Gordonia phage Boopy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K7W8|A0A5C1K7W8_9CAUD Uncharacterized protein OS=Gordonia phage Boopy OX=2590875 GN=116 PE=4 SV=1
MM1 pKa = 7.48AVTAKK6 pKa = 10.7LYY8 pKa = 10.98GPFLQSVFNKK18 pKa = 10.18EE19 pKa = 3.26VDD21 pKa = 3.87LDD23 pKa = 3.92NDD25 pKa = 3.98TLKK28 pKa = 11.15VMLCTSSYY36 pKa = 10.65TPNQDD41 pKa = 2.35THH43 pKa = 7.02RR44 pKa = 11.84YY45 pKa = 7.99KK46 pKa = 10.94SSVTNEE52 pKa = 3.99VVGTGYY58 pKa = 8.21TATGATLTGVTVAYY72 pKa = 10.39DD73 pKa = 3.23SATNTFTLDD82 pKa = 3.28ASDD85 pKa = 4.07VTWAASTITARR96 pKa = 11.84YY97 pKa = 8.78AVFYY101 pKa = 10.0DD102 pKa = 4.1ASPASDD108 pKa = 3.04ATRR111 pKa = 11.84PLICYY116 pKa = 9.04WDD118 pKa = 4.41FGQDD122 pKa = 3.36EE123 pKa = 4.71VSSSGNFTLTLSASGLVAATVAA145 pKa = 3.79

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K718|A0A5C1K718_9CAUD Uncharacterized protein OS=Gordonia phage Boopy OX=2590875 GN=193 PE=4 SV=1
MM1 pKa = 7.82SYY3 pKa = 10.02TIPQRR8 pKa = 11.84ITCVLGLCIGGMIAISSNVCVLRR31 pKa = 11.84TVPDD35 pKa = 3.25IQIPISSHH43 pKa = 4.22SGKK46 pKa = 10.28RR47 pKa = 11.84LIKK50 pKa = 10.0NGRR53 pKa = 11.84RR54 pKa = 11.84STAAVVAADD63 pKa = 3.55QNLWNARR70 pKa = 11.84HH71 pKa = 4.54VHH73 pKa = 6.0RR74 pKa = 11.84LILSFIRR81 pKa = 11.84LYY83 pKa = 8.8STKK86 pKa = 10.76AKK88 pKa = 10.31FIFVMIHH95 pKa = 4.6FTMNQEE101 pKa = 4.12LTDD104 pKa = 3.72GG105 pKa = 3.99

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

162

0

162

34002

33

1998

209.9

23.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.067 ± 0.281

0.932 ± 0.112

6.467 ± 0.16

6.179 ± 0.227

3.206 ± 0.111

6.923 ± 0.28

2.076 ± 0.113

5.679 ± 0.167

5.406 ± 0.217

7.85 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.097

4.129 ± 0.123

5.059 ± 0.178

3.341 ± 0.097

6.167 ± 0.248

6.347 ± 0.197

6.603 ± 0.219

6.894 ± 0.124

1.982 ± 0.08

3.188 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski