Streptococcus sp. oral taxon 056 str. F0418
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1957 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9HHN6|F9HHN6_9STRE Replication initiation factor OS=Streptococcus sp. oral taxon 056 str. F0418 OX=904294 GN=HMPREF9182_0580 PE=3 SV=1
MM1 pKa = 8.08 DD2 pKa = 4.11 ICSDD6 pKa = 3.56 YY7 pKa = 11.25 LYY9 pKa = 11.29 GKK11 pKa = 10.43 GFLSLPEE18 pKa = 4.7 SIQDD22 pKa = 3.66 AVDD25 pKa = 3.62 EE26 pKa = 4.69 YY27 pKa = 11.13 FDD29 pKa = 3.4 GWEE32 pKa = 3.93 VEE34 pKa = 4.25 QYY36 pKa = 11.89 GNGNPDD42 pKa = 3.88 DD43 pKa = 3.85 MWVNHH48 pKa = 5.19 YY49 pKa = 10.62 VSYY52 pKa = 10.79 DD53 pKa = 3.62 SKK55 pKa = 11.37 EE56 pKa = 3.97 LLIDD60 pKa = 4.48 TINMLSSQEE69 pKa = 3.94 YY70 pKa = 9.56 QEE72 pKa = 4.55 LLEE75 pKa = 4.13 EE76 pKa = 4.26 EE77 pKa = 4.97 RR78 pKa = 11.84 LEE80 pKa = 4.04 EE81 pKa = 4.25 WIEE84 pKa = 3.85 ANRR87 pKa = 11.84 EE88 pKa = 3.98 EE89 pKa = 4.39 IEE91 pKa = 3.87 EE92 pKa = 4.66 RR93 pKa = 11.84 INDD96 pKa = 3.68 SYY98 pKa = 12.14 SFLGYY103 pKa = 10.35 ADD105 pKa = 4.49 KK106 pKa = 10.47 EE107 pKa = 4.13 WHH109 pKa = 6.05 VFVV112 pKa = 5.01
Molecular weight: 13.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.579
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 3.986
Thurlkill 3.617
EMBOSS 3.63
Sillero 3.872
Patrickios 0.477
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|F9HFS6|F9HFS6_9STRE Autoinducer 2 import ATP-binding protein LsrA OS=Streptococcus sp. oral taxon 056 str. F0418 OX=904294 GN=lsrA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.18 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.78 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLAAA44 pKa = 4.31
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1957
0
1957
560762
37
3829
286.5
32.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.446 ± 0.083
0.557 ± 0.017
5.516 ± 0.047
7.047 ± 0.073
4.716 ± 0.046
6.436 ± 0.049
1.941 ± 0.029
7.349 ± 0.059
6.765 ± 0.058
10.25 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.028
4.403 ± 0.046
3.244 ± 0.034
4.211 ± 0.043
4.11 ± 0.046
6.391 ± 0.137
5.515 ± 0.068
6.847 ± 0.055
0.913 ± 0.022
3.845 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here