Capybara microvirus Cap3_SP_386
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVS7|A0A4V1FVS7_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_386 OX=2585440 PE=4 SV=1
MM1 pKa = 7.34 YY2 pKa = 10.77 NFIKK6 pKa = 10.29 KK7 pKa = 10.03 RR8 pKa = 11.84 PLEE11 pKa = 4.09 QYY13 pKa = 8.14 EE14 pKa = 4.6 SPVITIQDD22 pKa = 4.05 FSTVPDD28 pKa = 3.55 EE29 pKa = 4.75 NGNKK33 pKa = 9.3 VAVFSPSTPKK43 pKa = 9.5 IDD45 pKa = 3.48 KK46 pKa = 10.83 DD47 pKa = 3.16 SDD49 pKa = 3.48 YY50 pKa = 11.72 RR51 pKa = 11.84 LMLVGNLIKK60 pKa = 10.8 SGINPVSGIFVNPLNRR76 pKa = 11.84 LSVDD80 pKa = 4.65 SIAQDD85 pKa = 3.88 YY86 pKa = 7.9 ITSLNQSINEE96 pKa = 4.19 TLL98 pKa = 3.29
Molecular weight: 10.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.914
IPC2_protein 5.041
IPC_protein 4.774
Toseland 4.596
ProMoST 4.902
Dawson 4.736
Bjellqvist 4.889
Wikipedia 4.647
Rodwell 4.609
Grimsley 4.507
Solomon 4.724
Lehninger 4.685
Nozaki 4.851
DTASelect 5.054
Thurlkill 4.622
EMBOSS 4.66
Sillero 4.889
Patrickios 4.202
IPC_peptide 4.724
IPC2_peptide 4.863
IPC2.peptide.svr19 4.958
Protein with the highest isoelectric point:
>tr|A0A4P8W599|A0A4P8W599_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_386 OX=2585440 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.1 EE3 pKa = 3.99 VITIPEE9 pKa = 4.11 VTDD12 pKa = 3.87 FSYY15 pKa = 11.02 RR16 pKa = 11.84 FNRR19 pKa = 11.84 EE20 pKa = 2.76 KK21 pKa = 11.19 DD22 pKa = 3.2 FLIRR26 pKa = 11.84 LNYY29 pKa = 10.12 EE30 pKa = 3.62 YY31 pKa = 10.82 LDD33 pKa = 4.71 CIKK36 pKa = 10.78 QGGKK40 pKa = 9.98 CLFYY44 pKa = 10.7 TLTYY48 pKa = 10.0 TNQALPKK55 pKa = 8.29 IYY57 pKa = 9.86 GVPVHH62 pKa = 7.47 DD63 pKa = 4.0 YY64 pKa = 10.01 HH65 pKa = 7.92 DD66 pKa = 3.96 LRR68 pKa = 11.84 SFVNGSFYY76 pKa = 11.01 KK77 pKa = 10.34 QVSKK81 pKa = 9.67 TCNLRR86 pKa = 11.84 YY87 pKa = 9.59 FISCEE92 pKa = 3.91 SGEE95 pKa = 4.5 GKK97 pKa = 10.37 GKK99 pKa = 10.53 RR100 pKa = 11.84 GFANNSHH107 pKa = 4.67 YY108 pKa = 10.76 HH109 pKa = 5.57 ILFFLFPKK117 pKa = 9.93 EE118 pKa = 4.32 GKK120 pKa = 9.89 SLLQPYY126 pKa = 7.46 EE127 pKa = 3.57 FRR129 pKa = 11.84 HH130 pKa = 4.86 YY131 pKa = 9.92 VRR133 pKa = 11.84 KK134 pKa = 9.19 YY135 pKa = 7.54 WQGFDD140 pKa = 3.43 EE141 pKa = 4.96 SVCFEE146 pKa = 4.4 DD147 pKa = 6.33 FRR149 pKa = 11.84 NCKK152 pKa = 10.11 YY153 pKa = 10.92 GIARR157 pKa = 11.84 EE158 pKa = 3.91 GDD160 pKa = 3.31 NLGEE164 pKa = 4.17 VTSINALQYY173 pKa = 8.59 VVKK176 pKa = 10.82 YY177 pKa = 6.97 VTKK180 pKa = 10.45 DD181 pKa = 3.19 LNSISNEE188 pKa = 3.33 KK189 pKa = 10.53 KK190 pKa = 9.87 IFDD193 pKa = 3.87 RR194 pKa = 11.84 YY195 pKa = 10.05 EE196 pKa = 3.99 SYY198 pKa = 11.26 LNKK201 pKa = 9.83 ISYY204 pKa = 9.7 LPKK207 pKa = 10.29 SYY209 pKa = 11.05 SDD211 pKa = 3.8 YY212 pKa = 11.43 VDD214 pKa = 3.23 TFFNGAVLVPNFDD227 pKa = 3.1 FATRR231 pKa = 11.84 LFRR234 pKa = 11.84 RR235 pKa = 11.84 YY236 pKa = 9.18 IHH238 pKa = 5.67 TLKK241 pKa = 10.69 SKK243 pKa = 10.32 YY244 pKa = 7.24 NTRR247 pKa = 11.84 LVSRR251 pKa = 11.84 SNLLLLINEE260 pKa = 4.4 EE261 pKa = 4.28 YY262 pKa = 11.02 KK263 pKa = 11.16 NFLISTKK270 pKa = 10.59 KK271 pKa = 10.35 KK272 pKa = 8.62 ICFDD276 pKa = 3.33 EE277 pKa = 5.25 FIIRR281 pKa = 11.84 EE282 pKa = 4.0 ASLLTFFEE290 pKa = 3.93 QWRR293 pKa = 11.84 RR294 pKa = 11.84 QQVSEE299 pKa = 4.24 KK300 pKa = 10.12 LQEE303 pKa = 3.92 FKK305 pKa = 10.95 KK306 pKa = 10.42 IFRR309 pKa = 11.84 NRR311 pKa = 11.84 YY312 pKa = 6.8 SSKK315 pKa = 9.97 PRR317 pKa = 11.84 FSHH320 pKa = 6.17 GLGKK324 pKa = 10.22 IGLDD328 pKa = 3.67 TVDD331 pKa = 2.98 KK332 pKa = 10.4 SYY334 pKa = 10.92 PIFVDD339 pKa = 3.46 PCTNKK344 pKa = 9.74 PVRR347 pKa = 11.84 IPLYY351 pKa = 9.05 YY352 pKa = 10.11 FRR354 pKa = 11.84 HH355 pKa = 6.52 LFTDD359 pKa = 3.88 VKK361 pKa = 10.57 KK362 pKa = 11.1 LNGNNVYY369 pKa = 10.45 VLNEE373 pKa = 4.1 DD374 pKa = 3.66 GIKK377 pKa = 10.58 YY378 pKa = 10.11 KK379 pKa = 10.49 EE380 pKa = 4.23 SKK382 pKa = 10.65 LSEE385 pKa = 4.16 KK386 pKa = 10.71 LNYY389 pKa = 10.34 LADD392 pKa = 3.69 QLIKK396 pKa = 10.73 SFYY399 pKa = 10.06 AASKK403 pKa = 10.36 LPSDD407 pKa = 3.43 IQLLNFGRR415 pKa = 11.84 YY416 pKa = 8.27 IDD418 pKa = 3.36 IAFPNRR424 pKa = 11.84 KK425 pKa = 9.12 YY426 pKa = 10.98 VDD428 pKa = 3.38 DD429 pKa = 3.8 YY430 pKa = 12.12 ARR432 pKa = 11.84 FKK434 pKa = 11.0 LIYY437 pKa = 9.1 EE438 pKa = 4.16 GRR440 pKa = 11.84 KK441 pKa = 9.35 FPISEE446 pKa = 4.22 FNNPNLLDD454 pKa = 4.49 DD455 pKa = 4.15 SLTYY459 pKa = 10.33 RR460 pKa = 11.84 SNLFCSAWYY469 pKa = 9.04 FPPGLVTDD477 pKa = 4.11 YY478 pKa = 11.34 NPDD481 pKa = 3.56 YY482 pKa = 11.2 LEE484 pKa = 4.33 YY485 pKa = 10.33 AYY487 pKa = 10.41 HH488 pKa = 7.56 PYY490 pKa = 10.24 FLQNIEE496 pKa = 4.11 LFSYY500 pKa = 10.52 FDD502 pKa = 4.78 RR503 pKa = 11.84 ILSYY507 pKa = 10.25 FANNKK512 pKa = 8.84 NVVYY516 pKa = 8.52 WQKK519 pKa = 11.1 YY520 pKa = 8.17 NDD522 pKa = 3.32 WKK524 pKa = 9.69 KK525 pKa = 8.29 TKK527 pKa = 10.58 NYY529 pKa = 9.78 INSLSII535 pKa = 3.67
Molecular weight: 63.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.627
IPC2_protein 8.682
IPC_protein 8.551
Toseland 8.96
ProMoST 8.96
Dawson 9.37
Bjellqvist 9.209
Wikipedia 9.575
Rodwell 9.487
Grimsley 9.458
Solomon 9.385
Lehninger 9.326
Nozaki 9.18
DTASelect 9.136
Thurlkill 9.194
EMBOSS 9.458
Sillero 9.355
Patrickios 3.973
IPC_peptide 9.37
IPC2_peptide 7.907
IPC2.peptide.svr19 7.753
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1686
98
580
337.2
38.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.567 ± 1.163
0.949 ± 0.331
5.516 ± 0.417
5.219 ± 0.633
6.168 ± 1.102
4.686 ± 0.904
1.839 ± 0.378
6.999 ± 1.148
6.821 ± 0.995
9.075 ± 0.584
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.246 ± 0.411
7.711 ± 0.986
3.321 ± 0.464
4.152 ± 1.06
4.508 ± 0.562
10.202 ± 1.165
5.279 ± 0.57
5.219 ± 0.512
0.712 ± 0.192
5.813 ± 1.591
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here