Wenzhou tombus-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH00|A0A1L3KH00_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 12 OX=1923665 PE=4 SV=1
MM1 pKa = 7.98EE2 pKa = 5.65DD3 pKa = 3.18QSSDD7 pKa = 3.51DD8 pKa = 3.67FEE10 pKa = 6.03SIVVSNSEE18 pKa = 3.74SSVRR22 pKa = 11.84IGSTEE27 pKa = 3.62PRR29 pKa = 11.84RR30 pKa = 11.84CGIDD34 pKa = 3.14PGDD37 pKa = 4.87SISQRR42 pKa = 11.84DD43 pKa = 3.67PGEE46 pKa = 4.18SSSEE50 pKa = 4.06EE51 pKa = 3.91EE52 pKa = 4.28EE53 pKa = 4.27EE54 pKa = 4.14EE55 pKa = 5.17QFQEE59 pKa = 4.42DD60 pKa = 4.63CGSSTSSCGSSSTSHH75 pKa = 6.57SSSQRR80 pKa = 11.84GSNSGGTVGGRR91 pKa = 11.84RR92 pKa = 11.84EE93 pKa = 4.41SGLLMAPGDD102 pKa = 3.56AGGEE106 pKa = 4.05VRR108 pKa = 11.84EE109 pKa = 4.46SVNQLEE115 pKa = 4.37EE116 pKa = 3.99HH117 pKa = 6.89DD118 pKa = 4.82CPFKK122 pKa = 11.02HH123 pKa = 6.55EE124 pKa = 4.25RR125 pKa = 11.84TTLFNPNYY133 pKa = 9.55NCCDD137 pKa = 3.16LGAWVRR143 pKa = 11.84PSVVEE148 pKa = 3.8YY149 pKa = 10.57DD150 pKa = 3.85KK151 pKa = 11.27PWFLFWSKK159 pKa = 10.65RR160 pKa = 11.84RR161 pKa = 11.84DD162 pKa = 3.58LVFSPVLVDD171 pKa = 3.33YY172 pKa = 8.86LKK174 pKa = 10.94PMAMLRR180 pKa = 11.84EE181 pKa = 4.04RR182 pKa = 11.84DD183 pKa = 3.62NALGLSLLTAARR195 pKa = 11.84NKK197 pKa = 9.47TISALGIHH205 pKa = 6.35PTWITPIFPGSISMAMQVGPMEE227 pKa = 4.34KK228 pKa = 9.42RR229 pKa = 11.84AKK231 pKa = 9.81EE232 pKa = 3.87LQKK235 pKa = 10.79PLIKK239 pKa = 10.59GKK241 pKa = 10.74LPGNWTLRR249 pKa = 11.84STHH252 pKa = 6.42KK253 pKa = 10.29PLEE256 pKa = 4.09RR257 pKa = 11.84LVEE260 pKa = 4.18NNSRR264 pKa = 11.84LGMSRR269 pKa = 11.84VRR271 pKa = 11.84SNQFVRR277 pKa = 11.84KK278 pKa = 8.77WIFGQSPEE286 pKa = 4.33LPRR289 pKa = 11.84IGG291 pKa = 3.91

Molecular weight: 32.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH00|A0A1L3KH00_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 12 OX=1923665 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84AQKK5 pKa = 10.18SANAVGGAVRR15 pKa = 11.84TPIKK19 pKa = 10.21GSKK22 pKa = 9.06PRR24 pKa = 11.84TSAEE28 pKa = 3.92VQKK31 pKa = 9.59TCRR34 pKa = 11.84QCKK37 pKa = 9.88APTRR41 pKa = 11.84LVKK44 pKa = 10.8GFVVCQQDD52 pKa = 3.46PNHH55 pKa = 6.05NFKK58 pKa = 10.23PVKK61 pKa = 9.49IVCGMCGAGRR71 pKa = 11.84SDD73 pKa = 3.52LVIYY77 pKa = 9.11PGKK80 pKa = 10.05VWCTKK85 pKa = 10.22CAATTIDD92 pKa = 3.46KK93 pKa = 9.94QAKK96 pKa = 8.38VEE98 pKa = 4.18KK99 pKa = 10.34KK100 pKa = 9.19VVNNKK105 pKa = 8.27VVQPKK110 pKa = 10.06AGKK113 pKa = 9.35PKK115 pKa = 9.04VKK117 pKa = 9.98SVKK120 pKa = 9.93SVPGNSPQKK129 pKa = 10.6KK130 pKa = 9.51KK131 pKa = 10.82IPFQPIGVNSRR142 pKa = 11.84GLKK145 pKa = 10.52SGDD148 pKa = 3.45LKK150 pKa = 11.02WFGKK154 pKa = 8.52TKK156 pKa = 7.57QTKK159 pKa = 4.59TTKK162 pKa = 10.34PKK164 pKa = 10.18FAWIKK169 pKa = 9.03KK170 pKa = 7.1TVGNVKK176 pKa = 10.22GLEE179 pKa = 3.82SHH181 pKa = 7.24IFYY184 pKa = 10.29TNKK187 pKa = 9.12TMQGAQLAALRR198 pKa = 11.84EE199 pKa = 4.51SPCFSCGGKK208 pKa = 9.66FATGGGWSSIEE219 pKa = 4.17PPSSSGKK226 pKa = 9.26ISKK229 pKa = 10.61GSIITMACPRR239 pKa = 11.84NTCLSEE245 pKa = 4.16YY246 pKa = 10.04NVVVVVEE253 pKa = 4.37KK254 pKa = 10.63KK255 pKa = 10.26KK256 pKa = 11.05KK257 pKa = 8.63EE258 pKa = 4.2FKK260 pKa = 9.94TLPLTGKK267 pKa = 9.5GKK269 pKa = 10.66KK270 pKa = 9.25ILTPQPPLAPEE281 pKa = 3.96TRR283 pKa = 11.84PVDD286 pKa = 3.9LSEE289 pKa = 4.95DD290 pKa = 3.54QVKK293 pKa = 8.03TVFDD297 pKa = 4.27EE298 pKa = 4.38EE299 pKa = 4.68SSRR302 pKa = 11.84HH303 pKa = 4.64NLRR306 pKa = 11.84EE307 pKa = 4.13KK308 pKa = 10.37IVSAFVAANEE318 pKa = 4.1DD319 pKa = 3.21FRR321 pKa = 11.84LADD324 pKa = 3.27SGARR328 pKa = 11.84QTLVNNARR336 pKa = 11.84RR337 pKa = 11.84QIKK340 pKa = 10.01LLEE343 pKa = 4.09RR344 pKa = 11.84QMPQRR349 pKa = 11.84LNLRR353 pKa = 11.84QKK355 pKa = 8.12TGPVRR360 pKa = 11.84TVPLRR365 pKa = 11.84WKK367 pKa = 9.96INRR370 pKa = 11.84RR371 pKa = 11.84TILNPLWSVTLSLLSEE387 pKa = 4.31SDD389 pKa = 3.26QLNPEE394 pKa = 4.13DD395 pKa = 4.13VEE397 pKa = 5.12LIQEE401 pKa = 4.29TAYY404 pKa = 10.68HH405 pKa = 5.8SVILEE410 pKa = 3.9SLLPKK415 pKa = 10.15KK416 pKa = 10.22KK417 pKa = 10.26KK418 pKa = 10.25KK419 pKa = 10.4NNSKK423 pKa = 8.5KK424 pKa = 8.06TAEE427 pKa = 4.25AVHH430 pKa = 5.55QVAEE434 pKa = 4.31AAPPPTPHH442 pKa = 7.18PSEE445 pKa = 3.95EE446 pKa = 4.2VTAEE450 pKa = 4.11EE451 pKa = 4.64LWGDD455 pKa = 3.87DD456 pKa = 4.43ASPDD460 pKa = 3.9SSWLQEE466 pKa = 4.27TQEE469 pKa = 4.03EE470 pKa = 5.11RR471 pKa = 11.84YY472 pKa = 10.14EE473 pKa = 4.13NQQ475 pKa = 2.85

Molecular weight: 52.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1666

291

475

416.5

46.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.122 ± 0.816

2.041 ± 0.435

4.502 ± 0.712

5.762 ± 0.949

3.902 ± 0.723

6.963 ± 0.508

2.041 ± 0.354

5.282 ± 0.734

6.543 ± 1.797

7.263 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.638

4.802 ± 0.452

6.783 ± 0.767

3.962 ± 0.442

6.783 ± 0.601

8.103 ± 1.392

5.522 ± 0.479

6.483 ± 0.629

1.981 ± 0.145

2.701 ± 0.834
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski