Theileria equi strain WA
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5313 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0AZ63|L0AZ63_THEEQ 60S acidic ribosomal protein P0 OS=Theileria equi strain WA OX=1537102 GN=BEWA_031590 PE=3 SV=1
MM1 pKa = 7.77 DD2 pKa = 5.21 LCWLEE7 pKa = 4.28 GQILDD12 pKa = 3.88 YY13 pKa = 11.59 ADD15 pKa = 3.31 ITRR18 pKa = 11.84 LPSTHH23 pKa = 6.4 YY24 pKa = 10.98 NLLILGDD31 pKa = 3.58 VDD33 pKa = 6.42 LILDD37 pKa = 3.65 SFKK40 pKa = 11.16 AVNPSLEE47 pKa = 4.31 NIVV50 pKa = 3.26
Molecular weight: 5.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.923
IPC_protein 3.783
Toseland 3.592
ProMoST 4.012
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.923
Patrickios 1.926
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|L0AW90|L0AW90_THEEQ Uncharacterized protein OS=Theileria equi strain WA OX=1537102 GN=BEWA_023520 PE=4 SV=1
MM1 pKa = 7.58 TKK3 pKa = 10.46 LQFRR7 pKa = 11.84 PATPFGISRR16 pKa = 11.84 PLQAPRR22 pKa = 11.84 APHH25 pKa = 6.17 FDD27 pKa = 3.69 SYY29 pKa = 10.95 TLSSTTDD36 pKa = 3.34 CQTMRR41 pKa = 11.84 KK42 pKa = 8.32 VKK44 pKa = 10.07 KK45 pKa = 9.56 PEE47 pKa = 3.62 RR48 pKa = 11.84 EE49 pKa = 3.96 RR50 pKa = 11.84 MCKK53 pKa = 9.86 RR54 pKa = 11.84 GEE56 pKa = 3.72 PWGPGGPQVSVGLTLWSHH74 pKa = 6.45 RR75 pKa = 11.84 PFFVGKK81 pKa = 9.47 IFSKK85 pKa = 9.27 EE86 pKa = 4.05 THH88 pKa = 5.81 LHH90 pKa = 5.48 EE91 pKa = 4.23 QTDD94 pKa = 3.7 MNRR97 pKa = 11.84 NLIVLRR103 pKa = 11.84 YY104 pKa = 8.91 LYY106 pKa = 10.2 WSIQVLAGIGASLYY120 pKa = 10.83 GAGSNGNSSNWQCLGIAFLVTGVAGGLIGLDD151 pKa = 3.46 PPVAFPSFKK160 pKa = 10.52 NWVQRR165 pKa = 11.84 DD166 pKa = 3.61 HH167 pKa = 7.16 LSRR170 pKa = 11.84 RR171 pKa = 11.84 LFRR174 pKa = 11.84 EE175 pKa = 3.95 SEE177 pKa = 4.22 VNTRR181 pKa = 11.84 VYY183 pKa = 11.12 KK184 pKa = 10.54 NVYY187 pKa = 10.7 ANFGLKK193 pKa = 10.0 GHH195 pKa = 6.77 IPIDD199 pKa = 3.78 NKK201 pKa = 10.27 VGLYY205 pKa = 9.38 RR206 pKa = 11.84 IRR208 pKa = 11.84 MRR210 pKa = 11.84 CISGGYY216 pKa = 9.35 ARR218 pKa = 11.84 GIFRR222 pKa = 11.84 FTRR225 pKa = 11.84 MSKK228 pKa = 9.02 MAFSQVARR236 pKa = 11.84 EE237 pKa = 4.07 GWLKK241 pKa = 10.78 RR242 pKa = 11.84 FGYY245 pKa = 10.4 RR246 pKa = 11.84 PDD248 pKa = 3.34 LFRR251 pKa = 6.54
Molecular weight: 28.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.853
IPC_protein 10.687
Toseland 10.716
ProMoST 10.555
Dawson 10.833
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 11.052
Grimsley 10.891
Solomon 10.935
Lehninger 10.906
Nozaki 10.701
DTASelect 10.555
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.76
Patrickios 10.73
IPC_peptide 10.935
IPC2_peptide 9.619
IPC2.peptide.svr19 8.451
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5313
0
5313
2605626
22
4800
490.4
55.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.894 ± 0.026
1.862 ± 0.017
6.064 ± 0.02
6.559 ± 0.034
4.289 ± 0.025
5.84 ± 0.031
2.31 ± 0.01
6.765 ± 0.033
7.559 ± 0.033
9.188 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.893 ± 0.012
5.495 ± 0.024
4.093 ± 0.025
2.952 ± 0.016
4.466 ± 0.022
8.575 ± 0.029
5.914 ± 0.022
6.176 ± 0.024
1.091 ± 0.01
4.017 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here