Acinetobacter phage AbTJ
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410T650|A0A410T650_9CAUD Uncharacterized protein OS=Acinetobacter phage AbTJ OX=2507835 PE=4 SV=1
MM1 pKa = 7.74 SEE3 pKa = 4.27 VKK5 pKa = 10.74 VKK7 pKa = 9.63 TCDD10 pKa = 3.31 FCDD13 pKa = 4.32 DD14 pKa = 4.35 GNGEE18 pKa = 5.06 CIFPYY23 pKa = 10.42 YY24 pKa = 10.77 GLAPHH29 pKa = 7.15 IHH31 pKa = 6.21 TKK33 pKa = 10.42 PIGGTVFLDD42 pKa = 4.09 GSLPEE47 pKa = 4.31 NFCPDD52 pKa = 3.31 GDD54 pKa = 4.17 GLGMYY59 pKa = 7.35 THH61 pKa = 7.58 CLNCGGDD68 pKa = 3.57 GTYY71 pKa = 10.62 EE72 pKa = 4.09 GTQLEE77 pKa = 4.69 VKK79 pKa = 10.65 AEE81 pKa = 4.25 SKK83 pKa = 11.1 EE84 pKa = 4.07 EE85 pKa = 3.86
Molecular weight: 9.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.168
IPC2_protein 4.329
IPC_protein 4.215
Toseland 4.05
ProMoST 4.279
Dawson 4.177
Bjellqvist 4.406
Wikipedia 4.075
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.126
Nozaki 4.304
DTASelect 4.457
Thurlkill 4.075
EMBOSS 4.088
Sillero 4.342
Patrickios 0.35
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.302
Protein with the highest isoelectric point:
>tr|A0A410T5T9|A0A410T5T9_9CAUD Uncharacterized protein OS=Acinetobacter phage AbTJ OX=2507835 PE=4 SV=1
MM1 pKa = 7.39 SSVSIAEE8 pKa = 3.85 YY9 pKa = 10.59 RR10 pKa = 11.84 KK11 pKa = 10.26 LFPIKK16 pKa = 10.04 KK17 pKa = 7.86 NKK19 pKa = 8.92 KK20 pKa = 8.71 RR21 pKa = 11.84 RR22 pKa = 11.84 SAKK25 pKa = 9.41 QVARR29 pKa = 11.84 QPSVGEE35 pKa = 4.17 VVLATHH41 pKa = 7.24 LRR43 pKa = 11.84 ACKK46 pKa = 9.84 IGFEE50 pKa = 4.22 QEE52 pKa = 4.33 YY53 pKa = 10.13 KK54 pKa = 10.5 FHH56 pKa = 7.64 PEE58 pKa = 3.89 RR59 pKa = 11.84 KK60 pKa = 8.02 WRR62 pKa = 11.84 ADD64 pKa = 3.43 FLIKK68 pKa = 10.32 GSKK71 pKa = 9.64 ILIEE75 pKa = 4.15 VEE77 pKa = 3.78 GGIWSGGRR85 pKa = 11.84 HH86 pKa = 4.61 TRR88 pKa = 11.84 GKK90 pKa = 10.85 GYY92 pKa = 10.3 LGDD95 pKa = 3.65 MEE97 pKa = 5.25 KK98 pKa = 10.99 YY99 pKa = 10.5 NSAAMMGFTVLRR111 pKa = 11.84 FSTEE115 pKa = 3.74 QVKK118 pKa = 10.86 AGVAIKK124 pKa = 10.35 QIEE127 pKa = 4.16 QLVGG131 pKa = 3.16
Molecular weight: 14.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.153
IPC2_protein 9.472
IPC_protein 9.502
Toseland 10.423
ProMoST 9.94
Dawson 10.526
Bjellqvist 10.131
Wikipedia 10.643
Rodwell 11.111
Grimsley 10.57
Solomon 10.555
Lehninger 10.54
Nozaki 10.394
DTASelect 10.131
Thurlkill 10.409
EMBOSS 10.804
Sillero 10.438
Patrickios 10.847
IPC_peptide 10.57
IPC2_peptide 8.624
IPC2.peptide.svr19 8.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
13243
49
699
213.6
23.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.317 ± 0.356
1.057 ± 0.113
6.018 ± 0.251
6.6 ± 0.372
4.017 ± 0.234
6.653 ± 0.309
1.616 ± 0.158
6.547 ± 0.234
7.257 ± 0.339
8.639 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.333 ± 0.149
5.376 ± 0.21
3.738 ± 0.255
4.365 ± 0.232
4.478 ± 0.219
6.607 ± 0.303
6.162 ± 0.283
6.486 ± 0.216
1.382 ± 0.136
3.353 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here