Cyclobacterium lianum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Cyclobacterium

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7NUB9|A0A1M7NUB9_9BACT Glucose/arabinose dehydrogenase beta-propeller fold OS=Cyclobacterium lianum OX=388280 GN=SAMN04488057_10678 PE=4 SV=1
MM1 pKa = 6.68MTYY4 pKa = 10.3KK5 pKa = 10.49HH6 pKa = 5.89MRR8 pKa = 11.84IEE10 pKa = 4.09NVTYY14 pKa = 10.43RR15 pKa = 11.84GVLTLLFGSLCIISCEE31 pKa = 4.13TADD34 pKa = 4.13TGTGMDD40 pKa = 4.41DD41 pKa = 3.48PGLPGSTGSTFACTEE56 pKa = 3.97FSYY59 pKa = 11.53SDD61 pKa = 3.06TLYY64 pKa = 10.87FINEE68 pKa = 3.86QTEE71 pKa = 4.05QLINPQTEE79 pKa = 3.97LAGTYY84 pKa = 9.59SASPEE89 pKa = 4.06GLDD92 pKa = 3.99IDD94 pKa = 4.55PLSGAIDD101 pKa = 3.42INASEE106 pKa = 4.63TGLKK110 pKa = 9.49YY111 pKa = 10.49QISFSPDD118 pKa = 2.64NGAPSCPVFLTIGGINYY135 pKa = 9.47LDD137 pKa = 3.3QVYY140 pKa = 10.54VLDD143 pKa = 3.9QGEE146 pKa = 4.35TQAIPVYY153 pKa = 10.65NGVPSAAIPCEE164 pKa = 4.68DD165 pKa = 5.62DD166 pKa = 6.3DD167 pKa = 7.7DD168 pKa = 7.6DD169 pKa = 7.69DD170 pKa = 7.66DD171 pKa = 7.68DD172 pKa = 7.65DD173 pKa = 7.65DD174 pKa = 7.65DD175 pKa = 7.65DD176 pKa = 7.65DD177 pKa = 7.65DD178 pKa = 7.65DD179 pKa = 7.61DD180 pKa = 7.59DD181 pKa = 7.58DD182 pKa = 7.79DD183 pKa = 5.57DD184 pKa = 5.25DD185 pKa = 5.55CRR187 pKa = 11.84FDD189 pKa = 4.99ADD191 pKa = 4.97GPDD194 pKa = 3.75GQNLADD200 pKa = 4.42AGVAINASTGTIDD213 pKa = 6.33LEE215 pKa = 4.44TTLNNGALGSSPANGTFSDD234 pKa = 4.68FSLYY238 pKa = 10.45YY239 pKa = 10.4RR240 pKa = 11.84LNDD243 pKa = 3.74PSMGALNRR251 pKa = 11.84IGLRR255 pKa = 11.84VYY257 pKa = 10.85YY258 pKa = 10.44FEE260 pKa = 4.28TTADD264 pKa = 3.74VPQSLLDD271 pKa = 4.38EE272 pKa = 4.92IEE274 pKa = 4.22TKK276 pKa = 10.6RR277 pKa = 11.84NQILGTQATGQHH289 pKa = 6.0LRR291 pKa = 11.84TSNDD295 pKa = 2.98NPRR298 pKa = 11.84VRR300 pKa = 11.84PRR302 pKa = 11.84PPYY305 pKa = 9.87LVIVSRR311 pKa = 11.84LRR313 pKa = 3.41

Molecular weight: 33.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7PV89|A0A1M7PV89_9BACT RND family efflux transporter MFP subunit OS=Cyclobacterium lianum OX=388280 GN=SAMN04488057_1114 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.18RR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.53ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84HH40 pKa = 5.24KK41 pKa = 10.87LSVSSEE47 pKa = 3.97KK48 pKa = 9.91TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4679

0

4679

1677476

10

5137

358.5

40.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.116 ± 0.03

0.7 ± 0.011

5.572 ± 0.027

6.805 ± 0.03

5.084 ± 0.028

7.391 ± 0.032

1.975 ± 0.018

6.735 ± 0.029

5.897 ± 0.043

9.985 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.017

4.94 ± 0.036

4.217 ± 0.023

3.798 ± 0.02

4.723 ± 0.023

6.437 ± 0.024

4.907 ± 0.023

6.127 ± 0.026

1.35 ± 0.014

3.787 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski