Streptococcus phage IPP69

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SGG7|A0A1S5SGG7_9CAUD Terminase small subunit OS=Streptococcus phage IPP69 OX=1916205 GN=IPP69_00041 PE=4 SV=1
MM1 pKa = 7.54TKK3 pKa = 10.04TIEE6 pKa = 4.2LPDD9 pKa = 3.86YY10 pKa = 11.11YY11 pKa = 10.99EE12 pKa = 4.72PDD14 pKa = 3.19WGNARR19 pKa = 11.84YY20 pKa = 7.53GTLEE24 pKa = 3.87EE25 pKa = 4.36LKK27 pKa = 10.87EE28 pKa = 3.71LLLYY32 pKa = 10.33KK33 pKa = 10.48RR34 pKa = 11.84IVEE37 pKa = 3.91WDD39 pKa = 3.14KK40 pKa = 11.83DD41 pKa = 3.83FLLLEE46 pKa = 5.42DD47 pKa = 3.96GTKK50 pKa = 8.98VTIEE54 pKa = 4.02MSEE57 pKa = 4.16SDD59 pKa = 4.01CCASAGGEE67 pKa = 4.09FQNVSLDD74 pKa = 3.68AVITNVEE81 pKa = 3.69IGEE84 pKa = 4.07QEE86 pKa = 4.68EE87 pKa = 5.28IPDD90 pKa = 3.39HH91 pKa = 6.31WGAGYY96 pKa = 10.19KK97 pKa = 10.47NKK99 pKa = 8.57VTIFHH104 pKa = 6.23NQNPVAIANCEE115 pKa = 4.04AEE117 pKa = 4.32HH118 pKa = 6.09NGYY121 pKa = 9.85YY122 pKa = 10.37YY123 pKa = 10.92SVGSLVIGDD132 pKa = 2.94IHH134 pKa = 6.99FPVVHH139 pKa = 6.79AA140 pKa = 5.01

Molecular weight: 15.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SGH7|A0A1S5SGH7_9CAUD Uncharacterized protein OS=Streptococcus phage IPP69 OX=1916205 GN=IPP69_00062 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.36PYY8 pKa = 9.5SGKK11 pKa = 9.72RR12 pKa = 11.84KK13 pKa = 9.85KK14 pKa = 10.46PIGQSIDD21 pKa = 3.05LVARR25 pKa = 11.84LFRR28 pKa = 11.84LEE30 pKa = 4.04SQVISLANHH39 pKa = 6.11EE40 pKa = 4.4MFKK43 pKa = 10.04MPSSRR48 pKa = 11.84SSTVV52 pKa = 2.51

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12809

35

2417

194.1

22.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.722 ± 0.539

0.648 ± 0.097

6.246 ± 0.273

8.393 ± 0.472

3.997 ± 0.245

6.277 ± 0.473

1.304 ± 0.146

6.581 ± 0.24

8.471 ± 0.331

8.447 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.247

5.254 ± 0.278

2.732 ± 0.219

4.06 ± 0.221

4.895 ± 0.315

6.152 ± 0.394

5.941 ± 0.453

6.066 ± 0.231

1.429 ± 0.151

3.896 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski