Arthrobacter phage Constance

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bridgettevirus; Arthrobacter virus Constance

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KEQ5|A0A3G2KEQ5_9CAUD Integrase OS=Arthrobacter phage Constance OX=2419950 GN=34 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.33LVVITTDD9 pKa = 3.3GGHH12 pKa = 6.25LTVEE16 pKa = 4.59DD17 pKa = 5.25FNASTLMDD25 pKa = 5.47LLDD28 pKa = 3.79SWSTQPYY35 pKa = 10.19LAFALDD41 pKa = 3.07NGMAYY46 pKa = 10.04FASHH50 pKa = 4.81QVTRR54 pKa = 11.84IDD56 pKa = 4.66AYY58 pKa = 11.01DD59 pKa = 3.22SS60 pKa = 3.54

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KER3|A0A3G2KER3_9CAUD Lysin A OS=Arthrobacter phage Constance OX=2419950 GN=25 PE=4 SV=1
MM1 pKa = 7.09GTPGRR6 pKa = 11.84NRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84PLHH13 pKa = 5.71TMRR16 pKa = 11.84QAHH19 pKa = 6.11PRR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 5.19IMGPRR28 pKa = 11.84PHH30 pKa = 7.09RR31 pKa = 11.84RR32 pKa = 11.84PHH34 pKa = 7.15HH35 pKa = 5.77MDD37 pKa = 3.17RR38 pKa = 11.84PRR40 pKa = 11.84TRR42 pKa = 11.84RR43 pKa = 11.84LQPQGRR49 pKa = 11.84RR50 pKa = 11.84QGHH53 pKa = 5.84PAPRR57 pKa = 11.84PPPLTAPEE65 pKa = 4.22GTPTPGGG72 pKa = 3.45

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13872

30

1298

198.2

21.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.399 ± 0.524

0.894 ± 0.134

6.012 ± 0.165

5.745 ± 0.265

2.588 ± 0.133

8.672 ± 0.331

1.86 ± 0.219

4.469 ± 0.158

4.678 ± 0.286

7.713 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.098 ± 0.134

3.078 ± 0.199

5.644 ± 0.42

4.21 ± 0.339

6.473 ± 0.413

5.457 ± 0.278

7.223 ± 0.395

6.596 ± 0.311

1.723 ± 0.193

2.465 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski