Streptococcus sp. oral taxon 056 str. F0418 
Average proteome isoelectric point is 6.38 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 1957 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|F9HHN6|F9HHN6_9STRE Replication initiation factor OS=Streptococcus sp. oral taxon 056 str. F0418 OX=904294 GN=HMPREF9182_0580 PE=3 SV=1 
MM1 pKa = 8.08  DD2 pKa = 4.11  ICSDD6 pKa = 3.56  YY7 pKa = 11.25  LYY9 pKa = 11.29  GKK11 pKa = 10.43  GFLSLPEE18 pKa = 4.7  SIQDD22 pKa = 3.66  AVDD25 pKa = 3.62  EE26 pKa = 4.69  YY27 pKa = 11.13  FDD29 pKa = 3.4  GWEE32 pKa = 3.93  VEE34 pKa = 4.25  QYY36 pKa = 11.89  GNGNPDD42 pKa = 3.88  DD43 pKa = 3.85  MWVNHH48 pKa = 5.19  YY49 pKa = 10.62  VSYY52 pKa = 10.79  DD53 pKa = 3.62  SKK55 pKa = 11.37  EE56 pKa = 3.97  LLIDD60 pKa = 4.48  TINMLSSQEE69 pKa = 3.94  YY70 pKa = 9.56  QEE72 pKa = 4.55  LLEE75 pKa = 4.13  EE76 pKa = 4.26  EE77 pKa = 4.97  RR78 pKa = 11.84  LEE80 pKa = 4.04  EE81 pKa = 4.25  WIEE84 pKa = 3.85  ANRR87 pKa = 11.84  EE88 pKa = 3.98  EE89 pKa = 4.39  IEE91 pKa = 3.87  EE92 pKa = 4.66  RR93 pKa = 11.84  INDD96 pKa = 3.68  SYY98 pKa = 12.14  SFLGYY103 pKa = 10.35  ADD105 pKa = 4.49  KK106 pKa = 10.47  EE107 pKa = 4.13  WHH109 pKa = 6.05  VFVV112 pKa = 5.01   
 Molecular weight: 13.38 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.799 
IPC2_protein 3.821 
IPC_protein 3.757 
Toseland    3.579 
ProMoST     3.897 
Dawson      3.732 
Bjellqvist  3.884 
Wikipedia   3.617 
Rodwell     3.592 
Grimsley    3.49 
Solomon     3.719 
Lehninger   3.668 
Nozaki      3.846 
DTASelect   3.986 
Thurlkill   3.617 
EMBOSS      3.63 
Sillero     3.872 
Patrickios  0.477 
IPC_peptide 3.719 
IPC2_peptide  3.859 
IPC2.peptide.svr19  3.798 
 Protein with the highest isoelectric point: 
>tr|F9HFS6|F9HFS6_9STRE Autoinducer 2 import ATP-binding protein LsrA OS=Streptococcus sp. oral taxon 056 str. F0418 OX=904294 GN=lsrA PE=3 SV=1 
MM1 pKa = 7.35  KK2 pKa = 9.43  RR3 pKa = 11.84  TYY5 pKa = 10.07  QPSKK9 pKa = 9.18  IRR11 pKa = 11.84  RR12 pKa = 11.84  ARR14 pKa = 11.84  KK15 pKa = 8.35  HH16 pKa = 4.78  GFRR19 pKa = 11.84  NRR21 pKa = 11.84  MSTKK25 pKa = 9.22  NGRR28 pKa = 11.84  RR29 pKa = 11.84  VLAARR34 pKa = 11.84  RR35 pKa = 11.84  RR36 pKa = 11.84  KK37 pKa = 8.87  GRR39 pKa = 11.84  KK40 pKa = 8.75  VLAAA44 pKa = 4.31   
 Molecular weight: 5.27 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.449 
IPC2_protein 11.155 
IPC_protein 12.544 
Toseland    12.705 
ProMoST     13.203 
Dawson      12.705 
Bjellqvist  12.705 
Wikipedia   13.188 
Rodwell     12.398 
Grimsley    12.749 
Solomon     13.203 
Lehninger   13.1 
Nozaki      12.705 
DTASelect   12.705 
Thurlkill   12.705 
EMBOSS      13.203 
Sillero     12.705 
Patrickios  12.135 
IPC_peptide 13.203 
IPC2_peptide  12.193 
IPC2.peptide.svr19  9.082 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        1957 
 
        
        0
 
        
        1957 
         
        560762
 
        37
 
        3829
 
        286.5
 
        32.21
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        7.446 ± 0.083
0.557 ± 0.017
 
        5.516 ± 0.047
7.047 ± 0.073
 
        4.716 ± 0.046
6.436 ± 0.049
 
        1.941 ± 0.029
7.349 ± 0.059
       
        6.765 ± 0.058
10.25 ± 0.096
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.497 ± 0.028
4.403 ± 0.046
 
        3.244 ± 0.034
4.211 ± 0.043
 
        4.11 ± 0.046
6.391 ± 0.137
 
        5.515 ± 0.068
6.847 ± 0.055
       
        0.913 ± 0.022
3.845 ± 0.047
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here