Linnemannia elongata AG-77
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14849 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A197JCW0|A0A197JCW0_9FUNG Uncharacterized protein OS=Linnemannia elongata AG-77 OX=1314771 GN=K457DRAFT_25561 PE=4 SV=1
MM1 pKa = 7.94 PKK3 pKa = 9.05 TPKK6 pKa = 10.0 RR7 pKa = 11.84 VLTATSDD14 pKa = 3.5 NADD17 pKa = 2.84 ADD19 pKa = 3.94 MQEE22 pKa = 4.32 EE23 pKa = 4.37 EE24 pKa = 4.66 VEE26 pKa = 4.36 GGEE29 pKa = 4.05 PLCEE33 pKa = 3.78 IEE35 pKa = 5.98 KK36 pKa = 10.73 NVLNNVCKK44 pKa = 10.51 AWTAYY49 pKa = 10.53 FGDD52 pKa = 4.22 LAIAPEE58 pKa = 4.28 DD59 pKa = 3.56 EE60 pKa = 4.81 GEE62 pKa = 4.28 DD63 pKa = 3.78 MEE65 pKa = 7.05 GDD67 pKa = 3.54 DD68 pKa = 4.86 YY69 pKa = 11.67 YY70 pKa = 11.83 AEE72 pKa = 4.49 GGYY75 pKa = 10.97 GEE77 pKa = 5.09 DD78 pKa = 3.31 VDD80 pKa = 4.87 MSLSTKK86 pKa = 10.21 GNSGRR91 pKa = 11.84 LDD93 pKa = 4.61 DD94 pKa = 4.1 IDD96 pKa = 4.61 YY97 pKa = 10.83 YY98 pKa = 11.51 CEE100 pKa = 3.72
Molecular weight: 11.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.465
ProMoST 3.795
Dawson 3.656
Bjellqvist 3.859
Wikipedia 3.579
Rodwell 3.503
Grimsley 3.376
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.516
EMBOSS 3.579
Sillero 3.783
Patrickios 0.896
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A197JLX1|A0A197JLX1_9FUNG Uncharacterized protein OS=Linnemannia elongata AG-77 OX=1314771 GN=K457DRAFT_157705 PE=4 SV=1
MM1 pKa = 7.4 SLFHH5 pKa = 6.06 STPVTVQRR13 pKa = 11.84 TYY15 pKa = 10.83 YY16 pKa = 10.03 KK17 pKa = 9.52 PSAMSRR23 pKa = 11.84 LRR25 pKa = 11.84 ALFSSRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 TAVPVATRR41 pKa = 11.84 TTHH44 pKa = 3.84 QRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 PVRR52 pKa = 11.84 QPVRR56 pKa = 11.84 KK57 pKa = 7.89 TGGFGLFRR65 pKa = 11.84 RR66 pKa = 11.84 TPRR69 pKa = 11.84 ARR71 pKa = 11.84 RR72 pKa = 11.84 TVVTPQRR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 GGLFASLRR89 pKa = 11.84 PRR91 pKa = 11.84 RR92 pKa = 11.84 HH93 pKa = 5.45 YY94 pKa = 10.98 GRR96 pKa = 11.84 TRR98 pKa = 11.84 VAPAPRR104 pKa = 11.84 QHH106 pKa = 6.05 HH107 pKa = 5.66 HH108 pKa = 6.44 HH109 pKa = 6.26 GKK111 pKa = 9.93 GRR113 pKa = 11.84 TFATVLAALSLRR125 pKa = 11.84 KK126 pKa = 9.33 HH127 pKa = 5.22 RR128 pKa = 11.84 HH129 pKa = 3.36 RR130 pKa = 11.84 HH131 pKa = 4.11 GAYY134 pKa = 9.23 GGAPMRR140 pKa = 11.84 PRR142 pKa = 11.84 YY143 pKa = 9.55
Molecular weight: 16.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 11.257
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.223
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.93
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14847
2
14849
6795375
49
4727
457.6
50.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.93 ± 0.02
1.252 ± 0.012
5.519 ± 0.016
5.814 ± 0.022
3.658 ± 0.013
6.457 ± 0.021
2.703 ± 0.013
4.886 ± 0.015
4.982 ± 0.02
8.926 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.009
3.997 ± 0.013
5.759 ± 0.023
4.738 ± 0.027
5.51 ± 0.015
9.02 ± 0.034
6.5 ± 0.018
6.043 ± 0.018
1.196 ± 0.007
2.757 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here