Amino acid dipepetide frequency for Mycobacterium helveticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.787AlaAla: 21.787 ± 0.221	1.137AlaCys: 1.137 ± 0.026	8.364AlaAsp: 8.364 ± 0.08	7.71AlaGlu: 7.71 ± 0.076	3.488AlaPhe: 3.488 ± 0.048	13.135AlaGly: 13.135 ± 0.116	2.737AlaHis: 2.737 ± 0.043	4.971AlaIle: 4.971 ± 0.062	2.79AlaLys: 2.79 ± 0.048	13.171AlaLeu: 13.171 ± 0.121	2.908AlaMet: 2.908 ± 0.044	2.626AlaAsn: 2.626 ± 0.046	6.757AlaPro: 6.757 ± 0.08	4.03AlaGln: 4.03 ± 0.054	9.66AlaArg: 9.66 ± 0.079	5.828AlaSer: 5.828 ± 0.075	7.268AlaThr: 7.268 ± 0.081	11.886AlaVal: 11.886 ± 0.105	1.752AlaTrp: 1.752 ± 0.03	2.395AlaTyr: 2.395 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.186CysAla: 1.186 ± 0.025	0.095CysCys: 0.095 ± 0.007	0.586CysAsp: 0.586 ± 0.018	0.475CysGlu: 0.475 ± 0.016	0.266CysPhe: 0.266 ± 0.012	1.043CysGly: 1.043 ± 0.031	0.208CysHis: 0.208 ± 0.013	0.271CysIle: 0.271 ± 0.014	0.149CysLys: 0.149 ± 0.011	0.692CysLeu: 0.692 ± 0.02	0.148CysMet: 0.148 ± 0.009	0.171CysAsn: 0.171 ± 0.011	0.584CysPro: 0.584 ± 0.02	0.215CysGln: 0.215 ± 0.01	0.731CysArg: 0.731 ± 0.022	0.496CysSer: 0.496 ± 0.018	0.507CysThr: 0.507 ± 0.019	0.696CysVal: 0.696 ± 0.02	0.143CysTrp: 0.143 ± 0.01	0.226CysTyr: 0.226 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.869AspAla: 7.869 ± 0.077	0.496AspCys: 0.496 ± 0.02	4.086AspAsp: 4.086 ± 0.064	3.824AspGlu: 3.824 ± 0.063	1.843AspPhe: 1.843 ± 0.037	5.773AspGly: 5.773 ± 0.069	1.44AspHis: 1.44 ± 0.028	2.476AspIle: 2.476 ± 0.04	1.302AspLys: 1.302 ± 0.033	5.908AspLeu: 5.908 ± 0.054	1.009AspMet: 1.009 ± 0.023	1.167AspAsn: 1.167 ± 0.028	4.478AspPro: 4.478 ± 0.056	1.635AspGln: 1.635 ± 0.033	4.643AspArg: 4.643 ± 0.051	2.575AspSer: 2.575 ± 0.04	3.143AspThr: 3.143 ± 0.044	5.309AspVal: 5.309 ± 0.063	0.964AspTrp: 0.964 ± 0.025	1.458AspTyr: 1.458 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.085GluAla: 6.085 ± 0.066	0.395GluCys: 0.395 ± 0.017	2.284GluAsp: 2.284 ± 0.038	2.427GluGlu: 2.427 ± 0.046	1.812GluPhe: 1.812 ± 0.036	3.185GluGly: 3.185 ± 0.048	1.603GluHis: 1.603 ± 0.034	2.501GluIle: 2.501 ± 0.041	1.225GluLys: 1.225 ± 0.032	6.393GluLeu: 6.393 ± 0.074	1.062GluMet: 1.062 ± 0.024	1.01GluAsn: 1.01 ± 0.027	3.014GluPro: 3.014 ± 0.047	2.089GluGln: 2.089 ± 0.04	4.562GluArg: 4.562 ± 0.06	2.578GluSer: 2.578 ± 0.043	2.487GluThr: 2.487 ± 0.041	4.151GluVal: 4.151 ± 0.055	0.746GluTrp: 0.746 ± 0.023	1.055GluTyr: 1.055 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.087PheAla: 4.087 ± 0.047	0.371PheCys: 0.371 ± 0.015	2.391PheAsp: 2.391 ± 0.043	1.517PheGlu: 1.517 ± 0.034	1.023PhePhe: 1.023 ± 0.027	3.482PheGly: 3.482 ± 0.054	0.655PheHis: 0.655 ± 0.02	0.971PheIle: 0.971 ± 0.025	0.515PheLys: 0.515 ± 0.018	2.524PheLeu: 2.524 ± 0.042	0.467PheMet: 0.467 ± 0.016	0.739PheAsn: 0.739 ± 0.028	1.507PhePro: 1.507 ± 0.03	0.683PheGln: 0.683 ± 0.021	1.754PheArg: 1.754 ± 0.04	1.677PheSer: 1.677 ± 0.032	2.008PheThr: 2.008 ± 0.04	2.548PheVal: 2.548 ± 0.045	0.439PheTrp: 0.439 ± 0.018	0.716PheTyr: 0.716 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	10.897GlyAla: 10.897 ± 0.119	0.908GlyCys: 0.908 ± 0.024	5.114GlyAsp: 5.114 ± 0.053	4.458GlyGlu: 4.458 ± 0.05	3.09GlyPhe: 3.09 ± 0.049	8.429GlyGly: 8.429 ± 0.129	2.213GlyHis: 2.213 ± 0.046	3.937GlyIle: 3.937 ± 0.048	2.292GlyLys: 2.292 ± 0.043	8.859GlyLeu: 8.859 ± 0.102	2.227GlyMet: 2.227 ± 0.04	2.041GlyAsn: 2.041 ± 0.062	5.028GlyPro: 5.028 ± 0.051	2.84GlyGln: 2.84 ± 0.044	7.046GlyArg: 7.046 ± 0.07	5.278GlySer: 5.278 ± 0.066	5.208GlyThr: 5.208 ± 0.068	7.562GlyVal: 7.562 ± 0.084	1.64GlyTrp: 1.64 ± 0.033	2.452GlyTyr: 2.452 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.768HisAla: 2.768 ± 0.047	0.243HisCys: 0.243 ± 0.013	1.426HisAsp: 1.426 ± 0.03	1.06HisGlu: 1.06 ± 0.029	0.722HisPhe: 0.722 ± 0.021	2.288HisGly: 2.288 ± 0.039	0.739HisHis: 0.739 ± 0.024	0.778HisIle: 0.778 ± 0.022	0.425HisLys: 0.425 ± 0.013	2.266HisLeu: 2.266 ± 0.041	0.366HisMet: 0.366 ± 0.016	0.514HisAsn: 0.514 ± 0.018	1.808HisPro: 1.808 ± 0.039	0.672HisGln: 0.672 ± 0.022	2.038HisArg: 2.038 ± 0.039	1.058HisSer: 1.058 ± 0.027	1.253HisThr: 1.253 ± 0.03	1.756HisVal: 1.756 ± 0.036	0.417HisTrp: 0.417 ± 0.016	0.553HisTyr: 0.553 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.968IleAla: 5.968 ± 0.064	0.403IleCys: 0.403 ± 0.016	3.218IleAsp: 3.218 ± 0.048	2.434IleGlu: 2.434 ± 0.041	0.984IlePhe: 0.984 ± 0.024	4.22IleGly: 4.22 ± 0.052	0.808IleHis: 0.808 ± 0.022	1.227IleIle: 1.227 ± 0.031	0.856IleLys: 0.856 ± 0.022	2.821IleLeu: 2.821 ± 0.051	0.594IleMet: 0.594 ± 0.019	1.079IleAsn: 1.079 ± 0.029	2.352IlePro: 2.352 ± 0.041	0.908IleGln: 0.908 ± 0.025	2.593IleArg: 2.593 ± 0.042	2.063IleSer: 2.063 ± 0.037	2.606IleThr: 2.606 ± 0.044	3.474IleVal: 3.474 ± 0.05	0.453IleTrp: 0.453 ± 0.016	0.76IleTyr: 0.76 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	2.593LysAla: 2.593 ± 0.048	0.117LysCys: 0.117 ± 0.01	1.07LysAsp: 1.07 ± 0.03	0.887LysGlu: 0.887 ± 0.024	0.61LysPhe: 0.61 ± 0.02	1.477LysGly: 1.477 ± 0.032	0.48LysHis: 0.48 ± 0.018	1.003LysIle: 1.003 ± 0.023	0.565LysLys: 0.565 ± 0.022	2.144LysLeu: 2.144 ± 0.042	0.475LysMet: 0.475 ± 0.018	0.522LysAsn: 0.522 ± 0.019	1.38LysPro: 1.38 ± 0.03	0.661LysGln: 0.661 ± 0.023	1.547LysArg: 1.547 ± 0.034	1.142LysSer: 1.142 ± 0.026	1.335LysThr: 1.335 ± 0.03	1.87LysVal: 1.87 ± 0.038	0.304LysTrp: 0.304 ± 0.014	0.447LysTyr: 0.447 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.427LeuAla: 14.427 ± 0.114	0.89LeuCys: 0.89 ± 0.022	6.485LeuAsp: 6.485 ± 0.071	4.28LeuGlu: 4.28 ± 0.057	2.678LeuPhe: 2.678 ± 0.042	8.919LeuGly: 8.919 ± 0.087	2.176LeuHis: 2.176 ± 0.041	3.821LeuIle: 3.821 ± 0.051	1.721LeuLys: 1.721 ± 0.037	9.636LeuLeu: 9.636 ± 0.104	1.692LeuMet: 1.692 ± 0.034	2.12LeuAsn: 2.12 ± 0.055	5.957LeuPro: 5.957 ± 0.062	2.587LeuGln: 2.587 ± 0.045	8.163LeuArg: 8.163 ± 0.08	5.589LeuSer: 5.589 ± 0.07	6.17LeuThr: 6.17 ± 0.068	8.318LeuVal: 8.318 ± 0.065	1.18LeuTrp: 1.18 ± 0.028	1.642LeuTyr: 1.642 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.631MetAla: 2.631 ± 0.037	0.197MetCys: 0.197 ± 0.012	0.9MetAsp: 0.9 ± 0.022	0.701MetGlu: 0.701 ± 0.022	0.652MetPhe: 0.652 ± 0.02	1.459MetGly: 1.459 ± 0.036	0.435MetHis: 0.435 ± 0.015	0.846MetIle: 0.846 ± 0.022	0.461MetLys: 0.461 ± 0.017	2.103MetLeu: 2.103 ± 0.037	0.397MetMet: 0.397 ± 0.016	0.48MetAsn: 0.48 ± 0.014	1.255MetPro: 1.255 ± 0.03	0.525MetGln: 0.525 ± 0.017	1.597MetArg: 1.597 ± 0.029	1.503MetSer: 1.503 ± 0.031	1.732MetThr: 1.732 ± 0.034	1.593MetVal: 1.593 ± 0.033	0.279MetTrp: 0.279 ± 0.013	0.373MetTyr: 0.373 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.712AsnAla: 2.712 ± 0.043	0.188AsnCys: 0.188 ± 0.01	1.194AsnAsp: 1.194 ± 0.027	0.931AsnGlu: 0.931 ± 0.027	0.705AsnPhe: 0.705 ± 0.022	2.098AsnGly: 2.098 ± 0.041	0.467AsnHis: 0.467 ± 0.018	0.893AsnIle: 0.893 ± 0.024	0.459AsnLys: 0.459 ± 0.019	2.173AsnLeu: 2.173 ± 0.043	0.427AsnMet: 0.427 ± 0.017	0.556AsnAsn: 0.556 ± 0.021	1.747AsnPro: 1.747 ± 0.034	0.638AsnGln: 0.638 ± 0.02	1.552AsnArg: 1.552 ± 0.032	1.119AsnSer: 1.119 ± 0.036	1.267AsnThr: 1.267 ± 0.035	1.716AsnVal: 1.716 ± 0.037	0.39AsnTrp: 0.39 ± 0.014	0.58AsnTyr: 0.58 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	8.075ProAla: 8.075 ± 0.085	0.335ProCys: 0.335 ± 0.016	4.524ProAsp: 4.524 ± 0.052	3.664ProGlu: 3.664 ± 0.049	1.613ProPhe: 1.613 ± 0.029	6.46ProGly: 6.46 ± 0.079	1.289ProHis: 1.289 ± 0.027	2.052ProIle: 2.052 ± 0.032	1.233ProLys: 1.233 ± 0.031	5.035ProLeu: 5.035 ± 0.053	1.227ProMet: 1.227 ± 0.026	1.235ProAsn: 1.235 ± 0.029	4.194ProPro: 4.194 ± 0.116	1.867ProGln: 1.867 ± 0.032	3.901ProArg: 3.901 ± 0.047	2.96ProSer: 2.96 ± 0.043	3.3ProThr: 3.3 ± 0.051	5.103ProVal: 5.103 ± 0.064	0.883ProTrp: 0.883 ± 0.025	1.187ProTyr: 1.187 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.657GlnAla: 3.657 ± 0.046	0.239GlnCys: 0.239 ± 0.013	1.199GlnAsp: 1.199 ± 0.028	1.042GlnGlu: 1.042 ± 0.026	0.944GlnPhe: 0.944 ± 0.026	2.004GlnGly: 2.004 ± 0.04	0.784GlnHis: 0.784 ± 0.019	1.405GlnIle: 1.405 ± 0.032	0.584GlnLys: 0.584 ± 0.021	3.509GlnLeu: 3.509 ± 0.056	0.658GlnMet: 0.658 ± 0.018	0.589GlnAsn: 0.589 ± 0.02	1.84GlnPro: 1.84 ± 0.04	1.237GlnGln: 1.237 ± 0.032	2.909GlnArg: 2.909 ± 0.048	1.434GlnSer: 1.434 ± 0.031	1.503GlnThr: 1.503 ± 0.032	2.538GlnVal: 2.538 ± 0.045	0.522GlnTrp: 0.522 ± 0.02	0.638GlnTyr: 0.638 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.299ArgAla: 9.299 ± 0.095	0.719ArgCys: 0.719 ± 0.025	4.454ArgAsp: 4.454 ± 0.056	3.86ArgGlu: 3.86 ± 0.06	2.545ArgPhe: 2.545 ± 0.037	5.693ArgGly: 5.693 ± 0.065	2.122ArgHis: 2.122 ± 0.036	3.444ArgIle: 3.444 ± 0.05	1.582ArgLys: 1.582 ± 0.035	8.158ArgLeu: 8.158 ± 0.082	1.887ArgMet: 1.887 ± 0.035	1.648ArgAsn: 1.648 ± 0.031	4.46ArgPro: 4.46 ± 0.054	2.421ArgGln: 2.421 ± 0.037	7.679ArgArg: 7.679 ± 0.092	4.052ArgSer: 4.052 ± 0.049	4.055ArgThr: 4.055 ± 0.049	5.951ArgVal: 5.951 ± 0.064	1.451ArgTrp: 1.451 ± 0.034	1.991ArgTyr: 1.991 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.938SerAla: 6.938 ± 0.075	0.451SerCys: 0.451 ± 0.016	2.881SerAsp: 2.881 ± 0.048	2.319SerGlu: 2.319 ± 0.042	1.594SerPhe: 1.594 ± 0.034	5.647SerGly: 5.647 ± 0.083	1.079SerHis: 1.079 ± 0.026	1.972SerIle: 1.972 ± 0.036	1.042SerLys: 1.042 ± 0.025	4.788SerLeu: 4.788 ± 0.069	1.211SerMet: 1.211 ± 0.026	1.089SerAsn: 1.089 ± 0.023	3.191SerPro: 3.191 ± 0.046	1.381SerGln: 1.381 ± 0.03	3.795SerArg: 3.795 ± 0.052	3.025SerSer: 3.025 ± 0.05	3.097SerThr: 3.097 ± 0.048	4.42SerVal: 4.42 ± 0.052	0.886SerTrp: 0.886 ± 0.023	1.264SerTyr: 1.264 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	7.728ThrAla: 7.728 ± 0.076	0.442ThrCys: 0.442 ± 0.018	3.405ThrAsp: 3.405 ± 0.044	2.912ThrGlu: 2.912 ± 0.049	1.665ThrPhe: 1.665 ± 0.033	5.96ThrGly: 5.96 ± 0.078	1.166ThrHis: 1.166 ± 0.029	2.339ThrIle: 2.339 ± 0.041	1.129ThrLys: 1.129 ± 0.028	5.474ThrLeu: 5.474 ± 0.06	1.213ThrMet: 1.213 ± 0.026	1.257ThrAsn: 1.257 ± 0.031	3.774ThrPro: 3.774 ± 0.049	1.438ThrGln: 1.438 ± 0.032	3.752ThrArg: 3.752 ± 0.046	2.939ThrSer: 2.939 ± 0.039	3.642ThrThr: 3.642 ± 0.062	5.572ThrVal: 5.572 ± 0.069	0.822ThrTrp: 0.822 ± 0.021	1.27ThrTyr: 1.27 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	11.607ValAla: 11.607 ± 0.116	0.833ValCys: 0.833 ± 0.021	5.637ValAsp: 5.637 ± 0.072	4.415ValGlu: 4.415 ± 0.059	2.571ValPhe: 2.571 ± 0.041	7.124ValGly: 7.124 ± 0.08	1.9ValHis: 1.9 ± 0.033	3.708ValIle: 3.708 ± 0.051	1.653ValLys: 1.653 ± 0.034	8.645ValLeu: 8.645 ± 0.08	1.544ValMet: 1.544 ± 0.027	2.081ValAsn: 2.081 ± 0.034	4.733ValPro: 4.733 ± 0.051	2.079ValGln: 2.079 ± 0.037	6.219ValArg: 6.219 ± 0.064	4.658ValSer: 4.658 ± 0.054	5.309ValThr: 5.309 ± 0.065	8.489ValVal: 8.489 ± 0.083	1.049ValTrp: 1.049 ± 0.023	1.565ValTyr: 1.565 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.617TrpAla: 1.617 ± 0.031	0.182TrpCys: 0.182 ± 0.012	0.795TrpAsp: 0.795 ± 0.019	0.675TrpGlu: 0.675 ± 0.022	0.491TrpPhe: 0.491 ± 0.02	1.022TrpGly: 1.022 ± 0.026	0.365TrpHis: 0.365 ± 0.015	0.601TrpIle: 0.601 ± 0.021	0.308TrpLys: 0.308 ± 0.015	1.789TrpLeu: 1.789 ± 0.037	0.329TrpMet: 0.329 ± 0.014	0.405TrpAsn: 0.405 ± 0.015	0.83TrpPro: 0.83 ± 0.027	0.625TrpGln: 0.625 ± 0.019	1.357TrpArg: 1.357 ± 0.026	0.955TrpSer: 0.955 ± 0.023	0.851TrpThr: 0.851 ± 0.022	1.163TrpVal: 1.163 ± 0.028	0.344TrpTrp: 0.344 ± 0.016	0.331TrpTyr: 0.331 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.471TyrAla: 2.471 ± 0.036	0.245TyrCys: 0.245 ± 0.014	1.386TyrAsp: 1.386 ± 0.031	1.073TyrGlu: 1.073 ± 0.024	0.776TyrPhe: 0.776 ± 0.021	2.102TyrGly: 2.102 ± 0.043	0.483TyrHis: 0.483 ± 0.017	0.605TyrIle: 0.605 ± 0.02	0.34TyrLys: 0.34 ± 0.015	2.362TyrLeu: 2.362 ± 0.038	0.274TyrMet: 0.274 ± 0.012	0.485TyrAsn: 0.485 ± 0.017	1.248TyrPro: 1.248 ± 0.029	0.704TyrGln: 0.704 ± 0.023	1.973TyrArg: 1.973 ± 0.035	1.143TyrSer: 1.143 ± 0.025	1.158TyrThr: 1.158 ± 0.028	1.705TyrVal: 1.705 ± 0.035	0.371TyrTrp: 0.371 ± 0.016	0.508TyrTyr: 0.508 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5147 proteins (1625944 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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