Vibrio nereis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M0HLW4|A0A0M0HLW4_VIBNE Aerotolerance protein BatD OS=Vibrio nereis OX=693 GN=AKJ17_10815 PE=4 SV=1
MM1 pKa = 7.41SHH3 pKa = 6.51EE4 pKa = 4.59DD5 pKa = 3.39EE6 pKa = 4.49YY7 pKa = 11.8LSVEE11 pKa = 3.99QLIEE15 pKa = 3.92IQKK18 pKa = 10.62EE19 pKa = 3.95EE20 pKa = 4.09TRR22 pKa = 11.84DD23 pKa = 3.32IIQALLEE30 pKa = 5.14DD31 pKa = 4.8GSDD34 pKa = 3.52PDD36 pKa = 3.38SLYY39 pKa = 10.88DD40 pKa = 3.82IEE42 pKa = 4.6HH43 pKa = 6.83HH44 pKa = 7.2LFAEE48 pKa = 4.84DD49 pKa = 4.41FNTLEE54 pKa = 4.27KK55 pKa = 10.85AVVEE59 pKa = 4.0AFKK62 pKa = 10.62MGFEE66 pKa = 4.02VLEE69 pKa = 4.38AEE71 pKa = 4.32EE72 pKa = 4.84TEE74 pKa = 5.32DD75 pKa = 4.16EE76 pKa = 4.91DD77 pKa = 5.29GNKK80 pKa = 9.92LLCCDD85 pKa = 3.51ATMEE89 pKa = 4.38SALDD93 pKa = 3.6AEE95 pKa = 5.5AIDD98 pKa = 3.84AQVEE102 pKa = 4.49KK103 pKa = 10.53LVHH106 pKa = 5.94LAEE109 pKa = 5.18KK110 pKa = 10.82YY111 pKa = 10.77DD112 pKa = 3.78IIYY115 pKa = 10.15DD116 pKa = 3.64GWGTYY121 pKa = 9.39YY122 pKa = 10.49EE123 pKa = 5.16GEE125 pKa = 4.09DD126 pKa = 3.16ALYY129 pKa = 10.19PEE131 pKa = 5.05EE132 pKa = 4.98EE133 pKa = 4.65DD134 pKa = 5.46DD135 pKa = 4.98EE136 pKa = 4.56EE137 pKa = 4.35

Molecular weight: 15.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M0HSR8|A0A0M0HSR8_VIBNE ThrE domain-containing protein OS=Vibrio nereis OX=693 GN=AKJ17_02630 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84ATINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3660

0

3660

1178257

37

1614

321.9

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.673 ± 0.038

1.043 ± 0.013

5.433 ± 0.028

6.657 ± 0.039

4.09 ± 0.026

6.898 ± 0.037

2.232 ± 0.02

6.234 ± 0.031

5.356 ± 0.027

10.347 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.018

4.088 ± 0.022

3.911 ± 0.023

4.51 ± 0.031

4.487 ± 0.031

6.587 ± 0.035

5.249 ± 0.027

7.231 ± 0.031

1.249 ± 0.017

2.994 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski