Amino acid dipepetide frequency for Actinomadura rubrisoli

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.817AlaAla: 21.817 ± 0.14	1.172AlaCys: 1.172 ± 0.023	8.292AlaAsp: 8.292 ± 0.058	8.689AlaGlu: 8.689 ± 0.062	3.685AlaPhe: 3.685 ± 0.036	13.828AlaGly: 13.828 ± 0.084	2.722AlaHis: 2.722 ± 0.036	4.1AlaIle: 4.1 ± 0.041	2.684AlaLys: 2.684 ± 0.037	14.548AlaLeu: 14.548 ± 0.099	2.617AlaMet: 2.617 ± 0.031	1.857AlaAsn: 1.857 ± 0.03	6.955AlaPro: 6.955 ± 0.062	3.388AlaGln: 3.388 ± 0.041	11.296AlaArg: 11.296 ± 0.069	5.835AlaSer: 5.835 ± 0.049	6.475AlaThr: 6.475 ± 0.049	11.705AlaVal: 11.705 ± 0.071	1.902AlaTrp: 1.902 ± 0.026	2.604AlaTyr: 2.604 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.02	0.107CysCys: 0.107 ± 0.007	0.55CysAsp: 0.55 ± 0.014	0.417CysGlu: 0.417 ± 0.012	0.226CysPhe: 0.226 ± 0.01	1.038CysGly: 1.038 ± 0.021	0.225CysHis: 0.225 ± 0.01	0.144CysIle: 0.144 ± 0.008	0.148CysLys: 0.148 ± 0.007	0.771CysLeu: 0.771 ± 0.016	0.135CysMet: 0.135 ± 0.008	0.137CysAsn: 0.137 ± 0.007	0.544CysPro: 0.544 ± 0.014	0.183CysGln: 0.183 ± 0.009	0.67CysArg: 0.67 ± 0.015	0.444CysSer: 0.444 ± 0.014	0.473CysThr: 0.473 ± 0.015	0.664CysVal: 0.664 ± 0.017	0.12CysTrp: 0.12 ± 0.007	0.18CysTyr: 0.18 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.825AspAla: 7.825 ± 0.058	0.402AspCys: 0.402 ± 0.013	3.913AspAsp: 3.913 ± 0.041	4.038AspGlu: 4.038 ± 0.045	1.525AspPhe: 1.525 ± 0.025	6.661AspGly: 6.661 ± 0.06	1.451AspHis: 1.451 ± 0.026	1.812AspIle: 1.812 ± 0.029	1.058AspLys: 1.058 ± 0.022	6.819AspLeu: 6.819 ± 0.054	0.825AspMet: 0.825 ± 0.017	0.871AspAsn: 0.871 ± 0.021	4.737AspPro: 4.737 ± 0.043	1.587AspGln: 1.587 ± 0.025	5.406AspArg: 5.406 ± 0.049	2.106AspSer: 2.106 ± 0.029	2.641AspThr: 2.641 ± 0.033	4.935AspVal: 4.935 ± 0.044	0.956AspTrp: 0.956 ± 0.017	1.145AspTyr: 1.145 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.059GluAla: 7.059 ± 0.064	0.395GluCys: 0.395 ± 0.013	2.744GluAsp: 2.744 ± 0.034	3.219GluGlu: 3.219 ± 0.039	1.498GluPhe: 1.498 ± 0.023	4.065GluGly: 4.065 ± 0.039	1.645GluHis: 1.645 ± 0.024	2.447GluIle: 2.447 ± 0.034	1.153GluLys: 1.153 ± 0.024	6.538GluLeu: 6.538 ± 0.058	0.999GluMet: 0.999 ± 0.019	0.918GluAsn: 0.918 ± 0.019	3.536GluPro: 3.536 ± 0.035	1.948GluGln: 1.948 ± 0.031	6.069GluArg: 6.069 ± 0.06	2.322GluSer: 2.322 ± 0.033	2.858GluThr: 2.858 ± 0.032	4.506GluVal: 4.506 ± 0.045	0.788GluTrp: 0.788 ± 0.017	1.039GluTyr: 1.039 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.716PheAla: 3.716 ± 0.045	0.273PheCys: 0.273 ± 0.01	2.011PheAsp: 2.011 ± 0.03	1.43PheGlu: 1.43 ± 0.023	0.846PhePhe: 0.846 ± 0.018	3.079PheGly: 3.079 ± 0.035	0.616PheHis: 0.616 ± 0.015	0.771PheIle: 0.771 ± 0.016	0.507PheLys: 0.507 ± 0.013	2.555PheLeu: 2.555 ± 0.03	0.428PheMet: 0.428 ± 0.012	0.556PheAsn: 0.556 ± 0.015	1.38PhePro: 1.38 ± 0.023	0.746PheGln: 0.746 ± 0.016	1.869PheArg: 1.869 ± 0.027	1.408PheSer: 1.408 ± 0.026	1.944PheThr: 1.944 ± 0.03	2.256PheVal: 2.256 ± 0.031	0.434PheTrp: 0.434 ± 0.013	0.584PheTyr: 0.584 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.245GlyAla: 11.245 ± 0.08	0.895GlyCys: 0.895 ± 0.019	5.658GlyAsp: 5.658 ± 0.05	5.235GlyGlu: 5.235 ± 0.052	2.905GlyPhe: 2.905 ± 0.037	9.482GlyGly: 9.482 ± 0.081	2.343GlyHis: 2.343 ± 0.034	3.382GlyIle: 3.382 ± 0.035	2.375GlyLys: 2.375 ± 0.031	9.803GlyLeu: 9.803 ± 0.067	2.028GlyMet: 2.028 ± 0.025	1.619GlyAsn: 1.619 ± 0.029	5.701GlyPro: 5.701 ± 0.049	2.564GlyGln: 2.564 ± 0.037	8.976GlyArg: 8.976 ± 0.068	4.868GlySer: 4.868 ± 0.046	6.021GlyThr: 6.021 ± 0.055	7.567GlyVal: 7.567 ± 0.056	1.752GlyTrp: 1.752 ± 0.025	2.185GlyTyr: 2.185 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.715HisAla: 2.715 ± 0.034	0.213HisCys: 0.213 ± 0.008	1.399HisAsp: 1.399 ± 0.019	1.172HisGlu: 1.172 ± 0.02	0.577HisPhe: 0.577 ± 0.016	2.359HisGly: 2.359 ± 0.031	0.682HisHis: 0.682 ± 0.018	0.696HisIle: 0.696 ± 0.017	0.319HisLys: 0.319 ± 0.01	2.448HisLeu: 2.448 ± 0.031	0.356HisMet: 0.356 ± 0.011	0.368HisAsn: 0.368 ± 0.012	1.737HisPro: 1.737 ± 0.029	0.623HisGln: 0.623 ± 0.016	2.235HisArg: 2.235 ± 0.035	0.942HisSer: 0.942 ± 0.019	1.107HisThr: 1.107 ± 0.02	1.775HisVal: 1.775 ± 0.024	0.352HisTrp: 0.352 ± 0.011	0.497HisTyr: 0.497 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.143IleAla: 5.143 ± 0.048	0.309IleCys: 0.309 ± 0.011	2.402IleAsp: 2.402 ± 0.032	2.204IleGlu: 2.204 ± 0.031	0.767IlePhe: 0.767 ± 0.017	3.77IleGly: 3.77 ± 0.036	0.596IleHis: 0.596 ± 0.015	1.018IleIle: 1.018 ± 0.019	0.715IleLys: 0.715 ± 0.014	2.608IleLeu: 2.608 ± 0.03	0.536IleMet: 0.536 ± 0.014	0.708IleAsn: 0.708 ± 0.017	1.864IlePro: 1.864 ± 0.03	0.747IleGln: 0.747 ± 0.017	2.51IleArg: 2.51 ± 0.03	1.738IleSer: 1.738 ± 0.025	2.33IleThr: 2.33 ± 0.03	3.213IleVal: 3.213 ± 0.037	0.396IleTrp: 0.396 ± 0.012	0.552IleTyr: 0.552 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.726LysAla: 2.726 ± 0.036	0.128LysCys: 0.128 ± 0.008	1.181LysAsp: 1.181 ± 0.022	1.037LysGlu: 1.037 ± 0.021	0.415LysPhe: 0.415 ± 0.013	1.769LysGly: 1.769 ± 0.03	0.409LysHis: 0.409 ± 0.012	0.927LysIle: 0.927 ± 0.019	0.659LysLys: 0.659 ± 0.02	1.754LysLeu: 1.754 ± 0.027	0.368LysMet: 0.368 ± 0.011	0.466LysAsn: 0.466 ± 0.015	1.329LysPro: 1.329 ± 0.025	0.577LysGln: 0.577 ± 0.015	1.534LysArg: 1.534 ± 0.024	1.073LysSer: 1.073 ± 0.023	1.221LysThr: 1.221 ± 0.022	1.815LysVal: 1.815 ± 0.029	0.279LysTrp: 0.279 ± 0.01	0.399LysTyr: 0.399 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	15.665LeuAla: 15.665 ± 0.088	0.845LeuCys: 0.845 ± 0.017	7.002LeuAsp: 7.002 ± 0.056	4.937LeuGlu: 4.937 ± 0.047	2.545LeuPhe: 2.545 ± 0.034	9.333LeuGly: 9.333 ± 0.066	2.227LeuHis: 2.227 ± 0.028	3.531LeuIle: 3.531 ± 0.038	1.875LeuLys: 1.875 ± 0.027	10.718LeuLeu: 10.718 ± 0.086	1.633LeuMet: 1.633 ± 0.023	1.752LeuAsn: 1.752 ± 0.026	6.485LeuPro: 6.485 ± 0.061	2.086LeuGln: 2.086 ± 0.028	9.297LeuArg: 9.297 ± 0.067	5.193LeuSer: 5.193 ± 0.049	6.271LeuThr: 6.271 ± 0.051	8.651LeuVal: 8.651 ± 0.071	1.33LeuTrp: 1.33 ± 0.024	1.786LeuTyr: 1.786 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.43MetAla: 2.43 ± 0.03	0.129MetCys: 0.129 ± 0.006	0.979MetAsp: 0.979 ± 0.021	0.78MetGlu: 0.78 ± 0.018	0.515MetPhe: 0.515 ± 0.014	1.339MetGly: 1.339 ± 0.023	0.345MetHis: 0.345 ± 0.013	0.794MetIle: 0.794 ± 0.017	0.392MetLys: 0.392 ± 0.012	1.816MetLeu: 1.816 ± 0.028	0.315MetMet: 0.315 ± 0.011	0.437MetAsn: 0.437 ± 0.011	1.168MetPro: 1.168 ± 0.02	0.401MetGln: 0.401 ± 0.013	1.656MetArg: 1.656 ± 0.022	1.307MetSer: 1.307 ± 0.021	1.491MetThr: 1.491 ± 0.027	1.377MetVal: 1.377 ± 0.021	0.229MetTrp: 0.229 ± 0.008	0.297MetTyr: 0.297 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.257AsnAla: 2.257 ± 0.034	0.169AsnCys: 0.169 ± 0.008	0.926AsnAsp: 0.926 ± 0.021	0.818AsnGlu: 0.818 ± 0.016	0.439AsnPhe: 0.439 ± 0.012	1.854AsnGly: 1.854 ± 0.032	0.358AsnHis: 0.358 ± 0.01	0.591AsnIle: 0.591 ± 0.015	0.347AsnLys: 0.347 ± 0.012	1.679AsnLeu: 1.679 ± 0.027	0.275AsnMet: 0.275 ± 0.01	0.352AsnAsn: 0.352 ± 0.013	1.304AsnPro: 1.304 ± 0.022	0.464AsnGln: 0.464 ± 0.015	1.318AsnArg: 1.318 ± 0.021	0.769AsnSer: 0.769 ± 0.019	0.994AsnThr: 0.994 ± 0.02	1.424AsnVal: 1.424 ± 0.024	0.251AsnTrp: 0.251 ± 0.01	0.373AsnTyr: 0.373 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.678ProAla: 8.678 ± 0.067	0.422ProCys: 0.422 ± 0.012	4.707ProAsp: 4.707 ± 0.045	4.104ProGlu: 4.104 ± 0.04	1.621ProPhe: 1.621 ± 0.024	7.481ProGly: 7.481 ± 0.068	1.325ProHis: 1.325 ± 0.024	1.706ProIle: 1.706 ± 0.026	1.223ProLys: 1.223 ± 0.02	5.221ProLeu: 5.221 ± 0.051	1.137ProMet: 1.137 ± 0.02	0.981ProAsn: 0.981 ± 0.021	4.37ProPro: 4.37 ± 0.054	1.556ProGln: 1.556 ± 0.025	4.458ProArg: 4.458 ± 0.046	3.614ProSer: 3.614 ± 0.039	2.808ProThr: 2.808 ± 0.031	5.234ProVal: 5.234 ± 0.048	0.921ProTrp: 0.921 ± 0.018	1.284ProTyr: 1.284 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.609GlnAla: 3.609 ± 0.037	0.175GlnCys: 0.175 ± 0.009	1.363GlnAsp: 1.363 ± 0.022	1.343GlnGlu: 1.343 ± 0.025	0.627GlnPhe: 0.627 ± 0.016	2.206GlnGly: 2.206 ± 0.031	0.555GlnHis: 0.555 ± 0.013	1.159GlnIle: 1.159 ± 0.022	0.516GlnLys: 0.516 ± 0.018	2.442GlnLeu: 2.442 ± 0.034	0.488GlnMet: 0.488 ± 0.013	0.504GlnAsn: 0.504 ± 0.015	1.503GlnPro: 1.503 ± 0.029	1.001GlnGln: 1.001 ± 0.03	2.491GlnArg: 2.491 ± 0.032	1.159GlnSer: 1.159 ± 0.02	1.27GlnThr: 1.27 ± 0.024	2.438GlnVal: 2.438 ± 0.028	0.441GlnTrp: 0.441 ± 0.013	0.496GlnTyr: 0.496 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	10.881ArgAla: 10.881 ± 0.076	0.68ArgCys: 0.68 ± 0.015	5.073ArgAsp: 5.073 ± 0.044	4.933ArgGlu: 4.933 ± 0.046	2.583ArgPhe: 2.583 ± 0.027	6.408ArgGly: 6.408 ± 0.053	2.301ArgHis: 2.301 ± 0.028	3.354ArgIle: 3.354 ± 0.041	1.682ArgLys: 1.682 ± 0.024	10.097ArgLeu: 10.097 ± 0.075	1.94ArgMet: 1.94 ± 0.026	1.396ArgAsn: 1.396 ± 0.021	5.947ArgPro: 5.947 ± 0.057	2.323ArgGln: 2.323 ± 0.029	9.1ArgArg: 9.1 ± 0.085	4.175ArgSer: 4.175 ± 0.042	5.106ArgThr: 5.106 ± 0.043	6.416ArgVal: 6.416 ± 0.05	1.527ArgTrp: 1.527 ± 0.027	1.884ArgTyr: 1.884 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.333SerAla: 6.333 ± 0.046	0.403SerCys: 0.403 ± 0.011	2.558SerAsp: 2.558 ± 0.029	2.268SerGlu: 2.268 ± 0.031	1.467SerPhe: 1.467 ± 0.028	5.886SerGly: 5.886 ± 0.052	0.972SerHis: 0.972 ± 0.021	1.588SerIle: 1.588 ± 0.028	0.997SerLys: 0.997 ± 0.021	4.553SerLeu: 4.553 ± 0.037	1.095SerMet: 1.095 ± 0.022	0.801SerAsn: 0.801 ± 0.019	3.285SerPro: 3.285 ± 0.034	1.149SerGln: 1.149 ± 0.022	3.88SerArg: 3.88 ± 0.04	2.677SerSer: 2.677 ± 0.039	2.703SerThr: 2.703 ± 0.032	3.865SerVal: 3.865 ± 0.036	0.864SerTrp: 0.864 ± 0.019	1.132SerTyr: 1.132 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	7.976ThrAla: 7.976 ± 0.052	0.469ThrCys: 0.469 ± 0.013	3.004ThrAsp: 3.004 ± 0.031	2.727ThrGlu: 2.727 ± 0.03	1.61ThrPhe: 1.61 ± 0.021	6.579ThrGly: 6.579 ± 0.049	1.069ThrHis: 1.069 ± 0.021	1.955ThrIle: 1.955 ± 0.028	1.043ThrLys: 1.043 ± 0.022	5.437ThrLeu: 5.437 ± 0.045	1.036ThrMet: 1.036 ± 0.022	0.865ThrAsn: 0.865 ± 0.02	3.643ThrPro: 3.643 ± 0.035	1.174ThrGln: 1.174 ± 0.023	4.169ThrArg: 4.169 ± 0.04	2.915ThrSer: 2.915 ± 0.035	3.231ThrThr: 3.231 ± 0.041	5.426ThrVal: 5.426 ± 0.046	0.91ThrTrp: 0.91 ± 0.019	1.188ThrTyr: 1.188 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	10.846ValAla: 10.846 ± 0.061	0.725ValCys: 0.725 ± 0.017	4.721ValAsp: 4.721 ± 0.038	4.482ValGlu: 4.482 ± 0.043	2.437ValPhe: 2.437 ± 0.03	6.311ValGly: 6.311 ± 0.055	1.946ValHis: 1.946 ± 0.027	3.052ValIle: 3.052 ± 0.033	1.63ValLys: 1.63 ± 0.026	9.446ValLeu: 9.446 ± 0.058	1.424ValMet: 1.424 ± 0.025	1.629ValAsn: 1.629 ± 0.025	5.464ValPro: 5.464 ± 0.05	2.161ValGln: 2.161 ± 0.029	7.418ValArg: 7.418 ± 0.061	4.107ValSer: 4.107 ± 0.043	5.329ValThr: 5.329 ± 0.044	7.608ValVal: 7.608 ± 0.063	1.119ValTrp: 1.119 ± 0.022	1.558ValTyr: 1.558 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.676TrpAla: 1.676 ± 0.024	0.164TrpCys: 0.164 ± 0.007	0.889TrpAsp: 0.889 ± 0.02	0.749TrpGlu: 0.749 ± 0.018	0.517TrpPhe: 0.517 ± 0.015	1.027TrpGly: 1.027 ± 0.02	0.381TrpHis: 0.381 ± 0.012	0.571TrpIle: 0.571 ± 0.015	0.349TrpLys: 0.349 ± 0.012	1.779TrpLeu: 1.779 ± 0.027	0.283TrpMet: 0.283 ± 0.01	0.38TrpAsn: 0.38 ± 0.011	0.863TrpPro: 0.863 ± 0.018	0.509TrpGln: 0.509 ± 0.015	1.575TrpArg: 1.575 ± 0.022	0.902TrpSer: 0.902 ± 0.02	1.039TrpThr: 1.039 ± 0.021	0.93TrpVal: 0.93 ± 0.019	0.373TrpTrp: 0.373 ± 0.013	0.334TrpTyr: 0.334 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.49TyrAla: 2.49 ± 0.032	0.187TyrCys: 0.187 ± 0.009	1.339TyrAsp: 1.339 ± 0.027	1.164TyrGlu: 1.164 ± 0.017	0.604TyrPhe: 0.604 ± 0.015	2.131TyrGly: 2.131 ± 0.027	0.406TyrHis: 0.406 ± 0.013	0.53TyrIle: 0.53 ± 0.015	0.393TyrLys: 0.393 ± 0.013	2.131TyrLeu: 2.131 ± 0.028	0.28TyrMet: 0.28 ± 0.01	0.367TyrAsn: 0.367 ± 0.011	1.034TyrPro: 1.034 ± 0.02	0.596TyrGln: 0.596 ± 0.013	1.877TyrArg: 1.877 ± 0.026	0.924TyrSer: 0.924 ± 0.02	1.112TyrThr: 1.112 ± 0.023	1.606TyrVal: 1.606 ± 0.027	0.346TyrTrp: 0.346 ± 0.012	0.442TyrTyr: 0.442 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9016 proteins (2861743 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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