Beihai narna-like virus 6
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI33|A0A1L3KI33_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 6 OX=1922458 PE=4 SV=1
MM1 pKa = 7.5 FCRR4 pKa = 11.84 SVWDD8 pKa = 3.84 VLCDD12 pKa = 3.46 ADD14 pKa = 4.27 QVFRR18 pKa = 11.84 YY19 pKa = 8.85 MPARR23 pKa = 11.84 CFLDD27 pKa = 3.94 GFLPLLMRR35 pKa = 11.84 AKK37 pKa = 10.74 DD38 pKa = 3.38 EE39 pKa = 4.35 AEE41 pKa = 3.77 QVKK44 pKa = 7.72 WQKK47 pKa = 11.29 YY48 pKa = 5.12 LTCWPMAKK56 pKa = 10.15 FLRR59 pKa = 11.84 QTSMPEE65 pKa = 3.73 VPAGLEE71 pKa = 3.89 EE72 pKa = 5.28 AIGSSFHH79 pKa = 7.15 FPLTGAARR87 pKa = 11.84 RR88 pKa = 11.84 HH89 pKa = 5.45 LRR91 pKa = 11.84 NLLASRR97 pKa = 11.84 DD98 pKa = 3.74 SEE100 pKa = 3.89 IRR102 pKa = 11.84 PTQVCWAILQGVKK115 pKa = 10.22 RR116 pKa = 11.84 GCAEE120 pKa = 3.69 VSEE123 pKa = 4.43 DD124 pKa = 4.46 FIYY127 pKa = 9.48 ATMLKK132 pKa = 9.83 HH133 pKa = 6.44 KK134 pKa = 10.7 ASLTQTLPEE143 pKa = 3.99 MSEE146 pKa = 3.95 EE147 pKa = 3.84 ATEE150 pKa = 4.1 LVRR153 pKa = 11.84 QKK155 pKa = 10.32 FRR157 pKa = 11.84 NIWRR161 pKa = 11.84 PSFRR165 pKa = 11.84 RR166 pKa = 11.84 RR167 pKa = 11.84 QWTGYY172 pKa = 7.29 GTSRR176 pKa = 11.84 QMEE179 pKa = 4.37 RR180 pKa = 11.84 DD181 pKa = 3.58 CSISGNPNFNACYY194 pKa = 9.78 EE195 pKa = 4.35 VTRR198 pKa = 11.84 SQGGRR203 pKa = 11.84 CRR205 pKa = 11.84 AVRR208 pKa = 11.84 STIIRR213 pKa = 11.84 YY214 pKa = 8.65 LRR216 pKa = 11.84 EE217 pKa = 3.78 EE218 pKa = 3.78 WGMDD222 pKa = 2.97 SDD224 pKa = 4.56 NRR226 pKa = 11.84 LLYY229 pKa = 10.8 RR230 pKa = 11.84 MTEE233 pKa = 3.98 VAPGEE238 pKa = 4.38 VVSEE242 pKa = 4.21 YY243 pKa = 10.23 IGPEE247 pKa = 3.75 FLPEE251 pKa = 4.2 VSQKK255 pKa = 10.62 LAEE258 pKa = 4.02 RR259 pKa = 11.84 WALKK263 pKa = 10.44 DD264 pKa = 3.54 LEE266 pKa = 4.5 RR267 pKa = 11.84 LGGTCRR273 pKa = 11.84 ATVCAILEE281 pKa = 4.05 PLKK284 pKa = 10.95 CRR286 pKa = 11.84 LITKK290 pKa = 10.02 GSALPYY296 pKa = 9.82 FAAQALQKK304 pKa = 11.23 AMWQRR309 pKa = 11.84 LQAFPCFKK317 pKa = 10.41 LTGCPLDD324 pKa = 4.27 ASMLQGLLDD333 pKa = 3.5 QEE335 pKa = 4.39 EE336 pKa = 4.28 RR337 pKa = 11.84 LGLDD341 pKa = 3.14 FTHH344 pKa = 6.99 WVSGDD349 pKa = 3.32 YY350 pKa = 10.73 SAATDD355 pKa = 3.92 GLSQQVNRR363 pKa = 11.84 LCLEE367 pKa = 3.9 EE368 pKa = 4.7 ALRR371 pKa = 11.84 GCNASEE377 pKa = 3.81 ALKK380 pKa = 10.5 IIARR384 pKa = 11.84 AVLGNHH390 pKa = 6.64 RR391 pKa = 11.84 IEE393 pKa = 4.44 YY394 pKa = 6.88 PTEE397 pKa = 3.9 FEE399 pKa = 4.25 IDD401 pKa = 3.79 GMMQQNGQLMGSPLSFPVLCSINVAAYY428 pKa = 7.28 WCALEE433 pKa = 5.16 EE434 pKa = 4.0 YY435 pKa = 9.38 TGRR438 pKa = 11.84 KK439 pKa = 8.19 FQLEE443 pKa = 4.24 DD444 pKa = 3.98 LPCLVNGDD452 pKa = 4.86 DD453 pKa = 3.24 ICFRR457 pKa = 11.84 ANPEE461 pKa = 4.4 FYY463 pKa = 10.7 GIWQRR468 pKa = 11.84 WVRR471 pKa = 11.84 EE472 pKa = 3.95 VGFTLSPGKK481 pKa = 10.4 NYY483 pKa = 9.68 IHH485 pKa = 6.16 EE486 pKa = 4.53 HH487 pKa = 5.02 FVTVNSEE494 pKa = 4.46 GYY496 pKa = 9.0 VHH498 pKa = 6.36 QKK500 pKa = 9.18 HH501 pKa = 5.95 GKK503 pKa = 9.21 VPFHH507 pKa = 6.71 KK508 pKa = 10.96 VNFLNTGLLYY518 pKa = 10.57 SGKK521 pKa = 9.92 SVEE524 pKa = 5.53 RR525 pKa = 11.84 KK526 pKa = 9.35 IDD528 pKa = 3.31 WGDD531 pKa = 3.34 QPAIKK536 pKa = 10.47 VGLRR540 pKa = 11.84 PEE542 pKa = 3.97 NRR544 pKa = 11.84 EE545 pKa = 3.62 MPFTQKK551 pKa = 9.85 VNRR554 pKa = 11.84 VIEE557 pKa = 4.48 EE558 pKa = 4.22 SCSPTRR564 pKa = 11.84 TLLRR568 pKa = 11.84 VHH570 pKa = 7.29 RR571 pKa = 11.84 YY572 pKa = 8.43 FRR574 pKa = 11.84 DD575 pKa = 3.47 EE576 pKa = 4.1 IKK578 pKa = 10.77 YY579 pKa = 8.06 HH580 pKa = 5.13 TLNGEE585 pKa = 4.42 INMHH589 pKa = 6.9 AAPEE593 pKa = 4.15 LGGLGIVLPEE603 pKa = 4.37 GSDD606 pKa = 3.31 TRR608 pKa = 11.84 FTAWQCRR615 pKa = 11.84 AAGYY619 pKa = 9.02 LASRR623 pKa = 11.84 WKK625 pKa = 10.44 SLEE628 pKa = 3.82 FGSVVEE634 pKa = 5.23 GIPGPLTQEE643 pKa = 4.08 EE644 pKa = 4.34 AKK646 pKa = 10.71 HH647 pKa = 6.44 RR648 pKa = 11.84 IVDD651 pKa = 4.05 LNRR654 pKa = 11.84 PLGVEE659 pKa = 3.95 GRR661 pKa = 11.84 VTYY664 pKa = 7.93 QQKK667 pKa = 10.55 KK668 pKa = 8.98 SAALTWQAPVKK679 pKa = 9.44 PGRR682 pKa = 11.84 VVVRR686 pKa = 11.84 EE687 pKa = 3.76 KK688 pKa = 11.22 LEE690 pKa = 3.79 PLRR693 pKa = 11.84 EE694 pKa = 4.16 GEE696 pKa = 4.12 EE697 pKa = 4.12 RR698 pKa = 11.84 IEE700 pKa = 4.42 PDD702 pKa = 2.96 SSPLRR707 pKa = 11.84 NYY709 pKa = 9.9 QGPPTSANGEE719 pKa = 4.17 WKK721 pKa = 10.35 IKK723 pKa = 10.77 GLDD726 pKa = 3.61 SEE728 pKa = 4.57 CRR730 pKa = 11.84 QAIRR734 pKa = 11.84 EE735 pKa = 4.02 YY736 pKa = 10.47 RR737 pKa = 11.84 GSGVNRR743 pKa = 11.84 PLRR746 pKa = 11.84 WSRR749 pKa = 11.84 EE750 pKa = 3.84 VRR752 pKa = 11.84 SAPSSILDD760 pKa = 3.3 RR761 pKa = 11.84 LVRR764 pKa = 11.84 SDD766 pKa = 3.47 STSVLNTWIGAEE778 pKa = 4.18 GQVHH782 pKa = 6.28 SFLSKK787 pKa = 10.23 PVLVGG792 pKa = 3.35
Molecular weight: 90.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.288
IPC2_protein 7.629
IPC_protein 7.41
Toseland 6.971
ProMoST 8.039
Dawson 8.126
Bjellqvist 8.624
Wikipedia 8.039
Rodwell 8.156
Grimsley 7.0
Solomon 8.156
Lehninger 8.185
Nozaki 8.873
DTASelect 8.273
Thurlkill 8.331
EMBOSS 8.331
Sillero 8.697
Patrickios 4.406
IPC_peptide 8.156
IPC2_peptide 7.732
IPC2.peptide.svr19 7.718
Protein with the highest isoelectric point:
>tr|A0A1L3KI33|A0A1L3KI33_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 6 OX=1922458 PE=4 SV=1
MM1 pKa = 7.5 FCRR4 pKa = 11.84 SVWDD8 pKa = 3.84 VLCDD12 pKa = 3.46 ADD14 pKa = 4.27 QVFRR18 pKa = 11.84 YY19 pKa = 8.85 MPARR23 pKa = 11.84 CFLDD27 pKa = 3.94 GFLPLLMRR35 pKa = 11.84 AKK37 pKa = 10.74 DD38 pKa = 3.38 EE39 pKa = 4.35 AEE41 pKa = 3.77 QVKK44 pKa = 7.72 WQKK47 pKa = 11.29 YY48 pKa = 5.12 LTCWPMAKK56 pKa = 10.15 FLRR59 pKa = 11.84 QTSMPEE65 pKa = 3.73 VPAGLEE71 pKa = 3.89 EE72 pKa = 5.28 AIGSSFHH79 pKa = 7.15 FPLTGAARR87 pKa = 11.84 RR88 pKa = 11.84 HH89 pKa = 5.45 LRR91 pKa = 11.84 NLLASRR97 pKa = 11.84 DD98 pKa = 3.74 SEE100 pKa = 3.89 IRR102 pKa = 11.84 PTQVCWAILQGVKK115 pKa = 10.22 RR116 pKa = 11.84 GCAEE120 pKa = 3.69 VSEE123 pKa = 4.43 DD124 pKa = 4.46 FIYY127 pKa = 9.48 ATMLKK132 pKa = 9.83 HH133 pKa = 6.44 KK134 pKa = 10.7 ASLTQTLPEE143 pKa = 3.99 MSEE146 pKa = 3.95 EE147 pKa = 3.84 ATEE150 pKa = 4.1 LVRR153 pKa = 11.84 QKK155 pKa = 10.32 FRR157 pKa = 11.84 NIWRR161 pKa = 11.84 PSFRR165 pKa = 11.84 RR166 pKa = 11.84 RR167 pKa = 11.84 QWTGYY172 pKa = 7.29 GTSRR176 pKa = 11.84 QMEE179 pKa = 4.37 RR180 pKa = 11.84 DD181 pKa = 3.58 CSISGNPNFNACYY194 pKa = 9.78 EE195 pKa = 4.35 VTRR198 pKa = 11.84 SQGGRR203 pKa = 11.84 CRR205 pKa = 11.84 AVRR208 pKa = 11.84 STIIRR213 pKa = 11.84 YY214 pKa = 8.65 LRR216 pKa = 11.84 EE217 pKa = 3.78 EE218 pKa = 3.78 WGMDD222 pKa = 2.97 SDD224 pKa = 4.56 NRR226 pKa = 11.84 LLYY229 pKa = 10.8 RR230 pKa = 11.84 MTEE233 pKa = 3.98 VAPGEE238 pKa = 4.38 VVSEE242 pKa = 4.21 YY243 pKa = 10.23 IGPEE247 pKa = 3.75 FLPEE251 pKa = 4.2 VSQKK255 pKa = 10.62 LAEE258 pKa = 4.02 RR259 pKa = 11.84 WALKK263 pKa = 10.44 DD264 pKa = 3.54 LEE266 pKa = 4.5 RR267 pKa = 11.84 LGGTCRR273 pKa = 11.84 ATVCAILEE281 pKa = 4.05 PLKK284 pKa = 10.95 CRR286 pKa = 11.84 LITKK290 pKa = 10.02 GSALPYY296 pKa = 9.82 FAAQALQKK304 pKa = 11.23 AMWQRR309 pKa = 11.84 LQAFPCFKK317 pKa = 10.41 LTGCPLDD324 pKa = 4.27 ASMLQGLLDD333 pKa = 3.5 QEE335 pKa = 4.39 EE336 pKa = 4.28 RR337 pKa = 11.84 LGLDD341 pKa = 3.14 FTHH344 pKa = 6.99 WVSGDD349 pKa = 3.32 YY350 pKa = 10.73 SAATDD355 pKa = 3.92 GLSQQVNRR363 pKa = 11.84 LCLEE367 pKa = 3.9 EE368 pKa = 4.7 ALRR371 pKa = 11.84 GCNASEE377 pKa = 3.81 ALKK380 pKa = 10.5 IIARR384 pKa = 11.84 AVLGNHH390 pKa = 6.64 RR391 pKa = 11.84 IEE393 pKa = 4.44 YY394 pKa = 6.88 PTEE397 pKa = 3.9 FEE399 pKa = 4.25 IDD401 pKa = 3.79 GMMQQNGQLMGSPLSFPVLCSINVAAYY428 pKa = 7.28 WCALEE433 pKa = 5.16 EE434 pKa = 4.0 YY435 pKa = 9.38 TGRR438 pKa = 11.84 KK439 pKa = 8.19 FQLEE443 pKa = 4.24 DD444 pKa = 3.98 LPCLVNGDD452 pKa = 4.86 DD453 pKa = 3.24 ICFRR457 pKa = 11.84 ANPEE461 pKa = 4.4 FYY463 pKa = 10.7 GIWQRR468 pKa = 11.84 WVRR471 pKa = 11.84 EE472 pKa = 3.95 VGFTLSPGKK481 pKa = 10.4 NYY483 pKa = 9.68 IHH485 pKa = 6.16 EE486 pKa = 4.53 HH487 pKa = 5.02 FVTVNSEE494 pKa = 4.46 GYY496 pKa = 9.0 VHH498 pKa = 6.36 QKK500 pKa = 9.18 HH501 pKa = 5.95 GKK503 pKa = 9.21 VPFHH507 pKa = 6.71 KK508 pKa = 10.96 VNFLNTGLLYY518 pKa = 10.57 SGKK521 pKa = 9.92 SVEE524 pKa = 5.53 RR525 pKa = 11.84 KK526 pKa = 9.35 IDD528 pKa = 3.31 WGDD531 pKa = 3.34 QPAIKK536 pKa = 10.47 VGLRR540 pKa = 11.84 PEE542 pKa = 3.97 NRR544 pKa = 11.84 EE545 pKa = 3.62 MPFTQKK551 pKa = 9.85 VNRR554 pKa = 11.84 VIEE557 pKa = 4.48 EE558 pKa = 4.22 SCSPTRR564 pKa = 11.84 TLLRR568 pKa = 11.84 VHH570 pKa = 7.29 RR571 pKa = 11.84 YY572 pKa = 8.43 FRR574 pKa = 11.84 DD575 pKa = 3.47 EE576 pKa = 4.1 IKK578 pKa = 10.77 YY579 pKa = 8.06 HH580 pKa = 5.13 TLNGEE585 pKa = 4.42 INMHH589 pKa = 6.9 AAPEE593 pKa = 4.15 LGGLGIVLPEE603 pKa = 4.37 GSDD606 pKa = 3.31 TRR608 pKa = 11.84 FTAWQCRR615 pKa = 11.84 AAGYY619 pKa = 9.02 LASRR623 pKa = 11.84 WKK625 pKa = 10.44 SLEE628 pKa = 3.82 FGSVVEE634 pKa = 5.23 GIPGPLTQEE643 pKa = 4.08 EE644 pKa = 4.34 AKK646 pKa = 10.71 HH647 pKa = 6.44 RR648 pKa = 11.84 IVDD651 pKa = 4.05 LNRR654 pKa = 11.84 PLGVEE659 pKa = 3.95 GRR661 pKa = 11.84 VTYY664 pKa = 7.93 QQKK667 pKa = 10.55 KK668 pKa = 8.98 SAALTWQAPVKK679 pKa = 9.44 PGRR682 pKa = 11.84 VVVRR686 pKa = 11.84 EE687 pKa = 3.76 KK688 pKa = 11.22 LEE690 pKa = 3.79 PLRR693 pKa = 11.84 EE694 pKa = 4.16 GEE696 pKa = 4.12 EE697 pKa = 4.12 RR698 pKa = 11.84 IEE700 pKa = 4.42 PDD702 pKa = 2.96 SSPLRR707 pKa = 11.84 NYY709 pKa = 9.9 QGPPTSANGEE719 pKa = 4.17 WKK721 pKa = 10.35 IKK723 pKa = 10.77 GLDD726 pKa = 3.61 SEE728 pKa = 4.57 CRR730 pKa = 11.84 QAIRR734 pKa = 11.84 EE735 pKa = 4.02 YY736 pKa = 10.47 RR737 pKa = 11.84 GSGVNRR743 pKa = 11.84 PLRR746 pKa = 11.84 WSRR749 pKa = 11.84 EE750 pKa = 3.84 VRR752 pKa = 11.84 SAPSSILDD760 pKa = 3.3 RR761 pKa = 11.84 LVRR764 pKa = 11.84 SDD766 pKa = 3.47 STSVLNTWIGAEE778 pKa = 4.18 GQVHH782 pKa = 6.28 SFLSKK787 pKa = 10.23 PVLVGG792 pKa = 3.35
Molecular weight: 90.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.288
IPC2_protein 7.629
IPC_protein 7.41
Toseland 6.971
ProMoST 8.039
Dawson 8.126
Bjellqvist 8.624
Wikipedia 8.039
Rodwell 8.156
Grimsley 7.0
Solomon 8.156
Lehninger 8.185
Nozaki 8.873
DTASelect 8.273
Thurlkill 8.331
EMBOSS 8.331
Sillero 8.697
Patrickios 4.406
IPC_peptide 8.156
IPC2_peptide 7.732
IPC2.peptide.svr19 7.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
792
792
792
792.0
90.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.944 ± 0.0
2.904 ± 0.0
3.662 ± 0.0
8.081 ± 0.0
3.788 ± 0.0
7.449 ± 0.0
1.894 ± 0.0
4.04 ± 0.0
4.293 ± 0.0
9.848 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.146 ± 0.0
3.283 ± 0.0
5.429 ± 0.0
4.545 ± 0.0
8.712 ± 0.0
6.566 ± 0.0
4.545 ± 0.0
6.439 ± 0.0
2.525 ± 0.0
2.904 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here