Chaetura pelagica (Chimney swift)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3162 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A093BQW3|A0A093BQW3_CHAPE Calpain-5 OS=Chaetura pelagica OX=8897 GN=M959_11815 PE=3 SV=1
SS1 pKa = 6.72 RR2 pKa = 11.84 LSWLEE7 pKa = 3.51 NSGVGFSLDD16 pKa = 3.46 YY17 pKa = 9.24 PTISLHH23 pKa = 6.26 AVSRR27 pKa = 11.84 DD28 pKa = 3.06 LAAYY32 pKa = 8.27 PCEE35 pKa = 3.94 HH36 pKa = 7.07 LYY38 pKa = 11.68 VMVNAKK44 pKa = 10.31 FEE46 pKa = 4.16 EE47 pKa = 4.74 EE48 pKa = 4.02 EE49 pKa = 4.28 TKK51 pKa = 10.49 EE52 pKa = 4.18 VPVEE56 pKa = 3.93 EE57 pKa = 5.0 GEE59 pKa = 4.9 EE60 pKa = 4.02 EE61 pKa = 5.3 DD62 pKa = 4.94 SDD64 pKa = 5.66 DD65 pKa = 3.95 DD66 pKa = 4.44 AEE68 pKa = 5.13 PIAEE72 pKa = 4.02 FRR74 pKa = 11.84 FVPSDD79 pKa = 3.36 KK80 pKa = 10.88 SALEE84 pKa = 3.9 AMFSAMCEE92 pKa = 4.27 CQALHH97 pKa = 7.41 PDD99 pKa = 4.21 PDD101 pKa = 5.77 DD102 pKa = 4.05 EE103 pKa = 7.61 DD104 pKa = 4.46 SDD106 pKa = 3.89 NDD108 pKa = 3.96 YY109 pKa = 11.58 EE110 pKa = 4.77 GDD112 pKa = 3.7 EE113 pKa = 4.54 YY114 pKa = 11.44 DD115 pKa = 4.51 VEE117 pKa = 4.31 AHH119 pKa = 6.63 EE120 pKa = 5.44 LGQGDD125 pKa = 3.72 IPTFYY130 pKa = 10.0 TYY132 pKa = 11.09 EE133 pKa = 4.17 EE134 pKa = 4.62 GLSHH138 pKa = 6.9 LTAEE142 pKa = 4.71 GQATLEE148 pKa = 4.11 RR149 pKa = 11.84 LEE151 pKa = 4.74 GMLAQSVSSQYY162 pKa = 11.66 NMAGVRR168 pKa = 11.84 TEE170 pKa = 3.7 DD171 pKa = 3.58 SIRR174 pKa = 11.84 EE175 pKa = 4.1 FEE177 pKa = 5.27 DD178 pKa = 3.49 GMEE181 pKa = 4.04 VDD183 pKa = 3.86 VAPVVAGQFEE193 pKa = 4.42 DD194 pKa = 5.26 AEE196 pKa = 4.32 VDD198 pKa = 3.59 HH199 pKa = 7.08
Molecular weight: 22.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.567
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.872
Patrickios 1.176
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A093BL38|A0A093BL38_CHAPE Complexin-1 (Fragment) OS=Chaetura pelagica OX=8897 GN=M959_05701 PE=3 SV=1
LL1 pKa = 7.12 GRR3 pKa = 11.84 GSLGRR8 pKa = 11.84 GSLGRR13 pKa = 11.84 GSLGRR18 pKa = 11.84 TSMGRR23 pKa = 11.84 RR24 pKa = 11.84 SLWRR28 pKa = 11.84 RR29 pKa = 11.84 SLGRR33 pKa = 11.84 GSLGRR38 pKa = 11.84 RR39 pKa = 11.84 SLGRR43 pKa = 11.84 RR44 pKa = 11.84 SLGRR48 pKa = 11.84 GSLGRR53 pKa = 11.84 TSMGG57 pKa = 3.08
Molecular weight: 6.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.647
Grimsley 13.188
Solomon 13.642
Lehninger 13.539
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.369
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.297
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3162
0
3162
1199214
34
4816
379.3
42.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.643 ± 0.037
2.294 ± 0.042
4.936 ± 0.029
7.113 ± 0.064
3.847 ± 0.038
6.22 ± 0.054
2.622 ± 0.026
4.634 ± 0.033
6.102 ± 0.06
9.995 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.203 ± 0.018
3.747 ± 0.031
5.61 ± 0.06
4.816 ± 0.044
5.401 ± 0.033
8.022 ± 0.063
5.298 ± 0.03
6.386 ± 0.043
1.217 ± 0.017
2.892 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here