Amino acid dipepetide frequency for Chara braunii (Braun s stonewort)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.654AlaAla: 10.654 ± 0.054	1.239AlaCys: 1.239 ± 0.009	4.469AlaAsp: 4.469 ± 0.014	6.14AlaGlu: 6.14 ± 0.024	2.38AlaPhe: 2.38 ± 0.012	6.498AlaGly: 6.498 ± 0.026	1.564AlaHis: 1.564 ± 0.01	3.17AlaIle: 3.17 ± 0.014	4.024AlaLys: 4.024 ± 0.018	6.534AlaLeu: 6.534 ± 0.019	2.503AlaMet: 2.503 ± 0.011	2.394AlaAsn: 2.394 ± 0.011	4.293AlaPro: 4.293 ± 0.02	3.203AlaGln: 3.203 ± 0.016	5.927AlaArg: 5.927 ± 0.016	6.264AlaSer: 6.264 ± 0.023	4.905AlaThr: 4.905 ± 0.016	6.07AlaVal: 6.07 ± 0.022	0.903AlaTrp: 0.903 ± 0.007	1.486AlaTyr: 1.486 ± 0.009	0.052AlaXaa: 0.052 ± 0.002
Cys	1.231CysAla: 1.231 ± 0.01	0.346CysCys: 0.346 ± 0.005	0.859CysAsp: 0.859 ± 0.008	1.065CysGlu: 1.065 ± 0.008	0.494CysPhe: 0.494 ± 0.006	1.307CysGly: 1.307 ± 0.01	0.509CysHis: 0.509 ± 0.005	0.792CysIle: 0.792 ± 0.006	0.722CysLys: 0.722 ± 0.006	1.132CysLeu: 1.132 ± 0.008	0.383CysMet: 0.383 ± 0.004	0.581CysAsn: 0.581 ± 0.006	0.917CysPro: 0.917 ± 0.008	0.675CysGln: 0.675 ± 0.005	1.201CysArg: 1.201 ± 0.008	1.252CysSer: 1.252 ± 0.008	0.959CysThr: 0.959 ± 0.008	0.986CysVal: 0.986 ± 0.007	0.208CysTrp: 0.208 ± 0.003	0.327CysTyr: 0.327 ± 0.004	0.009CysXaa: 0.009 ± 0.001
Asp	4.694AspAla: 4.694 ± 0.014	0.896AspCys: 0.896 ± 0.007	6.555AspAsp: 6.555 ± 0.058	5.138AspGlu: 5.138 ± 0.033	1.943AspPhe: 1.943 ± 0.01	5.57AspGly: 5.57 ± 0.029	1.462AspHis: 1.462 ± 0.01	2.696AspIle: 2.696 ± 0.011	2.74AspLys: 2.74 ± 0.013	4.935AspLeu: 4.935 ± 0.019	1.341AspMet: 1.341 ± 0.008	1.901AspAsn: 1.901 ± 0.018	2.984AspPro: 2.984 ± 0.011	1.905AspGln: 1.905 ± 0.01	4.269AspArg: 4.269 ± 0.017	3.285AspSer: 3.285 ± 0.015	2.464AspThr: 2.464 ± 0.012	4.149AspVal: 4.149 ± 0.018	1.129AspTrp: 1.129 ± 0.008	1.359AspTyr: 1.359 ± 0.008	0.042AspXaa: 0.042 ± 0.002
Glu	6.612GluAla: 6.612 ± 0.026	0.897GluCys: 0.897 ± 0.006	5.382GluAsp: 5.382 ± 0.03	13.554GluGlu: 13.554 ± 0.109	2.219GluPhe: 2.219 ± 0.01	6.721GluGly: 6.721 ± 0.029	1.58GluHis: 1.58 ± 0.009	2.728GluIle: 2.728 ± 0.014	4.671GluLys: 4.671 ± 0.026	5.506GluLeu: 5.506 ± 0.022	1.767GluMet: 1.767 ± 0.012	1.85GluAsn: 1.85 ± 0.009	2.782GluPro: 2.782 ± 0.014	2.999GluGln: 2.999 ± 0.013	5.966GluArg: 5.966 ± 0.026	3.901GluSer: 3.901 ± 0.019	3.092GluThr: 3.092 ± 0.017	5.409GluVal: 5.409 ± 0.02	1.24GluTrp: 1.24 ± 0.011	1.536GluTyr: 1.536 ± 0.008	0.04GluXaa: 0.04 ± 0.002
Phe	2.323PheAla: 2.323 ± 0.011	0.576PheCys: 0.576 ± 0.006	2.045PheAsp: 2.045 ± 0.011	1.862PheGlu: 1.862 ± 0.009	1.106PhePhe: 1.106 ± 0.009	2.263PheGly: 2.263 ± 0.013	0.809PheHis: 0.809 ± 0.005	1.335PheIle: 1.335 ± 0.009	1.361PheLys: 1.361 ± 0.008	2.803PheLeu: 2.803 ± 0.015	0.781PheMet: 0.781 ± 0.006	0.805PheAsn: 0.805 ± 0.007	1.313PhePro: 1.313 ± 0.009	1.209PheGln: 1.209 ± 0.007	1.968PheArg: 1.968 ± 0.01	2.117PheSer: 2.117 ± 0.011	1.461PheThr: 1.461 ± 0.009	2.451PheVal: 2.451 ± 0.012	0.492PheTrp: 0.492 ± 0.004	0.734PheTyr: 0.734 ± 0.005	0.021PheXaa: 0.021 ± 0.001
Gly	6.097GlyAla: 6.097 ± 0.024	1.324GlyCys: 1.324 ± 0.009	4.804GlyAsp: 4.804 ± 0.034	6.215GlyGlu: 6.215 ± 0.028	1.904GlyPhe: 1.904 ± 0.01	12.421GlyGly: 12.421 ± 0.085	2.073GlyHis: 2.073 ± 0.013	2.585GlyIle: 2.585 ± 0.012	4.111GlyLys: 4.111 ± 0.025	5.284GlyLeu: 5.284 ± 0.018	1.996GlyMet: 1.996 ± 0.012	2.422GlyAsn: 2.422 ± 0.013	3.385GlyPro: 3.385 ± 0.019	2.839GlyGln: 2.839 ± 0.019	6.772GlyArg: 6.772 ± 0.035	6.111GlySer: 6.111 ± 0.027	3.82GlyThr: 3.82 ± 0.018	5.286GlyVal: 5.286 ± 0.021	1.165GlyTrp: 1.165 ± 0.014	1.97GlyTyr: 1.97 ± 0.011	0.046GlyXaa: 0.046 ± 0.002
His	1.804HisAla: 1.804 ± 0.009	0.5HisCys: 0.5 ± 0.005	1.38HisAsp: 1.38 ± 0.01	1.436HisGlu: 1.436 ± 0.013	1.015HisPhe: 1.015 ± 0.007	1.795HisGly: 1.795 ± 0.011	0.844HisHis: 0.844 ± 0.01	0.906HisIle: 0.906 ± 0.007	1.029HisLys: 1.029 ± 0.008	2.278HisLeu: 2.278 ± 0.012	0.549HisMet: 0.549 ± 0.005	0.646HisAsn: 0.646 ± 0.006	1.505HisPro: 1.505 ± 0.01	1.099HisGln: 1.099 ± 0.008	1.611HisArg: 1.611 ± 0.012	1.443HisSer: 1.443 ± 0.009	1.15HisThr: 1.15 ± 0.007	1.807HisVal: 1.807 ± 0.01	0.363HisTrp: 0.363 ± 0.004	0.726HisTyr: 0.726 ± 0.006	0.016HisXaa: 0.016 ± 0.001
Ile	3.253IleAla: 3.253 ± 0.014	0.606IleCys: 0.606 ± 0.005	2.739IleAsp: 2.739 ± 0.013	2.645IleGlu: 2.645 ± 0.014	1.161IlePhe: 1.161 ± 0.008	2.515IleGly: 2.515 ± 0.012	0.851IleHis: 0.851 ± 0.006	1.472IleIle: 1.472 ± 0.009	1.703IleLys: 1.703 ± 0.01	3.29IleLeu: 3.29 ± 0.014	0.741IleMet: 0.741 ± 0.006	1.123IleAsn: 1.123 ± 0.007	2.103IlePro: 2.103 ± 0.012	1.344IleGln: 1.344 ± 0.008	2.94IleArg: 2.94 ± 0.013	2.679IleSer: 2.679 ± 0.01	1.902IleThr: 1.902 ± 0.01	2.617IleVal: 2.617 ± 0.012	0.485IleTrp: 0.485 ± 0.005	0.842IleTyr: 0.842 ± 0.006	0.024IleXaa: 0.024 ± 0.001
Lys	4.072LysAla: 4.072 ± 0.019	0.797LysCys: 0.797 ± 0.006	2.82LysAsp: 2.82 ± 0.015	4.917LysGlu: 4.917 ± 0.028	1.437LysPhe: 1.437 ± 0.008	3.858LysGly: 3.858 ± 0.022	1.019LysHis: 1.019 ± 0.007	2.018LysIle: 2.018 ± 0.013	4.595LysLys: 4.595 ± 0.049	4.192LysLeu: 4.192 ± 0.018	1.274LysMet: 1.274 ± 0.009	1.449LysAsn: 1.449 ± 0.009	1.989LysPro: 1.989 ± 0.01	1.74LysGln: 1.74 ± 0.011	4.001LysArg: 4.001 ± 0.017	2.934LysSer: 2.934 ± 0.015	2.895LysThr: 2.895 ± 0.014	3.325LysVal: 3.325 ± 0.016	0.808LysTrp: 0.808 ± 0.007	1.136LysTyr: 1.136 ± 0.011	0.029LysXaa: 0.029 ± 0.001
Leu	6.588LeuAla: 6.588 ± 0.019	1.357LeuCys: 1.357 ± 0.008	4.87LeuAsp: 4.87 ± 0.019	5.964LeuGlu: 5.964 ± 0.024	2.215LeuPhe: 2.215 ± 0.011	5.469LeuGly: 5.469 ± 0.016	2.26LeuHis: 2.26 ± 0.01	2.662LeuIle: 2.662 ± 0.012	4.239LeuLys: 4.239 ± 0.019	7.446LeuLeu: 7.446 ± 0.027	1.7LeuMet: 1.7 ± 0.01	1.918LeuAsn: 1.918 ± 0.011	4.681LeuPro: 4.681 ± 0.018	3.855LeuGln: 3.855 ± 0.018	6.788LeuArg: 6.788 ± 0.021	5.755LeuSer: 5.755 ± 0.017	4.303LeuThr: 4.303 ± 0.017	5.119LeuVal: 5.119 ± 0.022	1.049LeuTrp: 1.049 ± 0.009	1.713LeuTyr: 1.713 ± 0.009	0.045LeuXaa: 0.045 ± 0.001
Met	2.28MetAla: 2.28 ± 0.013	0.327MetCys: 0.327 ± 0.004	1.663MetAsp: 1.663 ± 0.011	2.022MetGlu: 2.022 ± 0.015	0.55MetPhe: 0.55 ± 0.005	1.703MetGly: 1.703 ± 0.013	0.478MetHis: 0.478 ± 0.004	0.839MetIle: 0.839 ± 0.007	1.262MetLys: 1.262 ± 0.009	1.757MetLeu: 1.757 ± 0.009	0.875MetMet: 0.875 ± 0.013	0.736MetAsn: 0.736 ± 0.006	1.394MetPro: 1.394 ± 0.008	1.007MetGln: 1.007 ± 0.008	1.719MetArg: 1.719 ± 0.009	1.676MetSer: 1.676 ± 0.007	1.429MetThr: 1.429 ± 0.008	1.59MetVal: 1.59 ± 0.01	0.308MetTrp: 0.308 ± 0.004	0.449MetTyr: 0.449 ± 0.004	0.013MetXaa: 0.013 ± 0.001
Asn	2.38AsnAla: 2.38 ± 0.012	0.491AsnCys: 0.491 ± 0.005	1.688AsnAsp: 1.688 ± 0.012	1.72AsnGlu: 1.72 ± 0.012	1.033AsnPhe: 1.033 ± 0.007	2.326AsnGly: 2.326 ± 0.016	0.678AsnHis: 0.678 ± 0.006	1.082AsnIle: 1.082 ± 0.008	1.527AsnLys: 1.527 ± 0.011	2.344AsnLeu: 2.344 ± 0.011	0.664AsnMet: 0.664 ± 0.006	1.502AsnAsn: 1.502 ± 0.033	1.354AsnPro: 1.354 ± 0.008	0.979AsnGln: 0.979 ± 0.008	2.015AsnArg: 2.015 ± 0.01	1.813AsnSer: 1.813 ± 0.011	1.317AsnThr: 1.317 ± 0.008	1.933AsnVal: 1.933 ± 0.01	0.466AsnTrp: 0.466 ± 0.005	0.772AsnTyr: 0.772 ± 0.006	0.015AsnXaa: 0.015 ± 0.001
Pro	4.338ProAla: 4.338 ± 0.021	0.875ProCys: 0.875 ± 0.008	2.791ProAsp: 2.791 ± 0.012	3.055ProGlu: 3.055 ± 0.014	1.752ProPhe: 1.752 ± 0.009	3.412ProGly: 3.412 ± 0.02	1.215ProHis: 1.215 ± 0.008	1.85ProIle: 1.85 ± 0.01	2.22ProLys: 2.22 ± 0.012	4.335ProLeu: 4.335 ± 0.022	1.021ProMet: 1.021 ± 0.007	1.292ProAsn: 1.292 ± 0.009	4.862ProPro: 4.862 ± 0.063	2.095ProGln: 2.095 ± 0.014	3.945ProArg: 3.945 ± 0.016	5.194ProSer: 5.194 ± 0.023	3.189ProThr: 3.189 ± 0.016	3.632ProVal: 3.632 ± 0.013	0.856ProTrp: 0.856 ± 0.007	1.082ProTyr: 1.082 ± 0.008	0.041ProXaa: 0.041 ± 0.002
Gln	3.674GlnAla: 3.674 ± 0.017	0.616GlnCys: 0.616 ± 0.006	1.894GlnAsp: 1.894 ± 0.01	3.152GlnGlu: 3.152 ± 0.016	1.051GlnPhe: 1.051 ± 0.007	3.1GlnGly: 3.1 ± 0.021	1.243GlnHis: 1.243 ± 0.009	1.322GlnIle: 1.322 ± 0.008	1.867GlnLys: 1.867 ± 0.011	3.399GlnLeu: 3.399 ± 0.015	0.909GlnMet: 0.909 ± 0.007	0.827GlnAsn: 0.827 ± 0.009	2.1GlnPro: 2.1 ± 0.015	3.446GlnGln: 3.446 ± 0.051	3.509GlnArg: 3.509 ± 0.017	2.34GlnSer: 2.34 ± 0.015	2.078GlnThr: 2.078 ± 0.01	2.562GlnVal: 2.562 ± 0.014	0.616GlnTrp: 0.616 ± 0.005	0.879GlnTyr: 0.879 ± 0.008	0.024GlnXaa: 0.024 ± 0.001
Arg	5.459ArgAla: 5.459 ± 0.018	1.169ArgCys: 1.169 ± 0.01	4.182ArgAsp: 4.182 ± 0.018	5.985ArgGlu: 5.985 ± 0.023	2.353ArgPhe: 2.353 ± 0.011	6.123ArgGly: 6.123 ± 0.032	1.949ArgHis: 1.949 ± 0.01	2.76ArgIle: 2.76 ± 0.012	4.42ArgLys: 4.42 ± 0.02	6.369ArgLeu: 6.369 ± 0.019	2.04ArgMet: 2.04 ± 0.011	2.182ArgAsn: 2.182 ± 0.011	4.028ArgPro: 4.028 ± 0.016	3.449ArgGln: 3.449 ± 0.018	7.923ArgArg: 7.923 ± 0.037	5.522ArgSer: 5.522 ± 0.019	3.874ArgThr: 3.874 ± 0.016	4.459ArgVal: 4.459 ± 0.015	1.418ArgTrp: 1.418 ± 0.009	1.554ArgTyr: 1.554 ± 0.009	0.042ArgXaa: 0.042 ± 0.001
Ser	5.995SerAla: 5.995 ± 0.023	1.296SerCys: 1.296 ± 0.01	3.823SerAsp: 3.823 ± 0.016	4.357SerGlu: 4.357 ± 0.016	2.303SerPhe: 2.303 ± 0.011	6.152SerGly: 6.152 ± 0.026	1.521SerHis: 1.521 ± 0.009	2.579SerIle: 2.579 ± 0.012	3.228SerLys: 3.228 ± 0.014	5.272SerLeu: 5.272 ± 0.02	1.587SerMet: 1.587 ± 0.01	1.86SerAsn: 1.86 ± 0.013	4.559SerPro: 4.559 ± 0.024	2.844SerGln: 2.844 ± 0.014	5.338SerArg: 5.338 ± 0.017	8.609SerSer: 8.609 ± 0.057	4.384SerThr: 4.384 ± 0.017	4.046SerVal: 4.046 ± 0.015	0.907SerTrp: 0.907 ± 0.007	1.391SerTyr: 1.391 ± 0.009	0.04SerXaa: 0.04 ± 0.002
Thr	4.472ThrAla: 4.472 ± 0.016	0.902ThrCys: 0.902 ± 0.007	3.111ThrAsp: 3.111 ± 0.013	3.294ThrGlu: 3.294 ± 0.015	1.707ThrPhe: 1.707 ± 0.008	3.741ThrGly: 3.741 ± 0.016	1.051ThrHis: 1.051 ± 0.007	2.161ThrIle: 2.161 ± 0.011	2.379ThrLys: 2.379 ± 0.012	4.171ThrLeu: 4.171 ± 0.016	1.254ThrMet: 1.254 ± 0.007	1.554ThrAsn: 1.554 ± 0.009	3.279ThrPro: 3.279 ± 0.019	1.982ThrGln: 1.982 ± 0.012	3.763ThrArg: 3.763 ± 0.016	4.311ThrSer: 4.311 ± 0.018	3.719ThrThr: 3.719 ± 0.02	3.565ThrVal: 3.565 ± 0.015	0.828ThrTrp: 0.828 ± 0.01	1.098ThrTyr: 1.098 ± 0.009	0.041ThrXaa: 0.041 ± 0.002
Val	6.25ValAla: 6.25 ± 0.022	1.116ValCys: 1.116 ± 0.007	4.145ValAsp: 4.145 ± 0.016	5.402ValGlu: 5.402 ± 0.024	1.939ValPhe: 1.939 ± 0.012	4.893ValGly: 4.893 ± 0.023	1.665ValHis: 1.665 ± 0.008	2.444ValIle: 2.444 ± 0.012	3.082ValLys: 3.082 ± 0.018	5.675ValLeu: 5.675 ± 0.018	1.732ValMet: 1.732 ± 0.011	1.765ValAsn: 1.765 ± 0.009	3.885ValPro: 3.885 ± 0.017	2.567ValGln: 2.567 ± 0.015	4.664ValArg: 4.664 ± 0.016	4.315ValSer: 4.315 ± 0.017	3.715ValThr: 3.715 ± 0.014	6.158ValVal: 6.158 ± 0.165	0.895ValTrp: 0.895 ± 0.007	1.572ValTyr: 1.572 ± 0.009	0.043ValXaa: 0.043 ± 0.002
Trp	0.944TrpAla: 0.944 ± 0.008	0.21TrpCys: 0.21 ± 0.003	0.82TrpAsp: 0.82 ± 0.007	1.119TrpGlu: 1.119 ± 0.01	0.401TrpPhe: 0.401 ± 0.004	0.839TrpGly: 0.839 ± 0.007	0.428TrpHis: 0.428 ± 0.004	0.731TrpIle: 0.731 ± 0.006	1.002TrpLys: 1.002 ± 0.007	1.149TrpLeu: 1.149 ± 0.007	0.492TrpMet: 0.492 ± 0.006	0.475TrpAsn: 0.475 ± 0.007	0.668TrpPro: 0.668 ± 0.005	0.69TrpGln: 0.69 ± 0.006	1.259TrpArg: 1.259 ± 0.009	0.982TrpSer: 0.982 ± 0.007	0.794TrpThr: 0.794 ± 0.01	1.151TrpVal: 1.151 ± 0.008	0.36TrpTrp: 0.36 ± 0.004	0.25TrpTyr: 0.25 ± 0.004	0.008TrpXaa: 0.008 ± 0.001
Tyr	1.501TyrAla: 1.501 ± 0.007	0.405TyrCys: 0.405 ± 0.005	1.374TyrAsp: 1.374 ± 0.01	1.361TyrGlu: 1.361 ± 0.009	0.853TyrPhe: 0.853 ± 0.006	1.876TyrGly: 1.876 ± 0.012	0.688TyrHis: 0.688 ± 0.005	0.878TyrIle: 0.878 ± 0.006	1.091TyrLys: 1.091 ± 0.008	2.081TyrLeu: 2.081 ± 0.013	0.455TyrMet: 0.455 ± 0.005	0.802TyrAsn: 0.802 ± 0.006	0.939TyrPro: 0.939 ± 0.007	0.661TyrGln: 0.661 ± 0.007	1.638TyrArg: 1.638 ± 0.009	1.549TyrSer: 1.549 ± 0.01	0.902TyrThr: 0.902 ± 0.007	1.56TyrVal: 1.56 ± 0.009	0.261TyrTrp: 0.261 ± 0.003	0.736TyrTyr: 0.736 ± 0.007	0.016TyrXaa: 0.016 ± 0.001
Xaa	0.049XaaAla: 0.049 ± 0.002	0.011XaaCys: 0.011 ± 0.001	0.039XaaAsp: 0.039 ± 0.001	0.048XaaGlu: 0.048 ± 0.002	0.02XaaPhe: 0.02 ± 0.001	0.049XaaGly: 0.049 ± 0.002	0.015XaaHis: 0.015 ± 0.001	0.024XaaIle: 0.024 ± 0.001	0.03XaaLys: 0.03 ± 0.001	0.047XaaLeu: 0.047 ± 0.001	0.014XaaMet: 0.014 ± 0.001	0.017XaaAsn: 0.017 ± 0.001	0.032XaaPro: 0.032 ± 0.001	0.022XaaGln: 0.022 ± 0.001	0.046XaaArg: 0.046 ± 0.002	0.038XaaSer: 0.038 ± 0.002	0.032XaaThr: 0.032 ± 0.001	0.047XaaVal: 0.047 ± 0.002	0.011XaaTrp: 0.011 ± 0.001	0.016XaaTyr: 0.016 ± 0.001	0.264XaaXaa: 0.264 ± 0.022

Statistics based on 35576 proteins (23446944 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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