Neurospora tetrasperma (strain FGSC 2509 / P0656)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Neurospora; Neurospora tetrasperma

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4UPZ8|G4UPZ8_NEUT9 Uncharacterized protein OS=Neurospora tetrasperma (strain FGSC 2509 / P0656) OX=510952 GN=NEUTE2DRAFT_139498 PE=4 SV=1
MM1 pKa = 7.57ANEE4 pKa = 3.97QSMPLPLVDD13 pKa = 3.3EE14 pKa = 5.21RR15 pKa = 11.84NRR17 pKa = 11.84KK18 pKa = 8.31MLDD21 pKa = 3.75AIHH24 pKa = 6.45NAPCMSLQIVLFSLYY39 pKa = 10.99AEE41 pKa = 4.92GDD43 pKa = 3.9DD44 pKa = 3.89NLKK47 pKa = 9.06TRR49 pKa = 11.84IEE51 pKa = 4.42DD52 pKa = 3.6LVHH55 pKa = 7.49DD56 pKa = 4.38YY57 pKa = 10.86TLTLHH62 pKa = 6.6NLDD65 pKa = 4.68AGIATPASEE74 pKa = 5.19LRR76 pKa = 11.84TEE78 pKa = 4.46PATEE82 pKa = 4.01VPLATEE88 pKa = 4.21EE89 pKa = 4.32PVDD92 pKa = 3.56NTIYY96 pKa = 11.0CPVCDD101 pKa = 5.89DD102 pKa = 4.44YY103 pKa = 11.86FDD105 pKa = 6.11PDD107 pKa = 5.63DD108 pKa = 5.0EE109 pKa = 7.23DD110 pKa = 4.95DD111 pKa = 4.5LCFHH115 pKa = 7.06HH116 pKa = 7.08PGKK119 pKa = 10.64RR120 pKa = 11.84IAIPGFDD127 pKa = 2.84SVAYY131 pKa = 8.62EE132 pKa = 4.26KK133 pKa = 10.65EE134 pKa = 4.28VSPEE138 pKa = 3.44AYY140 pKa = 9.54QIKK143 pKa = 10.04VEE145 pKa = 4.22EE146 pKa = 4.49GLDD149 pKa = 3.54TEE151 pKa = 4.54EE152 pKa = 4.5NRR154 pKa = 11.84LVPLWASTWDD164 pKa = 3.41CCGGVGASRR173 pKa = 11.84GCVQGVHH180 pKa = 4.84VEE182 pKa = 4.19YY183 pKa = 11.21VEE185 pKa = 5.9GMEE188 pKa = 5.25DD189 pKa = 3.39WDD191 pKa = 5.22DD192 pKa = 4.04SDD194 pKa = 5.26SSSSDD199 pKa = 3.31EE200 pKa = 4.34SDD202 pKa = 3.02SDD204 pKa = 3.6

Molecular weight: 22.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4V1S4|G4V1S4_NEUT9 AMPK1_CBM domain-containing protein OS=Neurospora tetrasperma (strain FGSC 2509 / P0656) OX=510952 GN=NEUTE2DRAFT_160384 PE=4 SV=1
VV1 pKa = 6.8RR2 pKa = 11.84AILRR6 pKa = 11.84VLKK9 pKa = 10.49RR10 pKa = 11.84RR11 pKa = 11.84NFVGKK16 pKa = 9.94NGNFNIRR23 pKa = 11.84CHH25 pKa = 5.21QLNRR29 pKa = 11.84PSFLVGVRR37 pKa = 11.84TWRR40 pKa = 11.84FLVRR44 pKa = 11.84AGRR47 pKa = 11.84LTLSKK52 pKa = 10.23KK53 pKa = 10.66APLFRR58 pKa = 11.84PRR60 pKa = 11.84KK61 pKa = 7.15TVGG64 pKa = 2.81

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11177

0

11177

4951514

49

10513

443.0

48.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.601 ± 0.023

1.173 ± 0.01

5.604 ± 0.017

6.39 ± 0.029

3.426 ± 0.014

7.164 ± 0.027

2.475 ± 0.013

4.493 ± 0.016

5.075 ± 0.023

8.413 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.01

3.718 ± 0.015

6.501 ± 0.027

4.207 ± 0.025

6.211 ± 0.022

8.281 ± 0.03

6.124 ± 0.018

6.002 ± 0.015

1.386 ± 0.009

2.601 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski