filamentous cyanobacterium CCP2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; unclassified Cyanobacteria

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T1D2N7|A0A2T1D2N7_9CYAN Thiazole synthase (Fragment) OS=filamentous cyanobacterium CCP2 OX=2107700 GN=C7B76_15640 PE=3 SV=1
MM1 pKa = 7.76PGNLRR6 pKa = 11.84ALKK9 pKa = 8.79EE10 pKa = 4.39TIATLQDD17 pKa = 3.22RR18 pKa = 11.84LHH20 pKa = 6.93PIVLPIYY27 pKa = 10.13CYY29 pKa = 10.17TEE31 pKa = 3.45ISKK34 pKa = 10.29VDD36 pKa = 3.59EE37 pKa = 4.53FLTPQLFTPGSDD49 pKa = 2.51IAIGFRR55 pKa = 11.84PSQIVWGRR63 pKa = 11.84TGNDD67 pKa = 2.91AVLGYY72 pKa = 9.53QPPASQTVQPQLDD85 pKa = 3.76ILIGDD90 pKa = 4.81LAIEE94 pKa = 4.72DD95 pKa = 3.7PAFRR99 pKa = 11.84QWSDD103 pKa = 3.09TFILGDD109 pKa = 3.39WTRR112 pKa = 11.84PYY114 pKa = 10.92YY115 pKa = 9.92IDD117 pKa = 4.75PEE119 pKa = 4.51PGFLGLNNFAILADD133 pKa = 4.55FDD135 pKa = 4.24PTLDD139 pKa = 3.92TIQLFGSANDD149 pKa = 3.74YY150 pKa = 11.07QLLDD154 pKa = 4.07LGFGTGIAFQTSTGPDD170 pKa = 3.57LIGFALGASGLNLAEE185 pKa = 5.44DD186 pKa = 4.02YY187 pKa = 11.41FEE189 pKa = 5.36FRR191 pKa = 11.84GTTPPPGPEE200 pKa = 3.77VPQIQQFGTTEE211 pKa = 3.64FDD213 pKa = 3.14IPLSISSDD221 pKa = 3.39PFGNIYY227 pKa = 10.06VAGGTNASLIEE238 pKa = 4.27TNEE241 pKa = 3.73GLRR244 pKa = 11.84DD245 pKa = 3.9NFVTKK250 pKa = 10.74YY251 pKa = 10.47NSQGQEE257 pKa = 3.88LFSLQFGTEE266 pKa = 3.96GFEE269 pKa = 4.2TIYY272 pKa = 11.1GIDD275 pKa = 3.71TDD277 pKa = 4.58DD278 pKa = 4.82LGNFYY283 pKa = 9.33ITGVTDD289 pKa = 4.47GEE291 pKa = 4.41LASPRR296 pKa = 11.84QSALLDD302 pKa = 3.67TFVAKK307 pKa = 10.72YY308 pKa = 10.29DD309 pKa = 4.08GEE311 pKa = 5.41GNQQWIRR318 pKa = 11.84QIGEE322 pKa = 3.87NLIFNAFNIAVDD334 pKa = 4.33GPTGDD339 pKa = 3.73VFISGANVKK348 pKa = 9.9PDD350 pKa = 3.86LANPDD355 pKa = 3.59DD356 pKa = 4.37AFVIKK361 pKa = 10.42FDD363 pKa = 4.31TDD365 pKa = 4.16GNQQWFTEE373 pKa = 4.19TGTTGFLNFDD383 pKa = 3.45EE384 pKa = 5.18TYY386 pKa = 10.93GLTVANDD393 pKa = 3.65GSVYY397 pKa = 9.49ATGWTVGDD405 pKa = 4.07LGGPNQGLYY414 pKa = 10.89DD415 pKa = 3.71NWIAKK420 pKa = 9.51YY421 pKa = 10.93DD422 pKa = 3.68NTTGEE427 pKa = 4.57EE428 pKa = 4.28VWVVQYY434 pKa = 7.19GTPDD438 pKa = 3.8YY439 pKa = 10.2EE440 pKa = 4.24WSWDD444 pKa = 3.46VRR446 pKa = 11.84TDD448 pKa = 3.37SLGNVYY454 pKa = 8.59TAGWTLGTLGDD465 pKa = 3.86EE466 pKa = 4.64SFGSFDD472 pKa = 4.81AYY474 pKa = 9.03LTKK477 pKa = 10.45YY478 pKa = 10.78DD479 pKa = 4.27SDD481 pKa = 4.69GNPLWVQQFGTPEE494 pKa = 4.56DD495 pKa = 3.8DD496 pKa = 3.13QAYY499 pKa = 10.87SLFIDD504 pKa = 3.27PHH506 pKa = 8.02DD507 pKa = 4.59NIFVGGYY514 pKa = 4.91TQGDD518 pKa = 3.55FGGQNVGSFDD528 pKa = 2.93AWIAKK533 pKa = 9.51FNTEE537 pKa = 4.99GEE539 pKa = 4.6KK540 pKa = 10.02IWINQFGTPNRR551 pKa = 11.84DD552 pKa = 2.97EE553 pKa = 5.52LYY555 pKa = 11.26GLTADD560 pKa = 4.38NAGNLYY566 pKa = 9.43ATGITQGSLGEE577 pKa = 4.2LNAGSFDD584 pKa = 3.32GWTAKK589 pKa = 10.65LDD591 pKa = 3.72VATGQLLDD599 pKa = 4.11FSGQAIDD606 pKa = 4.66APVEE610 pKa = 4.07EE611 pKa = 5.41DD612 pKa = 3.94CDD614 pKa = 4.27PLTGMPINSEE624 pKa = 4.14DD625 pKa = 3.6AVHH628 pKa = 7.14PEE630 pKa = 3.78AEE632 pKa = 4.28QPQLSNVEE640 pKa = 3.82IAQANGMSTALKK652 pKa = 10.46DD653 pKa = 3.58STFTPDD659 pKa = 2.77ILTGGSEE666 pKa = 4.07PPIEE670 pKa = 4.87EE671 pKa = 3.83IANPSGIDD679 pKa = 3.21LGAFFAAGSNPYY691 pKa = 9.63QAVSIQSLFANAFGLEE707 pKa = 3.85GVMDD711 pKa = 4.4NMPSYY716 pKa = 10.56QAVSSSSAFSQSS728 pKa = 2.59

Molecular weight: 78.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T1CJJ5|A0A2T1CJJ5_9CYAN Cobalt transport protein CbiN OS=filamentous cyanobacterium CCP2 OX=2107700 GN=cbiN PE=3 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 9.07RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 9.95NGRR29 pKa = 11.84RR30 pKa = 11.84VIKK33 pKa = 10.23ARR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84GRR40 pKa = 11.84VRR42 pKa = 11.84LAVV45 pKa = 3.37

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6213

0

6213

1872383

27

2476

301.4

33.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.769 ± 0.034

0.965 ± 0.011

5.166 ± 0.023

6.33 ± 0.033

3.911 ± 0.02

6.849 ± 0.036

2.088 ± 0.018

6.029 ± 0.025

3.439 ± 0.03

11.081 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.014

3.615 ± 0.023

5.111 ± 0.025

5.594 ± 0.031

5.816 ± 0.027

6.344 ± 0.025

5.658 ± 0.026

6.875 ± 0.026

1.512 ± 0.015

2.844 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski