Lentibacter virus vB_LenP_ICBM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Cobavirinae; Siovirus; unclassified Siovirus

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2YRD8|A0A3G2YRD8_9CAUD Acyl-CoA N-acyltransferase (GNAT) domain protein OS=Lentibacter virus vB_LenP_ICBM2 OX=2301529 GN=vBLenPICBM2__38 PE=4 SV=1
MM1 pKa = 6.94TEE3 pKa = 4.14EE4 pKa = 4.07VGHH7 pKa = 5.83MKK9 pKa = 9.17VTDD12 pKa = 3.72VFEE15 pKa = 6.32HH16 pKa = 6.95EE17 pKa = 5.16DD18 pKa = 3.56GSATMTFDD26 pKa = 3.97MDD28 pKa = 4.08DD29 pKa = 3.27ATAALAQEE37 pKa = 4.97LGLKK41 pKa = 10.18LLIYY45 pKa = 10.6CGATGTDD52 pKa = 4.03LDD54 pKa = 4.66CVFDD58 pKa = 4.54SILGRR63 pKa = 11.84GQDD66 pKa = 3.7YY67 pKa = 9.97EE68 pKa = 4.1

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411MQW9|A0A411MQW9_9CAUD Uncharacterized protein OS=Lentibacter virus vB_LenP_ICBM2 OX=2301529 GN=vBLenPICBM2__40 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84ILIAYY7 pKa = 7.75LSRR10 pKa = 11.84VGIALSVLLNVILGGEE26 pKa = 4.34SNQTFSARR34 pKa = 11.84NYY36 pKa = 7.66SWQRR40 pKa = 11.84NRR42 pKa = 11.84RR43 pKa = 11.84PNIAKK48 pKa = 10.46AIDD51 pKa = 3.62SVLGKK56 pKa = 8.52DD57 pKa = 3.14HH58 pKa = 7.31CMLSWVHH65 pKa = 5.46WSTRR69 pKa = 11.84KK70 pKa = 10.2SKK72 pKa = 10.79GG73 pKa = 3.18

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12877

52

1484

234.1

25.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.962 ± 0.626

0.637 ± 0.137

6.803 ± 0.263

6.717 ± 0.353

3.844 ± 0.272

6.849 ± 0.377

1.475 ± 0.199

4.753 ± 0.204

5.832 ± 0.34

8.007 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.183

4.722 ± 0.185

3.665 ± 0.242

3.813 ± 0.22

4.644 ± 0.3

7.331 ± 0.546

7.183 ± 0.422

7.09 ± 0.284

1.507 ± 0.157

3.704 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski