Peptostreptococcus russellii
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1869 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H8ESF9|A0A1H8ESF9_9FIRM Glyceraldehyde-3-phosphate dehydrogenase OS=Peptostreptococcus russellii OX=215200 GN=SAMN05216454_101208 PE=3 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.5 KK3 pKa = 10.33 LLLGAALGIAAGAVGYY19 pKa = 10.38 KK20 pKa = 10.14 LYY22 pKa = 10.85 KK23 pKa = 10.32 EE24 pKa = 4.17 NEE26 pKa = 3.91 EE27 pKa = 4.23 EE28 pKa = 3.87 IKK30 pKa = 11.15 GFIDD34 pKa = 4.48 EE35 pKa = 4.82 RR36 pKa = 11.84 LSDD39 pKa = 3.93 YY40 pKa = 11.6 DD41 pKa = 4.19 DD42 pKa = 4.48 VDD44 pKa = 4.06 VEE46 pKa = 5.77 DD47 pKa = 5.41 IDD49 pKa = 6.24 LEE51 pKa = 4.26 DD52 pKa = 5.69 LEE54 pKa = 5.32 DD55 pKa = 5.35 LRR57 pKa = 11.84 DD58 pKa = 4.79 CIDD61 pKa = 4.09 DD62 pKa = 4.49 MIDD65 pKa = 3.14 AKK67 pKa = 11.11 LKK69 pKa = 11.08 GGFQYY74 pKa = 11.5 DD75 pKa = 4.75 DD76 pKa = 3.8 ISSCDD81 pKa = 5.52 DD82 pKa = 3.86 EE83 pKa = 5.81 DD84 pKa = 4.46 CCCSDD89 pKa = 3.81 DD90 pKa = 4.72 CSCEE94 pKa = 3.87 EE95 pKa = 4.27 EE96 pKa = 4.7 KK97 pKa = 11.15 NYY99 pKa = 10.89 DD100 pKa = 3.57 DD101 pKa = 6.17 DD102 pKa = 5.0 YY103 pKa = 11.73 ICIVHH108 pKa = 6.36 NSEE111 pKa = 4.12 EE112 pKa = 4.19 EE113 pKa = 4.34 KK114 pKa = 10.83 EE115 pKa = 4.02 DD116 pKa = 4.54 SEE118 pKa = 5.27 DD119 pKa = 3.71 NKK121 pKa = 11.05 EE122 pKa = 4.02 KK123 pKa = 11.03
Molecular weight: 14.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.872
Patrickios 1.863
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A1H8KEJ2|A0A1H8KEJ2_9FIRM 2-iminoacetate synthase OS=Peptostreptococcus russellii OX=215200 GN=SAMN05216454_12913 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 8.57 KK9 pKa = 7.61 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.25 RR14 pKa = 11.84 EE15 pKa = 3.66 HH16 pKa = 6.29 GFRR19 pKa = 11.84 KK20 pKa = 9.77 RR21 pKa = 11.84 MKK23 pKa = 9.11 TSNGRR28 pKa = 11.84 NVLKK32 pKa = 10.51 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.37 GRR39 pKa = 11.84 KK40 pKa = 8.95 RR41 pKa = 11.84 LTHH44 pKa = 6.22
Molecular weight: 5.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.003
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1869
0
1869
597693
26
2109
319.8
36.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.034 ± 0.054
1.088 ± 0.02
6.059 ± 0.043
8.064 ± 0.073
4.281 ± 0.048
6.447 ± 0.047
1.307 ± 0.022
9.373 ± 0.062
9.346 ± 0.069
8.489 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.894 ± 0.028
5.679 ± 0.05
2.726 ± 0.026
2.127 ± 0.024
3.722 ± 0.033
6.441 ± 0.05
4.603 ± 0.04
6.728 ± 0.044
0.598 ± 0.019
3.994 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here