Amino acid dipepetide frequency for Orenia metallireducens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.021AlaAla: 4.021 ± 0.08	0.452AlaCys: 0.452 ± 0.024	3.559AlaAsp: 3.559 ± 0.077	4.945AlaGlu: 4.945 ± 0.088	2.176AlaPhe: 2.176 ± 0.055	4.789AlaGly: 4.789 ± 0.083	0.916AlaHis: 0.916 ± 0.034	5.816AlaIle: 5.816 ± 0.089	5.576AlaLys: 5.576 ± 0.082	5.969AlaLeu: 5.969 ± 0.094	1.452AlaMet: 1.452 ± 0.041	2.913AlaAsn: 2.913 ± 0.063	1.622AlaPro: 1.622 ± 0.046	2.073AlaGln: 2.073 ± 0.051	2.448AlaArg: 2.448 ± 0.06	3.11AlaSer: 3.11 ± 0.07	3.252AlaThr: 3.252 ± 0.069	4.149AlaVal: 4.149 ± 0.072	0.41AlaTrp: 0.41 ± 0.021	2.042AlaTyr: 2.042 ± 0.045	0.001AlaXaa: 0.001 ± 0.001
Cys	0.378CysAla: 0.378 ± 0.024	0.104CysCys: 0.104 ± 0.012	0.388CysAsp: 0.388 ± 0.024	0.483CysGlu: 0.483 ± 0.024	0.227CysPhe: 0.227 ± 0.016	0.732CysGly: 0.732 ± 0.029	0.136CysHis: 0.136 ± 0.013	0.573CysIle: 0.573 ± 0.024	0.506CysLys: 0.506 ± 0.026	0.575CysLeu: 0.575 ± 0.023	0.143CysMet: 0.143 ± 0.013	0.379CysAsn: 0.379 ± 0.023	0.422CysPro: 0.422 ± 0.025	0.294CysGln: 0.294 ± 0.02	0.309CysArg: 0.309 ± 0.018	0.493CysSer: 0.493 ± 0.023	0.323CysThr: 0.323 ± 0.019	0.376CysVal: 0.376 ± 0.024	0.063CysTrp: 0.063 ± 0.009	0.267CysTyr: 0.267 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	2.795AspAla: 2.795 ± 0.072	0.443AspCys: 0.443 ± 0.024	3.15AspAsp: 3.15 ± 0.057	4.346AspGlu: 4.346 ± 0.066	2.848AspPhe: 2.848 ± 0.057	3.575AspGly: 3.575 ± 0.083	0.8AspHis: 0.8 ± 0.03	6.117AspIle: 6.117 ± 0.098	5.766AspLys: 5.766 ± 0.088	6.19AspLeu: 6.19 ± 0.08	1.284AspMet: 1.284 ± 0.041	3.761AspAsn: 3.761 ± 0.073	1.569AspPro: 1.569 ± 0.039	1.523AspGln: 1.523 ± 0.041	2.462AspArg: 2.462 ± 0.056	3.179AspSer: 3.179 ± 0.055	2.454AspThr: 2.454 ± 0.051	3.284AspVal: 3.284 ± 0.069	0.545AspTrp: 0.545 ± 0.029	3.13AspTyr: 3.13 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	5.094GluAla: 5.094 ± 0.093	0.479GluCys: 0.479 ± 0.029	5.276GluAsp: 5.276 ± 0.086	9.189GluGlu: 9.189 ± 0.148	3.171GluPhe: 3.171 ± 0.066	5.134GluGly: 5.134 ± 0.078	1.122GluHis: 1.122 ± 0.037	7.981GluIle: 7.981 ± 0.091	6.016GluLys: 6.016 ± 0.099	8.945GluLeu: 8.945 ± 0.116	2.042GluMet: 2.042 ± 0.047	3.873GluAsn: 3.873 ± 0.067	1.528GluPro: 1.528 ± 0.046	2.283GluGln: 2.283 ± 0.051	3.303GluArg: 3.303 ± 0.065	3.701GluSer: 3.701 ± 0.065	2.746GluThr: 2.746 ± 0.063	6.64GluVal: 6.64 ± 0.098	0.578GluTrp: 0.578 ± 0.026	3.114GluTyr: 3.114 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.577PheAla: 2.577 ± 0.058	0.349PheCys: 0.349 ± 0.019	2.503PheAsp: 2.503 ± 0.052	2.632PheGlu: 2.632 ± 0.061	1.76PhePhe: 1.76 ± 0.052	2.927PheGly: 2.927 ± 0.064	0.542PheHis: 0.542 ± 0.023	3.743PheIle: 3.743 ± 0.087	3.411PheLys: 3.411 ± 0.067	3.671PheLeu: 3.671 ± 0.078	0.959PheMet: 0.959 ± 0.036	2.592PheAsn: 2.592 ± 0.052	1.246PhePro: 1.246 ± 0.035	0.934PheGln: 0.934 ± 0.032	1.288PheArg: 1.288 ± 0.039	2.841PheSer: 2.841 ± 0.062	2.152PheThr: 2.152 ± 0.051	2.28PheVal: 2.28 ± 0.051	0.381PheTrp: 0.381 ± 0.02	1.613PheTyr: 1.613 ± 0.053	0.001PheXaa: 0.001 ± 0.001
Gly	4.313GlyAla: 4.313 ± 0.094	0.659GlyCys: 0.659 ± 0.032	3.777GlyAsp: 3.777 ± 0.066	5.083GlyGlu: 5.083 ± 0.074	2.933GlyPhe: 2.933 ± 0.063	4.516GlyGly: 4.516 ± 0.093	1.164GlyHis: 1.164 ± 0.037	6.507GlyIle: 6.507 ± 0.093	4.777GlyLys: 4.777 ± 0.084	6.821GlyLeu: 6.821 ± 0.111	1.759GlyMet: 1.759 ± 0.045	2.94GlyAsn: 2.94 ± 0.067	1.394GlyPro: 1.394 ± 0.047	2.052GlyGln: 2.052 ± 0.046	2.842GlyArg: 2.842 ± 0.066	3.59GlySer: 3.59 ± 0.074	3.306GlyThr: 3.306 ± 0.074	5.256GlyVal: 5.256 ± 0.072	0.56GlyTrp: 0.56 ± 0.025	3.084GlyTyr: 3.084 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.767HisAla: 0.767 ± 0.033	0.156HisCys: 0.156 ± 0.012	0.745HisAsp: 0.745 ± 0.035	0.781HisGlu: 0.781 ± 0.031	0.65HisPhe: 0.65 ± 0.028	1.086HisGly: 1.086 ± 0.034	0.4HisHis: 0.4 ± 0.023	1.198HisIle: 1.198 ± 0.035	0.929HisLys: 0.929 ± 0.03	1.456HisLeu: 1.456 ± 0.039	0.315HisMet: 0.315 ± 0.015	0.804HisAsn: 0.804 ± 0.032	0.824HisPro: 0.824 ± 0.03	0.703HisGln: 0.703 ± 0.028	0.606HisArg: 0.606 ± 0.028	0.906HisSer: 0.906 ± 0.031	0.718HisThr: 0.718 ± 0.028	0.678HisVal: 0.678 ± 0.027	0.139HisTrp: 0.139 ± 0.015	0.616HisTyr: 0.616 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.85IleAla: 5.85 ± 0.088	0.674IleCys: 0.674 ± 0.028	6.038IleAsp: 6.038 ± 0.083	7.36IleGlu: 7.36 ± 0.087	3.805IlePhe: 3.805 ± 0.083	6.101IleGly: 6.101 ± 0.086	1.188IleHis: 1.188 ± 0.032	8.934IleIle: 8.934 ± 0.138	8.037IleLys: 8.037 ± 0.107	8.574IleLeu: 8.574 ± 0.125	1.812IleMet: 1.812 ± 0.044	5.382IleAsn: 5.382 ± 0.091	3.149IlePro: 3.149 ± 0.066	2.052IleGln: 2.052 ± 0.053	3.233IleArg: 3.233 ± 0.062	6.176IleSer: 6.176 ± 0.093	4.514IleThr: 4.514 ± 0.084	5.711IleVal: 5.711 ± 0.082	0.507IleTrp: 0.507 ± 0.026	3.124IleTyr: 3.124 ± 0.071	0.0IleXaa: 0.0 ± 0.0
Lys	4.899LysAla: 4.899 ± 0.082	0.433LysCys: 0.433 ± 0.023	5.368LysAsp: 5.368 ± 0.09	9.362LysGlu: 9.362 ± 0.123	2.823LysPhe: 2.823 ± 0.053	4.957LysGly: 4.957 ± 0.088	0.919LysHis: 0.919 ± 0.032	6.984LysIle: 6.984 ± 0.095	6.194LysLys: 6.194 ± 0.101	8.346LysLeu: 8.346 ± 0.113	1.928LysMet: 1.928 ± 0.046	3.858LysAsn: 3.858 ± 0.075	1.827LysPro: 1.827 ± 0.043	2.211LysGln: 2.211 ± 0.055	3.586LysArg: 3.586 ± 0.067	4.244LysSer: 4.244 ± 0.069	3.078LysThr: 3.078 ± 0.062	6.563LysVal: 6.563 ± 0.102	0.542LysTrp: 0.542 ± 0.024	3.042LysTyr: 3.042 ± 0.067	0.0LysXaa: 0.0 ± 0.0
Leu	7.624LeuAla: 7.624 ± 0.109	0.588LeuCys: 0.588 ± 0.028	6.13LeuAsp: 6.13 ± 0.088	8.57LeuGlu: 8.57 ± 0.122	3.816LeuPhe: 3.816 ± 0.074	7.012LeuGly: 7.012 ± 0.116	1.307LeuHis: 1.307 ± 0.039	8.93LeuIle: 8.93 ± 0.133	8.331LeuLys: 8.331 ± 0.109	10.013LeuLeu: 10.013 ± 0.139	2.079LeuMet: 2.079 ± 0.05	5.475LeuAsn: 5.475 ± 0.091	3.235LeuPro: 3.235 ± 0.067	2.605LeuGln: 2.605 ± 0.046	3.671LeuArg: 3.671 ± 0.061	6.662LeuSer: 6.662 ± 0.103	5.033LeuThr: 5.033 ± 0.08	6.683LeuVal: 6.683 ± 0.099	0.638LeuTrp: 0.638 ± 0.031	3.154LeuTyr: 3.154 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	1.701MetAla: 1.701 ± 0.042	0.102MetCys: 0.102 ± 0.01	1.146MetAsp: 1.146 ± 0.036	1.636MetGlu: 1.636 ± 0.045	0.67MetPhe: 0.67 ± 0.03	1.561MetGly: 1.561 ± 0.043	0.248MetHis: 0.248 ± 0.016	2.069MetIle: 2.069 ± 0.045	2.127MetLys: 2.127 ± 0.045	2.09MetLeu: 2.09 ± 0.048	0.625MetMet: 0.625 ± 0.033	1.236MetAsn: 1.236 ± 0.04	0.756MetPro: 0.756 ± 0.028	0.569MetGln: 0.569 ± 0.024	0.887MetArg: 0.887 ± 0.03	1.409MetSer: 1.409 ± 0.038	1.153MetThr: 1.153 ± 0.038	1.678MetVal: 1.678 ± 0.045	0.118MetTrp: 0.118 ± 0.012	0.539MetTyr: 0.539 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.224AsnAla: 2.224 ± 0.051	0.411AsnCys: 0.411 ± 0.023	2.51AsnAsp: 2.51 ± 0.051	2.933AsnGlu: 2.933 ± 0.06	2.452AsnPhe: 2.452 ± 0.057	2.835AsnGly: 2.835 ± 0.066	0.906AsnHis: 0.906 ± 0.034	4.99AsnIle: 4.99 ± 0.075	4.707AsnLys: 4.707 ± 0.074	6.502AsnLeu: 6.502 ± 0.107	1.202AsnMet: 1.202 ± 0.037	3.765AsnAsn: 3.765 ± 0.079	2.324AsnPro: 2.324 ± 0.051	2.548AsnGln: 2.548 ± 0.061	2.26AsnArg: 2.26 ± 0.057	3.244AsnSer: 3.244 ± 0.063	2.24AsnThr: 2.24 ± 0.06	2.467AsnVal: 2.467 ± 0.055	0.558AsnTrp: 0.558 ± 0.028	2.607AsnTyr: 2.607 ± 0.065	0.0AsnXaa: 0.0 ± 0.0
Pro	1.868ProAla: 1.868 ± 0.047	0.228ProCys: 0.228 ± 0.017	1.67ProAsp: 1.67 ± 0.046	2.664ProGlu: 2.664 ± 0.059	1.324ProPhe: 1.324 ± 0.041	1.809ProGly: 1.809 ± 0.047	0.539ProHis: 0.539 ± 0.023	2.728ProIle: 2.728 ± 0.056	2.128ProLys: 2.128 ± 0.049	2.902ProLeu: 2.902 ± 0.064	0.586ProMet: 0.586 ± 0.023	1.51ProAsn: 1.51 ± 0.043	0.777ProPro: 0.777 ± 0.032	1.086ProGln: 1.086 ± 0.036	0.996ProArg: 0.996 ± 0.033	1.482ProSer: 1.482 ± 0.042	1.67ProThr: 1.67 ± 0.048	2.127ProVal: 2.127 ± 0.045	0.312ProTrp: 0.312 ± 0.018	1.255ProTyr: 1.255 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.18GlnAla: 2.18 ± 0.049	0.145GlnCys: 0.145 ± 0.013	1.717GlnAsp: 1.717 ± 0.043	3.105GlnGlu: 3.105 ± 0.064	1.072GlnPhe: 1.072 ± 0.036	2.138GlnGly: 2.138 ± 0.043	0.376GlnHis: 0.376 ± 0.022	2.597GlnIle: 2.597 ± 0.061	2.345GlnLys: 2.345 ± 0.049	3.125GlnLeu: 3.125 ± 0.064	0.663GlnMet: 0.663 ± 0.029	1.413GlnAsn: 1.413 ± 0.038	0.793GlnPro: 0.793 ± 0.03	1.197GlnGln: 1.197 ± 0.046	1.32GlnArg: 1.32 ± 0.037	1.561GlnSer: 1.561 ± 0.046	1.185GlnThr: 1.185 ± 0.034	2.275GlnVal: 2.275 ± 0.05	0.245GlnTrp: 0.245 ± 0.018	0.911GlnTyr: 0.911 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.221ArgAla: 2.221 ± 0.052	0.301ArgCys: 0.301 ± 0.018	2.403ArgAsp: 2.403 ± 0.05	3.594ArgGlu: 3.594 ± 0.066	1.58ArgPhe: 1.58 ± 0.046	2.505ArgGly: 2.505 ± 0.044	0.571ArgHis: 0.571 ± 0.028	3.548ArgIle: 3.548 ± 0.052	2.942ArgLys: 2.942 ± 0.055	4.219ArgLeu: 4.219 ± 0.081	0.991ArgMet: 0.991 ± 0.036	1.807ArgAsn: 1.807 ± 0.053	1.148ArgPro: 1.148 ± 0.034	1.075ArgGln: 1.075 ± 0.035	1.926ArgArg: 1.926 ± 0.046	1.863ArgSer: 1.863 ± 0.039	1.689ArgThr: 1.689 ± 0.043	3.018ArgVal: 3.018 ± 0.057	0.321ArgTrp: 0.321 ± 0.016	1.641ArgTyr: 1.641 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.158SerAla: 3.158 ± 0.058	0.447SerCys: 0.447 ± 0.024	3.015SerAsp: 3.015 ± 0.062	3.718SerGlu: 3.718 ± 0.072	2.793SerPhe: 2.793 ± 0.056	3.89SerGly: 3.89 ± 0.069	0.882SerHis: 0.882 ± 0.032	5.209SerIle: 5.209 ± 0.094	4.922SerLys: 4.922 ± 0.09	6.328SerLeu: 6.328 ± 0.097	1.205SerMet: 1.205 ± 0.036	3.28SerAsn: 3.28 ± 0.066	1.737SerPro: 1.737 ± 0.045	2.122SerGln: 2.122 ± 0.05	2.25SerArg: 2.25 ± 0.051	3.851SerSer: 3.851 ± 0.073	2.837SerThr: 2.837 ± 0.063	3.071SerVal: 3.071 ± 0.067	0.559SerTrp: 0.559 ± 0.027	2.444SerTyr: 2.444 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.146ThrAla: 3.146 ± 0.058	0.304ThrCys: 0.304 ± 0.02	2.586ThrAsp: 2.586 ± 0.049	3.306ThrGlu: 3.306 ± 0.065	1.848ThrPhe: 1.848 ± 0.046	3.873ThrGly: 3.873 ± 0.072	0.709ThrHis: 0.709 ± 0.032	4.316ThrIle: 4.316 ± 0.073	3.485ThrLys: 3.485 ± 0.068	4.771ThrLeu: 4.771 ± 0.083	0.93ThrMet: 0.93 ± 0.033	2.281ThrAsn: 2.281 ± 0.054	1.783ThrPro: 1.783 ± 0.047	1.118ThrGln: 1.118 ± 0.042	1.579ThrArg: 1.579 ± 0.044	2.572ThrSer: 2.572 ± 0.054	2.707ThrThr: 2.707 ± 0.071	3.285ThrVal: 3.285 ± 0.064	0.292ThrTrp: 0.292 ± 0.021	1.713ThrTyr: 1.713 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.751ValAla: 4.751 ± 0.086	0.473ValCys: 0.473 ± 0.023	4.448ValAsp: 4.448 ± 0.085	5.617ValGlu: 5.617 ± 0.094	2.344ValPhe: 2.344 ± 0.057	4.774ValGly: 4.774 ± 0.086	0.855ValHis: 0.855 ± 0.029	6.251ValIle: 6.251 ± 0.083	5.569ValLys: 5.569 ± 0.069	5.958ValLeu: 5.958 ± 0.096	1.479ValMet: 1.479 ± 0.044	3.372ValAsn: 3.372 ± 0.067	2.0ValPro: 2.0 ± 0.044	1.483ValGln: 1.483 ± 0.047	2.398ValArg: 2.398 ± 0.054	3.965ValSer: 3.965 ± 0.07	3.393ValThr: 3.393 ± 0.064	4.875ValVal: 4.875 ± 0.082	0.468ValTrp: 0.468 ± 0.027	2.069ValTyr: 2.069 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.425TrpAla: 0.425 ± 0.024	0.064TrpCys: 0.064 ± 0.01	0.5TrpAsp: 0.5 ± 0.031	0.63TrpGlu: 0.63 ± 0.028	0.313TrpPhe: 0.313 ± 0.02	0.594TrpGly: 0.594 ± 0.027	0.115TrpHis: 0.115 ± 0.011	0.59TrpIle: 0.59 ± 0.025	0.533TrpLys: 0.533 ± 0.024	0.819TrpLeu: 0.819 ± 0.036	0.188TrpMet: 0.188 ± 0.016	0.479TrpAsn: 0.479 ± 0.024	0.184TrpPro: 0.184 ± 0.018	0.322TrpGln: 0.322 ± 0.021	0.311TrpArg: 0.311 ± 0.018	0.447TrpSer: 0.447 ± 0.024	0.375TrpThr: 0.375 ± 0.024	0.436TrpVal: 0.436 ± 0.02	0.118TrpTrp: 0.118 ± 0.012	0.3TrpTyr: 0.3 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.697TyrAla: 1.697 ± 0.045	0.354TyrCys: 0.354 ± 0.019	2.29TyrAsp: 2.29 ± 0.068	2.262TyrGlu: 2.262 ± 0.054	1.798TyrPhe: 1.798 ± 0.041	2.522TyrGly: 2.522 ± 0.057	0.787TyrHis: 0.787 ± 0.029	3.14TyrIle: 3.14 ± 0.062	2.86TyrLys: 2.86 ± 0.067	4.283TyrLeu: 4.283 ± 0.079	0.678TyrMet: 0.678 ± 0.03	2.565TyrAsn: 2.565 ± 0.063	1.344TyrPro: 1.344 ± 0.039	2.112TyrGln: 2.112 ± 0.049	1.689TyrArg: 1.689 ± 0.048	2.422TyrSer: 2.422 ± 0.055	1.78TyrThr: 1.78 ± 0.049	1.691TyrVal: 1.691 ± 0.045	0.39TyrTrp: 0.39 ± 0.026	1.883TyrTyr: 1.883 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.002	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3070 proteins (907535 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---