Hubei narna-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KIN5|A0A1L3KIN5_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 9 OX=1922962 PE=4 SV=1
MM1 pKa = 7.3PQAPQNNNKK10 pKa = 9.75QNTKK14 pKa = 9.97HH15 pKa = 6.63SNAAMQKK22 pKa = 9.97INEE25 pKa = 4.07QNKK28 pKa = 8.62NQNKK32 pKa = 7.84SQKK35 pKa = 9.69KK36 pKa = 7.67VNHH39 pKa = 5.83RR40 pKa = 11.84RR41 pKa = 11.84GYY43 pKa = 6.9QQRR46 pKa = 11.84PTLNQSQMFYY56 pKa = 11.03AALTCPFDD64 pKa = 4.48PNVLGVQVPDD74 pKa = 4.04PFPYY78 pKa = 8.33PTQVYY83 pKa = 9.06HH84 pKa = 5.16VHH86 pKa = 4.72QTTVIGNAANNSGSGAIAFLPNPVLSLIDD115 pKa = 3.2ITQANVGNITGSCIKK130 pKa = 9.43ATPFTRR136 pKa = 11.84YY137 pKa = 10.15GPLTPTNVSNAILGAITPTALSDD160 pKa = 3.3VFADD164 pKa = 3.53YY165 pKa = 10.79RR166 pKa = 11.84VVSWGIKK173 pKa = 9.34ISNLMPEE180 pKa = 4.71LVATGRR186 pKa = 11.84VIIAQIPLGDD196 pKa = 4.8TIPSYY201 pKa = 11.29PNLASAIQPVTLEE214 pKa = 4.48SIFGIDD220 pKa = 3.5PVFLGSSNILEE231 pKa = 4.76LPTGFQLTAQDD242 pKa = 4.33FLHH245 pKa = 7.28GDD247 pKa = 4.11LEE249 pKa = 4.76CGGMYY254 pKa = 10.15SAADD258 pKa = 3.47FWDD261 pKa = 4.46FKK263 pKa = 7.21TTRR266 pKa = 11.84DD267 pKa = 3.28IGKK270 pKa = 9.25LNVGGSPGTIFTGDD284 pKa = 3.53DD285 pKa = 3.55VAVTTSGLNYY295 pKa = 10.42GIGYY299 pKa = 10.14KK300 pKa = 10.8DD301 pKa = 3.26MTRR304 pKa = 11.84CRR306 pKa = 11.84GGSAIVIYY314 pKa = 10.07FEE316 pKa = 5.18GMPSNQIEE324 pKa = 4.24NFFQVEE330 pKa = 4.54TIYY333 pKa = 10.9HH334 pKa = 6.46LEE336 pKa = 4.0GTPNFSSISNNALISSTARR355 pKa = 11.84KK356 pKa = 6.98TAVGTTQNVEE366 pKa = 3.79QVMAKK371 pKa = 9.91ASKK374 pKa = 10.17VEE376 pKa = 4.93NVFTWIDD383 pKa = 3.25RR384 pKa = 11.84GADD387 pKa = 3.42FLNKK391 pKa = 10.0NKK393 pKa = 9.24STIMKK398 pKa = 9.87VGAAAMAFLL407 pKa = 4.85

Molecular weight:
43.97 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KIN5|A0A1L3KIN5_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 9 OX=1922962 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84NHH4 pKa = 6.75LSFRR8 pKa = 11.84EE9 pKa = 4.14VCDD12 pKa = 3.52GCLGSLTRR20 pKa = 11.84GVPGTLKK27 pKa = 10.5NSKK30 pKa = 9.52VPRR33 pKa = 11.84KK34 pKa = 9.71RR35 pKa = 11.84YY36 pKa = 9.52FKK38 pKa = 10.66IPGVRR43 pKa = 11.84TWLKK47 pKa = 9.71TSGGNYY53 pKa = 7.29ITIFSTKK60 pKa = 10.06NKK62 pKa = 9.9IISKK66 pKa = 9.54LLKK69 pKa = 9.95EE70 pKa = 4.09AMKK73 pKa = 10.89VLTEE77 pKa = 3.72SHH79 pKa = 6.17GRR81 pKa = 11.84STTDD85 pKa = 3.88FSTFEE90 pKa = 3.4NWYY93 pKa = 7.8LHH95 pKa = 4.64EE96 pKa = 4.66VIRR99 pKa = 11.84IFKK102 pKa = 8.86ITDD105 pKa = 3.14KK106 pKa = 10.76TIEE109 pKa = 4.03PEE111 pKa = 3.9KK112 pKa = 10.61LFRR115 pKa = 11.84EE116 pKa = 4.4ATDD119 pKa = 3.68GFFEE123 pKa = 4.76KK124 pKa = 10.77ASFDD128 pKa = 4.99SNLEE132 pKa = 3.98WFWFNDD138 pKa = 3.15SYY140 pKa = 11.31LAEE143 pKa = 4.09ILQFVSAEE151 pKa = 3.75IHH153 pKa = 6.44ILTDD157 pKa = 3.69VIPDD161 pKa = 3.12FHH163 pKa = 8.12KK164 pKa = 10.53RR165 pKa = 11.84FSFEE169 pKa = 4.18EE170 pKa = 3.93QSKK173 pKa = 10.98LYY175 pKa = 10.88LNTLKK180 pKa = 10.38IPRR183 pKa = 11.84DD184 pKa = 3.59SFIKK188 pKa = 10.29HH189 pKa = 5.66IKK191 pKa = 9.56FHH193 pKa = 5.88TACPMASILDD203 pKa = 3.49QDD205 pKa = 4.92LPAKK209 pKa = 10.34PSDD212 pKa = 3.71FTGHH216 pKa = 5.09YY217 pKa = 9.49LIWTGSVKK225 pKa = 10.35RR226 pKa = 11.84YY227 pKa = 9.61LKK229 pKa = 10.78NILNRR234 pKa = 11.84RR235 pKa = 11.84NFNSKK240 pKa = 8.5DD241 pKa = 3.29TPLCLKK247 pKa = 10.23IGMGFLQGIKK257 pKa = 9.77RR258 pKa = 11.84GCATVPSSFLYY269 pKa = 10.74SEE271 pKa = 4.91VISHH275 pKa = 5.91IHH277 pKa = 6.86AMTTPPEE284 pKa = 4.13LVPGWEE290 pKa = 4.24YY291 pKa = 11.16TDD293 pKa = 4.37GFATKK298 pKa = 10.3YY299 pKa = 10.31SDD301 pKa = 5.86PILQTFGATCEE312 pKa = 4.22HH313 pKa = 6.64VLKK316 pKa = 9.23QTNKK320 pKa = 8.97VYY322 pKa = 10.59VPKK325 pKa = 10.17PYY327 pKa = 10.25EE328 pKa = 4.1PSHH331 pKa = 5.62NASFEE336 pKa = 4.03RR337 pKa = 11.84SRR339 pKa = 11.84GEE341 pKa = 3.64PEE343 pKa = 3.63FEE345 pKa = 3.56IEE347 pKa = 4.24KK348 pKa = 9.93FVKK351 pKa = 9.96GGAYY355 pKa = 9.09MEE357 pKa = 4.66VVHH360 pKa = 6.93SLGLQKK366 pKa = 9.11ITKK369 pKa = 9.22EE370 pKa = 4.21VILKK374 pKa = 10.56GDD376 pKa = 3.32VGFNISYY383 pKa = 8.46EE384 pKa = 4.21LPDD387 pKa = 3.52MDD389 pKa = 5.55EE390 pKa = 4.07VAEE393 pKa = 4.19LCRR396 pKa = 11.84IKK398 pKa = 10.98YY399 pKa = 8.67PSRR402 pKa = 11.84FKK404 pKa = 11.01FVDD407 pKa = 3.72DD408 pKa = 4.54DD409 pKa = 4.5PLFKK413 pKa = 10.57EE414 pKa = 4.28YY415 pKa = 10.52FPEE418 pKa = 4.4LFVSSPQLSRR428 pKa = 11.84EE429 pKa = 3.96LSDD432 pKa = 3.34TQVIPLCEE440 pKa = 3.87PLKK443 pKa = 10.7IRR445 pKa = 11.84VITKK449 pKa = 10.55GEE451 pKa = 3.96ALPAYY456 pKa = 9.46LAKK459 pKa = 9.92TLQKK463 pKa = 9.83TMKK466 pKa = 10.03SYY468 pKa = 11.12INRR471 pKa = 11.84FPSMILTTRR480 pKa = 11.84PLKK483 pKa = 10.75VDD485 pKa = 3.52DD486 pKa = 5.81FRR488 pKa = 11.84DD489 pKa = 3.06VWARR493 pKa = 11.84EE494 pKa = 3.87KK495 pKa = 10.83AIEE498 pKa = 4.15SQLGINLKK506 pKa = 10.21FCEE509 pKa = 4.69HH510 pKa = 6.39VSGDD514 pKa = 3.66YY515 pKa = 10.38KK516 pKa = 11.24AATDD520 pKa = 3.69KK521 pKa = 11.61LNINFTKK528 pKa = 10.88LIFEE532 pKa = 4.54RR533 pKa = 11.84FMVVLNIPQADD544 pKa = 3.65RR545 pKa = 11.84DD546 pKa = 4.01VYY548 pKa = 11.01RR549 pKa = 11.84SVLYY553 pKa = 8.17EE554 pKa = 3.84QRR556 pKa = 11.84VHH558 pKa = 6.54YY559 pKa = 9.82PFAAKK564 pKa = 10.41LCRR567 pKa = 11.84DD568 pKa = 3.42KK569 pKa = 11.22KK570 pKa = 11.05LNSLNCLHH578 pKa = 7.09KK579 pKa = 11.16ANIEE583 pKa = 3.98AKK585 pKa = 10.12KK586 pKa = 10.42LYY588 pKa = 9.87KK589 pKa = 10.24LEE591 pKa = 3.92YY592 pKa = 9.67AKK594 pKa = 10.09WVSEE598 pKa = 3.8GSNFLTEE605 pKa = 4.28PVLPPLEE612 pKa = 4.15FAVEE616 pKa = 4.06QKK618 pKa = 10.62NGQLMGSILSFPILCLANLICYY640 pKa = 9.64KK641 pKa = 10.44CALDD645 pKa = 3.95EE646 pKa = 5.19YY647 pKa = 11.0INIDD651 pKa = 3.06NKK653 pKa = 10.54LRR655 pKa = 11.84SKK657 pKa = 10.97KK658 pKa = 9.86FVNVFDD664 pKa = 5.25LPVLINGDD672 pKa = 3.92DD673 pKa = 3.2IYY675 pKa = 11.26FRR677 pKa = 11.84SNPVFYY683 pKa = 9.96EE684 pKa = 3.37IWKK687 pKa = 9.85KK688 pKa = 10.87YY689 pKa = 9.88IGIAGFVLSIGKK701 pKa = 9.91NYY703 pKa = 7.34VHH705 pKa = 7.3KK706 pKa = 10.85SIFTINSQCFRR717 pKa = 11.84YY718 pKa = 9.99NDD720 pKa = 3.63EE721 pKa = 4.06QDD723 pKa = 4.05SIDD726 pKa = 4.17EE727 pKa = 3.98ITYY730 pKa = 10.82LNVGLLIGQSKK741 pKa = 10.58SGILGEE747 pKa = 5.47KK748 pKa = 10.22LPTWDD753 pKa = 4.73LYY755 pKa = 11.91NKK757 pKa = 8.07VTVGSYY763 pKa = 11.12NKK765 pKa = 9.56VDD767 pKa = 2.99SHH769 pKa = 7.21NRR771 pKa = 11.84FFYY774 pKa = 9.23YY775 pKa = 10.38HH776 pKa = 7.35KK777 pKa = 10.84DD778 pKa = 3.35SIAQISKK785 pKa = 9.93NGNYY789 pKa = 10.48NLFLPKK795 pKa = 10.5LLGGLGFIRR804 pKa = 11.84PDD806 pKa = 3.18PAIPVKK812 pKa = 9.75ITRR815 pKa = 11.84FQAQLGTYY823 pKa = 7.11FHH825 pKa = 6.52NRR827 pKa = 11.84IIAAYY832 pKa = 6.51KK833 pKa = 8.66TPEE836 pKa = 4.24KK837 pKa = 9.55EE838 pKa = 3.86LHH840 pKa = 5.91MSQAHH845 pKa = 7.09LIDD848 pKa = 3.6EE849 pKa = 5.0HH850 pKa = 8.82SPDD853 pKa = 4.41VYY855 pKa = 10.57DD856 pKa = 4.33TYY858 pKa = 11.43LGDD861 pKa = 4.98QIFQFIKK868 pKa = 10.22KK869 pKa = 10.1DD870 pKa = 3.49EE871 pKa = 4.25EE872 pKa = 4.34VPEE875 pKa = 5.16GYY877 pKa = 10.69LPLNLSKK884 pKa = 10.24RR885 pKa = 11.84TEE887 pKa = 3.99HH888 pKa = 7.02LFVHH892 pKa = 6.88DD893 pKa = 4.18WGNFEE898 pKa = 4.53PKK900 pKa = 10.55LEE902 pKa = 3.95FRR904 pKa = 11.84SIDD907 pKa = 3.71SQTLKK912 pKa = 10.68HH913 pKa = 5.9FRR915 pKa = 11.84EE916 pKa = 4.49SVKK919 pKa = 10.34KK920 pKa = 10.0YY921 pKa = 10.46QGDD924 pKa = 3.77KK925 pKa = 10.3CWFGAEE931 pKa = 4.23SAISGNYY938 pKa = 9.07PYY940 pKa = 11.35VLVRR944 pKa = 11.84RR945 pKa = 11.84VLDD948 pKa = 3.79DD949 pKa = 3.61VEE951 pKa = 4.82TYY953 pKa = 10.41QEE955 pKa = 3.86NLRR958 pKa = 11.84EE959 pKa = 4.0VVYY962 pKa = 11.21NKK964 pKa = 9.95VLEE967 pKa = 4.46NIKK970 pKa = 10.78DD971 pKa = 3.69YY972 pKa = 11.66FEE974 pKa = 4.5VKK976 pKa = 10.1EE977 pKa = 4.12KK978 pKa = 10.94LKK980 pKa = 10.63PLIFNGNPQVVTDD993 pKa = 3.46VTTFF997 pKa = 3.03

Molecular weight:
115.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1404

407

997

702.0

79.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.271 ± 1.65

1.425 ± 0.236

5.057 ± 0.471

5.556 ± 1.527

6.125 ± 0.648

5.983 ± 1.137

2.279 ± 0.431

7.194 ± 0.095

7.55 ± 1.674

8.761 ± 1.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.567 ± 0.476

5.983 ± 1.269

5.342 ± 0.428

3.632 ± 1.08

3.846 ± 0.612

6.624 ± 0.137

6.054 ± 1.231

6.41 ± 0.251

1.068 ± 0.177

4.274 ± 0.709

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski