Bellilinea caldifistulae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; Bellilinea

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2990 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P6XND8|A0A0P6XND8_9CHLR Glycos_transf_1 domain-containing protein OS=Bellilinea caldifistulae OX=360411 GN=AC812_02115 PE=4 SV=1
MM1 pKa = 6.6TTITLWEE8 pKa = 4.28PEE10 pKa = 4.12EE11 pKa = 4.17RR12 pKa = 11.84PAYY15 pKa = 8.78ILHH18 pKa = 6.1YY19 pKa = 10.69GDD21 pKa = 4.23VEE23 pKa = 4.74VVGPTLAADD32 pKa = 3.81EE33 pKa = 5.33VICDD37 pKa = 3.77MCLSDD42 pKa = 5.43LPLRR46 pKa = 11.84PVPVVDD52 pKa = 5.3GDD54 pKa = 4.38ALCLEE59 pKa = 5.15CLTKK63 pKa = 10.37VVPDD67 pKa = 3.56WQSQVTPLLEE77 pKa = 4.96LIWRR81 pKa = 11.84TQISEE86 pKa = 4.28FQASEE91 pKa = 3.86

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P6X0T5|A0A0P6X0T5_9CHLR 50S ribosomal protein L27 OS=Bellilinea caldifistulae OX=360411 GN=rpmA PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84WRR4 pKa = 11.84ARR6 pKa = 11.84WRR8 pKa = 11.84SRR10 pKa = 11.84KK11 pKa = 8.79RR12 pKa = 11.84RR13 pKa = 11.84PWGSRR18 pKa = 11.84RR19 pKa = 11.84ARR21 pKa = 11.84CSRR24 pKa = 11.84RR25 pKa = 11.84GNGGWQRR32 pKa = 11.84GWRR35 pKa = 11.84SRR37 pKa = 11.84EE38 pKa = 3.5GRR40 pKa = 11.84PRR42 pKa = 11.84RR43 pKa = 11.84ARR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84CSRR51 pKa = 11.84RR52 pKa = 11.84GNGGRR57 pKa = 11.84QRR59 pKa = 11.84GWRR62 pKa = 11.84SRR64 pKa = 11.84EE65 pKa = 3.5GRR67 pKa = 11.84PRR69 pKa = 11.84RR70 pKa = 11.84ARR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84PRR78 pKa = 11.84WGVGCHH84 pKa = 5.11SGGWGEE90 pKa = 3.73AARR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 3.52

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2990

0

2990

974906

48

2851

326.1

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.059 ± 0.046

0.793 ± 0.014

4.618 ± 0.033

6.447 ± 0.056

4.131 ± 0.032

7.486 ± 0.041

1.867 ± 0.022

6.321 ± 0.045

3.518 ± 0.036

11.479 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.02

3.428 ± 0.028

5.409 ± 0.035

4.373 ± 0.034

6.426 ± 0.046

5.493 ± 0.035

5.074 ± 0.039

7.261 ± 0.038

1.609 ± 0.018

2.965 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski