Capybara microvirus Cap1_SP_163
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVQ4|A0A4V1FVQ4_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_163 OX=2585399 PE=4 SV=1
MM1 pKa = 7.16 KK2 pKa = 10.21 HH3 pKa = 6.18 SFSIYY8 pKa = 10.13 AFQFCEE14 pKa = 3.77 RR15 pKa = 11.84 GVWKK19 pKa = 10.17 WSLPFPAVNSSAARR33 pKa = 11.84 EE34 pKa = 4.12 AVKK37 pKa = 10.81 SLFSEE42 pKa = 4.07 IGSVLLYY49 pKa = 10.68 EE50 pKa = 4.24 IGEE53 pKa = 4.2 YY54 pKa = 9.74 QDD56 pKa = 5.72 GIITPLEE63 pKa = 4.51 HH64 pKa = 8.04 IEE66 pKa = 4.18 VEE68 pKa = 4.33 LL69 pKa = 4.81
Molecular weight: 7.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.887
IPC2_protein 5.054
IPC_protein 4.838
Toseland 4.774
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.609
Rodwell 4.724
Grimsley 4.698
Solomon 4.774
Lehninger 4.736
Nozaki 4.902
DTASelect 4.927
Thurlkill 4.762
EMBOSS 4.635
Sillero 4.991
Patrickios 3.605
IPC_peptide 4.8
IPC2_peptide 4.991
IPC2.peptide.svr19 4.944
Protein with the highest isoelectric point:
>tr|A0A4P8W488|A0A4P8W488_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_163 OX=2585399 PE=4 SV=1
MM1 pKa = 8.22 DD2 pKa = 4.17 ILGRR6 pKa = 11.84 ISLACLFVLVLLTGIFLIMRR26 pKa = 11.84 GCSHH30 pKa = 6.62 NPHH33 pKa = 5.93 NRR35 pKa = 11.84 HH36 pKa = 4.42 YY37 pKa = 9.4 TKK39 pKa = 10.6 SLSQTGVGVSSCVRR53 pKa = 11.84 SEE55 pKa = 3.87 CAYY58 pKa = 10.0 RR59 pKa = 11.84 AGRR62 pKa = 11.84 PRR64 pKa = 11.84 DD65 pKa = 3.42 ARR67 pKa = 11.84 ASEE70 pKa = 4.12 GHH72 pKa = 6.64 ASRR75 pKa = 11.84 VLARR79 pKa = 11.84 VARR82 pKa = 11.84 NINISTRR89 pKa = 11.84 II90 pKa = 3.53
Molecular weight: 9.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.237
IPC2_protein 9.794
IPC_protein 11.082
Toseland 11.067
ProMoST 11.491
Dawson 11.111
Bjellqvist 11.067
Wikipedia 11.55
Rodwell 10.833
Grimsley 11.169
Solomon 11.535
Lehninger 11.447
Nozaki 11.096
DTASelect 11.067
Thurlkill 11.082
EMBOSS 11.55
Sillero 11.096
Patrickios 10.643
IPC_peptide 11.535
IPC2_peptide 10.599
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1542
69
556
257.0
28.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.042 ± 1.652
2.01 ± 0.699
6.031 ± 0.642
4.994 ± 0.645
4.604 ± 0.521
7.652 ± 0.665
1.751 ± 0.624
4.929 ± 0.632
4.28 ± 0.508
7.912 ± 0.231
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.14 ± 0.132
4.929 ± 0.652
4.345 ± 0.888
4.54 ± 0.965
6.68 ± 1.199
9.014 ± 1.103
4.54 ± 0.902
5.577 ± 0.476
1.621 ± 0.278
4.41 ± 0.618
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here