Amino acid dipepetide frequency for Novosphingobium sp. MBES04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.763AlaAla: 16.763 ± 0.158
1.213AlaCys: 1.213 ± 0.027
6.909AlaAsp: 6.909 ± 0.072
7.557AlaGlu: 7.557 ± 0.082
4.329AlaPhe: 4.329 ± 0.056
10.794AlaGly: 10.794 ± 0.092
2.582AlaHis: 2.582 ± 0.056
6.286AlaIle: 6.286 ± 0.072
3.913AlaLys: 3.913 ± 0.062
14.519AlaLeu: 14.519 ± 0.128
3.976AlaMet: 3.976 ± 0.053
3.123AlaAsn: 3.123 ± 0.048
6.891AlaPro: 6.891 ± 0.086
5.291AlaGln: 5.291 ± 0.068
10.588AlaArg: 10.588 ± 0.107
7.135AlaSer: 7.135 ± 0.077
6.001AlaThr: 6.001 ± 0.063
7.804AlaVal: 7.804 ± 0.086
1.77AlaTrp: 1.77 ± 0.037
2.602AlaTyr: 2.602 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.149CysAla: 1.149 ± 0.031
0.111CysCys: 0.111 ± 0.009
0.564CysAsp: 0.564 ± 0.02
0.539CysGlu: 0.539 ± 0.019
0.267CysPhe: 0.267 ± 0.014
0.935CysGly: 0.935 ± 0.026
0.229CysHis: 0.229 ± 0.014
0.339CysIle: 0.339 ± 0.015
0.165CysLys: 0.165 ± 0.012
0.772CysLeu: 0.772 ± 0.022
0.157CysMet: 0.157 ± 0.01
0.225CysAsn: 0.225 ± 0.013
0.496CysPro: 0.496 ± 0.019
0.223CysGln: 0.223 ± 0.012
0.578CysArg: 0.578 ± 0.02
0.503CysSer: 0.503 ± 0.02
0.444CysThr: 0.444 ± 0.017
0.585CysVal: 0.585 ± 0.019
0.126CysTrp: 0.126 ± 0.009
0.184CysTyr: 0.184 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.31AspAla: 7.31 ± 0.07
0.469AspCys: 0.469 ± 0.02
3.016AspAsp: 3.016 ± 0.051
3.61AspGlu: 3.61 ± 0.054
2.262AspPhe: 2.262 ± 0.036
5.463AspGly: 5.463 ± 0.067
1.243AspHis: 1.243 ± 0.033
2.855AspIle: 2.855 ± 0.047
1.669AspLys: 1.669 ± 0.041
6.027AspLeu: 6.027 ± 0.065
1.539AspMet: 1.539 ± 0.031
1.35AspAsn: 1.35 ± 0.035
3.736AspPro: 3.736 ± 0.049
1.501AspGln: 1.501 ± 0.035
3.814AspArg: 3.814 ± 0.058
2.23AspSer: 2.23 ± 0.041
3.111AspThr: 3.111 ± 0.048
4.015AspVal: 4.015 ± 0.052
1.163AspTrp: 1.163 ± 0.028
1.707AspTyr: 1.707 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
8.795GluAla: 8.795 ± 0.099
0.382GluCys: 0.382 ± 0.016
3.612GluAsp: 3.612 ± 0.058
3.841GluGlu: 3.841 ± 0.067
1.66GluPhe: 1.66 ± 0.034
5.484GluGly: 5.484 ± 0.068
1.196GluHis: 1.196 ± 0.029
3.045GluIle: 3.045 ± 0.049
2.128GluLys: 2.128 ± 0.049
5.486GluLeu: 5.486 ± 0.066
1.511GluMet: 1.511 ± 0.035
1.564GluAsn: 1.564 ± 0.034
2.724GluPro: 2.724 ± 0.05
1.995GluGln: 1.995 ± 0.034
5.073GluArg: 5.073 ± 0.073
2.42GluSer: 2.42 ± 0.044
3.593GluThr: 3.593 ± 0.053
4.14GluVal: 4.14 ± 0.055
0.823GluTrp: 0.823 ± 0.022
1.09GluTyr: 1.09 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.949PheAla: 4.949 ± 0.066
0.338PheCys: 0.338 ± 0.017
2.593PheAsp: 2.593 ± 0.052
2.184PheGlu: 2.184 ± 0.037
1.244PhePhe: 1.244 ± 0.03
3.648PheGly: 3.648 ± 0.052
0.718PheHis: 0.718 ± 0.021
1.309PheIle: 1.309 ± 0.031
0.847PheLys: 0.847 ± 0.024
3.22PheLeu: 3.22 ± 0.053
0.765PheMet: 0.765 ± 0.024
1.05PheAsn: 1.05 ± 0.029
1.531PhePro: 1.531 ± 0.034
0.949PheGln: 0.949 ± 0.028
1.964PheArg: 1.964 ± 0.035
1.929PheSer: 1.929 ± 0.037
2.203PheThr: 2.203 ± 0.044
2.65PheVal: 2.65 ± 0.047
0.547PheTrp: 0.547 ± 0.021
0.941PheTyr: 0.941 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.135GlyAla: 10.135 ± 0.095
0.832GlyCys: 0.832 ± 0.028
4.617GlyAsp: 4.617 ± 0.063
5.689GlyGlu: 5.689 ± 0.068
3.56GlyPhe: 3.56 ± 0.051
7.795GlyGly: 7.795 ± 0.104
1.965GlyHis: 1.965 ± 0.04
4.364GlyIle: 4.364 ± 0.06
3.243GlyLys: 3.243 ± 0.057
8.916GlyLeu: 8.916 ± 0.078
2.401GlyMet: 2.401 ± 0.039
2.321GlyAsn: 2.321 ± 0.054
3.841GlyPro: 3.841 ± 0.057
3.063GlyGln: 3.063 ± 0.056
6.054GlyArg: 6.054 ± 0.076
4.934GlySer: 4.934 ± 0.071
5.188GlyThr: 5.188 ± 0.074
5.987GlyVal: 5.987 ± 0.07
1.547GlyTrp: 1.547 ± 0.038
2.43GlyTyr: 2.43 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.558HisAla: 2.558 ± 0.042
0.22HisCys: 0.22 ± 0.014
1.239HisAsp: 1.239 ± 0.028
1.252HisGlu: 1.252 ± 0.03
0.859HisPhe: 0.859 ± 0.024
1.989HisGly: 1.989 ± 0.04
0.549HisHis: 0.549 ± 0.022
0.882HisIle: 0.882 ± 0.022
0.472HisLys: 0.472 ± 0.018
1.924HisLeu: 1.924 ± 0.042
0.463HisMet: 0.463 ± 0.018
0.435HisAsn: 0.435 ± 0.019
1.292HisPro: 1.292 ± 0.029
0.526HisGln: 0.526 ± 0.02
1.343HisArg: 1.343 ± 0.038
0.965HisSer: 0.965 ± 0.031
0.889HisThr: 0.889 ± 0.028
1.525HisVal: 1.525 ± 0.033
0.374HisTrp: 0.374 ± 0.017
0.593HisTyr: 0.593 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.325IleAla: 7.325 ± 0.078
0.459IleCys: 0.459 ± 0.019
3.398IleAsp: 3.398 ± 0.051
3.528IleGlu: 3.528 ± 0.052
1.399IlePhe: 1.399 ± 0.033
4.503IleGly: 4.503 ± 0.052
0.846IleHis: 0.846 ± 0.027
1.682IleIle: 1.682 ± 0.04
1.167IleLys: 1.167 ± 0.03
3.767IleLeu: 3.767 ± 0.067
0.894IleMet: 0.894 ± 0.026
1.272IleAsn: 1.272 ± 0.03
2.151IlePro: 2.151 ± 0.038
1.114IleGln: 1.114 ± 0.029
2.808IleArg: 2.808 ± 0.044
2.572IleSer: 2.572 ± 0.04
2.528IleThr: 2.528 ± 0.046
3.694IleVal: 3.694 ± 0.056
0.591IleTrp: 0.591 ± 0.022
1.038IleTyr: 1.038 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
4.155LysAla: 4.155 ± 0.07
0.144LysCys: 0.144 ± 0.008
1.675LysAsp: 1.675 ± 0.038
1.372LysGlu: 1.372 ± 0.034
0.752LysPhe: 0.752 ± 0.026
2.882LysGly: 2.882 ± 0.043
0.512LysHis: 0.512 ± 0.016
1.29LysIle: 1.29 ± 0.033
0.954LysLys: 0.954 ± 0.029
2.891LysLeu: 2.891 ± 0.057
0.684LysMet: 0.684 ± 0.022
0.672LysAsn: 0.672 ± 0.02
1.747LysPro: 1.747 ± 0.038
0.788LysGln: 0.788 ± 0.02
1.966LysArg: 1.966 ± 0.043
1.498LysSer: 1.498 ± 0.036
1.627LysThr: 1.627 ± 0.037
2.385LysVal: 2.385 ± 0.044
0.343LysTrp: 0.343 ± 0.014
0.577LysTyr: 0.577 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
14.718LeuAla: 14.718 ± 0.128
0.873LeuCys: 0.873 ± 0.028
6.444LeuAsp: 6.444 ± 0.068
5.81LeuGlu: 5.81 ± 0.077
3.542LeuPhe: 3.542 ± 0.053
8.714LeuGly: 8.714 ± 0.083
1.911LeuHis: 1.911 ± 0.038
4.195LeuIle: 4.195 ± 0.06
2.841LeuLys: 2.841 ± 0.049
9.018LeuLeu: 9.018 ± 0.12
2.085LeuMet: 2.085 ± 0.037
2.265LeuAsn: 2.265 ± 0.042
5.519LeuPro: 5.519 ± 0.071
2.644LeuGln: 2.644 ± 0.045
6.922LeuArg: 6.922 ± 0.08
6.001LeuSer: 6.001 ± 0.066
5.504LeuThr: 5.504 ± 0.068
7.513LeuVal: 7.513 ± 0.083
1.193LeuTrp: 1.193 ± 0.029
1.939LeuTyr: 1.939 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
3.457MetAla: 3.457 ± 0.052
0.164MetCys: 0.164 ± 0.01
1.21MetAsp: 1.21 ± 0.029
1.208MetGlu: 1.208 ± 0.034
0.688MetPhe: 0.688 ± 0.023
2.126MetGly: 2.126 ± 0.043
0.453MetHis: 0.453 ± 0.018
1.22MetIle: 1.22 ± 0.026
0.839MetLys: 0.839 ± 0.025
2.497MetLeu: 2.497 ± 0.042
0.655MetMet: 0.655 ± 0.022
0.724MetAsn: 0.724 ± 0.021
1.511MetPro: 1.511 ± 0.036
0.786MetGln: 0.786 ± 0.025
1.772MetArg: 1.772 ± 0.034
1.496MetSer: 1.496 ± 0.035
1.762MetThr: 1.762 ± 0.037
1.74MetVal: 1.74 ± 0.033
0.218MetTrp: 0.218 ± 0.011
0.308MetTyr: 0.308 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.241AsnAla: 3.241 ± 0.052
0.232AsnCys: 0.232 ± 0.013
1.344AsnAsp: 1.344 ± 0.031
1.223AsnGlu: 1.223 ± 0.031
1.024AsnPhe: 1.024 ± 0.029
2.326AsnGly: 2.326 ± 0.05
0.47AsnHis: 0.47 ± 0.017
1.132AsnIle: 1.132 ± 0.027
0.552AsnLys: 0.552 ± 0.02
2.482AsnLeu: 2.482 ± 0.046
0.574AsnMet: 0.574 ± 0.022
0.6AsnAsn: 0.6 ± 0.025
1.75AsnPro: 1.75 ± 0.034
0.719AsnGln: 0.719 ± 0.025
1.752AsnArg: 1.752 ± 0.034
1.252AsnSer: 1.252 ± 0.03
1.327AsnThr: 1.327 ± 0.035
1.864AsnVal: 1.864 ± 0.038
0.472AsnTrp: 0.472 ± 0.019
0.747AsnTyr: 0.747 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
6.779ProAla: 6.779 ± 0.086
0.398ProCys: 0.398 ± 0.017
3.707ProAsp: 3.707 ± 0.058
4.366ProGlu: 4.366 ± 0.067
1.899ProPhe: 1.899 ± 0.034
4.936ProGly: 4.936 ± 0.058
1.05ProHis: 1.05 ± 0.028
2.21ProIle: 2.21 ± 0.043
1.326ProLys: 1.326 ± 0.038
4.897ProLeu: 4.897 ± 0.07
1.315ProMet: 1.315 ± 0.031
1.133ProAsn: 1.133 ± 0.029
2.377ProPro: 2.377 ± 0.054
1.756ProGln: 1.756 ± 0.033
3.321ProArg: 3.321 ± 0.051
2.894ProSer: 2.894 ± 0.042
2.591ProThr: 2.591 ± 0.037
4.224ProVal: 4.224 ± 0.061
0.678ProTrp: 0.678 ± 0.023
1.129ProTyr: 1.129 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.202GlnAla: 4.202 ± 0.056
0.262GlnCys: 0.262 ± 0.014
1.697GlnAsp: 1.697 ± 0.037
1.612GlnGlu: 1.612 ± 0.037
1.079GlnPhe: 1.079 ± 0.024
2.798GlnGly: 2.798 ± 0.048
0.577GlnHis: 0.577 ± 0.021
1.784GlnIle: 1.784 ± 0.033
0.843GlnLys: 0.843 ± 0.024
2.964GlnLeu: 2.964 ± 0.049
0.906GlnMet: 0.906 ± 0.026
0.786GlnAsn: 0.786 ± 0.024
1.643GlnPro: 1.643 ± 0.037
1.073GlnGln: 1.073 ± 0.035
2.464GlnArg: 2.464 ± 0.047
1.737GlnSer: 1.737 ± 0.037
1.698GlnThr: 1.698 ± 0.035
2.47GlnVal: 2.47 ± 0.041
0.432GlnTrp: 0.432 ± 0.018
0.647GlnTyr: 0.647 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.71ArgAla: 8.71 ± 0.095
0.52ArgCys: 0.52 ± 0.019
3.944ArgAsp: 3.944 ± 0.051
4.71ArgGlu: 4.71 ± 0.064
3.034ArgPhe: 3.034 ± 0.045
4.927ArgGly: 4.927 ± 0.07
1.697ArgHis: 1.697 ± 0.037
3.949ArgIle: 3.949 ± 0.048
2.234ArgLys: 2.234 ± 0.04
7.75ArgLeu: 7.75 ± 0.095
1.841ArgMet: 1.841 ± 0.034
1.728ArgAsn: 1.728 ± 0.037
3.521ArgPro: 3.521 ± 0.049
2.383ArgGln: 2.383 ± 0.047
5.613ArgArg: 5.613 ± 0.083
3.742ArgSer: 3.742 ± 0.06
3.752ArgThr: 3.752 ± 0.05
4.649ArgVal: 4.649 ± 0.06
1.131ArgTrp: 1.131 ± 0.03
1.912ArgTyr: 1.912 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.788SerAla: 6.788 ± 0.069
0.466SerCys: 0.466 ± 0.016
3.154SerAsp: 3.154 ± 0.052
2.983SerGlu: 2.983 ± 0.053
2.092SerPhe: 2.092 ± 0.045
5.495SerGly: 5.495 ± 0.071
1.0SerHis: 1.0 ± 0.028
2.282SerIle: 2.282 ± 0.04
1.374SerLys: 1.374 ± 0.035
5.432SerLeu: 5.432 ± 0.064
1.279SerMet: 1.279 ± 0.032
1.359SerAsn: 1.359 ± 0.031
2.969SerPro: 2.969 ± 0.047
1.745SerGln: 1.745 ± 0.04
3.648SerArg: 3.648 ± 0.052
3.124SerSer: 3.124 ± 0.059
2.767SerThr: 2.767 ± 0.042
3.734SerVal: 3.734 ± 0.053
0.858SerTrp: 0.858 ± 0.024
1.428SerTyr: 1.428 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.107ThrAla: 6.107 ± 0.07
0.514ThrCys: 0.514 ± 0.019
2.762ThrAsp: 2.762 ± 0.046
2.542ThrGlu: 2.542 ± 0.043
2.038ThrPhe: 2.038 ± 0.035
5.455ThrGly: 5.455 ± 0.072
1.027ThrHis: 1.027 ± 0.026
2.785ThrIle: 2.785 ± 0.044
1.307ThrLys: 1.307 ± 0.03
6.078ThrLeu: 6.078 ± 0.066
1.308ThrMet: 1.308 ± 0.032
1.386ThrAsn: 1.386 ± 0.032
3.513ThrPro: 3.513 ± 0.053
1.699ThrGln: 1.699 ± 0.035
3.886ThrArg: 3.886 ± 0.05
3.106ThrSer: 3.106 ± 0.052
2.889ThrThr: 2.889 ± 0.055
3.91ThrVal: 3.91 ± 0.054
0.772ThrTrp: 0.772 ± 0.027
1.353ThrTyr: 1.353 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
8.735ValAla: 8.735 ± 0.074
0.604ValCys: 0.604 ± 0.022
3.926ValAsp: 3.926 ± 0.059
4.543ValGlu: 4.543 ± 0.057
2.367ValPhe: 2.367 ± 0.039
5.357ValGly: 5.357 ± 0.07
1.426ValHis: 1.426 ± 0.037
3.527ValIle: 3.527 ± 0.053
1.93ValLys: 1.93 ± 0.044
7.131ValLeu: 7.131 ± 0.078
1.607ValMet: 1.607 ± 0.036
1.95ValAsn: 1.95 ± 0.041
4.019ValPro: 4.019 ± 0.052
2.019ValGln: 2.019 ± 0.04
5.09ValArg: 5.09 ± 0.069
4.233ValSer: 4.233 ± 0.051
4.47ValThr: 4.47 ± 0.062
5.079ValVal: 5.079 ± 0.064
0.9ValTrp: 0.9 ± 0.024
1.403ValTyr: 1.403 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.371TrpAla: 1.371 ± 0.033
0.165TrpCys: 0.165 ± 0.011
0.754TrpAsp: 0.754 ± 0.022
0.634TrpGlu: 0.634 ± 0.02
0.592TrpPhe: 0.592 ± 0.021
1.031TrpGly: 1.031 ± 0.027
0.402TrpHis: 0.402 ± 0.017
0.73TrpIle: 0.73 ± 0.024
0.504TrpLys: 0.504 ± 0.018
1.689TrpLeu: 1.689 ± 0.039
0.396TrpMet: 0.396 ± 0.018
0.496TrpAsn: 0.496 ± 0.017
0.7TrpPro: 0.7 ± 0.025
0.673TrpGln: 0.673 ± 0.022
1.26TrpArg: 1.26 ± 0.034
0.969TrpSer: 0.969 ± 0.029
0.805TrpThr: 0.805 ± 0.028
0.81TrpVal: 0.81 ± 0.024
0.231TrpTrp: 0.231 ± 0.012
0.317TrpTyr: 0.317 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.8TyrAla: 2.8 ± 0.044
0.225TyrCys: 0.225 ± 0.013
1.528TyrAsp: 1.528 ± 0.032
1.36TyrGlu: 1.36 ± 0.031
0.889TyrPhe: 0.889 ± 0.024
2.225TyrGly: 2.225 ± 0.041
0.499TyrHis: 0.499 ± 0.018
0.872TyrIle: 0.872 ± 0.024
0.605TyrLys: 0.605 ± 0.021
2.193TyrLeu: 2.193 ± 0.043
0.474TyrMet: 0.474 ± 0.018
0.643TyrAsn: 0.643 ± 0.02
1.054TyrPro: 1.054 ± 0.028
0.726TyrGln: 0.726 ± 0.023
1.831TyrArg: 1.831 ± 0.041
1.238TyrSer: 1.238 ± 0.029
1.288TyrThr: 1.288 ± 0.031
1.538TyrVal: 1.538 ± 0.033
0.358TyrTrp: 0.358 ± 0.019
0.669TyrTyr: 0.669 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4731 proteins (1456332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski