Leptolyngbya sp. Heron Island J

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Leptolyngbya; unclassified Leptolyngbya

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U9VW03|U9VW03_9CYAN Alpha-amylase alpha-mannosidase OS=Leptolyngbya sp. Heron Island J OX=1385935 GN=N836_05715 PE=4 SV=1
MM1 pKa = 7.06TNLWKK6 pKa = 10.64AVMAGPLLLGAVFSSSNIAAEE27 pKa = 3.95AAEE30 pKa = 4.46TVSVDD35 pKa = 3.26ALSNSDD41 pKa = 4.5SIQLAQVTSVSEE53 pKa = 4.16LSDD56 pKa = 3.5VLPSDD61 pKa = 3.25WAYY64 pKa = 9.14TALQRR69 pKa = 11.84LVEE72 pKa = 4.32EE73 pKa = 4.49YY74 pKa = 11.08GCIEE78 pKa = 5.0GYY80 pKa = 9.42PDD82 pKa = 3.41RR83 pKa = 11.84SFRR86 pKa = 11.84GNRR89 pKa = 11.84AMTRR93 pKa = 11.84YY94 pKa = 9.34EE95 pKa = 3.88FAAGLNACLDD105 pKa = 3.87VVVQLIDD112 pKa = 4.27ASPDD116 pKa = 3.37DD117 pKa = 4.7LDD119 pKa = 3.99TIRR122 pKa = 11.84RR123 pKa = 11.84LQEE126 pKa = 3.36EE127 pKa = 4.63FAAEE131 pKa = 3.85LATIRR136 pKa = 11.84GRR138 pKa = 11.84VDD140 pKa = 2.9TLEE143 pKa = 5.27ADD145 pKa = 3.89VAEE148 pKa = 4.62LEE150 pKa = 4.39ANQFSTTTKK159 pKa = 10.81LKK161 pKa = 10.31GQLDD165 pKa = 3.64AHH167 pKa = 6.23LVVPFDD173 pKa = 4.0GPGDD177 pKa = 3.7LDD179 pKa = 3.83EE180 pKa = 5.01PTFQYY185 pKa = 8.87RR186 pKa = 11.84TRR188 pKa = 11.84LNLDD192 pKa = 3.23TSFTGEE198 pKa = 3.71DD199 pKa = 3.16RR200 pKa = 11.84LRR202 pKa = 11.84TRR204 pKa = 11.84LQFGDD209 pKa = 3.89ANNALSGAPGGFAAAGSGGFVDD231 pKa = 5.76DD232 pKa = 4.16VRR234 pKa = 11.84IDD236 pKa = 3.46DD237 pKa = 4.14VYY239 pKa = 11.06YY240 pKa = 10.62SFPIGSRR247 pKa = 11.84VSAIIAANSIVSDD260 pKa = 4.11DD261 pKa = 4.19FVTSTIVPFDD271 pKa = 3.97GPSVADD277 pKa = 3.23YY278 pKa = 9.9GGPMFYY284 pKa = 10.67DD285 pKa = 3.3QLGLGGQSFAAGFNIALTDD304 pKa = 4.23SLILDD309 pKa = 4.12AGYY312 pKa = 8.24ATSQGQDD319 pKa = 2.77ADD321 pKa = 3.58KK322 pKa = 11.51GIFDD326 pKa = 3.81EE327 pKa = 5.09YY328 pKa = 11.09EE329 pKa = 3.86YY330 pKa = 11.09LVQLNYY336 pKa = 10.87LSDD339 pKa = 3.89GLIDD343 pKa = 3.86AAVTYY348 pKa = 10.11MDD350 pKa = 4.84GEE352 pKa = 4.51SGDD355 pKa = 4.09GNGADD360 pKa = 4.73GIIAGLLSLDD370 pKa = 3.62FGGFQIGGYY379 pKa = 9.77YY380 pKa = 10.23ADD382 pKa = 3.78QDD384 pKa = 4.44GDD386 pKa = 4.02EE387 pKa = 4.18SWQAGVAVSNLLGTGNAAGVYY408 pKa = 9.33YY409 pKa = 10.94VEE411 pKa = 5.64DD412 pKa = 4.47FNDD415 pKa = 4.01DD416 pKa = 3.57EE417 pKa = 4.44TAEE420 pKa = 4.87AYY422 pKa = 10.87YY423 pKa = 11.17SMQINEE429 pKa = 4.41YY430 pKa = 7.26WTLTPAVIYY439 pKa = 9.81GQFDD443 pKa = 3.51ATGDD447 pKa = 3.84EE448 pKa = 4.26SWYY451 pKa = 10.32GAIRR455 pKa = 11.84SRR457 pKa = 11.84FKK459 pKa = 10.92FF460 pKa = 3.48

Molecular weight: 49.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U9W736|U9W736_9CYAN Uncharacterized protein OS=Leptolyngbya sp. Heron Island J OX=1385935 GN=N836_30615 PE=3 SV=1
MM1 pKa = 7.57ANKK4 pKa = 9.85IYY6 pKa = 9.76PLKK9 pKa = 10.42PSYY12 pKa = 7.69TTSPTISPPANVFNRR27 pKa = 11.84IISRR31 pKa = 11.84RR32 pKa = 11.84LKK34 pKa = 10.6AFCCSRR40 pKa = 11.84LSRR43 pKa = 11.84FVLLSLVLLITFLVSLALGSVAIPPSEE70 pKa = 4.09VMTVLLGGTATKK82 pKa = 9.71PAWQEE87 pKa = 3.55IILTFRR93 pKa = 11.84LPRR96 pKa = 11.84SLTALAAGSALAISGLQLQALFRR119 pKa = 11.84NALAGPSVLGINAGASLGAALVVLTGGGIGWWGQLSVVGAASGGAAIAMLLVLLMARR176 pKa = 11.84QVRR179 pKa = 11.84SSLALLIFGLMLGYY193 pKa = 8.81VTTALVTILLHH204 pKa = 6.85FSRR207 pKa = 11.84LEE209 pKa = 3.64QTLAYY214 pKa = 10.39LNWTFGSFNGVTWQQMPILLSVVTMGLVIAQLTCKK249 pKa = 10.05PLNLLLLGEE258 pKa = 4.37EE259 pKa = 4.33QAISLGLSVTVVRR272 pKa = 11.84CYY274 pKa = 10.97LIFSASLLAGSITAFCGPIAFLGVAVPHH302 pKa = 6.93LGRR305 pKa = 11.84SLFRR309 pKa = 11.84TQDD312 pKa = 2.91HH313 pKa = 7.18RR314 pKa = 11.84ILVPVVAFLGGWLALVADD332 pKa = 5.62SIAQVPGNQTVLPLNAVMALLGAPVLIWLMLKK364 pKa = 10.05QGRR367 pKa = 11.84QQII370 pKa = 3.83

Molecular weight: 39.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7108

0

7108

2230696

29

4624

313.8

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.963 ± 0.034

0.972 ± 0.011

5.496 ± 0.03

5.828 ± 0.029

3.808 ± 0.021

6.876 ± 0.038

2.063 ± 0.016

5.999 ± 0.022

3.669 ± 0.03

11.237 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.008 ± 0.015

3.729 ± 0.022

4.864 ± 0.022

5.518 ± 0.028

5.421 ± 0.022

6.374 ± 0.026

5.966 ± 0.026

6.79 ± 0.023

1.516 ± 0.017

2.902 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski