 pKa = 10.58FLTYY194 pKa = 10.66LEE196 pKa = 4.38QLPNTYY202 pKa = 10.74KK203 pKa = 10.78NFYY206 pKa = 9.48DD207 pKa = 3.51TVLYY211 pKa = 8.15KK212 pKa = 10.45TLLATGLRR220 pKa = 11.84IRR222 pKa = 11.84EE223 pKa = 4.08CLALKK228 pKa = 10.05WSDD231 pKa = 3.08IDD233 pKa = 4.02LQNGTLDD240 pKa = 3.42VNKK243 pKa = 9.41TLNCEE248 pKa = 4.2KK249 pKa = 10.44EE250 pKa = 4.29VTSPKK255 pKa = 8.61TKK257 pKa = 10.49SSIRR261 pKa = 11.84VIDD264 pKa = 4.28LDD266 pKa = 3.81NKK268 pKa = 8.09TVLMLRR274 pKa = 11.84LYY276 pKa = 10.7KK277 pKa = 10.34NRR279 pKa = 11.84QAQVGRR285 pKa = 11.84EE286 pKa = 3.53IGLTYY291 pKa = 10.68EE292 pKa = 4.41KK293 pKa = 10.78VFSNSFDD300 pKa = 3.44EE301 pKa = 4.33YY302 pKa = 10.78RR303 pKa = 11.84DD304 pKa = 3.44ARR306 pKa = 11.84ALRR309 pKa = 11.84SRR311 pKa = 11.84LEE313 pKa = 3.85KK314 pKa = 10.32HH315 pKa = 6.43LKK317 pKa = 10.66LSDD320 pKa = 3.61CPRR323 pKa = 11.84LTFHH327 pKa = 7.8AFRR330 pKa = 11.84HH331 pKa = 4.54THH333 pKa = 6.71ASILLNAGLPYY344 pKa = 10.43KK345 pKa = 10.2EE346 pKa = 3.68IQTRR350 pKa = 11.84LGHH353 pKa = 6.71AKK355 pKa = 10.54LSMTMDD361 pKa = 3.71IYY363 pKa = 11.79SHH365 pKa = 7.09LSKK368 pKa = 10.88DD369 pKa = 3.45NKK371 pKa = 10.49KK372 pKa = 10.17NATSFYY378 pKa = 9.85EE379 pKa = 3.99KK380 pKa = 10.33AIEE383 pKa = 4.04KK384 pKa = 10.35LKK386 pKa = 11.16SSS388 pKa = 3.82

Molecular weight: 44.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2883

50

388

144.2

16.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.099 ± 0.35

0.798 ± 0.089

6.001 ± 0.554

8.29 ± 0.633

3.607 ± 0.456

4.301 ± 0.342

1.318 ± 0.262

7.145 ± 0.435

10.753 ± 0.656

10.406 ± 0.544

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.247

6.382 ± 0.524

2.463 ± 0.278

4.024 ± 0.275

4.37 ± 0.367

6.452 ± 0.516

6.348 ± 0.564

4.821 ± 0.311

0.902 ± 0.19

4.232 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski