Clostridiaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; unclassified Clostridiaceae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L9H7C2|A0A6L9H7C2_9CLOT HlyC/CorC family transporter OS=Clostridiaceae bacterium OX=1898204 GN=D3Z50_03740 PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 10.56KK3 pKa = 8.92MLSVAMAGMLALSLTACGSSEE24 pKa = 4.63KK25 pKa = 10.06PAEE28 pKa = 4.29TQAPAPTEE36 pKa = 3.98AVKK39 pKa = 10.21TEE41 pKa = 3.93AAVEE45 pKa = 4.11EE46 pKa = 4.61AVTEE50 pKa = 4.1AVKK53 pKa = 10.7EE54 pKa = 4.09DD55 pKa = 3.49AASEE59 pKa = 4.06GGYY62 pKa = 9.13TIGFSPYY69 pKa = 9.64TLTNEE74 pKa = 4.01YY75 pKa = 8.52FTAVQTGVQAACDD88 pKa = 3.66EE89 pKa = 5.11LGCEE93 pKa = 4.75MISFDD98 pKa = 4.7PQNDD102 pKa = 3.54PTKK105 pKa = 10.25QASQIEE111 pKa = 4.3DD112 pKa = 3.74MIASGIDD119 pKa = 3.09ALVYY123 pKa = 10.22IPYY126 pKa = 10.1DD127 pKa = 3.11SAGAHH132 pKa = 5.32TVLQTCRR139 pKa = 11.84DD140 pKa = 3.55AGIKK144 pKa = 9.44VINVDD149 pKa = 3.77NVITEE154 pKa = 4.2DD155 pKa = 4.61DD156 pKa = 4.07YY157 pKa = 12.08EE158 pKa = 4.38LVDD161 pKa = 6.25GIIASDD167 pKa = 3.71NTQLGYY173 pKa = 11.19LSGQWVAANHH183 pKa = 6.83PDD185 pKa = 3.36GANILIVHH193 pKa = 6.7LQTAEE198 pKa = 3.84SCIINVEE205 pKa = 4.44GFWSGIKK212 pKa = 10.59DD213 pKa = 3.59NVSNADD219 pKa = 3.24AFVEE223 pKa = 4.38VQVVEE228 pKa = 4.45GEE230 pKa = 4.45GATDD234 pKa = 3.1AAFNVVSDD242 pKa = 4.45ALQAHH247 pKa = 7.32DD248 pKa = 6.0DD249 pKa = 3.71IDD251 pKa = 4.35VIYY254 pKa = 10.25CINDD258 pKa = 3.44TSALGAVQAVEE269 pKa = 4.24EE270 pKa = 4.25AGKK273 pKa = 10.58AGSIDD278 pKa = 3.47ILGKK282 pKa = 10.48DD283 pKa = 3.44GAPIGKK289 pKa = 8.83HH290 pKa = 5.47AIKK293 pKa = 10.4DD294 pKa = 3.58GTMVQSSAQRR304 pKa = 11.84PTYY307 pKa = 8.39MGYY310 pKa = 9.03MGVQNAVKK318 pKa = 9.91ALKK321 pKa = 10.81GEE323 pKa = 3.98EE324 pKa = 4.21FEE326 pKa = 4.51FNIAIEE332 pKa = 4.53SYY334 pKa = 10.93SIDD337 pKa = 3.37ASNIDD342 pKa = 5.67DD343 pKa = 4.37YY344 pKa = 12.14DD345 pKa = 5.04LDD347 pKa = 3.68AWDD350 pKa = 5.08ALDD353 pKa = 3.56

Molecular weight: 37.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L9HCR9|A0A6L9HCR9_9CLOT ATP-binding protein OS=Clostridiaceae bacterium OX=1898204 GN=D3Z50_05840 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4379

0

4379

1312029

35

1611

299.6

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.643 ± 0.042

1.581 ± 0.016

5.384 ± 0.03

7.688 ± 0.043

4.119 ± 0.027

7.872 ± 0.04

1.687 ± 0.016

6.523 ± 0.033

5.816 ± 0.035

9.16 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.017

3.664 ± 0.027

3.572 ± 0.022

3.174 ± 0.021

5.912 ± 0.04

5.797 ± 0.031

4.875 ± 0.026

6.438 ± 0.029

1.033 ± 0.011

3.985 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski