Cuban alphasatellite 1

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; unclassified Begomovirus-associated alphasatellites

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6CRB1|S6CRB1_9VIRU Rep OS=Cuban alphasatellite 1 OX=1188819 GN=V1 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 9.94SRR5 pKa = 11.84NYY7 pKa = 10.4CFTLFSYY14 pKa = 9.58VLPLFNSLPDD24 pKa = 3.31WANYY28 pKa = 10.28LVFQEE33 pKa = 4.78EE34 pKa = 4.64EE35 pKa = 4.28SPSTGRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.55IQGYY47 pKa = 10.2VNLKK51 pKa = 9.96SPQSFSFLKK60 pKa = 10.5KK61 pKa = 9.76KK62 pKa = 10.78LGDD65 pKa = 3.86GVHH68 pKa = 6.76LEE70 pKa = 4.03QARR73 pKa = 11.84GSASCNRR80 pKa = 11.84DD81 pKa = 3.05YY82 pKa = 11.22CRR84 pKa = 11.84KK85 pKa = 7.55TDD87 pKa = 3.52SRR89 pKa = 11.84VSGPWEE95 pKa = 3.82FGILAEE101 pKa = 4.33QGSKK105 pKa = 9.95KK106 pKa = 10.06RR107 pKa = 11.84KK108 pKa = 7.07TMEE111 pKa = 4.01SFQEE115 pKa = 4.17DD116 pKa = 4.27PEE118 pKa = 4.18EE119 pKa = 4.99LRR121 pKa = 11.84LSDD124 pKa = 3.31PKK126 pKa = 10.91LYY128 pKa = 10.27RR129 pKa = 11.84RR130 pKa = 11.84CLATRR135 pKa = 11.84VNTEE139 pKa = 3.42FAGLVLPVLDD149 pKa = 5.35RR150 pKa = 11.84PWQLLVEE157 pKa = 4.47KK158 pKa = 10.54VLDD161 pKa = 4.18EE162 pKa = 4.9GPDD165 pKa = 3.38DD166 pKa = 3.88RR167 pKa = 11.84TIIWVYY173 pKa = 10.56GSQGNEE179 pKa = 4.34GKK181 pKa = 7.1TTWAKK186 pKa = 10.81SKK188 pKa = 10.64VQAGWFYY195 pKa = 11.16SRR197 pKa = 11.84GGKK200 pKa = 9.86GEE202 pKa = 4.16NIKK205 pKa = 10.8YY206 pKa = 10.46SYY208 pKa = 11.0AEE210 pKa = 4.1HH211 pKa = 6.89LGHH214 pKa = 7.21AVFDD218 pKa = 3.76IPRR221 pKa = 11.84QVEE224 pKa = 4.18DD225 pKa = 3.31VLQYY229 pKa = 10.56TVLEE233 pKa = 4.5EE234 pKa = 3.95IKK236 pKa = 10.78DD237 pKa = 3.69RR238 pKa = 11.84LIRR241 pKa = 11.84SSKK244 pKa = 10.56YY245 pKa = 10.02EE246 pKa = 4.51PIDD249 pKa = 3.92FNCSDD254 pKa = 3.5QVHH257 pKa = 5.25VVVLSNFLPQLDD269 pKa = 4.43SEE271 pKa = 4.5HH272 pKa = 7.42DD273 pKa = 3.61SRR275 pKa = 11.84GNLIKK280 pKa = 10.61KK281 pKa = 9.93QMLSRR286 pKa = 11.84DD287 pKa = 2.9RR288 pKa = 11.84VVIVNIAEE296 pKa = 4.15SLIVRR301 pKa = 11.84DD302 pKa = 3.66NEE304 pKa = 4.23TVSFHH309 pKa = 7.24EE310 pKa = 4.25YY311 pKa = 9.9MEE313 pKa = 4.37

Molecular weight: 36.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6CRB1|S6CRB1_9VIRU Rep OS=Cuban alphasatellite 1 OX=1188819 GN=V1 PE=4 SV=1
MM1 pKa = 7.41GFISNKK7 pKa = 10.1PGDD10 pKa = 4.05LHH12 pKa = 6.36PAIEE16 pKa = 4.88IIAEE20 pKa = 4.11RR21 pKa = 11.84LTLASLGHH29 pKa = 6.41GSSAYY34 pKa = 10.16LRR36 pKa = 11.84NRR38 pKa = 11.84GARR41 pKa = 11.84RR42 pKa = 11.84GRR44 pKa = 11.84RR45 pKa = 11.84WNHH48 pKa = 4.29FRR50 pKa = 11.84KK51 pKa = 7.52TQRR54 pKa = 11.84NYY56 pKa = 11.26DD57 pKa = 3.96CPTLNSIVAAWRR69 pKa = 11.84LGLIRR74 pKa = 11.84SSLVWYY80 pKa = 10.23SLYY83 pKa = 10.2LTDD86 pKa = 6.02HH87 pKa = 6.43GSCWLRR93 pKa = 11.84RR94 pKa = 11.84YY95 pKa = 7.29STRR98 pKa = 11.84AQMIEE103 pKa = 3.86LSYY106 pKa = 11.27GCMALRR112 pKa = 11.84VMKK115 pKa = 10.58GKK117 pKa = 10.66LPGQSLRR124 pKa = 11.84FKK126 pKa = 10.97LGGSTLEE133 pKa = 4.06EE134 pKa = 4.22EE135 pKa = 4.48KK136 pKa = 10.88EE137 pKa = 4.39KK138 pKa = 10.57TSSTHH143 pKa = 5.1TLNTSAMLCSTSHH156 pKa = 6.5AKK158 pKa = 9.21WKK160 pKa = 8.08TFCNTLCC167 pKa = 4.58

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

480

167

313

240.0

27.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.792 ± 1.006

2.292 ± 0.729

4.583 ± 1.562

6.667 ± 1.387

3.75 ± 0.759

6.667 ± 0.626

2.708 ± 0.496

4.167 ± 0.014

6.042 ± 0.366

10.625 ± 1.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.875 ± 0.627

4.167 ± 0.014

3.542 ± 0.643

3.542 ± 0.978

7.917 ± 0.933

9.583 ± 0.67

5.0 ± 1.56

5.833 ± 2.262

2.292 ± 0.394

3.958 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski