Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Morganellaceae; Photorhabdus; Photorhabdus laumondii; Photorhabdus laumondii subsp. laumondii

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7N7X7|Q7N7X7_PHOLL 4-HPA 3-monooxygenase small component OS=Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) OX=243265 GN=hpaC PE=3 SV=1
MM1 pKa = 7.2TGQYY5 pKa = 10.16PFLDD9 pKa = 3.8ILMHH13 pKa = 7.22AYY15 pKa = 10.06FNQDD19 pKa = 2.2FDD21 pKa = 4.74IISGPEE27 pKa = 3.9LDD29 pKa = 5.69DD30 pKa = 5.14VINDD34 pKa = 3.96FLNDD38 pKa = 3.26ASQGMRR44 pKa = 11.84KK45 pKa = 9.77GLIEE49 pKa = 5.07EE50 pKa = 4.32INDD53 pKa = 4.67LIDD56 pKa = 3.56SSEE59 pKa = 4.15DD60 pKa = 3.36VEE62 pKa = 4.84STFDD66 pKa = 3.58YY67 pKa = 10.28HH68 pKa = 7.63YY69 pKa = 10.85HH70 pKa = 6.87DD71 pKa = 5.64ADD73 pKa = 3.79VLPEE77 pKa = 4.13GWGMTALEE85 pKa = 4.38FLTHH89 pKa = 6.38VSNKK93 pKa = 9.66SQDD96 pKa = 3.62YY97 pKa = 10.51LNEE100 pKa = 3.81HH101 pKa = 6.05TEE103 pKa = 3.78QDD105 pKa = 3.48EE106 pKa = 4.14

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7MY83|Q7MY83_PHOLL WblI protein OS=Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) OX=243265 GN=wblI PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4556

0

4556

1496065

30

16367

328.4

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.992 ± 0.044

1.094 ± 0.015

5.24 ± 0.028

5.907 ± 0.037

3.922 ± 0.032

6.842 ± 0.046

2.353 ± 0.017

6.938 ± 0.049

5.157 ± 0.052

10.537 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.02

4.683 ± 0.041

4.209 ± 0.037

4.71 ± 0.039

5.084 ± 0.033

6.497 ± 0.036

5.419 ± 0.042

6.381 ± 0.036

1.343 ± 0.016

3.258 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski