Longibaculum muris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Longibaculum

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3Z5Q2|A0A4R3Z5Q2_9FIRM 50S ribosomal protein L11 OS=Longibaculum muris OX=1796628 GN=rplK PE=3 SV=1
MM1 pKa = 7.71LLEE4 pKa = 5.51HH5 pKa = 7.24ILDD8 pKa = 4.34CVNDD12 pKa = 3.41IYY14 pKa = 11.25EE15 pKa = 4.56DD16 pKa = 3.84VMEE19 pKa = 4.69GASPEE24 pKa = 4.05LMNVLKK30 pKa = 10.52QLEE33 pKa = 4.4EE34 pKa = 4.04EE35 pKa = 4.27AHH37 pKa = 6.67DD38 pKa = 4.43SSVDD42 pKa = 3.74LTKK45 pKa = 10.73KK46 pKa = 10.69AIMLSMTLCMSQLEE60 pKa = 4.16FDD62 pKa = 4.78DD63 pKa = 5.93DD64 pKa = 4.25LFEE67 pKa = 5.74GLLLEE72 pKa = 4.69NLDD75 pKa = 4.26HH76 pKa = 6.44FVKK79 pKa = 10.56HH80 pKa = 6.01IPDD83 pKa = 3.46MKK85 pKa = 10.0KK86 pKa = 8.89TMSLRR91 pKa = 11.84IEE93 pKa = 3.93LRR95 pKa = 11.84GLEE98 pKa = 4.19KK99 pKa = 10.76NVNRR103 pKa = 11.84VIKK106 pKa = 10.33VPYY109 pKa = 10.57GMVLADD115 pKa = 4.25LAYY118 pKa = 10.91LILASMNAEE127 pKa = 4.04GEE129 pKa = 4.23HH130 pKa = 6.66LFTFISDD137 pKa = 3.49EE138 pKa = 4.16GKK140 pKa = 10.77YY141 pKa = 10.93GCDD144 pKa = 3.09QCDD147 pKa = 3.25GEE149 pKa = 4.88MIDD152 pKa = 5.81GYY154 pKa = 11.02AADD157 pKa = 3.99MTIADD162 pKa = 4.54LSLHH166 pKa = 6.18EE167 pKa = 4.98GSHH170 pKa = 5.93LVLWYY175 pKa = 10.37DD176 pKa = 3.88FGDD179 pKa = 4.02DD180 pKa = 3.92YY181 pKa = 11.68FFDD184 pKa = 3.44IHH186 pKa = 8.29VMDD189 pKa = 3.98VDD191 pKa = 3.73EE192 pKa = 5.66HH193 pKa = 8.52NDD195 pKa = 3.3IQSLDD200 pKa = 3.9DD201 pKa = 4.19LKK203 pKa = 11.47VLAGEE208 pKa = 5.37GYY210 pKa = 10.46GIWEE214 pKa = 5.07DD215 pKa = 3.46EE216 pKa = 4.24HH217 pKa = 8.13QLLEE221 pKa = 4.55LYY223 pKa = 10.46YY224 pKa = 10.05EE225 pKa = 4.25NQEE228 pKa = 3.62EE229 pKa = 4.37FLRR232 pKa = 11.84VVSEE236 pKa = 3.97MGLNEE241 pKa = 5.08DD242 pKa = 5.26DD243 pKa = 6.5FILEE247 pKa = 4.73DD248 pKa = 5.03FDD250 pKa = 5.74VDD252 pKa = 4.27DD253 pKa = 5.29ANEE256 pKa = 3.97FLLDD260 pKa = 3.41NYY262 pKa = 10.6EE263 pKa = 4.13FLKK266 pKa = 10.3TSYY269 pKa = 10.11EE270 pKa = 4.23VYY272 pKa = 10.83DD273 pKa = 3.88EE274 pKa = 5.0DD275 pKa = 5.3VYY277 pKa = 11.94

Molecular weight: 32.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3YR90|A0A4R3YR90_9FIRM Pyridoxal phosphate homeostasis protein OS=Longibaculum muris OX=1796628 GN=EDD60_11717 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.02RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3164

0

3164

953661

27

2519

301.4

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.702 ± 0.049

1.426 ± 0.015

5.978 ± 0.035

6.846 ± 0.051

4.486 ± 0.04

5.947 ± 0.051

2.338 ± 0.025

8.868 ± 0.054

7.694 ± 0.039

9.517 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.065 ± 0.025

4.987 ± 0.037

2.869 ± 0.023

4.097 ± 0.035

3.253 ± 0.029

5.833 ± 0.038

4.989 ± 0.041

6.434 ± 0.036

0.736 ± 0.015

4.934 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski