Hubei orthoptera virus 4
Average proteome isoelectric point is 7.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KH43|A0A1L3KH43_9VIRU Capsid protein OS=Hubei orthoptera virus 4 OX=1923012 PE=4 SV=1
MM1 pKa = 7.65 SPTTPKK7 pKa = 10.09 MSAKK11 pKa = 9.09 TLRR14 pKa = 11.84 NRR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 9.61 RR19 pKa = 11.84 LARR22 pKa = 11.84 KK23 pKa = 8.98 ARR25 pKa = 11.84 QTAANSQLLPGIGYY39 pKa = 7.33 NTNRR43 pKa = 11.84 NGVAPSRR50 pKa = 11.84 ADD52 pKa = 3.06 PRR54 pKa = 11.84 TFNLLNNLGNTIDD67 pKa = 4.49 GRR69 pKa = 11.84 AACMKK74 pKa = 9.84 ILHH77 pKa = 6.85 PCMEE81 pKa = 4.66 TGNHH85 pKa = 4.5 VTKK88 pKa = 10.65 FPDD91 pKa = 3.86 GAVSTSVVLEE101 pKa = 3.86 RR102 pKa = 11.84 RR103 pKa = 11.84 DD104 pKa = 3.78 EE105 pKa = 4.18 YY106 pKa = 11.27 SIPPPKK112 pKa = 10.67 DD113 pKa = 3.34 FGAVKK118 pKa = 10.72 LEE120 pKa = 4.01 TWSCLVFHH128 pKa = 6.96 LPFAMCRR135 pKa = 11.84 TVVCRR140 pKa = 11.84 YY141 pKa = 8.97 HH142 pKa = 6.79 AALPVSEE149 pKa = 4.37 QRR151 pKa = 11.84 VEE153 pKa = 3.87 TALAAMVALFLDD165 pKa = 3.41 QDD167 pKa = 3.82 HH168 pKa = 6.52 NKK170 pKa = 10.52 EE171 pKa = 4.02 YY172 pKa = 10.07 IYY174 pKa = 10.36 PNWTDD179 pKa = 3.37 KK180 pKa = 11.38 DD181 pKa = 3.28 IGPTDD186 pKa = 3.8 NGNISQWSVLTSDD199 pKa = 3.49 TLTPEE204 pKa = 4.37 LLLNSGSDD212 pKa = 3.4 VMRR215 pKa = 11.84 SIRR218 pKa = 11.84 RR219 pKa = 11.84 TCMGVTVDD227 pKa = 5.13 LDD229 pKa = 3.88 APALANQGRR238 pKa = 11.84 VITGQWGPDD247 pKa = 3.23 VALGTLSFNKK257 pKa = 10.22 DD258 pKa = 2.88 NAVEE262 pKa = 3.97 VRR264 pKa = 11.84 DD265 pKa = 4.19 IYY267 pKa = 11.04 DD268 pKa = 3.41 INDD271 pKa = 4.05 PAWTTNAIVSSDD283 pKa = 3.61 EE284 pKa = 4.11 FCRR287 pKa = 11.84 QAQAKK292 pKa = 8.32 YY293 pKa = 10.89 GSYY296 pKa = 10.17 TPIRR300 pKa = 11.84 PIGLDD305 pKa = 3.41 VPFTPITEE313 pKa = 3.96 KK314 pKa = 10.63 RR315 pKa = 11.84 VISMRR320 pKa = 11.84 SNLAGINFDD329 pKa = 3.57 TVEE332 pKa = 4.1 AFGDD336 pKa = 3.25 LWLRR340 pKa = 11.84 GWCTAVEE347 pKa = 4.3 SWDD350 pKa = 3.45 GMDD353 pKa = 3.64 RR354 pKa = 11.84 SASLRR359 pKa = 11.84 MKK361 pKa = 9.99 IRR363 pKa = 11.84 EE364 pKa = 4.59 DD365 pKa = 3.33 IEE367 pKa = 5.56 LIAAPKK373 pKa = 10.17 SPYY376 pKa = 10.55 SPFSTPALPDD386 pKa = 3.62 DD387 pKa = 3.73 LRR389 pKa = 11.84 ARR391 pKa = 11.84 TMIQEE396 pKa = 4.44 FSRR399 pKa = 11.84 TQPHH403 pKa = 7.0 SYY405 pKa = 9.37 PAEE408 pKa = 4.27 DD409 pKa = 3.65 NEE411 pKa = 4.59 FGKK414 pKa = 10.67 LVPKK418 pKa = 10.12 ILSGIAGALSNLGIPVVSPIASVVGNVADD447 pKa = 3.77 SAFGKK452 pKa = 9.53 MVGDD456 pKa = 3.66 WLDD459 pKa = 3.24 KK460 pKa = 11.25 LFF462 pKa = 4.86
Molecular weight: 50.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.425
IPC2_protein 6.453
IPC_protein 6.576
Toseland 6.517
ProMoST 6.927
Dawson 7.073
Bjellqvist 7.0
Wikipedia 7.029
Rodwell 7.059
Grimsley 6.59
Solomon 7.102
Lehninger 7.102
Nozaki 7.337
DTASelect 7.351
Thurlkill 7.38
EMBOSS 7.41
Sillero 7.497
Patrickios 4.469
IPC_peptide 7.102
IPC2_peptide 7.0
IPC2.peptide.svr19 6.887
Protein with the highest isoelectric point:
>tr|A0A1L3KH43|A0A1L3KH43_9VIRU Capsid protein OS=Hubei orthoptera virus 4 OX=1923012 PE=4 SV=1
MM1 pKa = 7.56 TKK3 pKa = 9.71 QSNYY7 pKa = 8.54 IQHH10 pKa = 7.19 DD11 pKa = 3.76 VLPRR15 pKa = 11.84 DD16 pKa = 4.09 GQDD19 pKa = 3.23 PSPWAMVAKK28 pKa = 10.56 HH29 pKa = 5.22 MFLRR33 pKa = 11.84 VYY35 pKa = 10.21 HH36 pKa = 6.6 IIWFIVYY43 pKa = 7.7 LVCNCGFPTVRR54 pKa = 11.84 EE55 pKa = 3.79 TSEE58 pKa = 3.62 WVEE61 pKa = 4.03 AVITRR66 pKa = 11.84 PKK68 pKa = 10.32 NLRR71 pKa = 11.84 HH72 pKa = 6.05 LFLNTEE78 pKa = 3.95 VMSIFPCRR86 pKa = 11.84 TSHH89 pKa = 6.03 SHH91 pKa = 6.02 PTSAMYY97 pKa = 8.37 RR98 pKa = 11.84 TAVNNFMNTLATRR111 pKa = 11.84 AGMEE115 pKa = 4.11 PFNVSKK121 pKa = 11.08 SPTDD125 pKa = 3.26 GGKK128 pKa = 8.12 GTRR131 pKa = 11.84 YY132 pKa = 9.81 FYY134 pKa = 10.74 GAKK137 pKa = 10.25 DD138 pKa = 3.29 LATPYY143 pKa = 10.83 VDD145 pKa = 5.65 DD146 pKa = 4.25 KK147 pKa = 11.75 VGNNDD152 pKa = 3.75 VIIMCDD158 pKa = 2.72 VDD160 pKa = 4.3 YY161 pKa = 10.19 YY162 pKa = 11.43 TDD164 pKa = 3.38 MTKK167 pKa = 9.7 WLSYY171 pKa = 9.75 FRR173 pKa = 11.84 PILMYY178 pKa = 10.21 TFVPTSAAARR188 pKa = 11.84 RR189 pKa = 11.84 KK190 pKa = 10.0 DD191 pKa = 3.42 YY192 pKa = 10.74 AYY194 pKa = 11.04 RR195 pKa = 11.84 LIDD198 pKa = 3.52 DD199 pKa = 3.72 HH200 pKa = 9.58 VEE202 pKa = 3.74 FHH204 pKa = 6.31 VAGGSSYY211 pKa = 10.37 RR212 pKa = 11.84 HH213 pKa = 6.11 KK214 pKa = 10.58 LWNYY218 pKa = 8.61 KK219 pKa = 9.77 GDD221 pKa = 3.89 YY222 pKa = 10.33 VSTVDD227 pKa = 4.96 DD228 pKa = 4.14 SGNLLVFHH236 pKa = 6.87 LEE238 pKa = 3.63 QRR240 pKa = 11.84 NIIGDD245 pKa = 3.63 PDD247 pKa = 3.11 HH248 pKa = 7.25 RR249 pKa = 11.84 YY250 pKa = 9.45 IVLTPLAKK258 pKa = 10.01 IPSPFHH264 pKa = 5.53 WFLPKK269 pKa = 10.16 FEE271 pKa = 5.44 PIRR274 pKa = 11.84 RR275 pKa = 11.84 HH276 pKa = 6.42 SFLQDD281 pKa = 3.22 GTSVLYY287 pKa = 10.42 EE288 pKa = 4.63 PINDD292 pKa = 4.2 TLSLAPNGAWHH303 pKa = 6.49 SVDD306 pKa = 3.81 ILGTQYY312 pKa = 11.47 SAIKK316 pKa = 10.09 QRR318 pKa = 11.84 LLNKK322 pKa = 9.77 SAPPVMSDD330 pKa = 3.0 IEE332 pKa = 4.17 RR333 pKa = 11.84 LLRR336 pKa = 11.84 ASGDD340 pKa = 3.56 EE341 pKa = 4.04 NAVTNAPLIFNLMHH355 pKa = 7.07 LNFHH359 pKa = 6.56 KK360 pKa = 10.96 NVVLTNGVVSSFQPLGSLATEE381 pKa = 4.65 DD382 pKa = 4.29 GKK384 pKa = 10.15 PSGKK388 pKa = 9.59 QLTPPLVSEE397 pKa = 4.27 PAVFPTKK404 pKa = 10.4 GVNADD409 pKa = 3.14 EE410 pKa = 4.18 ATIKK414 pKa = 10.87 GRR416 pKa = 11.84 ITSVANPVVPPRR428 pKa = 11.84 KK429 pKa = 9.74 YY430 pKa = 10.37 KK431 pKa = 10.68 DD432 pKa = 3.45 YY433 pKa = 11.54 ANEE436 pKa = 3.85 FTNCIVKK443 pKa = 10.38 RR444 pKa = 11.84 AGTGSPLSVDD454 pKa = 4.07 EE455 pKa = 4.43 VRR457 pKa = 11.84 KK458 pKa = 9.08 LQPNPQQKK466 pKa = 10.28 ARR468 pKa = 11.84 FNKK471 pKa = 9.51 VEE473 pKa = 4.2 ATLTTTPQNDD483 pKa = 3.09 LKK485 pKa = 11.46 AFIKK489 pKa = 10.61 SEE491 pKa = 4.02 AYY493 pKa = 10.59 GNVTDD498 pKa = 4.75 PRR500 pKa = 11.84 NITTMSPEE508 pKa = 4.15 LTVMLSGFTYY518 pKa = 10.65 AFKK521 pKa = 10.6 QDD523 pKa = 3.51 CLKK526 pKa = 9.62 NQKK529 pKa = 9.01 WYY531 pKa = 10.92 GPGKK535 pKa = 9.02 TPTQTLKK542 pKa = 11.23 ALAALSDD549 pKa = 5.35 LSDD552 pKa = 2.95 EE553 pKa = 4.37 WLTVDD558 pKa = 4.42 YY559 pKa = 11.15 SRR561 pKa = 11.84 LDD563 pKa = 3.44 GTVSEE568 pKa = 4.42 FLQRR572 pKa = 11.84 SVVMATYY579 pKa = 9.62 LKK581 pKa = 10.03 WVAEE585 pKa = 4.02 EE586 pKa = 4.43 HH587 pKa = 6.27 KK588 pKa = 11.17 AEE590 pKa = 4.49 LKK592 pKa = 10.86 HH593 pKa = 5.66 EE594 pKa = 4.3 LEE596 pKa = 5.14 AIFIKK601 pKa = 10.41 SGRR604 pKa = 11.84 TANGVKK610 pKa = 10.21 FNPGYY615 pKa = 8.45 GTRR618 pKa = 11.84 SGSPITTDD626 pKa = 3.32 GNTMICAYY634 pKa = 10.14 VVYY637 pKa = 10.56 CCFRR641 pKa = 11.84 EE642 pKa = 4.08 LGYY645 pKa = 10.49 KK646 pKa = 9.85 PKK648 pKa = 10.51 EE649 pKa = 4.04 AFDD652 pKa = 4.62 SIGLVYY658 pKa = 10.62 GDD660 pKa = 5.23 DD661 pKa = 4.01 GAFPALPGLKK671 pKa = 9.55 EE672 pKa = 3.8 KK673 pKa = 11.39 LEE675 pKa = 4.18 IVSKK679 pKa = 10.86 QLGLKK684 pKa = 9.94 IKK686 pKa = 10.72 LDD688 pKa = 3.52 VSKK691 pKa = 11.06 VGEE694 pKa = 4.04 PLPYY698 pKa = 10.23 LGRR701 pKa = 11.84 FFVDD705 pKa = 4.19 PKK707 pKa = 8.81 TCKK710 pKa = 10.32 DD711 pKa = 3.41 SFQDD715 pKa = 4.07 PIRR718 pKa = 11.84 TISKK722 pKa = 9.83 LHH724 pKa = 5.18 LTANKK729 pKa = 9.04 TVTPEE734 pKa = 3.27 QALANKK740 pKa = 9.45 AHH742 pKa = 7.1 GYY744 pKa = 9.69 LATDD748 pKa = 3.64 SKK750 pKa = 10.34 TPIIGTWAAKK760 pKa = 10.19 VIEE763 pKa = 4.06 LTGRR767 pKa = 11.84 KK768 pKa = 7.39 TKK770 pKa = 10.69 GLLRR774 pKa = 11.84 EE775 pKa = 4.18 EE776 pKa = 4.17 AFKK779 pKa = 11.1 LSNAWPQANKK789 pKa = 10.79 SLITEE794 pKa = 4.56 AMAKK798 pKa = 10.18 VLDD801 pKa = 4.15 LQLSEE806 pKa = 6.35 LIALDD811 pKa = 3.44 QKK813 pKa = 10.9 INQVTALDD821 pKa = 3.8 QLPVLLEE828 pKa = 4.14 TVRR831 pKa = 11.84 EE832 pKa = 4.18 TKK834 pKa = 10.28 IPAVLGDD841 pKa = 3.76 EE842 pKa = 4.34 VVEE845 pKa = 4.49 PGLHH849 pKa = 5.31 VKK851 pKa = 10.42 SIEE854 pKa = 4.19 SKK856 pKa = 8.98 QVKK859 pKa = 8.37 DD860 pKa = 3.99 TKK862 pKa = 10.69 PKK864 pKa = 10.89 DD865 pKa = 3.29 VDD867 pKa = 3.5 APTTGAADD875 pKa = 3.75 SVPSLDD881 pKa = 4.26 KK882 pKa = 10.94 DD883 pKa = 4.26 SKK885 pKa = 11.0 CSCQTSSITTAGKK898 pKa = 10.23 ASNVRR903 pKa = 11.84 GNVRR907 pKa = 11.84 QNGDD911 pKa = 2.87 GAGRR915 pKa = 11.84 GGRR918 pKa = 11.84 GHH920 pKa = 6.62 GPSARR925 pKa = 11.84 FRR927 pKa = 11.84 SRR929 pKa = 11.84 RR930 pKa = 11.84 GNQHH934 pKa = 5.02 SAKK937 pKa = 9.75 PDD939 pKa = 3.5 LRR941 pKa = 11.84 SGNSNGSPLKK951 pKa = 10.44 SDD953 pKa = 3.45 LNTRR957 pKa = 11.84 EE958 pKa = 3.81 KK959 pKa = 9.77 TAEE962 pKa = 3.95 RR963 pKa = 11.84 VV964 pKa = 3.25
Molecular weight: 106.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.366
IPC2_protein 8.331
IPC_protein 8.17
Toseland 8.77
ProMoST 8.741
Dawson 9.165
Bjellqvist 9.048
Wikipedia 9.297
Rodwell 9.355
Grimsley 9.136
Solomon 9.194
Lehninger 9.165
Nozaki 9.18
DTASelect 8.946
Thurlkill 9.063
EMBOSS 9.311
Sillero 9.238
Patrickios 4.647
IPC_peptide 9.194
IPC2_peptide 7.688
IPC2.peptide.svr19 7.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1426
462
964
713.0
78.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.784 ± 0.601
1.403 ± 0.181
6.241 ± 0.378
4.348 ± 0.249
3.647 ± 0.101
6.452 ± 0.096
2.034 ± 0.405
4.769 ± 0.354
6.311 ± 1.212
8.766 ± 0.179
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.104 ± 0.391
5.049 ± 0.2
6.522 ± 0.223
2.805 ± 0.234
5.54 ± 0.645
7.153 ± 0.114
7.363 ± 0.241
7.223 ± 0.163
1.473 ± 0.262
3.015 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here