Amino acid dipepetide frequency for Escherichia phage vB_EcoS-IME253

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.795AlaAla: 7.795 ± 1.011
0.715AlaCys: 0.715 ± 0.233
4.577AlaAsp: 4.577 ± 0.494
5.077AlaGlu: 5.077 ± 0.721
3.289AlaPhe: 3.289 ± 0.466
6.364AlaGly: 6.364 ± 0.59
1.216AlaHis: 1.216 ± 0.316
6.221AlaIle: 6.221 ± 0.699
5.649AlaLys: 5.649 ± 1.075
7.58AlaLeu: 7.58 ± 1.066
1.931AlaMet: 1.931 ± 0.383
4.291AlaAsn: 4.291 ± 0.543
2.145AlaPro: 2.145 ± 0.392
3.218AlaGln: 3.218 ± 0.653
5.292AlaArg: 5.292 ± 0.598
4.505AlaSer: 4.505 ± 0.45
4.148AlaThr: 4.148 ± 0.644
5.435AlaVal: 5.435 ± 0.628
0.93AlaTrp: 0.93 ± 0.286
2.145AlaTyr: 2.145 ± 0.325
0.0AlaXaa: 0.0 ± 0.0
Cys
1.001CysAla: 1.001 ± 0.274
0.286CysCys: 0.286 ± 0.125
0.93CysAsp: 0.93 ± 0.208
0.787CysGlu: 0.787 ± 0.236
0.286CysPhe: 0.286 ± 0.131
1.287CysGly: 1.287 ± 0.357
0.215CysHis: 0.215 ± 0.117
0.501CysIle: 0.501 ± 0.222
0.858CysLys: 0.858 ± 0.275
0.644CysLeu: 0.644 ± 0.199
0.286CysMet: 0.286 ± 0.147
0.858CysAsn: 0.858 ± 0.274
0.143CysPro: 0.143 ± 0.11
0.215CysGln: 0.215 ± 0.123
1.073CysArg: 1.073 ± 0.322
1.144CysSer: 1.144 ± 0.277
0.644CysThr: 0.644 ± 0.187
0.572CysVal: 0.572 ± 0.189
0.358CysTrp: 0.358 ± 0.146
0.429CysTyr: 0.429 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
4.72AspAla: 4.72 ± 0.75
0.715AspCys: 0.715 ± 0.245
3.361AspAsp: 3.361 ± 0.662
4.291AspGlu: 4.291 ± 0.606
2.574AspPhe: 2.574 ± 0.489
7.223AspGly: 7.223 ± 0.876
0.93AspHis: 0.93 ± 0.323
4.362AspIle: 4.362 ± 0.568
4.505AspLys: 4.505 ± 0.533
4.648AspLeu: 4.648 ± 0.584
1.359AspMet: 1.359 ± 0.338
2.646AspAsn: 2.646 ± 0.523
2.002AspPro: 2.002 ± 0.333
1.073AspGln: 1.073 ± 0.259
1.859AspArg: 1.859 ± 0.494
3.576AspSer: 3.576 ± 0.603
2.574AspThr: 2.574 ± 0.411
4.291AspVal: 4.291 ± 0.501
1.073AspTrp: 1.073 ± 0.302
2.86AspTyr: 2.86 ± 0.42
0.0AspXaa: 0.0 ± 0.0
Glu
5.578GluAla: 5.578 ± 0.723
1.001GluCys: 1.001 ± 0.284
3.289GluAsp: 3.289 ± 0.522
4.362GluGlu: 4.362 ± 0.657
3.146GluPhe: 3.146 ± 0.59
3.647GluGly: 3.647 ± 0.488
0.572GluHis: 0.572 ± 0.193
4.72GluIle: 4.72 ± 0.503
3.719GluLys: 3.719 ± 0.624
6.078GluLeu: 6.078 ± 0.762
2.932GluMet: 2.932 ± 0.532
3.218GluAsn: 3.218 ± 0.513
1.859GluPro: 1.859 ± 0.357
3.218GluGln: 3.218 ± 0.639
3.289GluArg: 3.289 ± 0.55
4.791GluSer: 4.791 ± 0.648
3.218GluThr: 3.218 ± 0.443
5.077GluVal: 5.077 ± 0.655
0.572GluTrp: 0.572 ± 0.187
2.503GluTyr: 2.503 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
2.288PheAla: 2.288 ± 0.365
1.001PheCys: 1.001 ± 0.31
2.932PheAsp: 2.932 ± 0.472
3.289PheGlu: 3.289 ± 0.63
1.216PhePhe: 1.216 ± 0.293
3.003PheGly: 3.003 ± 0.467
0.93PheHis: 0.93 ± 0.245
2.217PheIle: 2.217 ± 0.303
2.574PheLys: 2.574 ± 0.443
2.217PheLeu: 2.217 ± 0.377
1.001PheMet: 1.001 ± 0.277
2.36PheAsn: 2.36 ± 0.474
1.144PhePro: 1.144 ± 0.29
1.573PheGln: 1.573 ± 0.384
2.002PheArg: 2.002 ± 0.432
2.431PheSer: 2.431 ± 0.403
2.646PheThr: 2.646 ± 0.344
2.646PheVal: 2.646 ± 0.45
0.286PheTrp: 0.286 ± 0.122
1.073PheTyr: 1.073 ± 0.227
0.0PheXaa: 0.0 ± 0.0
Gly
4.863GlyAla: 4.863 ± 0.756
1.645GlyCys: 1.645 ± 0.391
4.076GlyAsp: 4.076 ± 0.583
4.934GlyGlu: 4.934 ± 0.557
2.646GlyPhe: 2.646 ± 0.335
4.863GlyGly: 4.863 ± 0.948
0.787GlyHis: 0.787 ± 0.304
5.292GlyIle: 5.292 ± 0.544
6.007GlyLys: 6.007 ± 0.834
6.65GlyLeu: 6.65 ± 0.662
2.002GlyMet: 2.002 ± 0.455
3.432GlyAsn: 3.432 ± 0.583
0.644GlyPro: 0.644 ± 0.24
2.074GlyGln: 2.074 ± 0.369
2.86GlyArg: 2.86 ± 0.379
5.149GlySer: 5.149 ± 0.775
3.862GlyThr: 3.862 ± 0.599
5.721GlyVal: 5.721 ± 0.583
1.573GlyTrp: 1.573 ± 0.395
4.291GlyTyr: 4.291 ± 0.554
0.0GlyXaa: 0.0 ± 0.0
His
0.644HisAla: 0.644 ± 0.244
0.358HisCys: 0.358 ± 0.169
0.572HisAsp: 0.572 ± 0.202
1.073HisGlu: 1.073 ± 0.318
0.501HisPhe: 0.501 ± 0.185
1.001HisGly: 1.001 ± 0.317
0.286HisHis: 0.286 ± 0.152
1.287HisIle: 1.287 ± 0.285
1.359HisLys: 1.359 ± 0.35
1.216HisLeu: 1.216 ± 0.279
0.501HisMet: 0.501 ± 0.205
0.429HisAsn: 0.429 ± 0.198
0.143HisPro: 0.143 ± 0.093
0.572HisGln: 0.572 ± 0.215
0.715HisArg: 0.715 ± 0.229
0.715HisSer: 0.715 ± 0.218
0.715HisThr: 0.715 ± 0.233
1.073HisVal: 1.073 ± 0.258
0.143HisTrp: 0.143 ± 0.1
0.572HisTyr: 0.572 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
7.08IleAla: 7.08 ± 0.94
0.572IleCys: 0.572 ± 0.2
5.935IleAsp: 5.935 ± 0.567
3.719IleGlu: 3.719 ± 0.44
1.788IlePhe: 1.788 ± 0.329
4.076IleGly: 4.076 ± 0.554
1.001IleHis: 1.001 ± 0.28
3.504IleIle: 3.504 ± 0.616
5.292IleLys: 5.292 ± 0.619
3.218IleLeu: 3.218 ± 0.497
1.502IleMet: 1.502 ± 0.341
4.005IleAsn: 4.005 ± 0.63
2.717IlePro: 2.717 ± 0.495
2.288IleGln: 2.288 ± 0.423
2.932IleArg: 2.932 ± 0.361
6.436IleSer: 6.436 ± 0.974
5.006IleThr: 5.006 ± 0.557
4.005IleVal: 4.005 ± 0.498
0.644IleTrp: 0.644 ± 0.185
2.789IleTyr: 2.789 ± 0.612
0.0IleXaa: 0.0 ± 0.0
Lys
6.293LysAla: 6.293 ± 0.915
0.572LysCys: 0.572 ± 0.222
3.79LysAsp: 3.79 ± 0.487
5.506LysGlu: 5.506 ± 0.881
3.218LysPhe: 3.218 ± 0.488
2.86LysGly: 2.86 ± 0.499
1.073LysHis: 1.073 ± 0.286
4.505LysIle: 4.505 ± 0.468
4.291LysLys: 4.291 ± 0.616
4.934LysLeu: 4.934 ± 0.664
3.146LysMet: 3.146 ± 0.543
2.646LysAsn: 2.646 ± 0.5
1.716LysPro: 1.716 ± 0.372
2.431LysGln: 2.431 ± 0.382
3.075LysArg: 3.075 ± 0.56
4.791LysSer: 4.791 ± 0.685
3.862LysThr: 3.862 ± 0.475
4.934LysVal: 4.934 ± 0.637
0.787LysTrp: 0.787 ± 0.285
2.86LysTyr: 2.86 ± 0.479
0.0LysXaa: 0.0 ± 0.0
Leu
6.293LeuAla: 6.293 ± 0.835
1.001LeuCys: 1.001 ± 0.212
4.791LeuAsp: 4.791 ± 0.535
4.005LeuGlu: 4.005 ± 0.613
2.646LeuPhe: 2.646 ± 0.403
4.72LeuGly: 4.72 ± 0.638
0.93LeuHis: 0.93 ± 0.28
5.935LeuIle: 5.935 ± 0.588
4.72LeuLys: 4.72 ± 0.694
3.862LeuLeu: 3.862 ± 0.42
1.001LeuMet: 1.001 ± 0.265
3.719LeuAsn: 3.719 ± 0.45
2.574LeuPro: 2.574 ± 0.426
2.646LeuGln: 2.646 ± 0.838
3.504LeuArg: 3.504 ± 0.495
6.364LeuSer: 6.364 ± 0.675
4.291LeuThr: 4.291 ± 0.534
5.649LeuVal: 5.649 ± 0.488
0.572LeuTrp: 0.572 ± 0.261
2.145LeuTyr: 2.145 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
3.146MetAla: 3.146 ± 0.464
0.143MetCys: 0.143 ± 0.092
0.715MetAsp: 0.715 ± 0.213
1.716MetGlu: 1.716 ± 0.423
1.073MetPhe: 1.073 ± 0.267
1.001MetGly: 1.001 ± 0.25
0.429MetHis: 0.429 ± 0.195
1.931MetIle: 1.931 ± 0.377
1.645MetLys: 1.645 ± 0.398
1.859MetLeu: 1.859 ± 0.391
0.715MetMet: 0.715 ± 0.272
1.859MetAsn: 1.859 ± 0.441
0.358MetPro: 0.358 ± 0.146
1.216MetGln: 1.216 ± 0.312
1.502MetArg: 1.502 ± 0.3
1.502MetSer: 1.502 ± 0.293
2.145MetThr: 2.145 ± 0.422
1.645MetVal: 1.645 ± 0.364
0.286MetTrp: 0.286 ± 0.142
0.787MetTyr: 0.787 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
3.933AsnAla: 3.933 ± 0.511
0.572AsnCys: 0.572 ± 0.296
3.289AsnAsp: 3.289 ± 0.386
4.005AsnGlu: 4.005 ± 0.645
2.074AsnPhe: 2.074 ± 0.3
5.435AsnGly: 5.435 ± 1.031
0.787AsnHis: 0.787 ± 0.228
3.146AsnIle: 3.146 ± 0.4
3.003AsnLys: 3.003 ± 0.465
3.289AsnLeu: 3.289 ± 0.539
1.502AsnMet: 1.502 ± 0.314
2.932AsnAsn: 2.932 ± 0.453
1.788AsnPro: 1.788 ± 0.299
2.36AsnGln: 2.36 ± 0.545
2.074AsnArg: 2.074 ± 0.365
4.362AsnSer: 4.362 ± 0.685
1.788AsnThr: 1.788 ± 0.266
4.005AsnVal: 4.005 ± 0.491
1.073AsnTrp: 1.073 ± 0.252
1.788AsnTyr: 1.788 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
2.86ProAla: 2.86 ± 0.365
0.429ProCys: 0.429 ± 0.203
1.931ProAsp: 1.931 ± 0.484
2.717ProGlu: 2.717 ± 0.505
1.287ProPhe: 1.287 ± 0.227
1.716ProGly: 1.716 ± 0.3
0.358ProHis: 0.358 ± 0.159
1.716ProIle: 1.716 ± 0.308
1.287ProLys: 1.287 ± 0.306
1.859ProLeu: 1.859 ± 0.412
0.501ProMet: 0.501 ± 0.186
1.716ProAsn: 1.716 ± 0.347
0.715ProPro: 0.715 ± 0.23
1.287ProGln: 1.287 ± 0.343
1.144ProArg: 1.144 ± 0.265
1.287ProSer: 1.287 ± 0.333
1.573ProThr: 1.573 ± 0.338
3.289ProVal: 3.289 ± 0.56
0.501ProTrp: 0.501 ± 0.204
1.287ProTyr: 1.287 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
3.862GlnAla: 3.862 ± 0.942
0.429GlnCys: 0.429 ± 0.192
1.502GlnAsp: 1.502 ± 0.329
2.789GlnGlu: 2.789 ± 0.491
1.287GlnPhe: 1.287 ± 0.305
2.145GlnGly: 2.145 ± 0.357
0.429GlnHis: 0.429 ± 0.193
3.361GlnIle: 3.361 ± 0.676
2.002GlnLys: 2.002 ± 0.373
2.431GlnLeu: 2.431 ± 0.5
0.858GlnMet: 0.858 ± 0.236
1.931GlnAsn: 1.931 ± 0.423
1.216GlnPro: 1.216 ± 0.322
2.932GlnGln: 2.932 ± 1.34
2.074GlnArg: 2.074 ± 0.455
2.717GlnSer: 2.717 ± 0.527
1.287GlnThr: 1.287 ± 0.337
2.503GlnVal: 2.503 ± 0.37
0.358GlnTrp: 0.358 ± 0.175
1.287GlnTyr: 1.287 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
3.933ArgAla: 3.933 ± 0.534
0.572ArgCys: 0.572 ± 0.312
2.503ArgAsp: 2.503 ± 0.524
3.361ArgGlu: 3.361 ± 0.578
2.288ArgPhe: 2.288 ± 0.365
3.003ArgGly: 3.003 ± 0.412
0.715ArgHis: 0.715 ± 0.246
3.719ArgIle: 3.719 ± 0.489
5.006ArgLys: 5.006 ± 0.642
4.148ArgLeu: 4.148 ± 0.394
1.144ArgMet: 1.144 ± 0.312
2.36ArgAsn: 2.36 ± 0.405
1.43ArgPro: 1.43 ± 0.342
1.502ArgGln: 1.502 ± 0.369
2.431ArgArg: 2.431 ± 0.407
2.574ArgSer: 2.574 ± 0.507
2.074ArgThr: 2.074 ± 0.444
3.289ArgVal: 3.289 ± 0.51
0.644ArgTrp: 0.644 ± 0.175
2.145ArgTyr: 2.145 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
5.792SerAla: 5.792 ± 0.698
0.572SerCys: 0.572 ± 0.187
5.363SerAsp: 5.363 ± 0.553
4.362SerGlu: 4.362 ± 0.503
3.003SerPhe: 3.003 ± 0.443
6.436SerGly: 6.436 ± 0.793
1.073SerHis: 1.073 ± 0.255
4.577SerIle: 4.577 ± 0.456
3.361SerLys: 3.361 ± 0.528
5.006SerLeu: 5.006 ± 0.71
1.645SerMet: 1.645 ± 0.325
3.432SerAsn: 3.432 ± 0.46
2.574SerPro: 2.574 ± 0.411
2.288SerGln: 2.288 ± 0.409
3.719SerArg: 3.719 ± 0.655
5.792SerSer: 5.792 ± 1.186
3.719SerThr: 3.719 ± 0.721
5.22SerVal: 5.22 ± 0.64
0.501SerTrp: 0.501 ± 0.211
2.431SerTyr: 2.431 ± 0.429
0.0SerXaa: 0.0 ± 0.0
Thr
4.362ThrAla: 4.362 ± 0.657
0.429ThrCys: 0.429 ± 0.182
2.431ThrAsp: 2.431 ± 0.463
2.431ThrGlu: 2.431 ± 0.458
2.217ThrPhe: 2.217 ± 0.345
6.221ThrGly: 6.221 ± 0.703
0.429ThrHis: 0.429 ± 0.219
4.362ThrIle: 4.362 ± 0.497
2.503ThrLys: 2.503 ± 0.456
3.218ThrLeu: 3.218 ± 0.493
0.858ThrMet: 0.858 ± 0.241
3.576ThrAsn: 3.576 ± 0.537
2.217ThrPro: 2.217 ± 0.383
2.431ThrGln: 2.431 ± 0.45
2.431ThrArg: 2.431 ± 0.409
4.076ThrSer: 4.076 ± 0.549
3.647ThrThr: 3.647 ± 0.627
4.076ThrVal: 4.076 ± 0.499
0.429ThrTrp: 0.429 ± 0.155
2.574ThrTyr: 2.574 ± 0.456
0.0ThrXaa: 0.0 ± 0.0
Val
4.863ValAla: 4.863 ± 0.535
0.858ValCys: 0.858 ± 0.232
5.149ValAsp: 5.149 ± 0.555
4.791ValGlu: 4.791 ± 0.834
2.503ValPhe: 2.503 ± 0.38
4.791ValGly: 4.791 ± 0.651
1.001ValHis: 1.001 ± 0.263
3.79ValIle: 3.79 ± 0.548
6.221ValLys: 6.221 ± 0.588
4.648ValLeu: 4.648 ± 0.648
1.788ValMet: 1.788 ± 0.371
5.006ValAsn: 5.006 ± 0.468
2.145ValPro: 2.145 ± 0.429
2.217ValGln: 2.217 ± 0.577
3.862ValArg: 3.862 ± 0.523
5.006ValSer: 5.006 ± 0.53
4.362ValThr: 4.362 ± 0.688
5.506ValVal: 5.506 ± 0.694
1.001ValTrp: 1.001 ± 0.292
2.86ValTyr: 2.86 ± 0.458
0.0ValXaa: 0.0 ± 0.0
Trp
0.715TrpAla: 0.715 ± 0.194
0.072TrpCys: 0.072 ± 0.071
0.787TrpAsp: 0.787 ± 0.173
0.787TrpGlu: 0.787 ± 0.316
0.787TrpPhe: 0.787 ± 0.244
1.001TrpGly: 1.001 ± 0.222
0.215TrpHis: 0.215 ± 0.106
1.001TrpIle: 1.001 ± 0.264
1.216TrpLys: 1.216 ± 0.277
1.216TrpLeu: 1.216 ± 0.249
0.286TrpMet: 0.286 ± 0.142
0.358TrpAsn: 0.358 ± 0.122
0.358TrpPro: 0.358 ± 0.17
0.358TrpGln: 0.358 ± 0.139
0.644TrpArg: 0.644 ± 0.219
0.858TrpSer: 0.858 ± 0.343
0.572TrpThr: 0.572 ± 0.189
0.644TrpVal: 0.644 ± 0.263
0.215TrpTrp: 0.215 ± 0.124
0.501TrpTyr: 0.501 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.503TyrAla: 2.503 ± 0.428
0.572TyrCys: 0.572 ± 0.186
2.717TyrAsp: 2.717 ± 0.476
2.789TyrGlu: 2.789 ± 0.384
1.001TyrPhe: 1.001 ± 0.289
2.574TyrGly: 2.574 ± 0.394
0.501TyrHis: 0.501 ± 0.166
2.36TyrIle: 2.36 ± 0.395
2.145TyrLys: 2.145 ± 0.427
2.646TyrLeu: 2.646 ± 0.398
0.644TyrMet: 0.644 ± 0.221
2.431TyrAsn: 2.431 ± 0.452
1.573TyrPro: 1.573 ± 0.365
1.502TyrGln: 1.502 ± 0.381
2.36TyrArg: 2.36 ± 0.399
2.789TyrSer: 2.789 ± 0.44
2.789TyrThr: 2.789 ± 0.38
2.789TyrVal: 2.789 ± 0.402
0.644TyrTrp: 0.644 ± 0.204
1.645TyrTyr: 1.645 ± 0.396
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (13985 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski