Hubei narna-like virus 9
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIN5|A0A1L3KIN5_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 9 OX=1922962 PE=4 SV=1
MM1 pKa = 7.3 PQAPQNNNKK10 pKa = 9.75 QNTKK14 pKa = 9.97 HH15 pKa = 6.63 SNAAMQKK22 pKa = 9.97 INEE25 pKa = 4.07 QNKK28 pKa = 8.62 NQNKK32 pKa = 7.84 SQKK35 pKa = 9.69 KK36 pKa = 7.67 VNHH39 pKa = 5.83 RR40 pKa = 11.84 RR41 pKa = 11.84 GYY43 pKa = 6.9 QQRR46 pKa = 11.84 PTLNQSQMFYY56 pKa = 11.03 AALTCPFDD64 pKa = 4.48 PNVLGVQVPDD74 pKa = 4.04 PFPYY78 pKa = 8.33 PTQVYY83 pKa = 9.06 HH84 pKa = 5.16 VHH86 pKa = 4.72 QTTVIGNAANNSGSGAIAFLPNPVLSLIDD115 pKa = 3.2 ITQANVGNITGSCIKK130 pKa = 9.43 ATPFTRR136 pKa = 11.84 YY137 pKa = 10.15 GPLTPTNVSNAILGAITPTALSDD160 pKa = 3.3 VFADD164 pKa = 3.53 YY165 pKa = 10.79 RR166 pKa = 11.84 VVSWGIKK173 pKa = 9.34 ISNLMPEE180 pKa = 4.71 LVATGRR186 pKa = 11.84 VIIAQIPLGDD196 pKa = 4.8 TIPSYY201 pKa = 11.29 PNLASAIQPVTLEE214 pKa = 4.48 SIFGIDD220 pKa = 3.5 PVFLGSSNILEE231 pKa = 4.76 LPTGFQLTAQDD242 pKa = 4.33 FLHH245 pKa = 7.28 GDD247 pKa = 4.11 LEE249 pKa = 4.76 CGGMYY254 pKa = 10.15 SAADD258 pKa = 3.47 FWDD261 pKa = 4.46 FKK263 pKa = 7.21 TTRR266 pKa = 11.84 DD267 pKa = 3.28 IGKK270 pKa = 9.25 LNVGGSPGTIFTGDD284 pKa = 3.53 DD285 pKa = 3.55 VAVTTSGLNYY295 pKa = 10.42 GIGYY299 pKa = 10.14 KK300 pKa = 10.8 DD301 pKa = 3.26 MTRR304 pKa = 11.84 CRR306 pKa = 11.84 GGSAIVIYY314 pKa = 10.07 FEE316 pKa = 5.18 GMPSNQIEE324 pKa = 4.24 NFFQVEE330 pKa = 4.54 TIYY333 pKa = 10.9 HH334 pKa = 6.46 LEE336 pKa = 4.0 GTPNFSSISNNALISSTARR355 pKa = 11.84 KK356 pKa = 6.98 TAVGTTQNVEE366 pKa = 3.79 QVMAKK371 pKa = 9.91 ASKK374 pKa = 10.17 VEE376 pKa = 4.93 NVFTWIDD383 pKa = 3.25 RR384 pKa = 11.84 GADD387 pKa = 3.42 FLNKK391 pKa = 10.0 NKK393 pKa = 9.24 STIMKK398 pKa = 9.87 VGAAAMAFLL407 pKa = 4.85
Molecular weight: 43.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.756
IPC2_protein 6.912
IPC_protein 7.088
Toseland 6.942
ProMoST 7.497
Dawson 7.702
Bjellqvist 7.658
Wikipedia 7.644
Rodwell 7.688
Grimsley 7.015
Solomon 7.819
Lehninger 7.834
Nozaki 7.922
DTASelect 7.819
Thurlkill 7.863
EMBOSS 7.966
Sillero 8.097
Patrickios 4.164
IPC_peptide 7.805
IPC2_peptide 7.176
IPC2.peptide.svr19 7.342
Protein with the highest isoelectric point:
>tr|A0A1L3KIN5|A0A1L3KIN5_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 9 OX=1922962 PE=4 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 NHH4 pKa = 6.75 LSFRR8 pKa = 11.84 EE9 pKa = 4.14 VCDD12 pKa = 3.52 GCLGSLTRR20 pKa = 11.84 GVPGTLKK27 pKa = 10.5 NSKK30 pKa = 9.52 VPRR33 pKa = 11.84 KK34 pKa = 9.71 RR35 pKa = 11.84 YY36 pKa = 9.52 FKK38 pKa = 10.66 IPGVRR43 pKa = 11.84 TWLKK47 pKa = 9.71 TSGGNYY53 pKa = 7.29 ITIFSTKK60 pKa = 10.06 NKK62 pKa = 9.9 IISKK66 pKa = 9.54 LLKK69 pKa = 9.95 EE70 pKa = 4.09 AMKK73 pKa = 10.89 VLTEE77 pKa = 3.72 SHH79 pKa = 6.17 GRR81 pKa = 11.84 STTDD85 pKa = 3.88 FSTFEE90 pKa = 3.4 NWYY93 pKa = 7.8 LHH95 pKa = 4.64 EE96 pKa = 4.66 VIRR99 pKa = 11.84 IFKK102 pKa = 8.86 ITDD105 pKa = 3.14 KK106 pKa = 10.76 TIEE109 pKa = 4.03 PEE111 pKa = 3.9 KK112 pKa = 10.61 LFRR115 pKa = 11.84 EE116 pKa = 4.4 ATDD119 pKa = 3.68 GFFEE123 pKa = 4.76 KK124 pKa = 10.77 ASFDD128 pKa = 4.99 SNLEE132 pKa = 3.98 WFWFNDD138 pKa = 3.15 SYY140 pKa = 11.31 LAEE143 pKa = 4.09 ILQFVSAEE151 pKa = 3.75 IHH153 pKa = 6.44 ILTDD157 pKa = 3.69 VIPDD161 pKa = 3.12 FHH163 pKa = 8.12 KK164 pKa = 10.53 RR165 pKa = 11.84 FSFEE169 pKa = 4.18 EE170 pKa = 3.93 QSKK173 pKa = 10.98 LYY175 pKa = 10.88 LNTLKK180 pKa = 10.38 IPRR183 pKa = 11.84 DD184 pKa = 3.59 SFIKK188 pKa = 10.29 HH189 pKa = 5.66 IKK191 pKa = 9.56 FHH193 pKa = 5.88 TACPMASILDD203 pKa = 3.49 QDD205 pKa = 4.92 LPAKK209 pKa = 10.34 PSDD212 pKa = 3.71 FTGHH216 pKa = 5.09 YY217 pKa = 9.49 LIWTGSVKK225 pKa = 10.35 RR226 pKa = 11.84 YY227 pKa = 9.61 LKK229 pKa = 10.78 NILNRR234 pKa = 11.84 RR235 pKa = 11.84 NFNSKK240 pKa = 8.5 DD241 pKa = 3.29 TPLCLKK247 pKa = 10.23 IGMGFLQGIKK257 pKa = 9.77 RR258 pKa = 11.84 GCATVPSSFLYY269 pKa = 10.74 SEE271 pKa = 4.91 VISHH275 pKa = 5.91 IHH277 pKa = 6.86 AMTTPPEE284 pKa = 4.13 LVPGWEE290 pKa = 4.24 YY291 pKa = 11.16 TDD293 pKa = 4.37 GFATKK298 pKa = 10.3 YY299 pKa = 10.31 SDD301 pKa = 5.86 PILQTFGATCEE312 pKa = 4.22 HH313 pKa = 6.64 VLKK316 pKa = 9.23 QTNKK320 pKa = 8.97 VYY322 pKa = 10.59 VPKK325 pKa = 10.17 PYY327 pKa = 10.25 EE328 pKa = 4.1 PSHH331 pKa = 5.62 NASFEE336 pKa = 4.03 RR337 pKa = 11.84 SRR339 pKa = 11.84 GEE341 pKa = 3.64 PEE343 pKa = 3.63 FEE345 pKa = 3.56 IEE347 pKa = 4.24 KK348 pKa = 9.93 FVKK351 pKa = 9.96 GGAYY355 pKa = 9.09 MEE357 pKa = 4.66 VVHH360 pKa = 6.93 SLGLQKK366 pKa = 9.11 ITKK369 pKa = 9.22 EE370 pKa = 4.21 VILKK374 pKa = 10.56 GDD376 pKa = 3.32 VGFNISYY383 pKa = 8.46 EE384 pKa = 4.21 LPDD387 pKa = 3.52 MDD389 pKa = 5.55 EE390 pKa = 4.07 VAEE393 pKa = 4.19 LCRR396 pKa = 11.84 IKK398 pKa = 10.98 YY399 pKa = 8.67 PSRR402 pKa = 11.84 FKK404 pKa = 11.01 FVDD407 pKa = 3.72 DD408 pKa = 4.54 DD409 pKa = 4.5 PLFKK413 pKa = 10.57 EE414 pKa = 4.28 YY415 pKa = 10.52 FPEE418 pKa = 4.4 LFVSSPQLSRR428 pKa = 11.84 EE429 pKa = 3.96 LSDD432 pKa = 3.34 TQVIPLCEE440 pKa = 3.87 PLKK443 pKa = 10.7 IRR445 pKa = 11.84 VITKK449 pKa = 10.55 GEE451 pKa = 3.96 ALPAYY456 pKa = 9.46 LAKK459 pKa = 9.92 TLQKK463 pKa = 9.83 TMKK466 pKa = 10.03 SYY468 pKa = 11.12 INRR471 pKa = 11.84 FPSMILTTRR480 pKa = 11.84 PLKK483 pKa = 10.75 VDD485 pKa = 3.52 DD486 pKa = 5.81 FRR488 pKa = 11.84 DD489 pKa = 3.06 VWARR493 pKa = 11.84 EE494 pKa = 3.87 KK495 pKa = 10.83 AIEE498 pKa = 4.15 SQLGINLKK506 pKa = 10.21 FCEE509 pKa = 4.69 HH510 pKa = 6.39 VSGDD514 pKa = 3.66 YY515 pKa = 10.38 KK516 pKa = 11.24 AATDD520 pKa = 3.69 KK521 pKa = 11.61 LNINFTKK528 pKa = 10.88 LIFEE532 pKa = 4.54 RR533 pKa = 11.84 FMVVLNIPQADD544 pKa = 3.65 RR545 pKa = 11.84 DD546 pKa = 4.01 VYY548 pKa = 11.01 RR549 pKa = 11.84 SVLYY553 pKa = 8.17 EE554 pKa = 3.84 QRR556 pKa = 11.84 VHH558 pKa = 6.54 YY559 pKa = 9.82 PFAAKK564 pKa = 10.41 LCRR567 pKa = 11.84 DD568 pKa = 3.42 KK569 pKa = 11.22 KK570 pKa = 11.05 LNSLNCLHH578 pKa = 7.09 KK579 pKa = 11.16 ANIEE583 pKa = 3.98 AKK585 pKa = 10.12 KK586 pKa = 10.42 LYY588 pKa = 9.87 KK589 pKa = 10.24 LEE591 pKa = 3.92 YY592 pKa = 9.67 AKK594 pKa = 10.09 WVSEE598 pKa = 3.8 GSNFLTEE605 pKa = 4.28 PVLPPLEE612 pKa = 4.15 FAVEE616 pKa = 4.06 QKK618 pKa = 10.62 NGQLMGSILSFPILCLANLICYY640 pKa = 9.64 KK641 pKa = 10.44 CALDD645 pKa = 3.95 EE646 pKa = 5.19 YY647 pKa = 11.0 INIDD651 pKa = 3.06 NKK653 pKa = 10.54 LRR655 pKa = 11.84 SKK657 pKa = 10.97 KK658 pKa = 9.86 FVNVFDD664 pKa = 5.25 LPVLINGDD672 pKa = 3.92 DD673 pKa = 3.2 IYY675 pKa = 11.26 FRR677 pKa = 11.84 SNPVFYY683 pKa = 9.96 EE684 pKa = 3.37 IWKK687 pKa = 9.85 KK688 pKa = 10.87 YY689 pKa = 9.88 IGIAGFVLSIGKK701 pKa = 9.91 NYY703 pKa = 7.34 VHH705 pKa = 7.3 KK706 pKa = 10.85 SIFTINSQCFRR717 pKa = 11.84 YY718 pKa = 9.99 NDD720 pKa = 3.63 EE721 pKa = 4.06 QDD723 pKa = 4.05 SIDD726 pKa = 4.17 EE727 pKa = 3.98 ITYY730 pKa = 10.82 LNVGLLIGQSKK741 pKa = 10.58 SGILGEE747 pKa = 5.47 KK748 pKa = 10.22 LPTWDD753 pKa = 4.73 LYY755 pKa = 11.91 NKK757 pKa = 8.07 VTVGSYY763 pKa = 11.12 NKK765 pKa = 9.56 VDD767 pKa = 2.99 SHH769 pKa = 7.21 NRR771 pKa = 11.84 FFYY774 pKa = 9.23 YY775 pKa = 10.38 HH776 pKa = 7.35 KK777 pKa = 10.84 DD778 pKa = 3.35 SIAQISKK785 pKa = 9.93 NGNYY789 pKa = 10.48 NLFLPKK795 pKa = 10.5 LLGGLGFIRR804 pKa = 11.84 PDD806 pKa = 3.18 PAIPVKK812 pKa = 9.75 ITRR815 pKa = 11.84 FQAQLGTYY823 pKa = 7.11 FHH825 pKa = 6.52 NRR827 pKa = 11.84 IIAAYY832 pKa = 6.51 KK833 pKa = 8.66 TPEE836 pKa = 4.24 KK837 pKa = 9.55 EE838 pKa = 3.86 LHH840 pKa = 5.91 MSQAHH845 pKa = 7.09 LIDD848 pKa = 3.6 EE849 pKa = 5.0 HH850 pKa = 8.82 SPDD853 pKa = 4.41 VYY855 pKa = 10.57 DD856 pKa = 4.33 TYY858 pKa = 11.43 LGDD861 pKa = 4.98 QIFQFIKK868 pKa = 10.22 KK869 pKa = 10.1 DD870 pKa = 3.49 EE871 pKa = 4.25 EE872 pKa = 4.34 VPEE875 pKa = 5.16 GYY877 pKa = 10.69 LPLNLSKK884 pKa = 10.24 RR885 pKa = 11.84 TEE887 pKa = 3.99 HH888 pKa = 7.02 LFVHH892 pKa = 6.88 DD893 pKa = 4.18 WGNFEE898 pKa = 4.53 PKK900 pKa = 10.55 LEE902 pKa = 3.95 FRR904 pKa = 11.84 SIDD907 pKa = 3.71 SQTLKK912 pKa = 10.68 HH913 pKa = 5.9 FRR915 pKa = 11.84 EE916 pKa = 4.49 SVKK919 pKa = 10.34 KK920 pKa = 10.0 YY921 pKa = 10.46 QGDD924 pKa = 3.77 KK925 pKa = 10.3 CWFGAEE931 pKa = 4.23 SAISGNYY938 pKa = 9.07 PYY940 pKa = 11.35 VLVRR944 pKa = 11.84 RR945 pKa = 11.84 VLDD948 pKa = 3.79 DD949 pKa = 3.61 VEE951 pKa = 4.82 TYY953 pKa = 10.41 QEE955 pKa = 3.86 NLRR958 pKa = 11.84 EE959 pKa = 4.0 VVYY962 pKa = 11.21 NKK964 pKa = 9.95 VLEE967 pKa = 4.46 NIKK970 pKa = 10.78 DD971 pKa = 3.69 YY972 pKa = 11.66 FEE974 pKa = 4.5 VKK976 pKa = 10.1 EE977 pKa = 4.12 KK978 pKa = 10.94 LKK980 pKa = 10.63 PLIFNGNPQVVTDD993 pKa = 3.46 VTTFF997 pKa = 3.03
Molecular weight: 115.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.296
IPC2_protein 7.732
IPC_protein 7.527
Toseland 7.351
ProMoST 8.126
Dawson 8.317
Bjellqvist 8.565
Wikipedia 8.273
Rodwell 8.375
Grimsley 7.424
Solomon 8.361
Lehninger 8.361
Nozaki 8.697
DTASelect 8.361
Thurlkill 8.434
EMBOSS 8.521
Sillero 8.697
Patrickios 4.304
IPC_peptide 8.361
IPC2_peptide 7.351
IPC2.peptide.svr19 7.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1404
407
997
702.0
79.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.271 ± 1.65
1.425 ± 0.236
5.057 ± 0.471
5.556 ± 1.527
6.125 ± 0.648
5.983 ± 1.137
2.279 ± 0.431
7.194 ± 0.095
7.55 ± 1.674
8.761 ± 1.27
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.567 ± 0.476
5.983 ± 1.269
5.342 ± 0.428
3.632 ± 1.08
3.846 ± 0.612
6.624 ± 0.137
6.054 ± 1.231
6.41 ± 0.251
1.068 ± 0.177
4.274 ± 0.709
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here