Paenibacillus sp. FSL R5-0912

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089K6E9|A0A089K6E9_9BACL Lipoprotein LpqB OS=Paenibacillus sp. FSL R5-0912 OX=1536771 GN=R50912_27615 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84PIIEE6 pKa = 4.46FCASNIGQGTEE17 pKa = 3.58KK18 pKa = 10.65LKK20 pKa = 11.28NKK22 pKa = 10.24LEE24 pKa = 4.09QNPEE28 pKa = 4.0YY29 pKa = 11.02DD30 pKa = 3.67VVEE33 pKa = 4.24YY34 pKa = 11.15GCLNNCGQCYY44 pKa = 8.87LQPFAMVDD52 pKa = 3.47GEE54 pKa = 4.33IIEE57 pKa = 4.99AEE59 pKa = 4.21SADD62 pKa = 3.65GLEE65 pKa = 4.28EE66 pKa = 5.26AIEE69 pKa = 4.02AAIKK73 pKa = 9.83EE74 pKa = 4.13AEE76 pKa = 4.08AWDD79 pKa = 3.95SLEE82 pKa = 4.16IDD84 pKa = 3.4

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089K860|A0A089K860_9BACL dTDP-4-dehydrorhamnose 3 5-epimerase OS=Paenibacillus sp. FSL R5-0912 OX=1536771 GN=R50912_31175 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5837

0

5837

1915437

26

2993

328.2

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.575 ± 0.041

0.754 ± 0.01

4.982 ± 0.024

6.76 ± 0.038

4.042 ± 0.023

7.739 ± 0.038

2.011 ± 0.016

6.573 ± 0.029

4.95 ± 0.028

10.492 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.014

3.746 ± 0.025

4.06 ± 0.021

3.826 ± 0.021

4.997 ± 0.027

6.487 ± 0.024

5.397 ± 0.033

6.93 ± 0.027

1.243 ± 0.015

3.634 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski