Nocardia terpenica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8083 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164K9H6|A0A164K9H6_9NOCA SARP family transcriptional regulator OS=Nocardia terpenica OX=455432 GN=AWN90_38665 PE=3 SV=1
MM1 pKa = 7.82ADD3 pKa = 3.36LASVYY8 pKa = 10.85DD9 pKa = 4.36EE10 pKa = 4.9AAQDD14 pKa = 3.69TPEE17 pKa = 3.89WAEE20 pKa = 3.43WDD22 pKa = 3.61DD23 pKa = 3.63PRR25 pKa = 11.84RR26 pKa = 11.84SFMQIDD32 pKa = 3.68TLFDD36 pKa = 3.32EE37 pKa = 5.12TLPALPVADD46 pKa = 4.62DD47 pKa = 3.67AALGEE52 pKa = 4.6TVPPRR57 pKa = 11.84PQAPRR62 pKa = 11.84YY63 pKa = 8.89SPAMLEE69 pKa = 4.16WVEE72 pKa = 4.04YY73 pKa = 10.51AAFAMFPTRR82 pKa = 11.84KK83 pKa = 9.79ALADD87 pKa = 3.5NTDD90 pKa = 3.28AADD93 pKa = 3.5QFVCYY98 pKa = 10.28LIEE101 pKa = 4.09YY102 pKa = 9.28LVRR105 pKa = 11.84NAGGLRR111 pKa = 11.84FNVPGNGSPVYY122 pKa = 10.49DD123 pKa = 3.34GFGPSVCYY131 pKa = 10.49NYY133 pKa = 11.06ASDD136 pKa = 3.59VDD138 pKa = 4.16NPVDD142 pKa = 3.42MLLTIGEE149 pKa = 4.38HH150 pKa = 6.59GEE152 pKa = 4.18GFADD156 pKa = 5.44DD157 pKa = 4.64IITRR161 pKa = 11.84AEE163 pKa = 4.38DD164 pKa = 3.68YY165 pKa = 11.26ADD167 pKa = 3.56SATT170 pKa = 3.69

Molecular weight: 18.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A164NWR9|A0A164NWR9_9NOCA Bifunctional 3'-5' exonuclease/DNA polymerase OS=Nocardia terpenica OX=455432 GN=AWN90_22565 PE=4 SV=1
MM1 pKa = 7.82PLLPLGLAAPAGRR14 pKa = 11.84ATGTAEE20 pKa = 4.18GTRR23 pKa = 11.84GTAATAAATRR33 pKa = 11.84TATAATGTTPEE44 pKa = 3.83ATATGRR50 pKa = 11.84GSARR54 pKa = 11.84TGAGTAATAAVVTTAAATGTTAAAGRR80 pKa = 11.84ATRR83 pKa = 11.84TARR86 pKa = 11.84TATRR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84ARR94 pKa = 11.84TRR96 pKa = 11.84RR97 pKa = 11.84ALRR100 pKa = 11.84HH101 pKa = 4.52GTRR104 pKa = 11.84VRR106 pKa = 11.84PRR108 pKa = 11.84GHH110 pKa = 6.83RR111 pKa = 11.84AGARR115 pKa = 11.84PRR117 pKa = 11.84TTRR120 pKa = 11.84TRR122 pKa = 11.84AATLGGRR129 pKa = 11.84RR130 pKa = 11.84TLPARR135 pKa = 11.84GRR137 pKa = 11.84TLSTRR142 pKa = 11.84SRR144 pKa = 11.84ALPARR149 pKa = 11.84GGTRR153 pKa = 11.84AALGRR158 pKa = 11.84TRR160 pKa = 11.84ARR162 pKa = 11.84ARR164 pKa = 11.84LRR166 pKa = 11.84PRR168 pKa = 11.84RR169 pKa = 11.84GTLGRR174 pKa = 11.84GAEE177 pKa = 4.16GVVADD182 pKa = 4.41ARR184 pKa = 11.84GLRR187 pKa = 11.84TRR189 pKa = 11.84LRR191 pKa = 11.84AGPARR196 pKa = 11.84TRR198 pKa = 11.84ALMRR202 pKa = 11.84GSAALVLATGTRR214 pKa = 11.84GARR217 pKa = 11.84SRR219 pKa = 11.84PLVALLLTVVVLLLRR234 pKa = 11.84RR235 pKa = 11.84GRR237 pKa = 11.84GPGVAPDD244 pKa = 3.73AGFADD249 pKa = 3.85GRR251 pKa = 11.84AWLL254 pKa = 4.09

Molecular weight: 25.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8083

0

8083

2507138

37

10051

310.2

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.455 ± 0.046

0.811 ± 0.008

6.236 ± 0.022

5.206 ± 0.027

2.886 ± 0.015

8.851 ± 0.029

2.346 ± 0.013

4.078 ± 0.017

1.8 ± 0.02

10.215 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.864 ± 0.013

1.943 ± 0.015

5.993 ± 0.025

2.811 ± 0.02

8.207 ± 0.033

4.999 ± 0.018

6.215 ± 0.021

8.423 ± 0.026

1.539 ± 0.01

2.122 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski