Amino acid dipepetide frequency for Psychrobacter phage pOW20-A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.183AlaAla: 8.183 ± 1.568
0.708AlaCys: 0.708 ± 0.353
5.507AlaAsp: 5.507 ± 0.509
4.406AlaGlu: 4.406 ± 0.612
2.203AlaPhe: 2.203 ± 0.435
5.35AlaGly: 5.35 ± 0.813
1.652AlaHis: 1.652 ± 0.392
6.609AlaIle: 6.609 ± 0.855
5.665AlaLys: 5.665 ± 0.781
5.665AlaLeu: 5.665 ± 0.669
3.619AlaMet: 3.619 ± 0.565
4.485AlaAsn: 4.485 ± 0.52
1.81AlaPro: 1.81 ± 0.41
4.642AlaGln: 4.642 ± 0.682
3.068AlaArg: 3.068 ± 0.525
4.485AlaSer: 4.485 ± 0.438
4.406AlaThr: 4.406 ± 0.728
5.744AlaVal: 5.744 ± 0.672
0.708AlaTrp: 0.708 ± 0.222
2.439AlaTyr: 2.439 ± 0.41
0.0AlaXaa: 0.0 ± 0.0
Cys
0.315CysAla: 0.315 ± 0.148
0.157CysCys: 0.157 ± 0.107
0.472CysAsp: 0.472 ± 0.188
0.551CysGlu: 0.551 ± 0.27
0.393CysPhe: 0.393 ± 0.17
1.259CysGly: 1.259 ± 0.307
0.551CysHis: 0.551 ± 0.237
0.551CysIle: 0.551 ± 0.218
0.708CysLys: 0.708 ± 0.253
1.101CysLeu: 1.101 ± 0.308
0.0CysMet: 0.0 ± 0.0
0.393CysAsn: 0.393 ± 0.172
0.157CysPro: 0.157 ± 0.1
0.551CysGln: 0.551 ± 0.264
0.708CysArg: 0.708 ± 0.207
1.023CysSer: 1.023 ± 0.258
0.629CysThr: 0.629 ± 0.186
0.472CysVal: 0.472 ± 0.268
0.157CysTrp: 0.157 ± 0.113
0.315CysTyr: 0.315 ± 0.231
0.0CysXaa: 0.0 ± 0.0
Asp
6.845AspAla: 6.845 ± 0.884
0.472AspCys: 0.472 ± 0.203
4.878AspAsp: 4.878 ± 0.707
5.901AspGlu: 5.901 ± 0.702
2.911AspPhe: 2.911 ± 0.397
5.665AspGly: 5.665 ± 0.823
0.944AspHis: 0.944 ± 0.327
4.485AspIle: 4.485 ± 0.523
4.091AspLys: 4.091 ± 0.596
5.35AspLeu: 5.35 ± 0.731
2.518AspMet: 2.518 ± 0.639
4.406AspAsn: 4.406 ± 0.521
1.259AspPro: 1.259 ± 0.266
1.888AspGln: 1.888 ± 0.361
2.046AspArg: 2.046 ± 0.332
4.485AspSer: 4.485 ± 0.623
4.249AspThr: 4.249 ± 0.58
4.249AspVal: 4.249 ± 0.508
1.731AspTrp: 1.731 ± 0.341
3.068AspTyr: 3.068 ± 0.496
0.0AspXaa: 0.0 ± 0.0
Glu
3.777GluAla: 3.777 ± 0.538
0.865GluCys: 0.865 ± 0.282
2.675GluAsp: 2.675 ± 0.449
1.495GluGlu: 1.495 ± 0.434
2.518GluPhe: 2.518 ± 0.469
3.619GluGly: 3.619 ± 0.495
1.416GluHis: 1.416 ± 0.324
4.799GluIle: 4.799 ± 0.6
3.304GluLys: 3.304 ± 0.589
7.002GluLeu: 7.002 ± 0.732
1.731GluMet: 1.731 ± 0.4
2.439GluAsn: 2.439 ± 0.504
2.124GluPro: 2.124 ± 0.386
3.226GluGln: 3.226 ± 0.582
3.383GluArg: 3.383 ± 0.478
3.462GluSer: 3.462 ± 0.544
3.462GluThr: 3.462 ± 0.48
4.799GluVal: 4.799 ± 0.709
1.495GluTrp: 1.495 ± 0.285
2.832GluTyr: 2.832 ± 0.471
0.0GluXaa: 0.0 ± 0.0
Phe
2.832PheAla: 2.832 ± 0.438
0.315PheCys: 0.315 ± 0.17
3.304PheAsp: 3.304 ± 0.469
2.124PheGlu: 2.124 ± 0.36
1.101PhePhe: 1.101 ± 0.298
3.147PheGly: 3.147 ± 0.517
0.393PheHis: 0.393 ± 0.196
2.36PheIle: 2.36 ± 0.433
2.282PheLys: 2.282 ± 0.395
1.731PheLeu: 1.731 ± 0.474
1.259PheMet: 1.259 ± 0.301
2.596PheAsn: 2.596 ± 0.583
1.18PhePro: 1.18 ± 0.314
0.708PheGln: 0.708 ± 0.281
1.731PheArg: 1.731 ± 0.358
2.439PheSer: 2.439 ± 0.418
2.282PheThr: 2.282 ± 0.361
2.596PheVal: 2.596 ± 0.426
0.393PheTrp: 0.393 ± 0.187
0.787PheTyr: 0.787 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
4.485GlyAla: 4.485 ± 0.717
0.865GlyCys: 0.865 ± 0.296
6.53GlyAsp: 6.53 ± 0.873
4.17GlyGlu: 4.17 ± 0.667
3.855GlyPhe: 3.855 ± 0.569
5.665GlyGly: 5.665 ± 0.851
1.416GlyHis: 1.416 ± 0.298
4.17GlyIle: 4.17 ± 0.664
5.114GlyLys: 5.114 ± 0.776
6.845GlyLeu: 6.845 ± 0.764
2.282GlyMet: 2.282 ± 0.36
3.304GlyAsn: 3.304 ± 0.482
0.236GlyPro: 0.236 ± 0.13
3.541GlyGln: 3.541 ± 0.487
3.147GlyArg: 3.147 ± 0.539
3.462GlySer: 3.462 ± 0.527
3.855GlyThr: 3.855 ± 0.611
5.114GlyVal: 5.114 ± 0.656
1.101GlyTrp: 1.101 ± 0.329
3.698GlyTyr: 3.698 ± 0.566
0.0GlyXaa: 0.0 ± 0.0
His
0.944HisAla: 0.944 ± 0.23
0.079HisCys: 0.079 ± 0.074
1.652HisAsp: 1.652 ± 0.366
0.944HisGlu: 0.944 ± 0.302
0.629HisPhe: 0.629 ± 0.242
1.416HisGly: 1.416 ± 0.311
0.472HisHis: 0.472 ± 0.216
1.023HisIle: 1.023 ± 0.286
0.787HisLys: 0.787 ± 0.257
0.787HisLeu: 0.787 ± 0.288
0.393HisMet: 0.393 ± 0.18
0.629HisAsn: 0.629 ± 0.225
0.629HisPro: 0.629 ± 0.213
0.944HisGln: 0.944 ± 0.28
0.787HisArg: 0.787 ± 0.282
1.574HisSer: 1.574 ± 0.374
0.865HisThr: 0.865 ± 0.229
1.023HisVal: 1.023 ± 0.257
0.236HisTrp: 0.236 ± 0.135
1.101HisTyr: 1.101 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
7.002IleAla: 7.002 ± 0.901
0.472IleCys: 0.472 ± 0.212
6.766IleAsp: 6.766 ± 0.653
6.452IleGlu: 6.452 ± 0.662
1.574IlePhe: 1.574 ± 0.405
5.271IleGly: 5.271 ± 0.738
0.944IleHis: 0.944 ± 0.288
4.485IleIle: 4.485 ± 0.533
5.901IleLys: 5.901 ± 0.592
3.619IleLeu: 3.619 ± 0.502
1.81IleMet: 1.81 ± 0.363
3.619IleAsn: 3.619 ± 0.647
1.731IlePro: 1.731 ± 0.379
2.518IleGln: 2.518 ± 0.372
2.911IleArg: 2.911 ± 0.434
3.462IleSer: 3.462 ± 0.517
2.99IleThr: 2.99 ± 0.481
2.99IleVal: 2.99 ± 0.441
0.865IleTrp: 0.865 ± 0.268
2.124IleTyr: 2.124 ± 0.412
0.0IleXaa: 0.0 ± 0.0
Lys
5.665LysAla: 5.665 ± 0.839
0.708LysCys: 0.708 ± 0.291
4.327LysAsp: 4.327 ± 0.63
3.698LysGlu: 3.698 ± 0.607
1.967LysPhe: 1.967 ± 0.42
4.249LysGly: 4.249 ± 0.435
0.708LysHis: 0.708 ± 0.216
3.934LysIle: 3.934 ± 0.492
4.013LysLys: 4.013 ± 0.614
5.035LysLeu: 5.035 ± 0.548
1.495LysMet: 1.495 ± 0.311
3.068LysAsn: 3.068 ± 0.574
2.675LysPro: 2.675 ± 0.425
3.304LysGln: 3.304 ± 0.467
3.383LysArg: 3.383 ± 0.459
4.563LysSer: 4.563 ± 0.616
4.878LysThr: 4.878 ± 0.735
3.855LysVal: 3.855 ± 0.612
1.18LysTrp: 1.18 ± 0.281
1.495LysTyr: 1.495 ± 0.333
0.0LysXaa: 0.0 ± 0.0
Leu
6.845LeuAla: 6.845 ± 0.726
1.101LeuCys: 1.101 ± 0.266
5.507LeuAsp: 5.507 ± 0.555
4.327LeuGlu: 4.327 ± 0.721
2.596LeuPhe: 2.596 ± 0.471
5.98LeuGly: 5.98 ± 0.767
1.259LeuHis: 1.259 ± 0.312
5.586LeuIle: 5.586 ± 0.53
4.878LeuLys: 4.878 ± 0.696
7.632LeuLeu: 7.632 ± 1.052
2.203LeuMet: 2.203 ± 0.376
4.327LeuAsn: 4.327 ± 0.565
3.147LeuPro: 3.147 ± 0.432
3.619LeuGln: 3.619 ± 0.422
2.911LeuArg: 2.911 ± 0.515
5.507LeuSer: 5.507 ± 0.606
5.429LeuThr: 5.429 ± 0.637
5.271LeuVal: 5.271 ± 0.566
1.023LeuTrp: 1.023 ± 0.262
1.495LeuTyr: 1.495 ± 0.305
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.482
0.629MetCys: 0.629 ± 0.198
1.81MetAsp: 1.81 ± 0.391
1.18MetGlu: 1.18 ± 0.26
1.416MetPhe: 1.416 ± 0.292
1.81MetGly: 1.81 ± 0.42
0.315MetHis: 0.315 ± 0.165
1.574MetIle: 1.574 ± 0.348
1.023MetLys: 1.023 ± 0.285
2.596MetLeu: 2.596 ± 0.47
0.865MetMet: 0.865 ± 0.272
1.101MetAsn: 1.101 ± 0.275
1.101MetPro: 1.101 ± 0.355
1.652MetGln: 1.652 ± 0.388
1.18MetArg: 1.18 ± 0.318
2.911MetSer: 2.911 ± 0.459
3.383MetThr: 3.383 ± 0.455
1.652MetVal: 1.652 ± 0.38
0.079MetTrp: 0.079 ± 0.077
0.551MetTyr: 0.551 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
4.406AsnAla: 4.406 ± 0.564
0.551AsnCys: 0.551 ± 0.21
2.911AsnAsp: 2.911 ± 0.513
3.698AsnGlu: 3.698 ± 0.554
1.652AsnPhe: 1.652 ± 0.343
4.249AsnGly: 4.249 ± 0.671
0.787AsnHis: 0.787 ± 0.277
2.911AsnIle: 2.911 ± 0.583
3.619AsnLys: 3.619 ± 0.594
2.911AsnLeu: 2.911 ± 0.407
1.652AsnMet: 1.652 ± 0.368
2.911AsnAsn: 2.911 ± 0.518
2.518AsnPro: 2.518 ± 0.519
2.439AsnGln: 2.439 ± 0.574
2.124AsnArg: 2.124 ± 0.438
2.754AsnSer: 2.754 ± 0.403
2.99AsnThr: 2.99 ± 0.494
3.147AsnVal: 3.147 ± 0.538
0.708AsnTrp: 0.708 ± 0.258
2.046AsnTyr: 2.046 ± 0.328
0.0AsnXaa: 0.0 ± 0.0
Pro
1.731ProAla: 1.731 ± 0.312
0.236ProCys: 0.236 ± 0.129
2.203ProAsp: 2.203 ± 0.486
2.203ProGlu: 2.203 ± 0.37
1.023ProPhe: 1.023 ± 0.266
0.551ProGly: 0.551 ± 0.181
0.865ProHis: 0.865 ± 0.232
2.124ProIle: 2.124 ± 0.365
2.046ProLys: 2.046 ± 0.378
2.675ProLeu: 2.675 ± 0.455
1.18ProMet: 1.18 ± 0.34
1.731ProAsn: 1.731 ± 0.411
1.416ProPro: 1.416 ± 0.326
1.259ProGln: 1.259 ± 0.287
1.023ProArg: 1.023 ± 0.25
2.439ProSer: 2.439 ± 0.48
2.675ProThr: 2.675 ± 0.405
2.36ProVal: 2.36 ± 0.384
0.315ProTrp: 0.315 ± 0.16
1.338ProTyr: 1.338 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
4.563GlnAla: 4.563 ± 0.722
0.393GlnCys: 0.393 ± 0.183
2.518GlnAsp: 2.518 ± 0.459
1.338GlnGlu: 1.338 ± 0.283
1.574GlnPhe: 1.574 ± 0.378
3.226GlnGly: 3.226 ± 0.57
0.708GlnHis: 0.708 ± 0.285
2.203GlnIle: 2.203 ± 0.329
2.124GlnLys: 2.124 ± 0.417
4.406GlnLeu: 4.406 ± 0.663
1.731GlnMet: 1.731 ± 0.42
2.124GlnAsn: 2.124 ± 0.377
2.046GlnPro: 2.046 ± 0.451
3.068GlnGln: 3.068 ± 0.719
2.832GlnArg: 2.832 ± 0.454
3.462GlnSer: 3.462 ± 0.481
2.282GlnThr: 2.282 ± 0.393
2.518GlnVal: 2.518 ± 0.406
0.708GlnTrp: 0.708 ± 0.244
1.495GlnTyr: 1.495 ± 0.338
0.0GlnXaa: 0.0 ± 0.0
Arg
3.068ArgAla: 3.068 ± 0.467
0.708ArgCys: 0.708 ± 0.268
3.462ArgAsp: 3.462 ± 0.616
2.518ArgGlu: 2.518 ± 0.498
1.81ArgPhe: 1.81 ± 0.409
2.832ArgGly: 2.832 ± 0.397
1.101ArgHis: 1.101 ± 0.301
2.754ArgIle: 2.754 ± 0.434
2.203ArgLys: 2.203 ± 0.469
4.563ArgLeu: 4.563 ± 0.538
1.101ArgMet: 1.101 ± 0.299
1.81ArgAsn: 1.81 ± 0.359
1.967ArgPro: 1.967 ± 0.364
2.124ArgGln: 2.124 ± 0.344
2.832ArgArg: 2.832 ± 0.477
2.596ArgSer: 2.596 ± 0.385
2.282ArgThr: 2.282 ± 0.375
2.124ArgVal: 2.124 ± 0.459
0.393ArgTrp: 0.393 ± 0.16
1.18ArgTyr: 1.18 ± 0.302
0.0ArgXaa: 0.0 ± 0.0
Ser
4.249SerAla: 4.249 ± 0.725
0.865SerCys: 0.865 ± 0.249
4.799SerAsp: 4.799 ± 0.527
4.406SerGlu: 4.406 ± 0.473
2.282SerPhe: 2.282 ± 0.362
5.665SerGly: 5.665 ± 0.723
0.393SerHis: 0.393 ± 0.159
5.271SerIle: 5.271 ± 0.675
4.799SerLys: 4.799 ± 0.597
5.507SerLeu: 5.507 ± 0.637
1.81SerMet: 1.81 ± 0.438
3.777SerAsn: 3.777 ± 0.604
1.81SerPro: 1.81 ± 0.296
2.282SerGln: 2.282 ± 0.429
2.36SerArg: 2.36 ± 0.463
5.429SerSer: 5.429 ± 0.628
3.698SerThr: 3.698 ± 0.486
3.855SerVal: 3.855 ± 0.596
0.708SerTrp: 0.708 ± 0.22
1.967SerTyr: 1.967 ± 0.374
0.0SerXaa: 0.0 ± 0.0
Thr
5.665ThrAla: 5.665 ± 0.722
0.472ThrCys: 0.472 ± 0.227
5.114ThrAsp: 5.114 ± 0.678
3.855ThrGlu: 3.855 ± 0.583
1.652ThrPhe: 1.652 ± 0.323
4.721ThrGly: 4.721 ± 0.618
1.101ThrHis: 1.101 ± 0.28
4.013ThrIle: 4.013 ± 0.56
4.013ThrLys: 4.013 ± 0.648
4.799ThrLeu: 4.799 ± 0.545
1.101ThrMet: 1.101 ± 0.278
2.36ThrAsn: 2.36 ± 0.427
2.282ThrPro: 2.282 ± 0.423
2.518ThrGln: 2.518 ± 0.416
1.967ThrArg: 1.967 ± 0.402
4.799ThrSer: 4.799 ± 0.673
3.147ThrThr: 3.147 ± 0.506
4.642ThrVal: 4.642 ± 0.54
0.787ThrTrp: 0.787 ± 0.222
1.731ThrTyr: 1.731 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
4.327ValAla: 4.327 ± 0.573
0.315ValCys: 0.315 ± 0.145
3.777ValAsp: 3.777 ± 0.635
4.485ValGlu: 4.485 ± 0.597
2.518ValPhe: 2.518 ± 0.441
4.799ValGly: 4.799 ± 0.634
0.944ValHis: 0.944 ± 0.274
5.271ValIle: 5.271 ± 0.802
5.271ValLys: 5.271 ± 0.659
3.934ValLeu: 3.934 ± 0.528
1.574ValMet: 1.574 ± 0.289
3.383ValAsn: 3.383 ± 0.509
2.282ValPro: 2.282 ± 0.413
2.124ValGln: 2.124 ± 0.354
3.147ValArg: 3.147 ± 0.594
3.934ValSer: 3.934 ± 0.563
4.327ValThr: 4.327 ± 0.526
4.013ValVal: 4.013 ± 0.539
1.18ValTrp: 1.18 ± 0.344
2.124ValTyr: 2.124 ± 0.413
0.0ValXaa: 0.0 ± 0.0
Trp
1.18TrpAla: 1.18 ± 0.264
0.236TrpCys: 0.236 ± 0.147
1.18TrpAsp: 1.18 ± 0.352
0.551TrpGlu: 0.551 ± 0.186
0.629TrpPhe: 0.629 ± 0.205
0.708TrpGly: 0.708 ± 0.224
0.472TrpHis: 0.472 ± 0.2
1.338TrpIle: 1.338 ± 0.368
0.629TrpLys: 0.629 ± 0.204
1.652TrpLeu: 1.652 ± 0.372
0.315TrpMet: 0.315 ± 0.153
0.393TrpAsn: 0.393 ± 0.165
0.157TrpPro: 0.157 ± 0.114
0.944TrpGln: 0.944 ± 0.259
0.315TrpArg: 0.315 ± 0.144
1.259TrpSer: 1.259 ± 0.311
0.551TrpThr: 0.551 ± 0.21
1.967TrpVal: 1.967 ± 0.45
0.157TrpTrp: 0.157 ± 0.102
0.315TrpTyr: 0.315 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.832TyrAla: 2.832 ± 0.595
0.315TyrCys: 0.315 ± 0.169
2.046TyrAsp: 2.046 ± 0.406
2.124TyrGlu: 2.124 ± 0.446
1.259TyrPhe: 1.259 ± 0.348
2.675TyrGly: 2.675 ± 0.473
0.472TyrHis: 0.472 ± 0.19
2.046TyrIle: 2.046 ± 0.413
1.731TyrLys: 1.731 ± 0.366
2.911TyrLeu: 2.911 ± 0.427
0.551TyrMet: 0.551 ± 0.188
2.124TyrAsn: 2.124 ± 0.397
0.865TyrPro: 0.865 ± 0.281
1.967TyrGln: 1.967 ± 0.331
1.574TyrArg: 1.574 ± 0.299
1.888TyrSer: 1.888 ± 0.391
2.36TyrThr: 2.36 ± 0.423
1.338TyrVal: 1.338 ± 0.342
1.023TyrTrp: 1.023 ± 0.293
0.865TyrTyr: 0.865 ± 0.321
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (12711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski